• The korean journal of orthodontics
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• v.11 no.2
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• pp.57-64
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• 1981

The purpose of this study was to investigate the effect of hydrocortisone in the fusion mechanism of the secondary palatine shelves in the rat. Pregnant rats were injected with 5.0 mg/100 gm body weight of hydrocortisone for 3 days between the 7th day and the 9th day of pregnancy. Between the 14th day md the 18 day of pregnancy, the fetuses were removed and decapitated to be immersed in $10\%$ formalin and Carney's solution. Preparations were stained with alizarin red S, hematoxylin-eosin and alcian blue respectively, and partly were treated for Periodic acid-Schiff reaction. The results were as followings: 1. The incomplete fusion of the palatal epithelium took place though the polarity of epithelial cells. 2. At the edge of shelves the differentiation of mesenchymal cells was observed, but the inter penetration of mesenchyme was not shown. 3. It was considered that the phenomenon of hypocalcification in matrix had relation to the decrease stainability to alizarin red S in shelves. 4. It might be concluded that the connective tissue under epithelium showed the decrease tendency to alcian blue and PAS reaction due to the inhibition of the synthesis of the mucopolysaccharide.

• The Journal of Internal Korean Medicine
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• v.1 no.1
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• pp.98-106
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• 1976

In order to study the effect of croton oil on the mucous cell in the mice intestin the experimental animals were injected with 0.1gm body weight of croton oil through intraperitoneally. They were sacrificed by ether anesthesia and obtained from distal small intestine and duodenum and colon, and fixed in 10% meutral formal. After embedded in paraffin, sectioned in 5 micro thickness, and stained with P.A.S (Periodic Acid Schiffis) reaction. The average number of the mucous cell was counted in each specimen over 20 fields under 450 magnification. The following results were obtained; 1) An average number of mucous cell began rapidly increase from 15 min and reached high average number after injection of croton oil of mucous cell from 30-60 min after injection. 2) An average number of mucous cell rapidly increase after injection of croton oil and were not reduced normal level by time lapsed 48 hrs. 3) The mucous cell showed with tape of time after injection of croton oil. A type and B type decrease and showed recovery C type decrease and recovery. 4) According to the above findings, it is presumed that croton oil accelerate secretion of mucin of the mucouse cell and production of mucin in growing mucous cell.

• The Journal of Internal Korean Medicine
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• v.27 no.1
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• pp.16-26
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• 2006

Objectives : This experiment was performed to determine the effects of the Jeseubsungi-tang(JSSGT) on hyperlipidemia-induced by hypercholesterolemic diet in mice. Methods : C57BL/6 mice were sensitized with a hypercholesterolemic diet for 6 weeks. Experimental group was treated with $150mg/d{\ell}$ concentrations of JSSGT for 4 weeks (once a day). Results: JSSGT-treated mice showed a body weight decrease compared to normal group. JSSGT-treated mice showed a significant decrease in cholesterol, total cholesterol, triglyceride, ALT and LDL-cholesterol levels compared to hyperlipidemia-induced control group. However, HDL-cholesterol levels in JSSGT-treated group significantly increased compared to the hyperlipidemia-induced control group. Conclusion : These results suggest that JSSGT is effective in treating hyperlipidemia. Because only hyperlipidemic mice were studied, further research is needed.

• Journal of Nutrition and Health
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• v.26 no.3
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• pp.277-285
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• 1993

To investigate the effects of dietary calcium levels on the Ca metabolism in a rat model of ovariectomized osteoporosis, two studies were conducted. In Expt. I, five week-old femalc rats ovariectomized and fed a low Ca diet(0.06% Ca) for four weeks were compared with rats fed a normal (0.53% Ca) or low Ca diet under the sham-operated condition. Ovariectomized rats showed a significant increase in body weight and food intake. In rats fed the low Ca diet, a remarkable decrease was shown regardless of ovariectomy in serum Ca concentration, breaking force of bones, Ca and phopsphrus contents of bones, and apparent absorption and retention of Ca. Furthermore hte decrease of Ca contents of serum and bones in rats ovariectomized and fed the low Ca diet was similar to that in rats model of postmenopausal osteoporosis. In Expt. II, rats ovariectomized and fed on the low Ca diet for four weeks were divided into three groups, those given low Ca diet, normal Ca diet and high Ca diet(1.06%) respectively. The results indicated that supplementations of Ca at the intake level of 0.53% and 1.06% for 4 weeks tend to improve the relative Ca deficiency shown in experimental rat model of ovariectomized osteoporosis.

