• International Journal of Stem Cells
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• v.16 no.1
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• pp.93-107
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• 2023

Background and Objectives: Chronic periodontitis can lead to alveolar bone resorption and eventually tooth loss. Stem cells from exfoliated deciduous teeth (SHED) are appropriate bone regeneration seed cells. To track the survival, migration, and differentiation of the transplanted SHED, we used super paramagnetic iron oxide particles (SPIO) Molday ION Rhodamine-B (MIRB) to label and monitor the transplanted cells while repairing periodontal bone defects. Methods and Results: We determined an appropriate dose of MIRB for labeling SHED by examining the growth and osteogenic differentiation of labeled SHED. Finally, SHED was labeled with 25 ㎍ Fe/ml MIRB before being transplanted into rats. Magnetic resonance imaging was used to track SHED survival and migration in vivo due to a low-intensity signal artifact caused by MIRB. HE and immunohistochemical analyses revealed that both MIRB-labeled and unlabeled SHED could promote periodontal bone regeneration. The colocalization of hNUC and MIRB demonstrated that SHED transplanted into rats could survive in vivo. Furthermore, some MIRB-positive cells expressed the osteoblast and osteocyte markers OCN and DMP1, respectively. Enzyme-linked immunosorbent assay revealed that SHED could secrete protein factors, such as IGF-1, OCN, ALP, IL-4, VEGF, and bFGF, which promote bone regeneration. Immunofluorescence staining revealed that the transplanted SHED was surrounded by a large number of host-derived Runx2- and Col II-positive cells that played important roles in the bone healing process. Conclusions: SHED could promote periodontal bone regeneration in rats, and the survival of SHED could be tracked in vivo by labeling them with MIRB. SHED are likely to promote bone healing through both direct differentiation and paracrine mechanisms.

• Journal of the Korean Geotechnical Society
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• v.40 no.4
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• pp.179-188
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• 2024

Nondestructive pile integrity tests are used to confirm the construction of drilled shafts as the foundation of many facilities. However, the safety of the foundation is determined only by the presence or absence of defects, and the location and scale of defects are not considered. In this study, we propose an analysis model for the lateral bearing capacity and section force connected building information modeling (BIM) by extracting the cross-sectional characteristics of the defect in piles and reviewing the safety of piles with defects. Defects at the top of piles had more effect on the change in the deflection of the pile head. Moreover, the decrease in the axial force-bending moment interaction diagram due to cross-sectional reduction increased the risk of destruction of the piles more than the change in the bending moments due to defects. The proposed method can help review the comprehensive safety of piles.

• Journal of Veterinary Clinics
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• v.28 no.5
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• pp.486-496
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• 2011

A special mesenchymal tissue layer called perichondrium has a chondrogenic capacity and is a candidate tissue for engineering of cartilage. To overcome limited potential for chondrocyte proliferation and re-absorption, we studied a method of cartilage tissue engineering comprising chondrocyte-hydrogel pluronic complex (CPC) and cultured perichondrial cell sheet (cPCs) which entirely cover CPC. For effective cartilage regeneration, cell-sheet engineering technique of high-density culture was used for fabrication of cPCs. Hydrogel pluronic as a biomimetic cell carrier used for stable and maintains the chondrocytes. The human cPCs was cultured as a single layer and entirely covered CPC. The tissue engineered constructs were implanted into the dorsal subcutaneous tissue pocket on nude mice (n = 6). CPC without cPCs were used as a controls (N = 6). Engineered cartilage specimens were harvested at 12 weeks after implantation and evaluated with gross morphology and histological examination. Biological analysis was also performed for glycosaminoglycan (GAG) and type II collagen. Indeed, we performed additional in vivo studies of cartilage regeneration using canine large fullthickness chondrial defect model. The dogs were allocated to the experimental groups as treated chondrocyte sheets with perichondrial cell sheet group (n = 4), and chondrocyte sheets only group (n = 4). The histological and biochemical studies performed 12 weeks later as same manners as nude mouse but additional immunofluorescence study. Grossly, the size of cartilage specimen of cPCs covered group was larger than that of the control. On histological examination, the specimen of cPCs covered group showed typical characteristics of cartilage tissue. The contents of GAG and type II collagen were higher in cPCs covered group than that of the control. These studies demonstrated the potential of such CPC/cPCs constructs to support chondrogenesis in vivo. In conclusion, the method of cartilage tissue engineering using cPCs supposed to be an effective method with higher cartilage tissue gain. We suggest a new method of cartilage tissue engineering using cultured perichondrial cell sheet as a promising strategy for cartilage tissue reconstruction.

