• Journal of Microbiology
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• v.41 no.4
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• pp.300-305
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• 2003

Intracellular expression of RNA decoys, such as TAR or RRE decoy, has been previously shown to protect immune cells from human immunodeficiency virus type 1 (HIV-1) replication by inhibiting the binding of the HIV-1 regulatory protein to the authentic HIV RNA sequence. However, HIV-1 challenge experiments of primary human T cells, which express the RNA decoy, demonstrated that the cells were only transiently protected, and hence, more improved protocols for HIV-1 inhibition with the RNA decoys need to be developed. In this report, in order to develop a more effective RNA decoy, we analyzed and compared the ability of a series of RNA decoy derivatives in inhibiting HIV-1 replication in CEM cells. Using an improved tRNA cassette to express high levels of RNA decoy transcripts in cells, we found that co-expression of both TAR and RRE decoys, in the form of an aligned sequence in a single transcription cassette, much more potently blocked cells from HIV-1 than the expression of only one kind of RNA decoy. This observation will have an important implication for experiments involving optimization of clinical applications in RNA decoy-based gene therapy against HIV-1.

• Journal of the Korea Institute of Military Science and Technology
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• v.27 no.3
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• pp.416-427
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• 2024

The wake-homing underwater guided weapon that detects and tracks wake generated during voyage of a surface ship is impossible to avoid with the present acoustic deception torpedo defense system. Therefore, research on bubble-generating wake decoy is necessary to deceive wake-homing underwater guided weapon. Experiments in various environments are required to verify the effective operation method and performance of the wake decoy, but performance verification through underwater experiment is limited. In this paper, we develop a simulator for an torpedo defense system of surface ship, which is applied bubble-generating wake decoy, against acoustic, wake, and hybrid homing underwater guided weapon attack. The simulator includes surface ship model, acoustic decoy(static, mobile) model, bubble-generating wake decoy model, search and motion model of underwater guided weapon and so on. By integrating various models, MATLAB GUI simulator was developed. Through the simulation results for various environmental variables by this simulator, it is judged that effective operation method and performance verification of the bubble-generating wake decoy can be performed.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.10
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• pp.2646-2650
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• 2009

This paper describes an anti-ARM technique using multiple decoy antennas. We investigate the radar and decoy antenna signal received at the ARM receiver to verify the effect of decoy antennas. And we develop a simulation program using Matlab for optimum positions of the decoys and the effect of ARM by distance between decoys. We suggest optimum positions and distance between decoys based on our simulation results.

• Journal of Ocean Engineering and Technology
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• v.35 no.3
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• pp.238-246
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• 2021

The survivability of a naval ship is the ability of the ship and its onboard systems to remain functional and continue a designated mission in man-made hostile environments. A passive decoy system is primarily used as a weapon system for improving the survivability of a naval ship. In this study, an engagement scenario-based simulation program was developed for decoy effectiveness assessments against an anti-ship missile (ASM), which tracks a target with sea-skimming and active radar homing. The program can explain the characteristics of a target ship, such as the radar cross section and evasive maneuvers, as well as the operational performance of the onboard decoy system, the guidance method of the ASM, and the engagement environment's wind speed and direction. This paper describes the theory and formulations, configuration, and user interface of the developed program. Numerical examples of a decoy effect assessment of a virtual naval ship against an ASM are presented.

• KIISE Transactions on Computing Practices
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• v.22 no.1
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• pp.56-60
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• 2016

Peptide identification in tandem mass spectrometry is usually done by searching the spectra against target databases consisting of reference protein sequences. To control false discovery rates for high-confidence peptide identification, spectra are also searched against decoy databases constructed by permuting reference protein sequences. In this case, a peptide of the same sequence could be included in both the target and the decoy databases or multiple entries of a same peptide could exist in the decoy database. These phenomena make the protein identification problem complicated. Thus, it is important to minimize the number of such redundant peptides for accurate protein identification. In this regard, we examined two popular methods for decoy database generation: 'pseudo-shuffling' and 'pseudo-reversing'. We experimented with target databases of varying sizes and investigated the effect of the maximum number of missed cleavage sites allowed in a peptide (MC), which is one of the parameters for target and decoy database generation. In our experiments, the level of redundancy in decoy databases was proportional to the target database size and the value of MC, due to the increase in the number of short peptides (7 to 10 AA). Moreover, 'pseudo-reversing' always generated decoy databases with lower levels of redundancy compared to 'pseudo-shuffling'.

