• 한국가축번식학회지
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• 제25권2호
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• pp.139-145
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• 2001

본 연구는 Pb중독이 rat의 생리현상에 미치는 영향을 구명 하고자 연속적 인 Pb acetate 투여가 사료와 물섭취량, 증체량, 혈액치 및 장기내 무기물 수준의 변동을 측정하였다. 1. Pb acetate 1,000, 2,000 및 4,000 ppm/kg을 rat에 투여했을 때 사료섭취량은 정상대조군에 비해 점차 감소하는 경향을 나타냈고 용량이 증가할수록 사료섭 취 량은 유의 한 감소를 나타냈다. 2. Pb acetate 1,000, 2,000 및 4,000 ppm/kg을 rat에 투여했을 때 음수량은 정상대조군에 비해 점차 감소하는 경향을 나타냈고 Pb 용량이 증가할수록 음수량은 감소하였다. 3. Pb acetate 1,000, 2,000 및 4,000 ppm/kg을 rat에 투여했을 때 증체량은 정상대조군에 비해 약간 감소된 수준을 나타냈으나 Pb용량이 증가할수록 증체량은 점차 감소하였다. 4. Pb acetate 1,000, 2,000 및 4,000 ppm/kg을 rat에 투여했을 때 혈액치중 RBC, WBC 및 PLT치는 정상대조군에 비해 유의한 증가를 나타냈고, Hb량과 PCV치는 정상대조군에 비해 약간 감소를 나타냈다. 5. Pb acetate 2,000 및 4,000 ppm/kg을 rat에 투여했을 때 간내 Pb, Mn, Fe, Pb 및 Zn의 잔류함량은 정상대조군에 유의한 증가를 나타냈다. Pb 2,000 및 4,000 ppm/kg을 rat에 투여했을 때 신장내 Pb, Mn, Fe, Pb, Zn의 함량은 정상대주군에 비해 증가를 나타냈다.

• Toxicological Research
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• 제26권1호
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• pp.67-74
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• 2010

The present study was conducted to obtain information on the effects of amitraz on reproductive and developmental parameters in rats. The test chemical was administered via the drinking water containing 0, 40, 120, and 360 ppm to male rats from 2 weeks before mating to the end of 14-day mating period and to females from 2 weeks before mating, throughout mating, gestation and up to lactational day 4. During the study period, clinical signs, body weights, food intake, organ weights, reproductive and littering findings, necropsy findings, sperm parameters, and histopathology were examined. At 360 ppm, decreases in the body weight gain, food consumption, and the number of live pups and an increase in the post-implantation loss were observed. In addition, decreases in the seminal vesicle weight and sperm motility were found in males. At 120 ppm, a decrease in the food consumption was found transiently in both males and females, but no reproductive and developmental toxicity was observed in both sexes. There were no signs of either general or reproductive and developmental toxicity in the 40 ppm group. Based on these results, it was concluded that the repeated oral administration of amitraz to rats resulted in a decrease in the food consumption at 120 ppm and decreases in the seminal vesicle weight, sperm motility, and the number of live pups and an increase in the post-implantation loss at 360 ppm in rats. Under these experimental conditions, the no-observed-adverse-effect level (NOAEL) of amitraz for general and reproduction/developmental toxicity was believed to be 120 ppm, and the no-observed-effect level (NOEL) of amitraz was believed to be 40 ppm in rats.

• Journal of Nutrition and Health
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• 제37권9호
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• pp.786-793
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• 2004

