• Journal of Microbiology and Biotechnology
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• v.14 no.1
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• pp.90-96
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• 2004

A synthetic defined medium, fortified with amino acids, was developed for the stable production of the kringle fragments of human apolipoprotein(a) (apo(a)), rhLK68. Using a complex rich medium containing yeast extract and a high-cell-density fed-batch culture, the expression level of rhLK68 reached 17% of the total cellular protein, which corresponded to $5\;g\;l^{-1}$ of the culture. To replace the complex media with chemically defined media, several amino acids that positively affect cell growth and gene expression were chosen by a statistical method. The various combinations of the selected amino acids were tested for its fortifying effect on a minimal defined medium. When glutamine only was added, the overall expression level of rhLK68 reached 93% of the complex rich medium increasing the specific expression level by 22.4% and decreasing the cell growth by 24%. Moreover, the addition of glutamine resulted in a 2-fold increase in the concentration of rhLK68 in the culture broth, compared with the minimal defined medium. The synthetic defined media developed in this study could be generally applied to high-cell-density cultures of the recombinant Escherichia coli BL21(DE3), especially for the production of therapeutic proteins that require a strict quality control of the culture media and fermentation processes.

• Smart Media Journal
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• v.5 no.1
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• pp.69-77
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• 2016

This paper proposes a software defined storaging method to converge the network virtualization technique and the RAID of distributed storage environment. The proposed method designs software based storage which it apply a flexible control and maintenance of storages. In addition, the method overcomes the restricted of physical storage capacity and cut costs of data recovery. The proposed failover recovery technique based on Software-Defined RAID has been tested the substantial verification and the performance using public AWS and Google Storage.

• Proceedings of the Korean Information Science Society Conference
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• 2005.07a
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• pp.604-606
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• 2005

셋탑 박스 (Settop Box)는 다양한 가전 간의 통신을 통해 디지털 멀티미디어 서비스를 구현하는데 있어 중요한 홈 네트워킹 기술 요소 중 하나이다. 셋탑 박스의 구현을 위하여 다양한 플렛폼에서 개발이 진행되는 가운데 본 논문에서는 SDMP(Software Defined Media Platform)에 기반하여 멀티미디어를 제작하고 스트리밍 서비스를 할 수 있는 홈 네트워킹 용 셋탑 박스를 구현하였다. 본 논문에서는 다양한 인터페이스를 갖는 가전 기기를 지원할 수 있는 엑스스케일 보드 (Xscale board)를 제작하고 임베디드 리눅스 (Embedded Linux)를 포팀하여 실시간으로 인코딩된 멀티미디어를 스트리밍할 수 있는 서비스 구현의 예를 제시하였다.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2004.09a
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• pp.25-28
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• 2004

The capillary number is used to represent the mobilization potential of organic phase trapped within porous media. The capillary number has been defined by three different forms, according to types of flow velocity and viscosity used in the definition of capillary number. This study evaluated the suitability of the capillary number definitions for representing TCE mobilization by constructing capillary number-TCE saturation relationships. The results implied that the capillary number should be correctly employed, according to interest of scale and fluid flow behavior. This study suggests that the pore-scale capillary number may be used only for investigating the organic-phase mobilization at the pore scale because it is defined by the pore-velocity and the dynamic viscosity. The Newtonian-fluid capillary number using Darcy velocity and the dynamic viscosity may be suitable to quantify flood systems representing Newtonian fluid behavior. For viscous-force modified flood systems such as surfactant-foam floods, the apparent capillary number definition employing macroscopic properties (permeability and potential gradient) may be used to appropriately represent the desaturation of organic-phases from porous media.

• Journal of Intelligence and Information Systems
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• v.24 no.2
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• pp.37-57
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• 2018

With the spread of smart phones, interest in mobile media is on the increase as useful media recently. Mobile media is assessed as having differentiated advantages from existing media in that not only can they provide consumers with desired information anytime and anywhere but also real-time interaction is possible in them. So far, studies on mobile advertising were mostly researches analyzing satisfaction with, and acceptance of, mobile advertising based on survey, researches focusing on the factors affecting acceptance of mobile advertising messages and researches verifying the effect of mobile advertising on brand recall, advertising attitude and brand attitude through experiments. Most of the domestic mobile advertising studies related to advertisement effect and advertisement attitude have been conducted through experiments and surveys. The advertising effectiveness measure of the mobile ad used the attitude of the advertisement, purchase intention, etc. To date, there have been few studies on the effects of mobile advertising on actual advertising data to prove the characteristics of the advertising platform and to prove the relationship between the factors influencing the advertising effect and the factors. In order to explore advertising effect of mobile advertising platform currently commercialized, this study defined advertising characteristics and media characteristics from the perspective of advertiser, advertising platform and publisher and analyzed the influence of each characteristic on advertising effect. As the advertisement characteristics, we classified advertisement format classified by bar type and floating type, and advertisement material classified by image and text. We defined advertisement characteristics of advertisement platform as Hedonic and Utilitarian media characteristics. As a dependent variable, we use CTR, which is the ratio of response (click) to ad exposure. The theoretical background and the analysis of the mobile advertising business, the hypothesis that the advertisement effect is different according to the advertisement specification, the advertisement material, In the ad standard, bar ads are classified as static framing, Floating ads can be categorized as dynamic framing, and the hypothetical definition of floating advertisements, which are high-profile dynamic framing ads, is highly responsive. In advertising, images with high salience are defined to have higher ad response than text. In the media characteristics classified as practical / hedonic type, it is defined that the hedonic type media has a more relaxed tendency than the practical media, and there is a high possibility of receiving various information because there is no clear target. In addition, image material and hedonic media are defined to be highly effective in the interaction between advertisement specification and advertisement material, advertisement specifications and media characteristics, and advertisement material and media characteristics. As the result of regression analysis on each characteristic, material standard, which is a characteristic of mobile advertisement, and media characteristics separated into 'Hedonic' and 'Utilitarian' had significant influence on advertisement effect and mutual interaction effect was also confirmed. In the mobile advertising standard, the advertising effect of the floating advertisement is higher than that of the bar advertisement, Floating ads were more effective than text ads for image ads. In addition, it was confirmed that the advertising effect is higher in the practical media than the hedonic media. The research was carried out with the big data collected from the mobile advertising platform, and it was possible to grasp the advertising effect of the measure index standard which is used in the practical work which could not be grasped in the previous research. In other words, the study was conducted using the CTR, which is a measure of the effectiveness of the advertisement used in the online advertisement and the mobile advertisement, which are not dependent on the attitude of the ad, the attitude of the brand, and the purchase intention. This study suggests that CTR is used as a dependent variable of advertising effect based on actual data of mobile ad platform accumulated over a long period of time. The results of this study is expected to contribute to establishment of optimum advertisement strategy such as creation of advertising materials and planning of media which suit advertised products at the time of mobile advertisement.

