• 제목/요약/키워드: decrease in fertility

검색결과 141건 처리시간 0.022초

수컷 생쥐 생식기관의 형태에 미치는 에스트로겐 수용체 알파의 선택적 촉진제의 농도별 영향 (Concentration Effect of Estrogen Receptor-${\alpha}$ Selective Agonist on the Morphology of Reproductive Organs of the Male Mice)

  • 한지연;조영국;조현욱
    • Applied Microscopy
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    • 제41권1호
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    • pp.37-45
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    • 2011
  • 암컷호르몬으로 알려져 있는 에스트로겐이 실제로는 수컷 생식기관에도 영향을 미친다. 에스트로겐은 수용체와 결합하여 그 작용을 나타낸다. Propyl pyrazole triol (PPT)은 에스트로겐 수용체 베타에 비해 상대적으로 알파에 410배 혹은 1,000배 더 높은 선택적 친화성으로 결합하는 에스트로겐 수용체 알파 촉진제이다. 본 연구에서는 성체 수컷 생쥐를 대상으로 castor oil에 각각 0.01 mg, 0.1 mg, 1 mg, 4mg의 농도로 희석한 PPT를 1주에 1회씩 8주 동안 피하주사 하였다. 대조군은 castor oil을 주 1회씩 동일한 기간 동안 생쥐에 피하주사 하였다. 정소, 수출소관, 부정소의 조직학적 변화를 현미경으로 관찰하였다. PPT 4mg의 고농도 투여군에서는 체중, 정소, 부정소의 무게가 뚜렷하게 감소되었다. 실험기간 동안 PPT에 의해 세정관의 직경, 부정소의 상피세포 높이가 감소되었다. 부정소에 붙어 있던 지방세포의 크기는 PPT에 의해 줄어들었다. PPT 4 mg 투여군의 경우, 부정소의 꼬리 부위에 저장되는 정자가 관찰되지 않았다. 결론적으로 고농도의 PPT 투여로 인해 성체 수컷 생쥐의 정자형성 억제와 같은 생리학적 변화가 유발되었고 동시에 생식기관의 조직학적 변화도 유발되었다.

Effects of heat stress on conception in Holstein and Jersey cattle and oocyte maturation in vitro

  • Jihwan Lee;Doosan Kim;Junkyu Son;Donghyeon Kim;Eunjeong Jeon;Dajinsol Jung;Manhye Han;Seungmin Ha;Seongsoo Hwang;Inchul Choi
    • Journal of Animal Science and Technology
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    • 제65권2호
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    • pp.324-335
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    • 2023
  • Korea, located in East Asia in the northern hemisphere, is experiencing severe climate changes. Specifically, the heat stress caused by global warming is negatively affecting the dairy sector, including milk production and reproductive performance, as the major dairy cattle Holstein-Friesian is particularly susceptible to heat stress. Here, we collected artificial insemination and pregnancy data of the Holstein and the Jersey cows from a dairy farm from 2014 to 2021 and analyzed the association between the conception rate and the temperature-humidity index, calculated using the data from the closest official weather station. As the temperature-humidity index threshold increased, the conception rate gradually decreased. However, this decrease was steeper in the Holstein breed than in the Jersey one at a temperature-humidity index threshold of 75. To evaluate the effects of heat stress on the oocyte quality, we examined the nuclear and cytoplasmic maturation of Holstein (n = 158, obtained from six animals) and Jersey oocytes (n = 123, obtained from six animals), obtained by ovum pick-up. There were no differences in the nuclear maturation between the different conditions (heat stress: 40.5℃, non- heat stress: 37.5℃) or breeds, although the Holstein oocytes seemed to have a lower metaphase II development (p = 0.0521) after in vitro maturation under heat stress conditions. However, we found that the Holstein metaphase II oocytes exposed to heat stress presented more reactive oxygen species and a peripheral distribution of the mitochondria, compared to those of the Jersey cattle. Here, we show that weather information from local meteorological stations can be used to calculate the temperature-humidity index threshold at which heat stress influences the conception rate, and that the Jersey cows are more tolerant to heat stress in terms of their conception rate at a temperature-humidity index over 75. The lower fertility of the Holstein cows is likely attributed to impaired cytoplasmic maturation induced by heat stress. Thus, the Jersey cows can be a good breed for the sustainability of dairy farms for addressing climate changes in South Korea, as they are more resistant to hyperthermia.