Women with menopause or rats with ovariectomy is associated with increased body weight, body fat and insulin resistance, which are components of metabolic syndrome. Increased prevalence of metabolic syndrome after menopause might be associated with mitochondrial dysfunction, since mitochondrial oxidative and phosphorylation activity is strongly correlated with insulin sensitivity. Although estradiol replacement prevents the metabolic syndrome, harmful effect of estradiol hampers the casual usage to prevent the metabolic syndrome. It has been reported that genistein has a mild estrogenic activity, decreases fat mass in mice and has an antidiabetic role in diabetic rats. Although insulin resistance is closely related to mitochondrial functions, there has not been yet any study in regard to the effect of dietary genistein on mitochondrial function in the insulin resistant female subjects induced by ovariectomy or similar situation. The present study investigated whether the supplementation of genistein in the high fat diet affected the mitochondrial function of high fat fed ovariectomized rats. Female Sprague Dawley rats (8 weeks old) were assigned to the following groups: sham-operated+ high fat diet (S, n=6); sham-operated + high fat diet with 0.1% genistein (S + G, n=7); ovariectomized + high fat diet (OVX, n=8); ovariectomized + high fat diet with 0.1% genistein (OVX+ G, n=8). Ovariectomy significantly increased body weight compared with S group. Genistein consumption in ovariectomized (OVX + G) rats decreased body weight gain compared with OVX rats. Liver weights were increased by ovariectomy. The hepatic mitochondrial protein density expressed as mg per g liver was lower in the OVX group than in the S group. However, OVX + G group showed the increased mitochondrial protein density similar to the level of S group. When mRNA levels of genes related to mitochondria such as peroxisome proliferator-activated receptor ${\gamma}$ coactivator 1 (PGC-1) and cytochrome c oxidase subunit III (COX III) were measured, there were decreases in the mRNA levels of PGC-1 and COX III in S + G, OVX and OVX + G group. The activity of cytochrome c oxidase was not different between groups. We could observe the decrease in succinate dehydrogenase (SDH) activity per g liver in OVX rats. Genistein supplement increased SDH activity. In conclusion, genistein supplementation to the OVX rats enhanced mitochondrial function by increasing mitochondrial protein density and SDH activity. The improvement in mitochondrial function by genistein can contribute to the improvement in metabolic syndrome.

• 대한미생물학회지
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• 제13권1호
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• pp.55-62
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• 1978

항암제(抗癌劑)로 잘 알려진 cyclophosphamide(CY)를 성숙(成熟)한 마우스에 체중(體重) kg당(當) 300mg을 복강내(腹腔內)에 투여(投與)하여 CY가 체중(體重), 비장중량(脾臟重量) 및 말초임파조직(末梢淋巴組織)에 미치는 영향(影響)을 실험(實驗)하였다. CY는 마우스의 체중(體重)을 약간(若干) 감소(減少)시켰으나 체중(體重)은 곧 회복(回復)되었다. CY가 비장중량(脾臟重量)에 미치는 영향(影響)은 현저(顯著)하였는데 초기(初期)엔 비장중량(脾臟重量)은 감소(減少)되었으나 곧 회복(回復)되고 그 후(後) 정상(正常)보다 더 증가(增加)되었는데 그 증가(增加)는 CY투여(投與) 20일(日)이 지나서야 정상(正常)으로 되돌아왔다. 비장(脾臟) 및 임파절(淋巴節)의 조직학적(組織學的) 형태(形態)에 미치는 CY의 영향(影響)은 다양(多樣)하였다. 즉(卽), 흉선의존세포(胸線依存細胞)가 위치(位置)하는 비장(脾臟)의 periarterial lymphatic sheath와 임파절(淋巴節)의 paracortex는 골수유래세포(骨髓由來細胞)가 위치(位置)하는 follicles보다 1일(日) 내지(乃至) 2일(日) 늦게 소실(消失)되었으며 비장(脾臟)의 비대(肥大)가 최고(最高)에 달(達죠)하였을때 비장(脾臟)이나 임파절(淋巴節)의 구조(構造)는 동일(同一)한 임파양세포(淋巴樣細胞)로 구성(構成)된 interstitial tissue로 대치(代置)되어 있었다. 비장(脾臟)의 중량(重量)이 정상화(正常化)되어감에 따라 비장(脾臟)이나 임파절(淋巴節)의 구조(構造)가 정상화(正常化) 되어갔다. 이와 같은 본(本) 실험(實驗)의 소견(所見)은 CY는 골수유래세포(骨髓由來細胞) 뿐만 아니라 흉선의존세포(胸線依存細胞)에도 영향(影響)을 미친다고 사료(思料)된다. 저자(著者)들은 전보(前報)에서 CY에 의(依)한 지연성과민반응(遲延性過敏反應)의 항진(亢進)은 suppressor T cell의 제거(除去)에 기인(基因)한 것이라고 시사(示唆)하였는데 본(本) 실험결과(實驗結果)는 이를 뒷받침해준다고 사료(思料)되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제15권5호
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• pp.682-686
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• 2002

