• Title/Summary/Keyword: decoloring

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Hydrogen Peroxide, Its Measurement and Effect During Enzymatic Decoloring of Congo Red

  • Woo, Sung-Whan;Cho, Jeung-Suk;Hur, Byung-Ki;Shin, Dong-Hoon;Ryu, Keun-Gap;Kim, Eun-Ki
    • Journal of Microbiology and Biotechnology
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    • v.13 no.5
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    • pp.773-777
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    • 2003
  • The color of Congo red hinders the spectrometric measurements of a concentration of hydrogen peroxide and enzyme activity (Horseradish peroxidase; HRP) during enzymatic decoloring of Congo red. In this study, a method was developed to measure peroxidase activity and hydrogen peroxide concentration in the presence of Congo red. The oxidation product of HRP/hydrogen peroxide and ABTS(2,2'-azino-bis-(3-ethylbenzotriazoline-6-sulfonic acid)) formed a dark green color. The spectrum of this product showed absorption bands at 420 nm and 734 nm. When compared with the Congo red spectrum, the absorption at 734 nm of this product did not overlap with Congo red, thus making the hydrogen peroxide measurement possible even in the presence of Congo red. Kinetic study of decoloring of Congo red performed by this method showed that the decoloring reaction followed the Michaelis-Menten kinetics. Pulse feeding of hydrogen peroxide, upon depletion, significantly increased the decoloring of Congo red. This result shows that this newly developed technique can monitor, predict, and improve the enzymatic decoloring process.

Hydrogen peroxide, its measurement and effect during enzymatic decoloring of Congo Red

  • U, Seong-Hwan;Jo, Jeong-Suk;Kim, Gap-Jeong;Kim, Eun-Gi
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.194-197
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    • 2000
  • The color of textile-wastewater hindered spectrometric measurements of $H_2O_2$ and enzyme activity during enzymatic decoloring. By using ABTS, we developed a new method for measuring peroxidase activity and $H_2O_2$ concentration. The ratio of enzyme and $H_2O_2$ was optimized as 1:150 by investigating the effects of $H_2O_2$ on enzymatic decoloring. Pulse feeding of $H_2O_2$, upon depletion, significantly increased the decoloring of Congo Red.

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The Effects of Glass Color Tone by Oxidation Power Index (발화력지수(醱化力指數)가 유리색조(色調)에 미치는 영향(影響))

  • Park, Eun-Seong;Park, Byeong-Gi
    • Journal of Korean Society for Quality Management
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    • v.16 no.1
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    • pp.1-7
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    • 1988
  • The most important element upon the quality of family glass products is the color of glass, which is the first object of consumers concern in purchasing. Therefore, colorless and transparent color tone can level up the additional value of the products. But we have produced the glass products in a large quantity by using the automatic tank brazier, chronic problem has been left over the color tone. By compounding and analizing the management techniques applied by peculiar technique and design of experiments pertinently, and by adjusting the decoloring composition ratio, we can contribute to improvement of color tone as well as to the cost reduction by diminishing the unnecessary decoloring agent. Intangible effect was the securing the superiority of quality home and abroad with the improvement of color tone and was taking a triangular position the factors influencing the glass decoloring which had been an unexhausted field. Tangible effect was the reduction of decoloring cost by diminishing the unnecessary oxidant. We registered the optimal composition ratio condition which was searched by applicating the design of experiments. To maintain good color tone, we manage the degree of purity, the most important thing among the items of color tone, continuously by control chart and it has maintained steady state now.

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Isolation and Characterization of Soil Streptomyces Involved in 2,4-Dichlorophenol Oxidation

  • Kang, Min-Jin;Kang, Ja-Kyoung;Kim, Eung-Soo
    • Journal of Microbiology and Biotechnology
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    • v.9 no.6
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    • pp.877-880
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    • 1999
  • Over 50 morphologically distinctive soil Streptomyces were isolated from various Jocations in the Yongin area in Korea and visually screened for dye-decoloring activities on an agar plate. Two Streptomyces species (AD001 and ND002) showed strong dye-decoloring activities on the plate containing congo-red and new-fuchin dyes, respectively. Also, the liquid culture supernatants of these species showed 2,4-dicholophenol (DCP) oxidation activities only in the presence of hydrogen peroxide, a characteristic of Actinomycetes lignin-peroxidase (ALiP)-P3 isoform found in dye-degrading S. viridosporus T7A and S. badius 252. Based on their dye-decoloring capabilities and the 2,4-DCP oxidation kinetic data, it is suggested that these Streptomyces secrete not-yet-characterized extracelluar enzyme(s), whose activities are very similar to the ALiP-P3 enzyme.