• Journal of Nutrition and Health
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• v.6 no.1
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• pp.31-38
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• 1973

The effect of dietary condition on the acethylcholine and chotinesterase activity in the brain was observed. Rats weighting $120{\sim}130\;gm$ was fed on diets deprived of protein, carbohydrate and lipid, respectively. Experimental terms was devided into 3, 7 and 15 days. The results are summarized as follows: 1. The lipid free diet makes greater contribution to the gain of body weight than the carbohydrate free and protein free diet. 2. The acethylcholine in the protein free diet group is reduced after 7 days and that in the carbohydrate free diet, after 3 days. 3. The cholinesterase activity shows continuing decrease after 3 days by protein free diet, and the other diet groups appear temporal decrease at 7 days and increase, at 15 days. 4. The relation of acethylcholine, and cholinesterase is similar to each other except that of the carbohydrate diet. From the above results, the conclusions are obtained that the protein free diet makes the acethylcholine and cholinesterase activity lower, and the carbohydrate free diet, acethylcholine lower.

• Advances in Traditional Medicine
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• v.5 no.2
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• pp.160-166
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• 2005

The present study was carried out to investigate the hypoglycemic effect of a polyherbal aqueous extract (Curcuma longa Linn., Emblica officinalis Gaertn., Trigonella foenum-graecum Linn., Enicostemma littorale Blume) in alloxan-induced diabetic rats. Short term experiments showed a decrease in blood glucose levels at $2^{nd}\;hr$ of administration of the aqueous extract in alloxan-induced diabetic rats with increase in serum insulin levels. The extract did not show any effect on blood glucose or serum insulin levels in normoglycaemic rats. Treatment with the extract (1.5 g dry plant equivalent extarct/100 g body weight/day) for 20 days in diabetic rats showed a significant decrease in blood glucose and glycosylated haemoglobin levels and an increase in serum insulin levels. The aqueous extract also showed an enhanced glucose-induced insulin release at 11.1 mM glucose from isolated rat pancreatic islets. The extract did not show any toxicity at the particular dose used.

• Physical Therapy Korea
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• v.6 no.2
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• pp.43-55
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• 1999

In today's society, many women wear high-heeled shoes, but the effect of heel height on lumbar lordosis has not been clearly defined. The objective of this study was to identify the influence of heel height and general characteristics of subjects on lumbar lordosis. The subjects of this study were 40 healthy women who were students of the Department of Physical Therapy, College of Rehabilitation Science, Taegu University. Flexible ruler measurement was used to measure the lumbar lordosis at barefoot, 3 cm and 7 cm high-heeled standing positions. The results were as follows: 1) Significant statistical decrease in lumbar lordosis was observed as heel heights were increased from barefoot to 7 cm high heel. 2) There were no statistically significant differences between lumbar lordosis according to three different heel heights and weight, body mass index. 3) Lumbar lordosis measured at different heel heights was related to subject's height. With increasing subject's height, lumbar lordosis that measured from each heel height was significantly decreased. As heel heights were increased from barefoot to 7 cm high heel, significant statistical decrease in lumbar lordosis was observed in the subjects whose height were 151~160 cm. 4) Intrarater reliability on lumbar lordosis taken with a flexible ruler was good, with Cronbach ${\alpha}$ values of 0.8971 for barefoot, 0.8107 for 3 cm and 0.9002 for 7 cm high-heeled standing positions.