Ten growing male buffalo calves (aged 6-8 months, average body weight $88.2{\pm}0.57$ kg were divided randomly into two groups. Animals in group I were fed on concentrate mixture A (CP 20.2%, TDN 77.4%) and urea- ammoniated wheat straw (UAWS) while the animals in group II were fed on concentrate mixture B (CP 17.9%, TDN 77.6%) and HCl plus urea treated wheat straw (HCl UAWS) to meet their nutrient requirement for 500 g gain/d as per Kearl (1982). This feeding practice lasted for 120 days, during which fortnightly body weight were recorded to assess their growth rate. A metabolism trial was conducted after 90 days of experimental feeding to compare the digestibility of nutrients, their balance, plane of nutrition and relative cost of feeding in two groups of animals. Results revealed a significant increase in the CP content of ammoniated wheat straw due to addition of HCl viz 12.1% from 7.5%. There was a decrease in the intake of DM (p<0.05), OM (p<0.05), EE (p<0.05), NDF (p<0.01), ADF (p<0.01), cellulose (p<0.01) and hemicellulose (p<0.01) in group II as compared to group I. The digestibility (%) of DM, OM and CP was significantly (p<0.01) more in group II, whereas the digestibility (%) was significantly more for NDF (p<0.05) and hemicellulose (p<0.01) in group I than group II. There was no significant difference in the N, Ca and P balance in two groups. Intake of total DM (g/d) or (g/kg $W^{0.75}$) was significantly (p<0.01) more in group I as compared to group II, whereas the intake of DCP and TDN (g/d or g/kg $W^{0.75}$) was alike in two groups. The total body weight gain (kg), average daily gain (g/d) and feed conversion efficiency were significantly (p<0.01) more in UAWS fed group as compared to HCl UAWS fed group. Feeding cost (Rs./kg. weight gain) was significantly (p<0.05) more in group II as compared to group I. It is concluded that HCl UAWS is not suitable for the feeding of growing buffalo calves as it reduced the growth rate in comparison to UAWS fed buffalo calves.

• 한국동물학회지
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• 제7권2호
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• pp.6-12
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• 1964

The present paper deals with the changes in serum protein fraction, total serum protein, hematocrit, red blood cell, haemoglobin, and weight of liver or kidney : body weight ratio of irradiated and non-irradiated snake head, Ophicephalus argus(CANTOR) . Irrardiation doses are 2537$\AA$-7 minutes (7M) and 2537$\AA$-15 minutes (15M). Serum electrophoretic patterns showed a marked decrease in albumin fraction at 1, 3, 9, 12 and 15 days on 7M group and 15 M group. On both experimental groups percentage increases in $\alpha$1 and $\beta$ fractions occurred at different time periods in general but are interpreted as only apparent changes accompanying the greater albumin fall. ${\gamma}$-Globulin decreased at 1, 6 and 15 days on 7M group. and at 1, 3, 6 and 9 days on 15M group. Also, A/G ratio was significantly low in groups subjected to above conditions as compared to the controls. A/G ratio decreased at 3 and 15 days remarkably on both groups. On the average, the reductions in the A/G ratio were not proportional to themagnitude of ultraviolet-light. Total protein of serum changed according to suggestive changes in electrophoretic patterns of serum . Total protein of serum declined at 1, 3, 6, 9 and 12 day periods on 7M groups and at 1, 3, 9, 12 and 15 day-periods on 15 M group, and increased approaching control values at 15 and 18 day-periods on both groups. Hematocrit increased remarkably at 1, 3, 6 and 12 days and decreased at 9 and 15 days on 7M group, and increased throughout the sampling period on 15M . Red blood cell decreased throughout the sampling period and increased slightly at 6 days on 7 M group and decreased at 1, 6, 9, 15 and 18 days and increased remarkably at 3 and 12 days on 15M group. Hemoglobin decreased remarkably at 1 day-period and increased at other days on both groups. The liver weight was not remarkably changed after whole body irradiation on both groups. and kidney was increased from 1st day on 7M group and 3 rd day on 15M group respectively. It appears that changes in electropphoretic patterns of serum, A/G ratio, total protein of serum, hematocrit, red blood cell, hemoglobin, and liver or kidney weight act to the detriment of the animal following non-ionizing irradiation.

