• Transactions of the Korean Society of Mechanical Engineers A
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• v.30 no.4 s.247
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• pp.435-441
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• 2006

The anterior cruciate ligament(ACL) is an important stabilizer of knee joint. The ACL injury of knee is common and a serious ACL injury leads to ligament reconstruction surgery. Gait analysis is essential to identify knee condition of patients who display abnormal gait. The purpose of this study is to evaluate and classify knee condition of ACL deficient patients using a nonlinear dynamic method. The nonlinear method focuses on understanding how variations in the gait pattern change over time. The experiments were carried out for 17 subjects(l2 healthy subjects and five subjects with unilateral deficiency) walking on a motorized treadmill for 100 seconds. Three dimensional kinematics of the lower extremity were collected by using four cameras and KWON 3D motion analysis system. The largest Lyapunov exponent calculated from knee joint flexion-extension time series was used to quantify knee stability. The results revealed the difference between healthy subjects and patients. The deficient knee was significantly unstable compared with the contralateral knee. This study suggests an evaluation scheme of the severity of injury and the level of recovery. The proposed Lyapunov exponent can be used in rehabilitation and diagnosis of recoverable patients.

• Molecules and Cells
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• v.25 no.1
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• pp.131-137
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• 2008

Crk-associated substrate (CAS) is a focal adhesion protein that is involved in integrin signaling and cell migration. CAS deficiency reduces the migration and spreading of cells, both of which are processes mediated by Rac activation. We examined the functions of v-Crk, the oncogene product of the CT10 virus p47gag-crk, which affects cell migration and spreading, membrane ruffling, and Rac activation in CAS-deficient mouse embryonic fibroblasts (CAS-/- MEFs). CAS-/- MEFs showed less spreading than did CAS+/+ MEFs, but spreading was recovered in mutant cells that expressed v-Crk (CAS-/-v-Crk MEF). We observed that the reduction in spreading was linked to the formation of membrane ruffles, which were accompanied by Rac activation. In CAS-/- MEFs, Rac activity was significantly reduced, and Rac was not localized to the membrane. In contrast, Rac was active and localized to the membrane in CAS-/-v-Crk MEFs. Lamellipodia protrusion and ruffle retraction velocities were both reduced in CAS-/- MEFs, but not in CAS-/-v-Crk MEFs. We also found that microinjection of anti-gag antibodies inhibited the migration of CAS-/-v-Crk MEFs. These findings indicate that v-Crk controls cell migration and membrane dynamics by activating Rac in CAS-deficient MEFs.

• Journal of Sasang Constitutional Medicine
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• v.22 no.3
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• pp.59-66
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• 2010

1. Objectives: The objectives of this paper are to report Traditional Chinese Medicine (TCM) Constitution Theory and the present state of Chinese Health Administration Center. 2. Methods: We visited China for "China Technology Survey" in 2009. Our purposes were to research for the study of constitutional medicine in China and to search for the Development Plan of Constitutional Medicine Through the Research of Chinese Constitutional Medicine. This paper is the report of the "China Technology Survey". 3. Results & Conclusions: In China, the concept and diagnosis of TCM constitutions is nine(Balanced Constitution, Qi-deficient Constitution, Yang-deficient Constitution, Yin-deficient Constitution, Phlegm-dampness Constitution, Damp-heat Constitution, Stagnant blood Constitution, Stagnant qi Constitution, Inherited specific Constitution). And Chinese government established the Classification and Diagnosis Standards for the Constitutions of TCM in 2009. TCM Constitution Theory is being widely applied at Health Administration Center that treats 'predisease' patients. Theses Chinese clinical implications of constitutional medicine are a good example for "Sasang Constituional Medicine" Care Service.

