• Journal of Microbiology and Biotechnology
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• 제15권4호
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• pp.689-693
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• 2005

Bacillus sp. JB-99, when grown in a chemically defined medium containing lactose as a carbon source, yielded 3,860 U/ml extracellular $\beta$-mannanase, which was high compared to other examined carbon sources. Among the nitrogen sources, yeast extract enhanced the enzyme activity. The enzyme production was growth-associated. The enzyme was optimally active at $65^{\circ}C$, pH 10, and had a half-life of 190 min at $65^{\circ}C$. N-Bromosuccinamide and $AgNO_3,\;CuSO_4$, and $HgCl_2$ strongly inhibited the enzyme, whereas $Ca^{2+}$ stimulated the enzyme activity. The $\alpha$-galactosidase enzyme production was not found in any of the enzyme assays.

• 한국가축번식학회지
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• 제9권2호
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• pp.153-157
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• 1985

Ejaculated and epidiymal goat spermatozoa were preserved for 0, 6, 12 adn 18 h, and 0 and 18 h in a semi-aerobic condition at 20-$25^{\circ}C$, and preincubated for 5-6 h in a CO2 incubator in m-KRB solution. Then they were preincubated at different concentrations (3-5, 25-48 and 105-190$\times$107/ml), and ability of penetration into zona-free hamster eggs in vitro was examined. When ejaculated spermatozoa were preincubated in m-KRB solution after presservation for 12 and 18 h, 12 and 29% of zona-free eggs were penetrated, and only 4% of eggs were penetrated by epididymal spermatozoa which were preincubated after preservation for 18 h. When spermatozoa were preincubated at a low concentration, the penetration rates were very low. But when the sperm concentration during preincubation was 25-48 and 105-190$\times$107/ml, the penetration rates increased to about 30%.

• 한국경영과학회지
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• 제22권3호
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• pp.145-162
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• 1997

This paper an analytical model for estimating packet waiting times at stations in the DQDB network, where the most upstream station is saturated. This model is useful in comparing the extreme unfairness which downstream stations experience due to their geographical locations in accessing the medium. Each station is modeled as an M/G/1, where the service time is defined to be the time a packet spends in the transmission buffer. The service time is decomposed into five components, and in turn, the first and second moment of each component are derived in three different modes of operation. Simulation experiments are presented for model validation and results are discussed.

• Structural Engineering and Mechanics
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• 제64권6권
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• pp.683-693
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• 2017

According to a generalized nonlocal strain gradient theory (NSGT), dynamic modeling and free vibrational analysis of nanoporous inhomogeneous nanoplates is presented. The present model incorporates two scale coefficients to examine vibration behavior of nanoplates much accurately. Porosity-dependent material properties of the nanoplate are defined via a modified power-law function. The nanoplate is resting on a viscoelastic substrate and is subjected to hygro-thermal environment and in-plane linearly varying mechanical loads. The governing equations and related classical and non-classical boundary conditions are derived based on Hamilton's principle. These equations are solved for hinged nanoplates via Galerkin's method. Obtained results show the importance of hygro-thermal loading, viscoelastic medium, in-plane bending load, gradient index, nonlocal parameter, strain gradient parameter and porosities on vibrational characteristics of size-dependent FG nanoplates.

• Journal of Microbiology and Biotechnology
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• 제2권2호
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• pp.129-134
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• 1992

Segregational instability of a recombinant plasmid, pDML6, encoding extracellular $\beta$-lactamase in Streptomyces lividans PD6 was characterized by growth kinetic analysis. The quantitative determination of the plasmid harbored in the mycelia was evaluated with mycelia fragmented mechanically, and also with colonies regenerated from protoplasts. Conditions for the formation of protoplasts and regeneration of protoplasts were established. The maximal specific growth rates of the host strain and the plasmid-harboring strain in a chemically defined medium without selection pressure were the same. The probability of plasmid loss from the harbouring cells was higher at higher growth rates. Mathematical models for the prediction of cell growth, substrate uptake, and accumulation of the cloned gene product were developed.

• Steel and Composite Structures
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• 제1권3호
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• pp.283-294
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• 2001

In the present work a constitutive model is developed which permits the assessment of the structural performance through a criterion based on cumulative damage. For it, a damage index is defined and is evaluated through the application of the Miner's rule in low-cycle fatigue. However, the damage index is not considered as a posteriori variable since is incorporated explicitly as an internal variable in the constitutive equations which produces a direct coupling between the damage and the structural mechanical behaviour allowing the possibility of considering as a whole different coupled phenomena. For the elaboration of this damage model, the concepts of the mechanics of continuum medium are applied on lumped dissipative models in order to obtain a coupled simplified model. As a result an elastoplastic model coupled with damage and fatigue damage is obtained.

• International Journal of Precision Engineering and Manufacturing
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• 제5권2호
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• pp.5-15
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• 2004

A telecooperation system supports synchronous cooperation among all the product development partners and enables compensation of deficits concerning traditional information/communication technologies. By analyzing the product development processes and communication scenarios of a company manufacturing automobile parts, the specifications for the suitable telecooperation system were defined. An integrated telecooperation platform that combines the commercial softwares with the new developed modules for customer-oriented functionalities was created as a product development guideline. Future work is strongly required in order to define the best way to improve the integrated platform, based on the functionality of ERP and PDM systems.

• 대한기계학회논문집B
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• 제23권6호
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• pp.713-721
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• 1999

A composite plastic, where long metallic fibers are used as filling materials, is transformed from nonconducting to conducting medium as the volume fraction of filling metallic fibers is increased from zero : such drastic change in property is called the percolation. It is desired both for practical and theoretical purposes to understand the physics underlying the percolation and to estimate the percolation threshold that is defined by the minimum volume fraction of the metallic fibers for which the percolation occurs. In this study, percolation thresholds are calculated by Monte Carlo Computer simulation. Both lattice and continuum spaces are considered and detailed microstructures of metallic fibers are modelled as rigid and flexible bodies for both model spaces. Simulations are carried out for wide range of aspect ratios and discussions are given.

• 대한기계학회논문집B
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• 제25권10호
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• pp.1293-1302
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• 2001

It is generally known that radial vane blowers with vaneless diffuser may generate mostly only a rotating stall but backward curved vane blowers may do both an impeller and a diffuser rotating stalls. In this study, it was found from the numerical and experimental results that the diffuser rotating stall does not appear in a radial vane because of the suppression for the diffuser stall appearance by occurring of impeller rotating stall in a large flow rate coefficient. The diffuser rotating stalls occurring when the width of diffuser is broaden fur a backward curved vane blower are classified definitely by the diffuser flow rate coefficient defined by adopting the varying diffuser width.

• 한국정밀공학회지
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• 제16권8호
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• pp.162-169
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• 1999

Fourier heat conduction law becomes invalid for the situations involving extremely short time heating, very low temperatures and fast moving heat source(or crack), since the wave nature of heat propagation becomes dominant. For these conditions, the modified heat conduction equation with the finite propagation speed of heat in the medium could be applied to predict heat flux and temperature distributions. In this study, temperature distributions at the vicinity of a very fast moving heat source are investigated numerically. Thermal fields are characterized by thermal Mach numbers(M) defined as the ratio of moving heat source speed to heat propagation speed in the solid. In the transonic and supersonic ranges($M{\ge}1$), thermal shocks are shown, which separate the heat affected zone from the thermally undisturbed zone.