Effect of Short-term and Long-term Preservation on Motion Characteristics of Garole Ram Spermatozoa: A Prolific Microsheep Breed of India

  • Joshi, Anil;Bag, Sadhan;Naqvi, S.M.K.;Sharma, R.C.;Rawat, P.S.;Mittal, J.P.
    • Asian-Australasian Journal of Animal Sciences
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    • 제14권11호
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    • pp.1527-1533
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    • 2001
  • Garole is a prolific, rare, less known and small size Indian sheep breed found in low and humid Sunderban region of West Bengal. Although information on stored Garole ram liquid semen upto 24 h is available, but there is a need to further investigate the short-term and long-term preservability of Garole ram semen for extensive utilization of this valuable germplasm by artificial insemination. The aim of the present study was to apply computer-assisted sperm analysis technique for assessing the motion characteristics of Garole ram semen stored (i) in liquid state at refrigeration temperature for short-term preservation upto 48 h and (ii) in frozen state at $-196^{\circ}C$ for long-term preservation after packaging in mini straws. Short-term preservation had a significant effect on motility (p<0.01) as the motility progressively decreased from 90.1% at 0 h to 85.5% and 73.2% after 24 and 48 h of storage, respectively. Although the decline in rapid moving sperms was also significant (p<0.01) on storage but the decrease was more pronounced at 48 h as compared to 24 h of storage period. Storage of chilled semen had also a significant effect on % linearity (p<0.05), % straightness (p<0.01), sperm velocities (p<0.01), amplitude of lateral head displacement (p<0.01) and beat frequency (pO.Ol) of spermatozoa. The replication had a significant effect for all the variables except average path and straight line velocity. However, the interactions of short-term storage and replication were non-significant for most of the variables except % of medium moving sperms, sperm velocities and beat frequency. On long-term preservation of Garole ram spermatozoa under controlled conditions the mean post-thaw recovery of 70.4 and 71.4% motile spermatozoa was achieved having 48.8 and 48.9% of rapidly motile spermatozoa, respectively in both the replicates. The effect of replication on cryopreservation was significant (p<0.05) on amplitude of lateral head displacement and beat frequency, but there was no significant effect on motility, rapidly motile spermatozoa, linearity, straightness and sperm velocities of frozen-thawed spermatozoa. It can be concluded from these results that an average 70% motility can be achieved on storage of Garole ram semen in chilled liquid state upto 48 h or in liquid nitrogen after freezing under controlled conditions in straws. However, further studies are required to evaluate the fertility of short-term and long-term preserved Garole ram semen for extensive use of this prolific sheep breed.

벼 품종들의 출수기 이동에 따른 광합성 및 온도와 등숙과의 관계 (Relationship among Photosynthesis, Grain Filling and Temperature of Rice Cultivars by Shifted of Heading Date)

  • 이석영
    • 한국작물학회지
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    • 제40권3호
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    • pp.398-405
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    • 1995
  • 벼 자포니카 품종과 통일형 품종에 있어서 출수기가 달라질 경우 포장 조건에서 광합성 능력과 등숙량과의 관계 및 적당한 등숙온도 범위를 알기 위해 시험한 결과를 요약하면 다음과 같다. 1. 동화산물 수용기관의 크기를 결정하는 요인 중의 하나인 임실율은 출수기의 이동에 따른 차이를 보이지 않아 수량에 영향를 미치지 않았다. 2. 4가지 벼품종의 등숙양상은 8월 16일, 26일 출수할 경우 대체로 sigmoid 곡선을 보였으나 출수기가 9월 5일로 늦어질 경우 초기의 등숙속도가 빨라져 log곡선에 가까운 형태로 변하였다. 3. 자포니카에서는 생리적 성숙기가 9월 5일 출수한 경우 8월 16일, 또는 26일 출수한 것에 비해 40일로 길어졌는데 출수 30일 이후의 낮 평균온도가 17$^{\circ}C$ 정도이고 밤 평균온도가 12$^{\circ}C$ 정도이며 최저온도가 3~8$^{\circ}C$라도 10일 정도는 계속해서 등숙을 하였다. 4. 통일형의 경우 낮평균 온도가 2$0^{\circ}C$이고 밤평균 온도가 17$^{\circ}C$이며 최저기온이 1$0^{\circ}C$ 이상일 경우 소량이나마 등숙이 가능하나 최저온도가 1$0^{\circ}C$ 미만인 경우 등숙이 거의 불가능하였다. 5. 벼 잎의 광합성 능력과 포장의 온도, 상부 3엽의 면적, 일장의 적으로 나타낸 광합성양 계산치는 출수기가 달라짐에 따라 많은 차이를 보였고, 통일형의 경우 최저온도가 4$^{\circ}C$ 미만으로 떨어질 경우 광합성 능력이 0에 가까웠으며 온도가 올라가도 광합성능력이 회복되지 않았다. 6. 자포니카와 통일형 모두에 있어서 다른 조건이 좋은 경우 등숙에 적당한 온도범위는 21~26$^{\circ}C$로 나타났다.

