• Applied Biological Chemistry
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• v.48 no.3
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• pp.258-262
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• 2005

For the purpose of developing natural antioxidant, the antioxidative activity and antimicrobial of phenolics isolated from Baek-bu-ja (Aconiti koreani Rhizoma) were determined. Optimum extracting condition for phenolics were water extracts. HPLC analysis showed that the four major phenolic metabolites were rosemarinic, protocatechuic, caffeic and chlorogenic acids. The water extracts of Baekbuja did not have antimicrobial activity against H. pylori; however, the ethanol extracts revealed higher antimicrobial activity. Electron donation ability on DPPH of Baekbuja ethanol extract was 20% higher than other ethanol extracts. The 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid radical decolorization (ABTS) and antioxidant protection factor (PF) were determined with extracts from Aconiti koreani Rhizoma. 94% inhibition and 1.14 PF were shown on ABTS and antioxidant protection factor with 60% ethanol extracts. Also, TBARS (thiobarbituric acid reactive substances) showed $0.19\;{\mu}M$ in the control and $0.07\;{\mu}M$ in the 80% ethanol extracts. The result suggests that Baekbuja extract may be useful as potential sources of anti Helicobacter pylori, antioxidant.

• Korean Journal of Microbiology
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• v.9 no.4
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• pp.163-168
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• 1971

Neutral protease which was obtained from a genus of Aspergilli as the crystal form were investigated for their purification and properties. The results of biochemical and enzymatic studies for their purification and properties in this enzyme were as follows. 1) On the wheat media containing 70%-water and $CaCo_{3}$, Aspergilus oryzae S.H.W. 131 is satisfactorily grown under the basic optimum conditions temperature $27^{\circ}C$- $30^{\circ}C$at relative humidity 100% for three days. 2) The enzyme solution extracted with water is successively purified through the passing on column of Asmti-177N for decolorization of it. And ion exchanger such as DEAAE Sphadex A-50 or Shepadex G-100 and fraction collector is necessary for the sepearte treatments of this enzyme. After washing it with organic solvents as aceton-EtOH, etc., it should be dried on the vacuum dryer at $40^{\circ}C$) The protease activity is determined by the amounts of amino acids, tyrosine. 4) The optimum pH of neutral protease is 6.0-8.0. 5) In effectively decomposing with this neutral protease, the optimum temperature is $35^{\circ}C$. 6) It is interesting that the amounts of metal ion affects the activity of neutral protease. For examples, if it were treated with manganic ion, its activity would be more effective than any other that.

• Food Science and Preservation
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• v.11 no.3
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• pp.383-387
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• 2004

For the purpose of developing natural antioxidant, the antioxidative activity of phenolics isolated from Korean Mulberry(Morus) fruits was determined. Optimum extracting condition for phenolics were 60$\%$(v/v) ethanol. The content of rutin and isoquercetrin as flavonoids were 82.83 mg/100 g and 43.23 mg/100 g respectively. The 2,2'-azinobis-3-ethyl benzothiazoline-6-sulfonic acid radical decolorization and antioxidant protection factor (PF) were determined with extracts from Mulberry fruits. Results showed 98$\%$ inhibition on ABTs by 80$\%$ ethanol extract and 1.25 PF on antioxidant protection factor by 60$\%$ ethanol extract. Electron donation ability on DPPH was higher 60$\%$ ethanol extract than other ethanol extracts. Also, hydroxyl radical scavenging activity of extracts was higher 60$\%$ ethanol extract than other extracts.

