• Title/Summary/Keyword: de-composition

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Evaluation of Energy Digestibility and Prediction of Digestible and Metabolizable Energy from Chemical Composition of Different Cottonseed Meal Sources Fed to Growing Pigs

  • Li, J.T.;Li, D.F.;Zang, J.J.;Yang, W.J.;Zhang, W.J.;Zhang, L.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.10
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    • pp.1430-1438
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    • 2012
  • The present experiment was conducted to determine the digestible energy (DE), metabolizable energy (ME) content, and the apparent total tract digestibility (ATTD) of energy in growing pigs fed diets containing one of ten cottonseed meals (CSM) collected from different provinces of China and to develop in vitro prediction equations for DE and ME content from chemical composition of the CSM samples. Twelve growing barrows with an initial body weight of $35.2{\pm}1.7$ kg were allotted to two $6{\times}6$ Latin square designs, with six barrows and six periods and six diets for each. A corn-dehulled soybean meal diet was used as the basal diet, and the other ten diets were formulated with corn, dehulled soybean meal and 19.20% CSM. The DE, ME and ATTD of gross energy among different CSM sources varied largely and ranged from 1,856 to 2,730 kcal/kg dry matter (DM), 1,778 to 2,534 kcal/kg DM, and 42.08 to 60.47%, respectively. Several chemical parameters were identified to predict the DE and ME values of CSM, and the accuracy of prediction models were also tested. The best fit equations were: DE, kcal/kg DM = 670.14+31.12 CP+659.15 EE with $R^2$ = 0.82, RSD = 172.02, p<0.05; and ME, kcal/kg DM = 843.98+25.03 CP+673.97 EE with $R^2$ = 0.84, RSD = 144.79, p<0.05. These results indicate that DE, ME values and ATTD of gross energy varied substantially among different CSM sources, and that some prediction equations can be applied to predict DE and ME in CSM with an acceptable accuracy.

Analysis of Factors Affecting the Periplasmic Production of Recombinant Proteins in Escherichia coli

  • Mergulhao, Filipe J.;Monteiro, Gabriel A.
    • Journal of Microbiology and Biotechnology
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    • v.17 no.8
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    • pp.1236-1241
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    • 2007
  • Five fusion proteins between Z domains derived from Staphylococcal Protein A and Green Fluorescent Protein or Human Proinsulin were produced on the periplasm of Escherichia coli. The effects of the molecular weight and amino acid composition of the translocated peptide, culture medium composition, and growth phase of the bacterial culture were analyzed regarding the expression and periplasmic secretion of the recombinant proteins. It was found that secretion was not affected by the size of the translocated peptide (17-42 kDa) and that the highest periplasmic production values were obtained on the exponential phase of growth. Moreover, the highest periplasmic values were obtained in minimal medium, showing the relevance of the culture medium composition on secretion. In silico prediction analysis suggested that with respect to the five proteins used in this study, those that are prone to form ${\alpha}$-helix structures are more translocated to the periplasm.

Modelling the Hydrogen-Bonding Interactions in a Copolymer/Biodegradable Homopolymer Blend through Excess Functions

  • Garcia-Lopera, Rosa;Monzo, Isidro S.;Campos, Agustin;Abad, Concepcion
    • Macromolecular Research
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    • v.16 no.5
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    • pp.446-456
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    • 2008
  • A recent theoretical approach based on the coupling of both the Flory-Huggins (FH) and the Association Equilibria thermodynamic (AET) theories was modified and adapted to study the miscibility properties of a multi-component system formed by two polymers (a proton-donor and a proton-acceptor) and a proton-acceptor solvent, named copolymer(A)/solvent(B)/polymer(C). Compatibility between polymers was mainly attained by hydrogen-bonding between the hydroxyl group on the phenol unit of the poly(styrene-co-vinyl phenol) (PSVPh) and the carbonyl group of the biodegradable and environmentally friendly poly(3-hydroxybutyrate) (PHB). However, the self-association of PSVPh and specific interactions between the PSVPh and the H-acceptor group (an ether oxygen atom) of the epichlorohydrin (ECH) solvent were also established in a lower extension, which competed with the polymer-polymer association. All the binary specific interactions and their dependence with the system composition as well as with the copolymer content were evaluated and quantified by means of two excess functions of the Gibbs tree energy, ${\Delta}g_{AB}$ and ${\Delta}g_{AC}$. Experimental results from fluorescence spectroscopy were consistent with the theoretical simulations derived with the model, which could also be applied and extended to predict the miscibility in solution of any polymer blend with specific interactions.

Effect of Composition Size of Sintered Body and Electrode on Resonant Characteristics of $Pb(Mn_{1/3}Nb_{2/3})O_3 PbTiO_3-PbZrO_3$ Piezoelectric Ceramics (소결체 및 전극의 크기와 조성이 $Pb(Mn_{1/3}Nb_{2/3})O_3 PbTiO_3-PbZrO_3$계 압전세라믹스의 공진특성에 미치는 영향)

  • 류영대;조상희
    • Journal of the Korean Ceramic Society
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    • v.23 no.1
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    • pp.60-66
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    • 1986
  • The effect of composition size of sintered body and electrode on resonant characteristics of the system $Pb(Mn_{1/3}Nb_{2/3})O_3 PbTiO_3-PbZrO_3$ has been decribed. Composition ranged from X=40 to X=55 diameter of sintered body ranged from D=6.5 to D=12.5(mm) and diameter of electrode ranged from De=5.5 to De=11 (mm) In the composition of morphotropic phase boundary antiresonant frequency (fa) dcreased slowly whereas resonant frequency (fr) decreased rapidly on the ground of this Δf(fa-fr) and electromechanical coupling fractor Kp increased and Qm showed low value. On the contrary in toward the composition of tetragonal and rhombo-hedral fa increased slowly whereas fr increased rapidly on the ground of this Δf and Kp decreased and Qm increased abruptly. Substance of the above statements have no concern with size of sintered body and ele-ctrode. The other side as the size of electrode decreased Qm fr, fa and Δf increased. Cm and Co dominantly affect the resonant frequency and antiresonant frequency.