• Journal of the Korean Institute of Intelligent Systems
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• v.25 no.5
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• pp.495-501
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• 2015

Abroad warships are confronted with various menaces. The most critical threat of the warship is an Anti-Ship Missile (ASM). The ASM is able to be launched at a variety of environments and platforms. The ASM can evades conventional naval radar systems and electronic countermeasure techniques for providing a fatal damage to the warship. To cope with the ASM, an active decoy is an effective method to minimize the direct damage to the warship. The active decoy increases survivability of the warship because the ASM can lure pursuit of the active decoy instead of the warship. In this paper, our proposed method verifies an available response time of the active decoy to deal with the ASM using the active decoy of the warship in marine environments. We defined models of the warship, the ASM, and the active decoy, and executed simulation by combining the models. By the simulation result, the proposed method demonstrated the superiority of the mobile active decoy of the response time decoy among various active decoys, and estimated a protection area to prevent the ASM according the response time of the mobile active decoy against the ASM.

• Korean Journal of Microbiology
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• v.39 no.4
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• pp.235-241
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• 2003

We have previously demonstrated that intracellular expression of an RNA aptamer termed RRE40, which was selected in vitro to bind HIV Rev 10-fold much tighter than wild-type RRE, efficiently protected human CD4+ T cell line, CEM, from HIV-1. In this study, to evaluate the efficacy of the RRE40 RNA in clinical settings, polyclonal CD4+ peripheral blood lymphocytes (PBLs) were transduced with retroviral vectors expressing RRE40 decoy RNA and then challenged with clinical isolates of HIV-1. In contrast to the control cells transduced with vectors expressing control tRNA, intracellular expression of RRE40 RNA more effectively inhibited HIV-1 replication in CD4+ PBLs. However, transient and diminished inhibition, rather than complete inhibition, of HIV-1 replication in PBLs expressing RRE40 decoys have been observed. These results suggest that RRE40 decoy RNA would be useful to inhibit HIV-1 replication in cells. However, development of more efficient gene transfer protocols and/or more effective decoy RNAs would be needed to apply RNA decoy to modulate HIV-1 patient.

• IMMUNE NETWORK
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• v.16 no.2
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• pp.134-139
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• 2016

Programmed death-1 (PD-1) is a strong negative regulator of T lymphocytes in tumor-microenvironment. By engaging PD-1 ligand (PD-L1) on tumor cells, PD-1 on T cell surface inhibits anti-tumor reactivity of tumor-infiltrating T cells. Systemic blockade of PD-1 function using blocking antibodies has shown significant therapeutic efficacy in clinical trials. However, approximately 10 to 15% of treated patients exhibited serious autoimmune responses due to the activation of self-reactive lymphocytes. To achieve selective activation of tumor-specific T cells, we generated T cells expressing a dominant-negative deletion mutant of PD-1 (PD-1 decoy) via retroviral transduction. PD-1 decoy increased IFN-γ secretion of antigen-specific T cells in response to tumor cells expressing the cognate antigen. Adoptive transfer of PD-1 decoy-expressing T cells into tumor-bearing mice potentiated T cell-mediated tumor regression. Thus, T cell-specific blockade of PD-1 could be a useful strategy for enhancing both efficacy and safety of anti-tumor T cell therapy.

• KSBB Journal
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• v.19 no.5
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• pp.335-340
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• 2004

Mesangial expansion caused by cell proliferation and glomerular extracellular matrix accumulation is one of the earliest renal abnormalties observed at the onset of hyperglycemia in diabetes mellitus. Transcription factor Sp1 is implicated in the transcriptional regulation of a wide range of genes participating in cell proliferation, and is assumed to play an essential role in mesangial expansion, transforming growth factor (TGF)-$\beta$1, plasminogen activator inhibitor (PAI)-1. We have generated a phosphorothioated double-stranded Sp1-decoy oligodeoxynucleotide that effectively blocks Sp1 binding to the promoter region for transcriptional regulation of TGF-$\beta$1 and PAI-1. The Sp1 decoy oligodeoxynucleotide suppressed transcription of these cytokines and proliferation of primary rat mesangial cells in response to serum stimulation. These results suggest that the Sp1 decoy oligodeoxynucleotide could bea powerful tool in preventing the pathogenesis of renal hypertrophy.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.2
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• pp.491-496
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• 2010

This paper describes an analysis of the effectiveness for an anti-ARM technique to protect a radar system. We investigate the optimum deployment of decoy antennas which are widely using for anti-ARM and the electromagnetic field at the ARM receiver. To verify the effect of decoy antennas, we analyze the field intensity and phase at the receiver and develop a numerical simulation program using Matlab. We conduct an analysis of ARM attack in case of using multiple decoy antennas and these results can be used to decide the optimum positions of the decoys for anti-ARM.