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SATEEC 시스템을 이용한 객토 토양의 토성고려에 따른 도암댐 유역의 토양유실 및 유사량 분석 (Analysis of Soil Erosion and Sediment Yields at the Doam-dam Watershed considering Soil Properties from the Soil Reconditioned Agricultural Fields using SATEEC System)

  • 유동선;안재훈;윤정숙;허성구;박윤식;김종건;임경재;김기성
    • 한국물환경학회지
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    • 제23권4호
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    • pp.518-526
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    • 2007
  • There have been serious soil erosion and water pollution problems caused by highland agriculture practices at Doam-dam watershed. Especially agricultural activities, chemical and organic fertilizer and pesticide applications, soil reconditioning to maintain soil fertility are known as primary causes of soil erosion and water qaulity degradation in the receiving water bodies. Among these, soil reconditioning can accelerate soil erosion rates. To develop soil erosion prevention practices, it is necessary to estimate the soil erosion from the watershed. Thus, the Universal Soil Loss Equation (USLE) model has been developed and utilized to assess soil erosion. However, the USLE model cannot be used at watershed scale because it does not consider sediment delivery ratio (SDR) for watershed application. For this reason, the Sediment Assessment Tool for Effective Erosion Control (SA TEEC) was developed to assess the sediment yield at any point in the watershed. The USLE-based SA TEEC system can estimate the SDR using area-based SDR and slope-based SDR module. In this study, the SATEEC system was used to estimate soil erosion and sediment yield at the Doam-dam watershed using the soil properties from reconditioned agricultural fields. Based on the soil sampling and analysis, the US LE K factor was calculated and used in the SA TEEC system to analyze the possible errors of previous USLE application studies using soil properties from the digital soil map, and compared with that using soil properties obtained in this study. The estimated soil erosion at the Doam-dam watershed without using soil properties obtained in the soil sampling and analysis is 1,791,400 ton/year (123 ton/ha/year), while the soil erosion amount is 2,429,900 ton/year (166.8 ton/ha/year) with the use of soil properties from the soil sampling and analysis. There is 35 % increase in estimated soil erosion and sediment yield with the use of soil properties from soil reconditioned agricultural fields. Since significant amount of soil erosion are known to be occurring from the agricultural fields, the soil erosion and sediment yield from only agricultural fields was assessed. The soil erosion rate is 45.9 ton/ha/year without considering soil properties from soil reconditioned agricultural fields, while 105.3 ton/ha/year after considering soil properties obtained in this study, increased in 129%. This study shows that it is very important to use correct soil properties to assess soil erosion and sediment yield simulation. It is recommended that further studies are needed to develop environment friendly soil reconditioning method should be developed and implemented to decrease the speed of soil erosion rates and water quality degradation.

암컷 마우스 생식기관의 형태에 미치는 에스트로겐 수용체 촉진제의 영향 (Effects of Estrogen Receptor Agonist on Morphology in the Female Mouse Reproductive Organs)

  • 이은정;한지연;조현욱
    • Applied Microscopy
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    • 제39권4호
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    • pp.301-309
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    • 2009
  • 에스트로겐은 수컷과 암컷의 생식기관 내에서 구조적이고 기능적인 변화를 유발하지만 그 정확한 기작에 대하여는 잘 알려져 있지 않다. 에스트로겐의 기능과 연관지어, 본 연구에서는 암컷 생식기관 내에서 에스트로겐 수용체 촉진제인 4,4',4"-(4-propyl-[1H]-pyrazole-1,3,5-triyl)tris phenol (PPT)의 작용을 확인하고자 하였다. 성체 암컷 마우스에 castor oil 0.06 mL에 PPT 3mg 농도로 희석한 용액을 1주일에 1회씩 3주, 5주, 8주 동안 각각 피하주사 하였으며, 대조군에는 castor oil 0.06 mL만을 투여하였다. 광학현미경을 이용하여 생식기관에 대한 PPT의 영향을 분석하였다. PPT에 의해 실험기간 동안 체중, 난소 및 지방조직의 무게가 감소되었다. PPT 투여군에서는 대조군에 비해 난소의 직경이 감소되었으며, 난소 내 그라프여포와 황체의 수도 감소되었다. 자궁의 경우, PPT에 의해 근육층과 내막층의 높이가 감소함에 따라 내강이 증가되었다. 또한 자궁샘의 수도 감소되었다. 이런 결과들로 볼 때 PPT에 의해 암컷 생식기관의 형태학적 변화가 유발되고 이로 인해 생식력에도 변화가 있을 것으로 보인다.

