• Applied Biological Chemistry
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• 제31권4호
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• pp.382-386
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• 1988

Chemically defined medium을 사용하여 Bacillus sp. $T_2-3$으로 균체외단백질을 생산한 후 이들의 각종 물리화학적 성질을 조사 하였다. 균체외단백질은 전기영동과 gel 여과 결과로 분자량이 약 49,000이 되는 비슷한 2종의 단백질로 구성되어 있었다. 최대흡수파장은 230nm 부근이었으며 aspartic acid를 가장 많이 함유하고 있었다. 또한 균체외단백질의 물에대한 용해도는 55.8%, 0.4% NaOH에 대한 용해도는 28.4%로 albumin과 glutelin계통의 단백질로 생각되었다.

• Journal of Microbiology and Biotechnology
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• 제10권1호
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• pp.103-106
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• 2000

Bacillus cereus KCTC 3674 excretes at least two kinds of extracellular proteases into the growth medium. Two major bands of the protease activity with molecular weights of approximately 100 and 38 kDa were obtained after gelatin-SDS-PAGE. The protease with a molecular weight of 38kDa was identified as an extracellular neutral (metallo-) protease. The neutral protease was quite thermostabile but labile to alkaline pH. On the contrary, the 100-kDa protease was thermolabile but stable to alkaline pH. The production of 38-kDa neutral protease was strongly affected by temperature, oxygen, carbonylcyanied m-chlorophenylhydrazone(CCCP) that was defined as a protonophofre, and cerulenin which inhibited lipid synthesis and caused changes in the membrane composition. On the other hand, the production of the 100-kDa protease was strongly affected by only temperature and cerulenin. Quinacrine (0.2 mM), which inhibits the penicillinase-releasing proteases of Bacillus licheniformis, had no effect, whatsoever, on the production of extracellular proteases in B.cereus KCTC 3674.

• 한국정보통신학회논문지
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• 제4권5호
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• pp.985-996
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• 2000

해안 지역에서 해양으로부터 들어오는 중규모 정도의 강수는 단기 예보를 위하여 원격 탐사에 의한 실시간 관측이 이루어져야 한다. 그러나 위성 관측은 강수를 상세히 묘사하지 못하기 때문에 레이더를 이용한 원거리관측이 요구된다. 본 연구에서는 레이더로 관측된 자료가 강수역을 정확하게 정량화 할 수 있도록 원거리 관측자료의 보정을 논의한다. 반사도의 연직 변화에 기인한 오차는 평균 분포나 통계적 방법으로 그리고 반사도의 감쇠 효과는 반복 편광법으로 보정될 수 있다. 이 같은 다양한 보정을 통하여 레이더로 관측된 원거리 자료를 유용화할 수 있다.

• Journal of Microbiology and Biotechnology
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• 제1권4호
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• pp.274-280
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• 1991

Effects of IPTG induction on cell growth and production of ${\beta}$-galactosidase-preS2 fusion protein (${\beta}$gal-preS2) were studied in a defined medium using a recombinant Escherichia coli JM109/pCMHB30. IPTG was added (0.2 mM) to induce the cloned-gene expression in the early-, mid-, and late-log growth phases. The most serious decreases in growth rate and plasmid stability were observed for the induction in the early-log growth phase. The expression level of ${\beta}$gal-preS2 attained by the induction in the mid-log phase was about 0.51 mg fusion protein/mg total cellular protein, which was 2- and 5-fold improvement over the levels obtained with the inductions in the early- and late-log phases. Formation of acidic byproducts including acetate and pyruvate showed different profiles during the fermentation period for each cases of induction; pyruvate was the major byproduct for the induction in the early-log phase while acetate production became more significant for the cases of inductions in the mid- and late-log phases.

• 한국통신학회논문지
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• 제24권9A호
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• pp.1425-1433
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• 1999

본 논문은 ETSI에 의해 정의된 무선 LAN(Wireless LAN)의 표준안인 HIPERLAN(HIgh PErformance Radio LAN) 타입 1의 매체 접근 제어 프로토콜에 관하여 해석적으로 분석한다. HIPERLAN의 채널 접근 계층(Channel Access Control layer)에서 사용하는 EY-NPMA(Elimination Yield-Nonpreemptive Priority Multiple Access)의 채널 경쟁 단계 중에서 제어 단계(Elimination phase)와 양보 단계(Yield phase)의 무선 가입자 모델에 대하여 분석한다. 그리고, 분석 결과를 이용하여 전송 단계(Transmission phase)에서 패킷 전송의 성공확률을 해석적으로 구한 후, 시뮬레이션을 이용한 성능 평가를 통하여 분석 결과를 검증한다.