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Decolorization of Aqueous Caprolactam Solution by Anion-exchange Resins

  • Yuan Zhen;Yu Ping;Luo Yunbai
    • Fibers and Polymers
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    • v.7 no.2
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    • pp.112-116
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    • 2006
  • Caprolactam is the most important raw material for making Nylon 6 fibers and its quality directly determines the quality of Nylon. So it is necessary to study the techniques and methods to remove the colorful impurities from caprolactam. In this paper, the decolorization of caprolactam aqueous solution by anion exchange resins was studied and the decoloring abilities of five commercial resins were investigated. The regeneration of the resins was also studied, too. This study shows that the resin AMTX202 have excellent decoloring ability in the column experiment and that the decoloring efficiency is correlated with the volume of resins packed and is slightly affected by the flow rate and regenerating times. The fact that the resins can be regenerated and reused without affecting the efficiency of decolorization will decrease the cost of the treatment and operation in the industry. The adsorption of colored compounds with anion exchange resins in the packed columns seems to be technically feasible.

Utilization of Polyunsaturated Lipids in Red Muscled Fishes 3. The Conditions of Refining, Decoloring, and Deodorization for Processing of Refined Sardine Oil (적색육어류의 고도불포화지질의 이용에 관한 연구 3. 정제정어리유의 제조)

  • LEE Kang-Ho;JEONG In-Hak;SUH Jae-Soo;JUNG Woo-Jin;RYUK Ji-Hee
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.21 no.4
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    • pp.225-231
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    • 1988
  • As the studies on effective utilization of polyunsaturated lipids in sardine (Sardinops melanosticta), the conditions of deacidification, decoloring, and deodorization for processing of refined sardine oil were investigated In the process of refining, phosphoric acid treatment was not effective in removing phosphatides, and optimal condition to neutralize the crude sardine ill was treating for 30min at $40^{\circ}C$. with $0.5\%$ excess of 3M NaOH solution. Decoloring was optimized by adding $5\%$ bleaching earth and treating for 20min at $50^{\circ}C$ under vacuum, and deodorizing was done by steam destillation at $180^{\circ}C$ under 3 torr of vacuum. When deodorizing temperature exceeded $200^{\circ}C$, some changes occured in fatty acid composition and artifacts were appeared on GLC chromatogram.

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Fabrication of SnO2-based All-solid-state Transmittance Variation Devices (SnO2 기반 고체상의 투과도 가변 소자 제조)

  • Shin, Dongkyun;Seo, Yuseok;Lee, Jinyoung;Park, Jongwoon
    • Journal of the Semiconductor & Display Technology
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    • v.19 no.3
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    • pp.23-29
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    • 2020
  • Electrochromic (EC) device is an element whose transmittance is changed by electrical energy. Coloring and decoloring states can be easily controlled and thus used in buildings and automobiles for energy saving. There exist several types of EC devices; EC using electrolytes, polymer dispersed liquid crystal (PDLC), and suspended particle device (SPD) using polarized molecules. However, these devices involve solutions such as electrolytes and liquid crystals, limiting their applications in high temperature environments. In this study, we have studied all-solid-state EC device based on Tin(IV) oxide (SnO2). A coloring phase is achieved when electrons are accumulated in the ultraviolet (UV)-treated SnO2 layer, whereas a decoloring mode is obtained when electrons are empty there. The UV treatment of SnO2 layer brings in a number of localized states in the bandgap, which traps electrons near the conduction band. The SnO2-based EC device shows a transmittance of 70.7% in the decoloring mode and 41% in the coloring mode at a voltage of 2.5 V. We have achieved a transmittance change as large as 29.7% at the wavelength of 550 nm. It also exhibits fast and stable driving characteristics, which have been demonstrated by the cyclic experiments of coloration and decoloration. It has also showed the memory effects induced by the insulating layer of titanium dioxide (TiO2) and silicone (Si).