• Korean Journal of Plant Resources
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• v.22 no.4
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• pp.343-348
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• 2009

The aim of the study was to assess the cosmeceutical activity of Kalopanax septemlobus leaf and it is possible that can be used as a cosmetic ingredient for applicaiton of cosmetic industries. The concentration of total phenolic compound of hot water and 70% EtOH extracts of K. septemlobus leaf showed 104 mg/L and 125 mg/L respectively. In the result of DPPH(1,1- diphenyl-2-picryl -hydrazyl) scavenging radical activity, 70% EtOH extracts of K. septemlobus leaf showed 93.1% and it was similar to BHA(butylated hydroxyanisole) effect at 1,000ppm concentration. Xanthine oxidase inhibition of hot water extracts and 70% EtOH extracts of K. septemlobus leaf were 46.6% and 60.4% at 1,000ppm, respectively. In these results, K. septemlobus leaf has a great potential as a cosmeceutical ingredient with a natural anti-oxidant source.

• Applied Biological Chemistry
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• v.49 no.4
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• pp.298-303
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• 2006

The biological and antioxidative activity of Phellinus linteus extracts from gradient ethanol concentrations were examined. The phenol contents of Phellinus linteus(28.36 mg/100 ml) was higher in the 80% ethanol extracts than other extracts. Electron donation ability on DPPH of 80% and 90% ethanol extracts(94.12% and 94.14% inhibition) from Phellinus linteus were the highest. The antioxidant activity against water soluble materials of Phellinus linteus ethanol extracts showed totally high inhibition rates above 80%, especially in 80% and 90% ethanol extracts, they showed each 94.12% inhibition and 94.14% inhibition. The inhibition against ABTS [2,2azinobis(3-ethylbenzothiazoline-6-sulfonic acid)] radical decolorization of 80% ethanol extracts was the highest as 96.2%. The antioxidant protection factor (PF) against lipid soluble materials was the highest in 80% ethanol extracts as 1.69 PF, and TBARS of 80% and 90% ethanol extracts were lower as $1.15{\times}100{\mu}M$ and $1.21{\times}100{\mu}M$ than control($1.95{\times}100{\mu}M$. Angiotensin converting enzyme and xanthine oxidase inhibitory activity of 80% ethanol extracts from Phellinus linteus was higher as 95.10%, 85.07% than other extracts. The results to analized of simple phenolic compounds of Phellinus linteus ethanol extrcts with HPLC showed that they were procatecuic acid, caffeic acid and coumaric acid.

• Food Science and Biotechnology
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• v.16 no.5
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• pp.822-827
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• 2007

The aroma characteristics and antioxidative activity of volatile compounds in heat-treated garlic (Allium sativum L.) were evaluated. The garlic was heated to various temperatures (100, 110, 120, and $130^{\circ}C$) for different lengths of time (1, 2, and 3 hr). The volatile compounds of heated garlic were extracted by simultaneous steam distillation extraction (SDE). Aroma compound profiles were analyzed by gas chromatography/mass spectrometry (GC/MS) and antioxidative activity was measured by 2,2-diphenyl-2-picrylhydrazyl (DPPH) assay and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) cation decolorization assay. The major aroma compounds were sulfur compounds such as dimethyl disulfide, 2-propen-1-ol, methyl-2-propenyl disulfide, dimethyl trisulfide, diallyl disulfide, methyl-2-propenyl trisulfide, and di-2-propenyl trisulfide. DPPH radical scavenging activity (EDA, %) and the ascorbic acid equivalent antioxidant activity (AEAC) of volatile compounds in heated garlic increased significantly with the increase of temperature and time (p<0.001). The EDA (%) and AEAC of raw garlic were 26.8%/10 mg garlic and 39.05 mg ascorbic acid equivalent per g sample. After heat treatment, the highest values were 40.50%/10 mg garlic for EDA (%) and 46.43 mg ascorbic acid equivalent per g sample for ABTS.

• Bulletin of the Korean Chemical Society
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• v.31 no.5
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• pp.1275-1279
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• 2010

We have carried out the surface modification of photocatalytic $TiO_2$ with triethoxysilane through dehydrogenation reaction and characterized the modified photocatalyst by spectroscopic methods, such as FT-IR, solid-state $^{29}Si$ MAS NMR, XPS, and XRF, etc. We also examined photocatalytic activity of the immobilized photocatalytic titanium dioxide with triethoxysilane by decolorization reaction of dyes such as cong red and methylene blue under visible light. Dispersion test showed that the photocatalytic titanium dioxide immobilized with triethoxysilane group has kept higher dispersibility than titanium dioxide itself. No appreciable precipitation takes place even after standing for 24 h in the 4:6 mixture ratio of ethanol and water.