• 대한조선학회 특별논문집
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• 대한조선학회 2017년도 특별논문집
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• pp.93-104
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• 2017

IMO type C independent tank is one of the cargo containment system specified on IGC code. It is normally adopted for small and medium size liquefied gas carrier's cargo containment system and it can be applied to fuel tank of LNG fueled vessel. This study focuses on rule scantling process and evaluation methods in early design stage of type C independent tank. Actual design results of 22K LPG/Ammonia/VCM carrier's No.2 cargo tank are demonstrated. This paper presents the calculation methods of design acceleration and liquid height for internal design pressure as defined on IGC code. And this paper shows the applied results of classification rules about shell thickness requirement and buckling strength. Additionally this paper deals with evaluation methods of structural strength and cumulative fatigue damage using FE analysis.

• 천문학회보
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• 제39권2호
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• pp.78.1-78.1
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• 2014

An ionization front (IF) surrounding an H II region is a sharp interface through which a cold neutral gas makes transition to a warm ionized phase by absorbing UV photons from central massive stars. We investigate the structure and instability of a plane-parallel D-type IF threaded by magnetic fields parallel to the front. We find that magnetic fields increase the maximum propagation speed of the IFs, while reducing the expansion factor, defined as the density ratio of neutral to ionized phases. IFs become unstable to distortional perturbations due to gas expansion across the fronts, exactly analogous to the Darrieus-Landau instability of ablation fronts in terrestrial flames. The growth rate of the IF instability is proportional linearly to the perturbation wavenumber as well as the upstream flow speed. The IF instability is stabilized by gas compressibility and becomes completely quenched when the front is D-critical. The instability is also stabilized by magnetic pressure when the perturbations propagate in the direction perpendicular to the fields. When the perturbations propagate in the direction parallel to the fields, on the other hand, it is magnetic tension that reduces the growth rate, completely suppressing the instability when ${\beta}$ < 1.5, with ${\beta}$ denoting the square of the ratio of the sound speed to the Alfven speed in the pre-IF region. When the front experiences an acceleration, the IF instability cooperates with the Rayleigh-Taylor instability to make the front more unstable. We discuss potential effects of IF instability on the evolution and dynamics of IFs in the interstellar medium.

• Journal of Communications and Networks
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• 제7권4호
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• pp.471-477
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• 2005

This paper proposes an efficient constraint-based optimization model for the design of 3G mobile networks, such as universal mobile telecommunications system (UMTS). The model concerns about finding a set of sites for locating radio network controllers (RNCs) from a set of pre-defined candidate sites, and at the same time optimally assigning node Bs to the selected RNCs. All these choices must satisfy a set of constraints and optimize an objective function. This problem is NP-hard and consequently cannot be practically solved by exact methods for real size networks. Thus, this paper proposes a hybrid search strategy for tackling this complex and combinatorial optimization problem. The proposed hybrid search strategy is composed of three phases: A constraint satisfaction method with an embedded problem-specific goal which guides the search for a good initial solution, an optimization phase using local search algorithms, such as tabu algorithm, and a post­optimization phase to improve solutions from the second phase by using a constraint optimization procedure. Computational results show that the proposed search strategy and the model are highly efficient. Optimal solutions are always obtained for small or medium sized problems. For large sized problems, the final results are on average within $5.77\%$ to $7.48\%$ of the lower bounds.

• Computers and Concrete
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• 제21권1호
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• pp.21-30
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• 2018

In this study, we analyze the behavior of concrete which contains zeolite and diatomite. In order to achieve the goal, we utilize expert system methods. The utilized methods are artificial neural network and adaptive network-based fuzzy inference systems. In this respect, we exploit seven different mixes of concrete. The concrete mixes contain zeolite, diatomite, mixture of zeolite and diatomite. All seven concrete mixes are exposed to 28, 56 and 90 days' compressive strength experiments with 63 specimens. The results of the compressive strength experiments are used as input data during the training and testing of expert system methods. In terms of artificial neural network and adaptive network-based fuzzy models, data format comprises seven input parameters, which are; the age of samples (days), amount of Portland cement, zeolite, diatomite, aggregate, water and hyper plasticizer. On the other hand, the output parameter is defined as the compressive strength of concrete. In the models, training and testing results have concluded that both expert system model yield thrilling medium to predict the compressive strength of concrete containing zeolite and diatomite.

• Architectural research
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• 제13권3호
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• pp.19-29
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• 2011

Visibility of a space have been defined in several different ways: such as the axial line covering a convex space, a convex space defining the fattest shape in a space and an Isovist field formed by a field of vision at a given vantage point. Isovist fields are referred to as a descriptive medium to describe a movement by reviewing and analyzing geometric properties in them. Many descriptive methods for analysis of three-dimensional isovist are applied to analyzing the morphological properties in a 3D space more realistically. Although these models are regarded as a more advanced method for describing spatial properties, they have pros and cons such as complex mathematical calculations and somewhat arbitrary calibration in addition to huge consumption of memory space. These difficulties lead to the development of a three-dimensional visual accessibility model that explores the implication of building shape on the calculation of isovist fields drawn on a 2D plane. We propose a conceptual framework of how to measure the isovist field not as a 3D volume but as a combination of 2D plane on the ground with the 3D building shape of it's facade.