• 제목/요약/키워드: decoloring

검색결과 23건 처리시간 0.017초

Hydrogen Peroxide, Its Measurement and Effect During Enzymatic Decoloring of Congo Red

  • Woo, Sung-Whan;Cho, Jeung-Suk;Hur, Byung-Ki;Shin, Dong-Hoon;Ryu, Keun-Gap;Kim, Eun-Ki
    • Journal of Microbiology and Biotechnology
    • /
    • 제13권5호
    • /
    • pp.773-777
    • /
    • 2003
  • The color of Congo red hinders the spectrometric measurements of a concentration of hydrogen peroxide and enzyme activity (Horseradish peroxidase; HRP) during enzymatic decoloring of Congo red. In this study, a method was developed to measure peroxidase activity and hydrogen peroxide concentration in the presence of Congo red. The oxidation product of HRP/hydrogen peroxide and ABTS(2,2'-azino-bis-(3-ethylbenzotriazoline-6-sulfonic acid)) formed a dark green color. The spectrum of this product showed absorption bands at 420 nm and 734 nm. When compared with the Congo red spectrum, the absorption at 734 nm of this product did not overlap with Congo red, thus making the hydrogen peroxide measurement possible even in the presence of Congo red. Kinetic study of decoloring of Congo red performed by this method showed that the decoloring reaction followed the Michaelis-Menten kinetics. Pulse feeding of hydrogen peroxide, upon depletion, significantly increased the decoloring of Congo red. This result shows that this newly developed technique can monitor, predict, and improve the enzymatic decoloring process.

Hydrogen peroxide, its measurement and effect during enzymatic decoloring of Congo Red

  • 우성환;조정숙;김갑정;김은기
    • 한국생물공학회:학술대회논문집
    • /
    • 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
    • /
    • pp.194-197
    • /
    • 2000
  • The color of textile-wastewater hindered spectrometric measurements of $H_2O_2$ and enzyme activity during enzymatic decoloring. By using ABTS, we developed a new method for measuring peroxidase activity and $H_2O_2$ concentration. The ratio of enzyme and $H_2O_2$ was optimized as 1:150 by investigating the effects of $H_2O_2$ on enzymatic decoloring. Pulse feeding of $H_2O_2$, upon depletion, significantly increased the decoloring of Congo Red.

  • PDF

발화력지수(醱化力指數)가 유리색조(色調)에 미치는 영향(影響) (The Effects of Glass Color Tone by Oxidation Power Index)

  • 박은성;박병기
    • 품질경영학회지
    • /
    • 제16권1호
    • /
    • pp.1-7
    • /
    • 1988
  • The most important element upon the quality of family glass products is the color of glass, which is the first object of consumers concern in purchasing. Therefore, colorless and transparent color tone can level up the additional value of the products. But we have produced the glass products in a large quantity by using the automatic tank brazier, chronic problem has been left over the color tone. By compounding and analizing the management techniques applied by peculiar technique and design of experiments pertinently, and by adjusting the decoloring composition ratio, we can contribute to improvement of color tone as well as to the cost reduction by diminishing the unnecessary decoloring agent. Intangible effect was the securing the superiority of quality home and abroad with the improvement of color tone and was taking a triangular position the factors influencing the glass decoloring which had been an unexhausted field. Tangible effect was the reduction of decoloring cost by diminishing the unnecessary oxidant. We registered the optimal composition ratio condition which was searched by applicating the design of experiments. To maintain good color tone, we manage the degree of purity, the most important thing among the items of color tone, continuously by control chart and it has maintained steady state now.

  • PDF

Isolation and Characterization of Soil Streptomyces Involved in 2,4-Dichlorophenol Oxidation

  • Kang, Min-Jin;Kang, Ja-Kyoung;Kim, Eung-Soo
    • Journal of Microbiology and Biotechnology
    • /
    • 제9권6호
    • /
    • pp.877-880
    • /
    • 1999
  • Over 50 morphologically distinctive soil Streptomyces were isolated from various Jocations in the Yongin area in Korea and visually screened for dye-decoloring activities on an agar plate. Two Streptomyces species (AD001 and ND002) showed strong dye-decoloring activities on the plate containing congo-red and new-fuchin dyes, respectively. Also, the liquid culture supernatants of these species showed 2,4-dicholophenol (DCP) oxidation activities only in the presence of hydrogen peroxide, a characteristic of Actinomycetes lignin-peroxidase (ALiP)-P3 isoform found in dye-degrading S. viridosporus T7A and S. badius 252. Based on their dye-decoloring capabilities and the 2,4-DCP oxidation kinetic data, it is suggested that these Streptomyces secrete not-yet-characterized extracelluar enzyme(s), whose activities are very similar to the ALiP-P3 enzyme.