The feature of Microcapsule Involving Ultraviolet Rays Absorbent

  • Ueda, Yuka;Segawa, Akihiro;Murakoshi, Noriyuki;Hayashi, Natsuko;Yoshioka, Masato
    • Proceedings of the SCSK Conference
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    • 2003.09a
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    • pp.417-430
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    • 2003
  • A new method was developed to prepare microcapsules involving hydrophobic components. A totally new "silicone-resin-polypeptide" was used as the wall materials. The polypeptide was made by hydrolysis of collagen and silk protein and so on, and that was combined with silicone. This microcapsule was easily prepared from silicone-resin-polypeptide in water solution. The ratio of encapsulation in the microcapsule was not only high level as 90%, which had never been reached, but also the particle size could be controlled to obtain very small size (average particle size: 2${\mu}{\textrm}{m}$). Moreover, these microcapsules were resistant to high shearing forces and were stable over a long time period. This stable microcapsule was not crushed in pressure with finger spreading, so the core materials hardly touch the skin directly. Application in cosmetics by using microcapsule involving UV absorbents (2-ethylhexy1-4-methoxycinnamate (OMC) and 4-tert-butyl-4' -methoxydibenzoyl-methane (BMDBM)) was examined. It was possible to apply organic UV absorbents in water-rich formulations without any surfactant by using this microcapsule. This formulation demonstrated a good moisturizing and soft skin feel. Therefore, the microcapsule was applied to hair care products. As a result, the sunscreen hair lotion with microcapsule was able to prevent from damaging and decoloring of hair color by UV rays. As just, it was suggested that this microcapsules were be widely applied in cosmetics.cosmetics.

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Development of Polymeric Adsorbents for the Treatment of Colored Waste Waters and It's Applications ―Diethylaminoethylated Cellulosic Adsorbents― (유색배수 처리를 위한 고분자흡착제의 개발과 그 응용(III) ―디에틸아미노에틸화 셀룰로오스계 흡착제―)

  • Choe, Ji Eun;Sung, Woo Kyung;Lee, Mee Kyung;Park, Soo Min
    • Textile Coloration and Finishing
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    • v.5 no.4
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    • pp.42-48
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    • 1993
  • In the present work, decoloring of acid dye solution by the diethylaminoethylated cellulosic adsorbents($CA_{DASE}$) was studied with the aim of developing polymeric adsorbents for the treatment of colored wastewaters. To prepare the cellulosic adsorbents, the $CA_{DASE}$ cellulose and polyvinyl alcohol mixture(80 wt% cellulose content) were crosslinked by tryacryloyl hexahydro-s-tryazine(TAHHT), ammonium phosphate and then treated with solutions containing sodium hydroxide and 2-diethylaminoethyl chloride. Batch and flow method were employed to determine decoloring capacity of C $A_{DEAE}$ for C.I.acid yellow 49. $CA_{DASE}$ exhibited much better desorption capacity than activated carbon. Furthermore, the exhausted $$CA{DASE} could be readily regenerated by washing with dilute sodium hydroxide.

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Decoloring Effect of Viscose Rayon by Using Vapor Type Ozone Processing (오존 기상처리방법에 의한 비스코스 레이온의 탈색효과)

  • Kim, Gyoung-A;Lee, Mun-Soo
    • The Journal of Natural Sciences
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    • v.10 no.1
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    • pp.103-109
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    • 1998
  • We studied on the decoloring effect of viscose rayon by vapor type ozone processing using ozone's strong oxidation. When ozone is generated, it becomes high concentration in vapor state, but in the insolving process with water, there are a lot of lose of ozone. As a result, a study using ozone in vapor-high concentration state is needed. So, in this paper, vapor type ozone processing is used unlike previous ozone treatment method-an aqueous solution method-to get a good effect from shortening the processing time. When vapor type ozone processing was directly treated to fabrics, high concentration ozone was generated then in a short time oxidized a dye existed in fabrics and finally decolored it. As vapor type ozone did not directly response to an organic dye, viscose rayon's decolorization was to be studied by changing pick up ratio(60%, 50%, 40%, 30%, 20%) using water as a medium. When pick up ratio of water was 40%, fabric's whiteness was improved but tensile strength and elongation were reduced. So 60 minutes was assumed as the most adjust time to minimize the reduction of fabric's tensile strength and elongation moreover of maximize the improvement of whiteness.

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