• Advances in nano research
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• v.6 no.1
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• pp.81-92
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• 2018

Biosynthesis of nanoparticles has acquired particular attention due to its economic feasibility, low toxicity and simplicity of the process. Extracellular synthesis of nanoparticles by bacteria and fungi has been stated to be brought about by enzymes and other reducing agents that may be secreted in the culture medium. The present study was carried out to determine the underlying mechanisms of extracellular silver nanoparticle synthesis by Pseudomonas hibiscicola isolated from the effluent of an electroplating industry in Mumbai. Synthesized nanoparticles were characterized by spectroscopy and electron microscopic techniques. Protein profiling studies were done using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (1D-SDS PAGE) and subjected to identification by Mass Spectrometry. Characterization studies revealed synthesis of 50 nm nanoparticles of well-defined morphology. Total protein content and SDS PAGE analysis revealed a reduction of total protein content in test (nanoparticles solution) samples when compared to controls (broth supernatant). 45.45% of the proteins involved in the process of nanoparticle synthesis were identified to be oxidoreductases and are thought to be involved in either reduction of metal ions or capping of synthesized nanoparticles.

• The Korean Journal of Cytopathology
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• v.7 no.2
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• pp.218-224
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• 1996

We describe two cases of metastatic Wilms' tumor in the lung with emphasis on the cytologic features of specimens obtained by needle aspiration. One of them was extrarenal Wilms' tumor. The findings were correlated with the histopathologic features of the primary lesion. Cellular components in the fine needle aspiration cytology (FNAC) slides included blastemal, epithelial, stromal and inflammatory cells with immature tubular differentiation and rosette formation. Recognition of these cellular components in FNAC smears help in establishing FNAC diagnosis of Wilms' tumor. The blastemal cells were represented by small to medium sized cells with scanty cytoplasm having ill-defined borders and round to slightly oval nuclei with evenly dispersed chromatin and small marginated nucleoli. They were seen in our two cases. The differential diagnosis includes neuroblastoma, malignant lymphoma, malignant rhabdoid tumor, clear cell sarcoma, Ewing's sarcoma and embryonal rhabdomyosarcoma. In conclusion, making a definite cytologic diagnosis of metastatic Wilms' tumor may be possible by light and electron microscopy and immunohistochemical staining. The above findings may contribute to the diagnosis of FNAC of metastatic Wilms' tumor.

• Korean Journal of Animal Reproduction
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• v.19 no.2
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• pp.135-140
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• 1995

The study was conducted to investigate on the survival rate of bisected porcine embryos co-cultured in 10% FCS(v/v)＋TCM-199 media containing hormones, oviductal epithelial cells and cumulus cells 0 to 72 hrs after bisection. In vitro survival rate was defined as development rate on in vitro culture or FDA-test. The results are summarized as follows ; 1. The survival rate of bisected porcine embryos cultrued in 10% FCS＋TCM-199 media contaning PMSG, hCG, PMSG＋hCG, hCG＋$\beta$-estradiol 0 to 20 hrs and 20 to 40 hrs were 37.6% and 37.5%, 28.6% and 28.6%, 35.7% and 28.8%, 30.8% and 23.1%, 38.5% and 30.7%, respectively. The survival rate of bisected embryos co-cultured in TCM-199 media containing hormones, oviductal epithelial cells and cumulus cells was significantly higher than that of non co-culture. 2. The survival rate of bisected porcine embryos co-cultured 10% FCS＋TCM-199 media containing oviductal epithelial cells 4 to 5 hrs and 20 to 24 hrs were 42.9% and 38.5%, respectively. 3. The survival rate of bisected porcine embryos co-cultured in 10% FCS＋TCM-199 media containing cumulus cells 4 to 5 hrs and 20 to 24 hrs were 40.0% and 35.7%, respectively.

• KSBB Journal
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• v.19 no.4
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• pp.257-262
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• 2004

Recently, there is a growing interest in microalgae and the use of microalgae focused on the production of various high value metabolite used in food, pharmaceuticals and cosmetics. The key limiting factor in high density algal cultivation is the light and algal growth is defined by light intensity and light penetration depth into the culture medium. The effect of light with various light paths, S/V ratios, light intensities, and 50% duty cycle on the growth of microalgae was examined to enhance microalgal biomass productivity and photosynthetic efficiency. We confirmed that the utilization of efficient light energy was obtained from 4 cm of diameter, 57.6% of S/V ratio, 62 ${\mu}$mol/㎡/s of light intensity.

• Journal of Energy Engineering
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• v.21 no.1
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• pp.75-80
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• 2012

In this paper a realistic bounding method for flooding analysis following rupture of large size of thanks and piping is defined. Mass and energy release during main feedwater line break accident is analyzed with RETRAN code. It is modeled that the main feed water control valve is closed in 5.0 seconds after reactor trip. In result of the analysis, largest mass and energy is discharged at 70% reactor power. The flood sources for main feedwater room are calculated when piping failure occurs in the high energy line and medium energy line. Based on the result of flood level (1.43m), it is investigated that all of the safety-related environmental qualification equipments are well located above the flood level.