• International Journal of Naval Architecture and Ocean Engineering
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• v.2 no.1
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• pp.24-33
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• 2010

Design of a Pump Jet Propulsor (PJP) was undertaken for an underwater body with axisymmetric configuration using axial/low compressor design techniques supported by Computational Fluid Dynamics (CFD) analysis for performance prediction. Experimental evaluation of the PJP was earned out through experiments in a Wind Tunnel Facility (WTF) using momentum defect principle for propulsive performance prior to proceeding with extensive experimental evaluation in towing tank and cavitation tunnel. Experiments were particularly conducted with respect to Self Propulsion Point (SPP), residual torque and thrust characteristics over a range of vehicle advance ratio in order to ascertain whether sufficient thrust is developed at the design condition with least possible imbalance torque left out due to residual swirl in the slip stream. Pumpjet and body models were developed for the propulsion tests using Aluminum alloy forged material. Tests were conducted from 0 m/s to 30 m/s at four rotational speeds of the PJP. SPP was determined confirming the thrust development capability of PJP. Estimation of residual torque was carried out at SPP corresponding to speeds of 15, 20 and 25 m/s to examine the effectiveness of the stator. Estimation of thrust and residual torque was also carried out at wind speeds 0 and 6 m/s for PJP RPMs corresponding to self propulsion tests to study the propulsion characteristics during the launch of the vehicle m water where advance ratios are close to Zero. These results are essential to assess the thrust performance at very low advance ratios to accelerate the body and to control the body during initial stages. This technique has turned out to be very useful and economical method for quick assessment of overall performance of the propulsor and generation of exhaustive fluid dynamic data to validate CFD techniques employed.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.44 no.12
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• pp.12-17
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• 2007

The effects of the evaporation rate of MgO films using an electron beam on the MgO properties and the discharge characteristics of a plasma display panel(PDP) were investigated and analyzed. Mgo films were deposited with the various MgO evaporation rates. The MgO properties such as the crystal orientation, the surface roughness, and the film structure, were inspected using XRD(X-ray diffractometry), AFM(atomic force microscopy). From the experiments and Paschen law, the maximum value of the secondary, electron emission coefficient $(\gamma)$ was obtained at the evaporation rate of $5\AA/sec$. The minimum firing voltage and the maximum luminous efficiency were obtained at an evaporation rate of $5\AA/sec$. In the MgO film deposited at $5\AA/sec$, the (200) orientation and $F^+$ center were most intensive. The XRD results and cathode-luminescence(CL) spectra show the $\gamma$ values are correlated with $F/F^+$ centers of the molecular structure of MgO films.

• Applied Chemistry for Engineering
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• v.27 no.4
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• pp.380-385
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• 2016

Nanocelluloses, mainly cellulose nanofibrils (CNF) and cellulose nanocrystals (CNC, i.e., defect-free, rod-like crystalline residues after acid hydrolysis of fibers), have been the subject of recent interest. Due to the presence of hydroxyl groups on the surface of nanocelluloses, their surfaces are reactive, making them suitable candidates for reinforcing materials for manufacturing polymer composites. In this study, CNF was used as a reinforcing material for manufacturing cement composites. CNF was prepared by TEMPO (2,2,6,6,-tetramethyl piperidine-1-oxyl radical) oxidation procedure combined with extensive homogenization and ultrasonication. Transmission electron microscopy (TEM) analysis of the suspension showed the width of CNF between 10 and 15 nm. The compressive strength of cement composites containing 0.5% CNF was comparable to that of conventional cement composites. On the other hand, the tensile and flexural strength were improved by 49.7% and 38.8%, respectively, compared to those of conventional cement composites. Also, at an ambient condition, the degree of self-shrinkage reduction reached to 18.9% in one day, followed by 5.9% in 28 days after molding.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.146-146
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• 2017