• Journal of Environmental Science International
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• v.7 no.4
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• pp.473-480
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• 1998

The bacterial strain JE-1 degrading and utilizing Congo Red as a sole carbon source was isolated from dye-contaminated soul and Identified as Enterobacter species. Enterobacter sp. JE-1 had the highesc decolorization ability when It was cultured In the medium containing 0.05% $NH_4N0_3, 0.05% K_2HP0_4, 0. 03%$ $MgSO_4$, $7H_2O$, 0.025% Congo Red, initial pH 7.0 at $30^{\circ}C$, respectively Enterobacter sp. n-1 had the relatively high substrate specificity. The dye decolorizing activity was exclusively extracellular. The expected metabolic intermediates of Congo Red by Enterobacter sp.15-1 were analyzed by GC/MS. As a result. metabolic products like hauadecanoic acid, 1, 2, 3-triphenylcyclopropene, aliphatic hydrocarbons butylester were detected. Benzldine 616 not detected.

• Journal of the Korean Ceramic Society
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• v.52 no.6
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• pp.489-496
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• 2015

In this study, novel $Ag_2Se$ sensitized $TiO_2$ nanocomposites were prepared by facile sonochemical assisted synthesis method. The as-prepared products were characterized by X-ray diffraction (XRD), Scanning electron microscopy (SEM) with energy dispersive X-ray (EDX) analysis, transmission electron microscopy (TEM), and N2 adsorption BET analysis. The as-prepared $Ag_2Se/TiO_2$ nanocomposites simultaneously possessed great adsorptivity for organic dyes and efficient charge separation properties. In the decolorization of rhodamine B, a significant enhancement in the reaction rate was observed for the $Ag_2Se/TiO_2$ nanocomposite compared to the cases of using pure P25 or $TiO_2$. The sonocatalysis activity was higher due to the greater formation of reactive radicals, as well as to the increase of the active surface area of the $Ag_2Se/TiO_2$ nanocomposite.

• The Journal of the Society of Korean Medicine Diagnostics
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• v.22 no.1
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• pp.11-18
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• 2018

Objectives The incidence of degenerative diseases is increasing as society ages, and this increased the economic burden on society. Oxidative stress was also under suspicion as one of the causes. Recent studies have found that Curcuma longa Rhizoma had antioxidant, anti-inflammatory, antimutagenic, and anticancer effects. Through this study, we figure out trends in Curcuma longa Rhizoma research and evaluate the therapeutic potential of Curcuma longa Rhizoma for dementia. Methods The search was made using domestic and international internet search sites. "Naver academic," "Google scholar," "Korea institute of oriental medicine (KIOM OASIS)," "Korean traditional knowledge portal (KTKP)," "Library of Congress," "Research information sharing service (RISS)," "National Digital Science Links (NDSL)," and "Pub med" are used to search. The searched for words were "turmeric," "Curcuma longa Rhizoma," "antioxidant," and "nerve regeneration." Results It could be seen that a lot of research done on Curcuma longa recently. In previous studies, the most common research disease was cancer. In previous studies, the most common effects of Curcuma longa was antioxidant and anticancer. Curcumin, Vitamin B1, and various essential oils were the functional components of Curcuma longa Rhizoma. Curcuma longa Rhizoma performs DPPH radical scavenging activity and ABTS radical cation decolorization activity; it inhibits NO and iNOS. Conclusion The destruction of nerve cells due to oxidative stress is a cause of dementia. Curcuma longa Rhizoma have antioxidant effects. Therefore, we believed that it is an effective treatment for dementia. Various studies will be made on Curcuma longa Rhizoma.