• Asian Journal for Public Opinion Research
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• v.2 no.4
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• pp.228-247
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• 2015

Politics is defined as who gets what, when, and how. Communication is defined as the process, outcomes and effects of message transmission through a medium. Thus political communication in Asia, the most dynamic and uncertain region of the world, has been very dense, requiring rigorous scientific analysis and culturally sensitive reflection. Mass media brought about a revolution in political communication in the last century. In the 21st century, complex monitory devices from the state and international organizations and from the society and transnational groups have transformed the features of political communication. It has triggered another revolution in political communication. Illustrations are given focusing on Asia. A theoretical reflection from the angle of political science is attempted focusing on political communication medium and its effects. I compare no medium (face-to-face communication), mass media, and social media in relation to their effects.

• Journal of Microbiology and Biotechnology
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• v.13 no.4
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• pp.560-563
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• 2003

During our previous studies on the relationship between nutrient requirements of S. hygroscopicus C9 and rapamycin biosynthesis, we developed chemically-defined media containing among other nutrients, aspartic acid, arginine, histidine, or ammonium sulfate. However, these media (“Cheng et al. medium” and “Lee et al. medium”) showed very slow growth characterized by a very long lag phase of growth. In an attempt to develop a chemically-defined or semi-defined medium to support more rapid growth and increased cell production, we have carried out studies to shorten the lag phase. Of the various additives tested, vitamin-free casein acid hydrolysate was the most significant by shortening the lag phase by 2-3 days. Mixtures of amino acids failed to replace casein acid hydrolysate. The active principle passed through an ultrafilter with a molecular weight cutoff of 1,000 and thus may be a peptide. The present work has yielded a semi-defined medium which should be useful for further growth studies on S. hygroscopicus C9.

• Knowledge Management Research
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• v.16 no.3
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• pp.81-102
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• 2015

With the rapid development of digital media, there has been a huge change in a way of communication, a process of information diffusion and a role of traditional media. Not like mass media, social media enables users to generate and tap into the opinions of a larger world. From that reason, social media is impacting marketing strategies. However, still social media marketing researches just focus on case study, analysis of users motivation or analysis of power user's usage pattern. Word-of-mouth has always been important especially in marketing area. In social media, word-of-mouth depends on each user that's why this research focuses on individual user's activity in SNS. I defined 4 factors (produce, diffusion, network size, activity of network size enlarge) that are effect on activity and verified hypothesis by multiple regression analysis, hierarchical regression analysis and moderated multiple regression.

• Knowledge Management Research
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• v.16 no.2
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• pp.91-111
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• 2015

Social media marketing has gained attention from marketers due to the growing number of social media users. Marketers around the globe have a serious concern over how to utilize social media as a successful marketing tool. Many of them show a lack of understanding of the essential feature of social media and effective social media marketing strategies, which brings about a higher chance of social media marketing failures. Analyzing the failure cases of social media marketing is vital because it provides rich insights for marketing experts who have difficulty in developing effective social media marketing strategies. Therefore, this study conducted multiple case studies by selecting five failure cases of social media marketing which we defined as paradigmatic social media marketing failures which happened in the last five years in South Korea. From the case studies, we derived five successful social media marketing strategies. This study has a theoretical implication because it is the first to suggest effective social media marketing strategies based on the analysis on social media marketing failure cases. It also has a practical implication since it proposes specific social media marketing strategies which can help facilitate successful social media marketing.

• Journal of Digital Convergence
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• v.6 no.3
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• pp.75-84
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• 2008

The purpose of our this research, based on the Media Selection Theory, the Technology Acceptance Model, and the Social Influence Theory, is to investigate the influential factors that affect media selection in banking transactions. Analyses showed that for location sensitive bank window's and ATMs (automatic teller machines), defined as offline-based transaction channels, convenience was the variable affecting media selection. However, in the case of online media not related to location, (phone banking, internet banking, and mobile banking) reliability was the significant variable influencing use. The findings show that banking organizations may benefit from identifying traits of media affecting use, and should differentiate customer services for competitive advantage.