• Journal of Nutrition and Health
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• v.29 no.1
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• pp.70-76
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• 1996

This study was undertaken to investigate the effect on the Cd accumulation in long-term Cd poisoned rats. 40 male weaning Sprague Dawley rats weighting 80-90g were divided into 4 groups (LCuLFeCd : low Cu, Fe and Cd group, ACuLFeCd : adequate Cu, low Fe and Cd group, ACuAFeCd : adequate Cu, adequate Fe and Cd group) according to Cu and Fe levels (Cu 0.5ppm, 8.5ppm : Fe 6ppm, 40ppm) for 12 weeks. There were no significant difference in water intake, feed intake, and body weight gain according to dietary Cu and Fe consumption. But the mean food intake and body weight gain of adequate Fe groups(LCuAFeCd, ACuAFeCd) were higher than those of deficient Fe groups (ACuLFeCd, LCuFeCd)in long-term Cd poisoned rats. The mean Cd levels of serum, liver, kidney, and urine in ACuAFeCd group were lower than those of Cu and /or Fe deficient groups. But the mean fecal Cd excretion of ACuAFeCd group was higher than that of Cu and/or Fe deficient groups. And the mean Cd retention amount of ACuAFeCd group was lower than those of Cu and/or Fe deficinet groups. In conclusion, these results provide an evidence that adequate Cu and Fe intakes can decrease Cd accumulation in rats. Therefore, in the point of increasing environmental Cd contamination, adequate Cu and Fe intakes must be suggested to prevent Cd accumulations.

• The Journal of the Society of Korean Medicine Diagnostics
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• v.18 no.3
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• pp.175-186
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• 2014

The purpose of this study is to give priority in Yin-deficient questionnaire items. if adequate priority is given, clinical judgment and questionnaire of the results is expected to be similar. The AHP is a systematic procedure for analyzing the elements of any problem hierarchically. Based on survey of expertise, as series of pairwise comparison judgements is performed to evaluate the various elements in the hierarchy. We are expected to use the AHP analysis that would apply to oriental doctor's diagnostic process analysis. In this study, korean medical doctors experienced over 5 years was included. Results given the importance to Yin-deficient questionnaire items, Results given the importance to the questionnaire items, the most important item was Irritable fever on the five Hearts, the second was tidal fever.

• BMB Reports
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• v.53 no.9
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• pp.472-477
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• 2020

Osteoclasts are hematopoietic-derived cells that resorb bone. They are required to maintain proper bone homeostasis and skeletal strength. Although osteoclast differentiation depends on receptor activator of NF-κB ligand (RANKL) stimulation, additional molecules further contribute to osteoclast maturation. Here, we demonstrate that protocadherin-7 (Pcdh7) regulates formation of multinucleated osteoclasts and contributes to maintenance of bone homeostasis. We found that Pcdh7 expression is induced by RANKL stimulation, and that RNAi-mediated knockdown of Pcdh7 resulted in impaired formation of osteoclasts. We generated Pcdh7-deficient mice and found increased bone mass due to decreased bone resorption but without any defect in bone formation. Using an in vitro culture system, it was revealed that formation of multinucleated osteoclasts is impaired in Pcdh7-deficient cultures, while no apparent defects were observed in differentiation and function of Pcdh7-deficient osteoblasts. Taken together, these results reveal an osteoclast cell-intrinsic role for Pcdh7 in maintaining bone homeostasis.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.10
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• pp.1348-1354
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• 2002

We investigate the flame behavior and spatial distribution of OH, PAHs and soot in a confined buoyant diffusion flame with decrease of the coflowing air flow rate. Direct photographs and Schlieren images represent that flame is Ally occupied by blue flame and becomes unstable, which is partially detached to the fuel nozzle tip in a near extinction flame under extremely reduced oxidizer condition. Laser induced fluorescence profiles clearly shows that OH is still generated in near-extinction flame, although intensity becomes weak with decreasing air flow rate. But soot scattering image cannot be seen any more in an oxidizer deficient ambience and simultaneously the PAHs are widely distributed downstream. These results are due to that a decrease of oxygen concentration in the combustion chamber leads to a temperature drop of flame, as a consequence, to a delay in soot growth and to a expanding of the PAHs, as soot precursors.