Low-dose Radiation Induces Antitumor Effects and Erythrocyte System Hormesis

  • Yu, Hong-Sheng;Liu, Zi-Min;Yu, Xiao-Yun;Song, Ai-Qin;Liu, Ning;Wang, Hao
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권7호
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    • pp.4121-4126
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    • 2013
  • Objective: Low dose radiation may stimulate the growth and development of animals, increase life span, enhance fertility, and downgrade the incidence of tumor occurrence.The aim of this study was to investigate the antitumor effect and hormesis in an erythrocyte system induced by low-dose radiation. Methods: Kunming strain male mice were subcutaneously implanted with S180 sarcoma cells in the right inguen as an experimental in situ animal model. Six hours before implantation, the mice were given 75mGy whole body X-ray radiation. Tumor growth was observed 5 days later, and the tumor volume was calculated every other day. Fifteen days later, all mice were killed to measure the tumor weight, and to observe necrotic areas and tumor-infiltration-lymphoreticular cells (TILs). At the same time, erythrocyte immune function and the level of 2,3-diphosphoglyceric acid (2,3-DPG) were determined. Immunohistochemical staining was used to detect the expression of EPO and VEGFR of tumor tissues. Results: The mice pre-exposed to low dose radiation had a lower tumor formation rate than those without low dose radiation (P < 0.05). The tumor growth slowed down significantly in mice pre-exposed to low dose radiation; the average tumor weight in mice pre-exposed to low dose radiation was lighter too (P < 0.05). The tumor necrosis areas were larger and TILs were more in the radiation group than those of the group without radiation. The erythrocyte immune function, the level of 2,3-DPG in the low dose radiation group were higher than those of the group without radiation (P < 0.05). After irradiation the expression of EPO of tumor tissues in LDR group decreased with time. LDR-24h, LDR-48h and LDR-72h groups were all statistically significantly different from sham-irradiation group. The expression of VEGFR also decreased, and LDR-24h group was the lowest (P < 0.05). Conclusion: Low dose radiation could markedly increase the anti-tumor ability of the organism and improve the erythrocyte immune function and the ability of carrying $O_2$. Low-dose total body irradiation, within a certain period of time, can decrease the expression of hypoxia factor EPO and VEGFR, which may improve the situation of tumor hypoxia and radiosensitivity of tumor itself.

지역별 가계지출 부담이 기혼여성의 출산 의사에 미치는 영향: 수도권과 비수도권 비교를 중심으로 (Comparing the Effects of Regional Household Expenditure Burden on Childbirth Intention of Married Women: The Case of Capital and Non-Capital Regions)

  • 이다은;서원석
    • 지적과 국토정보
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    • 제51권2호
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    • pp.151-168
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    • 2021
  • 본 연구는 수도권과 비수도권의 가계지출 부담이 기혼여성의 출산 의사에 미치는 영향을 패널로짓 모형을 이용해 비교분석하였다. 실증분석의 핵심 대상인 기혼여성의 범위는 가임 가능성이 높은 25세부터 39세까지로 한정하였다. 주요 분석 결과는 다음과 같다. 첫째, 거주 지역과는 관계없이 대체로 배우자의 경제력이 기혼여성의 출산 의사에 있어 중요한 요인일 수 있다는 점을 확인하였다. 둘째, 결혼생활 만족도는 높을수록 출산 의사에 긍정적인 영향을 미치고 있었으며, 자녀가 반드시 있어야 한다는 가치관이 있을수록 출산 의사가 높아지는 것으로 나타났다. 셋째, 가계지출 부담은 기존 자녀 수와 같은 내부요인을 제외하고 기혼여성의 출산 의사에 있어 가장 중요한 영향을 미치는 요인임을 확인하였다. 특히 수도권과 비수도권 모두 교육비 지출에 대한 부담은 출산 의사를 큰 폭으로 감소시키는 핵심 원인으로 나타났다. 넷째, 가계지출 부담은 거주 지역에 따라 출산 의사에 상이한 영향을 보이는 것으로 나타났는데, 수도권은 의료비 및 원리금 대출이, 비수도권은 교통통신비가 출산 의사에 더 큰 영향을 미치고 있었다. 이를 통해 본 연구는 출산 제고를 위해서는 가계지출에 대한 부담완화가 지속적으로 필요하며, 거주 지역에 따라 차별적인 정책접근이 요구된다는 시사점을 확인하였다.

