• Journal of Microbiology and Biotechnology
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• v.14 no.6
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• pp.1343-1349
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• 2004

A native strain of Pasteurella multocida was isolated from pigs suffering from severe atrophic rhinitis at domestic farms in Gyeonggi Province, Korea, and was identified as capsular serogroup 'D' and somatic serotype '4' by disc diffusion decapsulation and gel diffusion precipitation tests, respectively. The P. multocida (D:4) induced atrophic rhinitis in healthy pigs by the secondary infection. The gene for outer membrane protein H (ompH) of P. multocida (D:4) was cloned in Escherichia coli DH5$\alpha$ by PCR. The open reading frame of the ompH was composed of 1,023 bp, possibly encoding a protein with 341 amino acid residues containing a signal peptide of 20 amino acids at N-terminus, and the gene product with molecular mass of ca. 38 kDa was identified by SDS-PAGE. Hydropathy profiles indicated that there are two variable domains in the OmpH. To express the ompH in E. coli, the gene was manipulated in various ways. Expression of the truncated as well as full-length forms of the recombinant OmpH was fatal to the host E. coli BL21 (DE3). However, the truncated OmpH fused with GST was consecutively expressed in E. coli DH5$\alpha$. A large quantity of the fused polypeptide was purified through GST-affinity chromatography.

• Journal of KIISE:Computing Practices and Letters
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• v.9 no.5
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• pp.578-587
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• 2003

The DSTM (Dual Stack Transition Mechanism), one of tunneling mechanism, is considered as the best solution in IPv4/IPv6 transition recently. The DSTM provides a method to assure IPv4/v6 connectivity based on 4over6 (IPv4-over-IPv6) tunneling and temporal allocation of a global IPv4 address to a host requiring such communication. A TEP (Tunnel End Point) operates as a border router between IPv6 domain and IPv4 Internet, which performs encapsulation and decapsulation of 4over6 tunneling packets to assure hi-directional forwarding between both networks. In this paper, we analyze basic standards of the IPv6 protocol. And, we design and implement a DSTM TEP daemon block. The TEP daemon analyzes a fevers tunneling packet that is forwarded by the DSTM node, establishes the TEP's 4over6 interface, and supplies communication between a DSTM and a IPv4-only node. Finally, we construct a DSTM testbed and measure performance of the DSTM TEP. Our observation results show that performance of TEP supports the DSTM service.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.5
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• pp.1053-1059
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• 2011

DMX512 protocol is a typical protocol which controls LED lighting system. It has a strong feature and can be used to a lot of lighting system. But LED control system with cable has a few problem because they have a lot of control cables. It is very difficult to set up the complex lighting. Furthermore if it is set up it is not easy to keep and recover the system from the out of order. So it is required Wireless Lighting Control System. This paper describes the proposal, design, and implementation of a novel method which can provide versatile light effects with the increased capability of the mobility and the number of universes for Wireless Lighting system. And then we implement experiment environment to control indoor lighting system controled by ZigBee and WLAN converter not having complicating control line. Data frames are transferred to DMX512 device by encapsulation and decapsulation to control color LED. In the same time give the method to solve the data bottleneck problem which can be comes out because of speed difference between Heterogeneous network and measure performance of control system.