현대의 스마트폰 및 태블릿pc등을 가능하게 만든 집적 기술 중의 하나는 3차원 집적 회로(3D-IC)와 같은 패키징 기술이다. 이러한 첨단 3차원 집적 기술은 메모리집적을 통한 대용량 메모리 모듈 개발뿐만 아니라, 메모리와 프로세서의 집적, high-end FPGA, Back side imaging (BSI) 센서 모듈, MEMS 센서와 ASIC 집적, High Bright (HB) LED 모듈 등에 적용되고 있다. 3D-IC의 3차원 모듈 제작 시에는 기존에 발생하지 않았던 여러 가지 파괴 모드들이 발생하고 있는데 Thermal/Photonic Emission 장비 등 기존의 2차원 결함분리 (Fault Isolation) 기술로는 첨단의 3차원 적층 제품들에서 발생하는 불량을 비파괴적으로 혹은 3차원적으로 분리하는 것이 불가능하므로, 비파괴 3차원 결함 분리 기술은 향후 선행 제품 적기 개발에 매우 필수적인 기술이다. 본 연구는 3D-IC 반도체의 비파괴적 내부결함 검사를 위하여 가시광선-근적외선 대역(351nm~1770nm)의 InGaAs (Indium Galium Arsenide) 계열 영상검출기 (imaging detector)를 사용하여 분광 시스템 광학 설계를 통한 초분광 영상 기반 검출 모듈을 제작하였다. 제작된 초분광 영상 기반 검출 모듈을 이용하여 구리 회로 위에 실리콘 웨이퍼가 3단 적층 된 반도체 더미 샘플의 초분광 영상을 촬영하였으며, 촬영된 초분광 영상에 대하여 Chemometrics model 기반의 분석기술을 적용하여 실리콘 웨이퍼 내부의 집적 구조에 대한 검사가 가능함을 확인하였다.

• Journal of the Korean Society for Nondestructive Testing
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• v.23 no.6
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• pp.566-576
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• 2003

Carbon-fiber reinforced plastics (CFRP) composite laminates often possess strong in-plane elastic anisotropy attributable to the fiber orientation and layup sequence. The layup orientation thus greatly influences its properties in a composite laminate. It could result in the part being rejected or discarded if the layup orientation of a ply is misaligned. A nondestructive technique would be very beneficial, which could be used to test the part after curing and to require less time than the optical test. In this paper, ultrasonic scanners were set out for different measurement modalities for acquiring ultrasonic signals as a function of in-plane azimuthal angle. The motorized scanner was built first for making transmission measurements using a pair of normal-incidence shear wave transducers. Another scanner was then built fer the acousto-ultrasonic configuration using contact transducers. A ply-by-ply vector decomposition model has been developed, simplified, and implemented for composite laminates fabricated from unidirectional plies. We have compared the test results with model data. It is found that strong agreement are shown between tests and the model developed in characterizing cured layups of the laminates.

• Journal of Korean Society for Quality Management
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• v.46 no.3
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• pp.483-496
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• 2018

Purpose: This paper proposes a quality strategy based on the evolution of technology in the age of the 4th Industrial Revolution. Methods: We examine the theory of past quality activities and the changes in quality paradigm, and analyze key words for the technologies and key issues of the 4th Industrial Revolution. Based on existing quality management, we find a quality strategy that should be pursued during the 4th Industrial Revolution. Results: Quality has been recognized as an essential component of corporate competitiveness. The paradigm of quality has also changed with the pass of time and industry development. From this viewpoint, the following eight quality strategies are proposed for the development of the technology of the 4th Industrial Revolution period, such as Market-to-customer fusion quality, symbiotic quality, big data quality, technical accuracy and zero-defect quality, facility predictability quality, software quality, process flexibility quality, and information protection stability and security quality. Conclusion: Quality for customer satisfaction is still important nowadays. However, in the 4th Industrial Revolution era, where various business models and methods of manufacturing are expected, the big data utilization, software quality, and the reliability and security of information protection to support it are important.

• Journal of the Korean Society for Precision Engineering
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• v.17 no.10
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• pp.155-161
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• 2000

Recently, environmental pollution has become a big problem in industry and many researches have been done in order to preserve the environment. In the grinding process, the coolant has great influence on environment. It contains several chemicals(sulfur, phosphorus and chlorine) to improve the grinding efficiency. If these additives go into the workplace atmosphere, it is harmful for workers. It can also cause the environment pollution. Because of these reasons many studies have been done to minimize the amount of coolant. However the small amount of coolant can cause the thermal defect on the ground surface layer. This study forced the effects of the compressed cold air when the spindle shaft materials(SCM4 & SCM21) were cylindrical ground with WA wheel. The compressed cold air was used as the coolant and grinding performance was compared with that of the conventional grinding fluids(emulsion). Many experiments were carried out with these two cooling materials. The surface roughness, residual stress, and roundness were measured for the cylindrical grinding. The test results showed that the compressed cold air was very useful as the cooling materials for grinding process. It was also efficient to minimize the thermal defects of workpiece and could also play a role in solving environmental pollution.