컴퓨터 정액자동분석에 의한 동결융해 한우 정액의 운동특성 연구 (Motional kinematics of Frozen-thawed Korean native cattle semen use of computer aided semen analysis(CASA) system)

  • 이강남;이병천;김정태;박종임;신태영;황우석
    • 대한수의학회지
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    • 제38권4호
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    • pp.898-908
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    • 1998
  • The aim of this experiments were to assess the time-interval change of motional characteristics in frozen-thawed semen of Korean native cattle (KNC) by using computer aided semen analysis (CASA) technology. Twenty-six KNC frozen semen straws were obtained from Korean KNC improvement department, livestock improvement main division, national livestock cooperatives federation in Korea. Specimens were allowed to thaw at $37^{\circ}C$ for 30 sec in water bath. Semen analysis was performed on semen image analysis system (SIAS, Medical supply, Korea) adjusted to the gate settings and used the semen droplet ($5{\mu}l$) placed on Makler counting chamber (Sefi medical instrument, Israel) prewarmed at $37^{\circ}C$. The same person used the same micropipette to fill the Makler counting chamber. A total of 150 or more of sperms were analysed in each specimen by a single trained person by scanning at least 5 to 10 fields. The measurement parameters in SIAS were as follows ; frame rate = 30 frames per sec, image capture = 1 sec, minimum motile speed = $10{\mu}m/s$, maximum countable sperm number = 400. Statistical analysis was done by Student t-test with use of the Sigma plot program on a IBM personal computer. The dancemean(DNM) and hyperactivated sperm(HYP) of frozen-thawed KNC semen kinematics were significantly decreased(p < 0.05) after 10 min of incubation at $37^{\circ}C$ water bath. But, wobble(WOB) of same sample semen was significantly increased(p < 0.05) after 10 min of incubation and significantly decrease(p < 0.05) after 60 min of same incubation. And, after 30 mim of incubation, significantly differences were found most of motion kinematics, motifity(MOT), curvilinear velocity(VCL), straight line velocity(VSL), average path velocity(VAP), amplitude of lateral head displacement(ALH), beat cross frequency(BCF), mean angular displacement(MAD), dance(DNC), on same sample semen. The DNM of KNC semen sample was variable kinematics after 30 min of incubation. Also, the linearity(LIN) and straightness(STR) was significantly decreased(p < 0.05) from 60 min of incubation. In conclusion, the AI within 30 min after thawing of frozen semen can be an effective method for obtaining high fertility rate in KNC reproductive program.

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지역거점 공공병원의 분만부 공간구성에 관한 연구(1) (A Study on the Space Composition for Department of Delivery in Regional Public Hospital)

  • 박경현;신화경;채철균
    • 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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    • 제28권3호
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    • pp.47-54
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    • 2022
  • Purpose: This study presents the analysis on space usage of delivery departments in regional public hospitals. The results intention is to achieve improvement of the delivery environment for the mothers and newborns regarding exposure prevention and efficient infection control. The purpose of this study is to provide fundamental data for architectural plans and guidelines for the delivery department. Method: The investigation and analysis were based on research papers, legal systems, public medical statistical data, and the architectural floor plan drawing. For research, 20 regional public hospitals with an operating delivery room were excluded. Regarding data accessibility, 15 regional public hospitals were selected. Results: To overcome the increased vulnerability of the delivery department, the research results of basic data is provided for the establishment to address urgent needs and rapid response. Thus, the research results are as follows: Firstly, the delivery department needs to respond promptly according to the type of patients. For example, in a case of emergency surgery, a connected circulation plan with the related departments is needed. Secondly, for the environment of the delivery area, alleviating anxiety is imperative for pregnant patients and guardians, labor, childbirth, and recovery. Therefore, these needs must be addressed for treatment space and circulation. Lastly, the delivery department is classified into three areas for analysis: access area, treatment area, and support area. In most of the delivery departments of the 15 selected hospitals, there is no space for the access and support area except for the labor and delivery rooms in the treatment area. For the access area, a waiting area, changing room for pregnant women and guardians, and a storage space for contaminated linens are required for infection prevention, safety, and efficiency. For the treatment area, childbirth processes and circulation should have space reserved for labor, delivery, recovery, examination, and treatment. In preparation for an emergency during childbirth, emergency response measures and supporting space needs to be established. For the support area, circulation and rooms are to be designed for medical staff support, activity space, storage and transportation of equipment, and urgent medical treatment. Implications: Along with the low fertility rate and the decrease of medical institutions that operate delivery departments, for the purpose of establishing a public medical service system and a healthy medical environment for mothers and newborns, the researched information demonstrates basic data on space plan of delivery departments in regional public hospitals.