• Applied Microscopy
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• v.50
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• pp.29.1-29.6
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• 2020

Erythronium japonicum (E. japonicum) and Corylopsis coreana Uyeki (C. coreana Uyeki, Korean winter hazel) have been shown to significantly decrease 1,3-dichloro-2-propanol (1,3-DCP)-induced generation of reactive oxygen species and CYP2E1 activity in HuH7, human hepatocytes. In this study, we expanded upon the previous study and investigated the effects of E. japonicum and C. coreana Uyeki extracts on 1,3-DCP-induced liver damage in rats. The pre-treatment of rats with these extracts alleviated a decrease in body weight and reduced 1,3-DCP-induced increase in catalytic activities of hepatic enzymes, such as aspartate aminotransferase and alanine aminotransferase, in the serum. Moreover, treatment with the extracts restored the 1,3-DCP-induced decreases in anti-oxidant enzyme activities, such as the activities of superoxide dismutase and catalase, in the rat liver. Histopathological studies also strongly supported the results of enzyme activities. These results suggest a possibility that the extracts of E. japonicum and C. coreana Uyeki can be a remedy for alleviating 1,3-DCP-induced liver damage in animals.

• Toxicological Research
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• v.12 no.2
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• pp.309-318
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• 1996

This study was conducted to investigate the subacute intravenous toxicity of rHu-EPO in Sprague-Dawley rats. rHu-EPO was daily administered to male and female rats for 30 days with different dose levels(100, 500, 2,500 unit/ kg). After the administration period of 30 days, 5 males and 5females rats per each dose group were assigned for recovery period of additional 30 days. There were no clinical signs compared with control group but slight decrease in spontaneous motor activities and locomotions were observed at high dose groups of males and females. In males, feed consumption was reduced at 500 unit/kg and body weight gain was retarded. In the administration sites of tail, focal congestion was observed in a few animals treated with rHu-EPO. No deaths were occurred during the course of study. In hematological examination. a significant increase of hemoglobin and hematocrit was observed in the males and females rats receiving high dose of rHu-EPO. rHu-EPO administration for 30 days showed a marked decrease in glucose concentration. At the highest dose groups, there was a significant incerase in the weights of spleens in both sexes. but this was considered to be related to its pharmacological activity. These results indicate that rHu-EPO does not induce any significant toxic effect on Sprague-Dawley rats for 30 days.

• Korean Journal of Acupuncture
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• v.36 no.4
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• pp.221-229
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• 2019

Objectives : This study investigated the hepatoprotective effect of herbal mixture including Lycii fructus (HML) in thioacetamide (TAA)-induced hepatotoxicity in mice. Methods : To confirm the liver protective effect, induced by TAA for 3 days injection at 100 mg/kg mice, HML were treated for 8 weeks at 300 mg/kg/day, 1000 mg/kg/day. Positive control was treated silymarin 50 mg/kg/day after TAA injection. The changes of mortality rate, clinical signs, organ weight, relative liver, blood chemistry and histopathological findings were analyzed after experiment. Results : Body weight gain was observed in all groups, but TAA treated group at 4th week and all treated groups decreased weight compared to the untreated group. As a result of organ weight measurement, organ weight gain due to hepatic injury was observed statistically significantly in TAA-treated group and TAA+Silymarin treated group, and the herbal mixture-treated group showed a tendency to decrease compared to the TAA treated group. Blood biochemistry showed that total cholesterol and very low density lipoprotein cholesterol decreased statistically in TAA+low-dose and high dose herbal mixture treated group compared to the TAA-treated group. Histopathological examination showed that liver abnormalities were not observed in untreated group, liver fibrosis was observed in liver injury with TAA treated and herbal mixture treated group. And, TAA+high dose herbal mixture group showed relaxation tendency on liver calcification compared to the TAA treated group. Conclusions : According to the above results, HML provided hepatoprotective effects on the hepatic injury by reduction of inflammatory responses.