  • PDF

Decolorization of Aqueous Caprolactam Solution by Anion-exchange Resins

  • Yuan Zhen;Yu Ping;Luo Yunbai
    • Fibers and Polymers
    • /
    • 제7권2호
    • /
    • pp.112-116
    • /
    • 2006
  • Caprolactam is the most important raw material for making Nylon 6 fibers and its quality directly determines the quality of Nylon. So it is necessary to study the techniques and methods to remove the colorful impurities from caprolactam. In this paper, the decolorization of caprolactam aqueous solution by anion exchange resins was studied and the decoloring abilities of five commercial resins were investigated. The regeneration of the resins was also studied, too. This study shows that the resin AMTX202 have excellent decoloring ability in the column experiment and that the decoloring efficiency is correlated with the volume of resins packed and is slightly affected by the flow rate and regenerating times. The fact that the resins can be regenerated and reused without affecting the efficiency of decolorization will decrease the cost of the treatment and operation in the industry. The adsorption of colored compounds with anion exchange resins in the packed columns seems to be technically feasible.

적색육어류의 고도불포화지질의 이용에 관한 연구 3. 정제정어리유의 제조 (Utilization of Polyunsaturated Lipids in Red Muscled Fishes 3. The Conditions of Refining, Decoloring, and Deodorization for Processing of Refined Sardine Oil)

  • 이강호;정인학;서재수;정우진;육지희
    • 한국수산과학회지
    • /
    • 제21권4호
    • /
    • pp.225-231
    • /
    • 1988
  • 정어리유의 고도불포화지질을 일상식생활을 통하여 효과적으로 이용하기 위하여 정제정어리건의 제조를 위한 탈산, 탈색, 탈취 등의 정제조건을 검토한 결과를 요약하면 다음과 같다. 1. 정어리유의 탄산은 알칼리처리가 효과적이었으며 2M NaOH용액을 $0.5\%$ 과잉첨가하고 $30^{\circ}C$, 30분처리가 적조건이었다. 일반적으로 행하는 인산처리는 탈검에 효과가 없었다. 2. 탈색은 산성백토만을 기름의 $5\%$정도로 첨가하고 감압하에서 $60^{\circ}C$ 20분간 처리하는 것이 가장 효락적이었다. 3. 탈취는 수증기증류가 효과적이었으며 4torr 이하로 감압하면서 $180^{\circ}C$ 이하의 온도에서 하는 것이 바람직하였고 $200^{\circ}C$ 이상에서는 현저한 요오드가의 감소와 지방산조성에 변화를 초래하였다.

  • PDF

SnO2 기반 고체상의 투과도 가변 소자 제조 (Fabrication of SnO2-based All-solid-state Transmittance Variation Devices)

  • 신동균;서유석;이진영;박종운
    • 반도체디스플레이기술학회지
    • /
    • 제19권3호
    • /
    • pp.23-29
    • /
    • 2020
  • Electrochromic (EC) device is an element whose transmittance is changed by electrical energy. Coloring and decoloring states can be easily controlled and thus used in buildings and automobiles for energy saving. There exist several types of EC devices; EC using electrolytes, polymer dispersed liquid crystal (PDLC), and suspended particle device (SPD) using polarized molecules. However, these devices involve solutions such as electrolytes and liquid crystals, limiting their applications in high temperature environments. In this study, we have studied all-solid-state EC device based on Tin(IV) oxide (SnO2). A coloring phase is achieved when electrons are accumulated in the ultraviolet (UV)-treated SnO2 layer, whereas a decoloring mode is obtained when electrons are empty there. The UV treatment of SnO2 layer brings in a number of localized states in the bandgap, which traps electrons near the conduction band. The SnO2-based EC device shows a transmittance of 70.7% in the decoloring mode and 41% in the coloring mode at a voltage of 2.5 V. We have achieved a transmittance change as large as 29.7% at the wavelength of 550 nm. It also exhibits fast and stable driving characteristics, which have been demonstrated by the cyclic experiments of coloration and decoloration. It has also showed the memory effects induced by the insulating layer of titanium dioxide (TiO2) and silicone (Si).