• Toxicological Research
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• v.17
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• pp.289-292
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• 2001

Gene targeting allows precise, predetermined changes to be made in a chosen gene in the mouse genome. To date, targeting has been used most often for generation of animals completely lacking the product of a gene of interest. Models of essential hypertension have been produced by mutated genes relating renin angiotensin system. The most significant contribution to understanding the genetic etiology of essential hypertension is probably the demonstration that discrete alterations in the expression of a variety of different genes can individually cause changes in the blood pressures of mice, even when the mice have all their compensatory mechanisms intact. These effects are readily detected in animals having moderate decreases in gene function due to heterozygosity for gene disruptions or modest increases due to gene duplication. As a species the mouse is highly resistant to atherosclerosis. However. through induced mutations it has been possible to develop lines oj mice that are deficient in apolipoprotein E, a ligand important in lipoprotein clearance, develop atherosclerotic lesions resembling those observed in humans. The atherosclerotic lesions in apoE-deficient mice have been well characterized, and they resemble human lesions in their sites of predilection and progression to the fibroproliferative stage. Other promising models are mice that are deficient in the low-density lipoprotein receptor. Considerable work still remains to be done in dissecting out in a rigorous manner the effects of alterations in single genes on the induction or progression of atherosclerosis and on the control of blood pressures. Perhaps even more exciting is the opportunity now becoming available to breed animals in which the effects oj precise differences in more than one gene can be studied in combination.

• Journal of Nutrition and Health
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• v.27 no.9
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• pp.949-956
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• 1994

The effect of zinc deficiency on certain immunological parameters was investigated using intragastric tube feeding to obviate decreased food intake and altered eating pattern. Male, Fischer 344 rats were bilaterally adrenalectomized(ADX) or sham operated(SHAM). ADX rats received 0.9% NaCl in their drinking water and corticosterone injections at the dose of 1mg/kg of body weight three times per day. After recovery, one half of ADX and SHAM animals were tube-fed a purified, liquid diet containing either two ppm of zinc(zinc-deficient, force-fed ; ZDF) or 50ppm(zinc-replete, force-fed ; ZRF) for 19 days. They received identical amounts of diet based on the intake of ad libitum-fed, zinc-replete rats. Although they received identical amounts of food, ZDF rats grew at a slower rate compared to ZRF rats in both SHAM and ADX rats. Regardless of surgery, force-feeding rats the zinc-deficient diet resulted in a substantial decrease in serum zinc levels. The weights of the thymus, lymph node, and spleen were lower in SHAM-ZRF rats compared to SHAM-ARF rats. Marginal zinc deficiency caused lymphopenia in SHAM animals. However, these differences in lymphoid tissues and cells between SHAM-ZDF and SHAM-ZRF rats disappeared in ADX rats. These results indicate that the impaired growth of lymphoid tissues observed in zinc-deficient, sham-operated animals can be attributed to elevated serum corticosterone levels under the conditions of our experiments.

• Steel and Composite Structures
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• v.29 no.2
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• pp.187-200
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• 2018

This paper handles the repairing of deficient square Concrete-Filled Steel-Tube (CFST) beams subject to bending through an experimental and numerical program. Eight square-CFST beams were tested. A 5-mm artificial notch was induced at mid-span of seven beams, four of them were repaired by using CFRP sheets and two were repaired by using GFRP sheets. The beam deflection, strain and ultimate moments were recorded. It was found that providing different cut-off points for the different layers of FRP sheets prohibited failure at termination points due to stress concentrations. Using different lengths of FRP sheets around the notch retarded crack propagation and prevented FRP rupture at the crack position. Finite element analysis was then conducted and the proposed FE model was verified against the recorded experimental data. The influence of various parameters as FRP sheet length, tensile modulus and the number of layers were studied. The moment capacity of damaged square-CFST beams was improved up to 77.6% when repaired by using four layers of CFRP, however, this caused a dramatic decrease in beam deflection. U-wrapping of notched-CFST beam with 0.75 of its length provided a comparable behaviour as wrapping the full length of the beam.