• Explosives and Blasting
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• v.16 no.2
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• pp.23-33
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• 1998

This study has been carried out to design the standards of rock bolts which are recommended to be used for supporting material in Seong Ju tunnel, Under recently planned to construct. Due to the weak bedding rocks along the Tunnel, NATM supporting method is assumed to be applied and the design and calculation for rock bolts which are important material for this supporting method. Though the study, the results obtained are as follows. 1) The rocks defined s coarse sandstone, fine sandstone, medium sandstone, sandy shale and silty shale are normal rocks in strength, and black shale, greywacke and conglomerate are weak rocks in strength. 2) When the length of 3∼4meters of rock bolts are used, the distances of bolts are supposed to be 1.3∼1.8m in sandstone and 1.3∼2.0m in shale and for greywacke the distance should be 1.5m.

• Journal of Information Technology Services
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• v.17 no.1
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• pp.59-77
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• 2018

Software process (SP) authentication is a certification system designed in order to improve software process and ensure software reliability of the Korean small and medium enterprises (SMEs). It is composed of 3 grades, 17 evaluation items and 70 detailed evaluation items (software process). In order to certificate grade 2, 42 detailed evaluation items must be satisfied. In order to certificate grade 3, all 70 detailed evaluation items must be satisfied. SMEs are more vulnerable to manpower and material resources than the major company. Therefore, it is difficult for SMEs to invest in the long term to improve the software process. If we can provide the core evaluation items to SMEs, SMEs will be able to improve software quality more efficiently and effectively. It can be used as a short way to acquire SP authentication. The evaluation items of SP are defined as influencing each other. Therefore, the core evaluation items might be established using related techniques. Although we searched for previous researches and related informations, the associated study was insufficient. In this study, ANP (Analytic Network Process) technique was applied, and the core evaluation items of SP authentication were established. SMEs will be able to acquire SP authentication more easily using the core evaluation items. Finally, we expect that this study will be used as a basis material for creating the SP 2.0 version.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.04a
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• pp.80-104
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• 1996

The development of routine techniques for the isolation and in vitro maintenance of conducting airway epithelial cells in a differentiated state provides an ideal model to study the factors involved in the regulation of the expression of mucocilicary differentiation. Several key factors and conditions have been identified. These factors and conditions include the use of biphasic culture technique to achieve mucociliary differentiation and the use of such stimulators, the thickness of collagen gel substratum, the calcium level, and vitamin A, and such inhibitors, the growth factors EGF and insulin, and steroid hormones, for mucous cell differentiation. Using the defined culture medium, the life cycle of the mucous cell population in vitro was investigated. It was demonstrated that the majority of the mucous cell population in primary cultures is not involved in DNA replication. However, the mucous cell type is capable of self-renewal in culture and this reproduction is vitamin A dependent. furthermore, differentiation from non-mucous cell type to mucous cell type can be demonstrated by adding back a positive regulator such as vitamin A to the “starved” culture. Cell kinetics data suggest that vitamin A-dependent mucous cell differentiation in culture is a DNA replication-independent process and the process is inhibited by TGF-${\beta}$1.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.14 no.3
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• pp.453-462
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• 1994

Variation of seismic wave field in a multi-layered attenuating elastic half space is studied by the propagator matrix method and point source models of which fault-slip functions are defined as ramp functions. In this paper, the earth is modeled as being composed of horizontally stratified layers, with uniform material properties for each layer. The partial differential equations for the seismic motion in each layer are solved using a Fourier Hankel transform approach. Time histories and frequency contents of accelerations and displacements due to a vertical dip-slip and strike-slip point source located in the underlain half space are calculated at the layer interfaces using the developed programs and their characteristics are represented.

• Journal of Microbiology and Biotechnology
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• v.5 no.2
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• pp.61-67
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• 1995

To investigate the role of induction on CGTase production for alkalophilic Bacillus firm us var. alkalophilus H609, the constitutive mutants that form a halo around its colonies at non-inducible AG agar media containing amylose and glucose were selected. The selected constitutive mutants could produce CGTase in the range of 18.9 to 28.8 units/ml $\cdot A_{600}$ in the alkaline basal medium, and finally a constitutive mutant Bacillus firmus var. alkalophilus CM46 was selected. The constitutive nature of CM46 was also confirmed in protein level using SDS-PAGE. The effects of induction and catabolite repression for both parent strain Bacillus firmus var. alkalophilus H609 and constitutive mutant CM46 were also compared by adding soluble starch and glucose during cultivation. The selected mutant CM46 was a non-inducible but a catabolite regulated type mutant. Even though inductive regulation was released, the specific CGTase activity defined as CGTase activity per cell concentration was not increased compared with that of parent strain. The cell growth and CGTase production patterns of constitutive mutant Bacillus firmus var. alkalophilus CM46 were compared with the parent strain to identify CGTase production characteristics.