• 한방재활의학과학회지
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• 제21권2호
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• pp.265-278
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• 2011

Objectives : This trial were conducted to evaluate the efficacy and adverse events of Bofutsusho-san(BTS) and Boiogiot-tang(BOT) by oriental obesity pattern identification on obese subjects, as compared to placebo. Methods : 166 subjects(body mass index ${\geq}25kg/m^2$) were recruited and randomized to receive BT(n=55), FH(n=55) or placebo(n=56) for 8 weeks. Anthropometric factors, serum lipid, glucose, blood pressure(BP), pulse rate, resting metabolic rate and oriental obesity pattern identification questionnaire were measured at baseline and 8 weeks. Adverse events and safety outcome variables were also checked during trials. Results : The frequency of top-scored oriental obesity pattern was ordered by indigestion(食積) > stagnation of the liver qi(肝鬱) > yang deficiency(陽虛) > spleen deficiency(脾虛) > phlegm(痰飮) > blood stasis(瘀血) in subjects. BTS group significantly decreased body weight, body mass index(BMI), waist circumference(WC), body fat mass, total cholesterol and HDL-cholesterol in stagnation of the liver qi(肝鬱) and WC in indigestion(食積). BOT group showed significant decrease of body weight, BMI, WC, and body fat mass in indigestion(食積) not in deficiency(虛症). Adverse events were reported most frequently in yang deficiency(陽虛) by BT group and stagnation of the liver qi(肝鬱) by BOT group. Conclusions : Bofu-tsusho-san was effective in treating obesity with stagnation of the liver qi(肝鬱). Obesity pattern identification could be a useful diagnostic tool predicting treatment effects and adverse events.

• 한국수산과학회지
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• 제25권5호
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• pp.371-382
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• 1992

자주새우, Crangon affinis 서식지의 동계$(7^{\circ}C$, 춘. 추계$(15^{\circ}C$ 및 하계$(25^{\circ}C$의 평균 수온대에서 일정기간 (0, 5, 11, 17, 25days) 절식시킨 후 체물질의 화학적조성, 산소소비 및 질소배설률을 측정하여 환경변화에 대한 생리적 적응의 일면을 조사하였다. 1. 자주새우의 체물질조성은 단백질 $68.6\%$, 지질 $7.0\%$, 탄수화물$4.1\%$, 키틴$6.3\%$, 그리고 회분은 $14.6\%$였다. 2. 기아상태 에 서도 고온일수록 탈피 빈도는 높았으며 동일 절식기간 동안 고온일수록 체중 및 체물질의 감소는 더욱 컸다. 3. 산소소비율은 기아가 계속됨에 따라 고온일수록 현저히 감소하는 경향을 보였으나 기아 25일 경과 후의 각 수온구별 산소소비율은 유사하였다. 4. 수온별 각 실험구간의 $Q_{10}$치는 실험온도구간에 큰 차이를 보이지 않았으나 기아가 계속됨에 따라 상당히 감소하였다. 5. 총배설질소량은 고온일수록 증가하였으며 배설질소의 주성분은 암모니아였다 그리고 총배설질소량은 어느 실험수온구에서나 기아가 계속됨에 따라 현저하게 증가하였다. 6. 기아 25일 경과후 수온 7, 15 및 $25^{\circ}C$에서 이화된 단백질은 각각 체단백질의 1.8, 2.5 및 $3.3\%$였다. 7. 기아초기의 O:N 원자비는 22-25이고, 기아상태가 지속됨 에 따라 8이하로 낮아져 기아동안의 에너지원으로 단백질을 이용함을 보였다.