The feature of Microcapsule Involving Ultraviolet Rays Absorbent

  • Ueda, Yuka;Segawa, Akihiro;Murakoshi, Noriyuki;Hayashi, Natsuko;Yoshioka, Masato
    • 대한화장품학회:학술대회논문집
    • /
    • 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
    • /
    • pp.417-430
    • /
    • 2003
  • A new method was developed to prepare microcapsules involving hydrophobic components. A totally new "silicone-resin-polypeptide" was used as the wall materials. The polypeptide was made by hydrolysis of collagen and silk protein and so on, and that was combined with silicone. This microcapsule was easily prepared from silicone-resin-polypeptide in water solution. The ratio of encapsulation in the microcapsule was not only high level as 90%, which had never been reached, but also the particle size could be controlled to obtain very small size (average particle size: 2${\mu}{\textrm}{m}$). Moreover, these microcapsules were resistant to high shearing forces and were stable over a long time period. This stable microcapsule was not crushed in pressure with finger spreading, so the core materials hardly touch the skin directly. Application in cosmetics by using microcapsule involving UV absorbents (2-ethylhexy1-4-methoxycinnamate (OMC) and 4-tert-butyl-4' -methoxydibenzoyl-methane (BMDBM)) was examined. It was possible to apply organic UV absorbents in water-rich formulations without any surfactant by using this microcapsule. This formulation demonstrated a good moisturizing and soft skin feel. Therefore, the microcapsule was applied to hair care products. As a result, the sunscreen hair lotion with microcapsule was able to prevent from damaging and decoloring of hair color by UV rays. As just, it was suggested that this microcapsules were be widely applied in cosmetics.cosmetics.

  • PDF

유색배수 처리를 위한 고분자흡착제의 개발과 그 응용(III) ―디에틸아미노에틸화 셀룰로오스계 흡착제― (Development of Polymeric Adsorbents for the Treatment of Colored Waste Waters and It's Applications ―Diethylaminoethylated Cellulosic Adsorbents―)

  • Choe, Ji Eun;Sung, Woo Kyung;Lee, Mee Kyung;Park, Soo Min
    • 한국염색가공학회지
    • /
    • 제5권4호
    • /
    • pp.42-48
    • /
    • 1993
  • In the present work, decoloring of acid dye solution by the diethylaminoethylated cellulosic adsorbents($CA_{DASE}$) was studied with the aim of developing polymeric adsorbents for the treatment of colored wastewaters. To prepare the cellulosic adsorbents, the $CA_{DASE}$ cellulose and polyvinyl alcohol mixture(80 wt% cellulose content) were crosslinked by tryacryloyl hexahydro-s-tryazine(TAHHT), ammonium phosphate and then treated with solutions containing sodium hydroxide and 2-diethylaminoethyl chloride. Batch and flow method were employed to determine decoloring capacity of C $A_{DEAE}$ for C.I.acid yellow 49. $CA_{DASE}$ exhibited much better desorption capacity than activated carbon. Furthermore, the exhausted $$CA{DASE} could be readily regenerated by washing with dilute sodium hydroxide.

  • PDF

오존 기상처리방법에 의한 비스코스 레이온의 탈색효과 (Decoloring Effect of Viscose Rayon by Using Vapor Type Ozone Processing)

  • 김경아;이문수
    • 자연과학논문집
    • /
    • 제10권1호
    • /
    • pp.103-109
    • /
    • 1998
  • 본 연구는 오존의 강한 산화력을 이용하여 기상의 처리방법에 따른 비스코스 레이온의 탈색효과를 연구하였다. 오존이 발생할 때는 기체상태로 고농도이지만, 수용액 상태로 물에 녹이는 과정에서 기화요인에 의하여 오존의 손실이 많았다. 따라서 기존의 오존처리방법인 수용액상태의 처리방법이 아니라 기상의 오존을 직접 직물에 처리하므로 고농도의 오존을 단시간동안 처리하여 직물에 존재하는 색소를 산화시켜 탈색하는 방법을 사용하였다. 기상의 오존은 직접 유기색소와 반응하지 않기 때문에 물을 매개체로 하여 pick up ratio를 60%, 50%, 40%, 30%, 20%로 변화시키면서 비스코스 레이온의 탈색에 대하여 연구하였다. Pick up ratio가 40%일 때가 유기색소를 분해하는데 필요한 최적의 수산기가 발생하므로 처리포의 탈색효과가 가장 좋았다. 그러나 처리기간이 길수록 너무 과다하게 오존에 의해 산화되므로 강도와 신도의 저하를 초래하였다. 따라서 최적의 기상오존 처리시간은 60분정도에서 처리하는 것이 강도와 신도의 저하를 가장 적게 할 수 있고 백도도 향상시킬 수 있는 것으로 사료된다.

  • PDF