• 한국식품영양과학회지
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• 제30권5호
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• pp.906-912
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• 2001

유산소성 운동과 김치보충제 섭취가 비만 여중생의 신체 조성 및 혈중지질에 미치는 영향을 알아보기 위하여 운동군은 HRmax 60~85%운동강도의 조깅, 줄넘기운동을 매회 60분, 주 4회 실시하고, 김치 보충제 섭취군은 500 mg 의 김치 분말이 들어 있는 김치 보충제 6알 총 3g (김치약 30g)을 총 6주간 섬취하게 하였다. 실험에 참가한 여중생은 총 38명으로 운동군(EG) 8명, 김치 보충제 군(KG)12명, 그리고 운동과 김치 보충제 섭취를 병행한 군(EKG) 8명의 비만여중생 28명, 그리고 정상체중을 지닌 대조군(CG) 10명이었다. 운동 또는 김치 보충제를 6주간 섭취한 EKG, EG 그리고 KG는 CG 그룹에 비해 신체 조성 및 혈중 지질 농도가 유의하게 감소하였다. 체중, BMI 체지방 및 체지방율의 감소 정도는 EKG, EG 그리고 KG 순이었으며, 비만도를 낮추는 효과는 EKG, KG 그리고 EG순으로 나타났다. 총 콜레스테롤 및 LDL-콜레스테롤 감소는 EKG, KG 그리고 EG 순으로 나타났으며, 중성지질 감소는 EKG, EG, 그리고 KG 순이었고, HDL-콜레스테롤 농는 EKG와 EG 그룸이 KG와 CG에서 유의하게 증가하였다. 이상의 결과를 살펴보았을 때 혈중 중서이방 농도를 포함하여 체지방 조성과 관련 있는 요인들은 운동이 김치 보충제 섭취보다 효과가 있는 것으로 나타났고 혈중 콜레스테롤 농도를 감소시키는 효과는 운동보다는 김치 보충제 섭취가 효과적이었다. 그러나, 비만 여중생의 비만을 조절하는데 있어서 이 두 방법을 단독으로 실시한 결과보다는 두용법을 병행한 즉, 김치 보충제를 섭취하면서 운동을 병행한 군의 효고가 가장 현저하게 나타났다. 이상의 결과로부터 여중생의 비만을 치료하는데 있어 운동 이외에 김치를 많이 섭취하는 식이요법을 병행하명 상당한 효과가 있을 것으로 관찰되었다.

• Toxicological Research
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• 제12권1호
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• pp.47-52
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• 1996

The renal toxicity of the extract of Polygalae Radix was investigated in rats. Rats were treated with 3.5 mg/Kg of the extract, i.p., for 7 days. Changes in consumatory behavior, 24 hour-urine and the activities of urinary enzymes were determined during the administration of the extract. Significant decrease in body weight and food consumption and increase in 24 hour-urine volume were observed during the administration. However, the quantity of total creatinine in urine was decreased significantly. Those indicate that subacute treatment with the extract might induce diuresis and the ditiresis might be due to the decrease in water reabsorption. In the activities of urinary enzymes, the activities of alanine aminopejotidase (AAP) and gamma-glutamyl transpeptidase (GGT) were increased 4.3 and 3.5 times and then returned to the control. The activity of N-acetyl-${\beta}$-D-glucosaminidase (NAG) was increased 7.2 times and then decreased slowly. But, it was significantly higher than that of the control evea after the last administration. The activity of factate dehydrogenase (LDH) was increased continuozlsly during the treatment. It showed 32 times higher than the control. These results suggested that the extract of Polygalae Radix had toxic effect on kidney. Furthermore, the result suggested that the subacute administration of the extract induced resistance against the toxicity of Polygalae Radix.