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DeNitrification-DeComposition (DNDC) Improvement through Model Coupling and Sub-model Development Considering Agricultural Land Use and Future Climate Change

  • Min, Hyungi;Hwang, Wonjae;Kim, Min-Suk;Kim, Jeong-Gyu
    • Korean Journal of Environmental Biology
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    • v.35 no.1
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    • pp.37-46
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    • 2017
  • Climate change is the biggest concern of the $21^{st}$ century. Greenhouse gas (GHG) emissions from various sectors are attracting attention as a cause of climate change. The DeNitrification-DeComposition (DNDC) model simulates GHG emissions from cropland. To study future GHG emissions using this simulation model, various factors that could change in future need to be considered. Because most problems are from the agricultural sector, DNDC would be unable to solve the factor-changing problem itself. Hence, it is necessary to link DNDC with separate models that simulate each element. Climate change is predicted to cause a variety of environmental disasters in the future, having a significant impact on the agricultural environment. In the process of human adaptation to environmental change, the distribution and management methods of farmland will also change greatly. In this study, we introduce some drawbacks of DNDC in considering future changes, and present other existing models that can rectify the same. We further propose some combinations with models and development sub-models.

Prediction equations for digestible and metabolizable energy concentrations in feed ingredients and diets for pigs based on chemical composition

  • Sung, Jung Yeol;Kim, Beob Gyun
    • Animal Bioscience
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    • v.34 no.2
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    • pp.306-311
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    • 2021
  • Objective: The objectives were to develop prediction equations for digestible energy (DE) and metabolizable energy (ME) of feed ingredients and diets for pigs based on chemical composition and to evaluate the accuracy of the equations using in vivo data. Methods: A total of 734 data points from 81 experiments were employed to develop prediction equations for DE and ME in feed ingredients and diets. The CORR procedure of SAS was used to determine correlation coefficients between chemical components and energy concentrations and the REG procedure was used to generate prediction equations. Developed equations were tested for the accuracy according to the regression analysis using in vivo data. Results: The DE and ME in feed ingredients and diets were most negatively correlated with acid detergent fiber or neutral detergent fiber (NDF; r = -0.46 to r = -0.67; p<0.05). Three prediction equations for feed ingredients reflected in vivo data well as follows: DE = 728+0.76×gross energy (GE)-25.18×NDF (R2 = 0.64); ME = 965+0.66×GE-24.62×NDF (R2 = 0.60); ME = 1,133+0.65×GE-29.05×ash-23.17×NDF (R2 = 0.67). Conclusion: In conclusion, the equations suggested in the current study would predict energy concentration in feed ingredients and diets.

Purification and Characterization of Two Isolectins with Arginase Activity from the Lichen Xanthoria parietina

  • Molina, M. C.;Vicente, C.
    • BMB Reports
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    • v.33 no.4
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    • pp.300-307
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    • 2000
  • Two glycoproteins were purified and biochemically characterized from the lichen X. parietina. Both behaved as enzymes with arginase activity and haemaglutinins. Secreted arginase (SA) contained galactose and glucose in the saccharide moiety and an isoelectric point of 4.54. The algal binding-protein (ABP) had N-acetyl-glucosamine and glucose as glycosidic residues and an isoelectric point of 3.53. Both proteins had the same molecular mass (58.6 kDa) and the same qualitative amino acidic composition. The results allowed us to consider these glycoproteins as isolectins, which have significant physiological roles in the relationship between photobiont and mycobiont of symbiotic association.

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Adhesion of aluminium alloys: morphology, surface chemistry and adhesive bond durability

  • Vaslier, L.;Moutarlier, V.;Gigandet, M.P.;Grisel, M.
    • Proceedings of the Polymer Society of Korea Conference
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    • 2006.10a
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    • pp.362-362
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    • 2006
  • Chromic acid anodizing of aluminium is an effective process for producing a substrate which provides excellent surface properties to assembly. However, new chromium-free finishing metal treatments are being developed to reach new environmental goals. Present study deals with chromium free experimental conditions for both deoxidizing and anodizing treatments. Results clearly demonstrate the potentiality for environmentally friendly treatments to get suitable metal-adhesive performance. In particular, the role of both metal surface porosity and chemical composition has been highlighted thus allowing to propose alternative to classical treatments for aeronautic applications.

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Powder Injection Molding of Alumina Parts Using a Binder System Based in Paraffin Wax and High Density Polyethylene

  • Thomas-Vielma, P.;Cervera, A.;Levenfeld, B.;Varez, A.
    • Proceedings of the Korean Powder Metallurgy Institute Conference
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    • 2006.09a
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    • pp.207-208
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    • 2006
  • In this experimental work, the development of a multicomponent binder system based on high density polyethylene (HDPE) and paraffin wax for Powder Injection Molding of Alumina $(Al_2O_3)$ parts was carried out. The optimum composition of the injection mixture was established through mixing torque measurements and a rheological study. The maximum powder loading was 58 vol%. The miscibility of organic components and the optimum injection temperature was evaluated by thermal characterization of binder and feedstock. The thermal debinding cycle was developed on the basis of thermogravimetrical analysis of the binder. After sintering the densities achieved were closed to 98% of the theoretical one.

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Structure and Function of the Genes Involved in the Biosynthesis of Carotenoids in the Mucorales

  • Iturriaga, Enrique A.;Velayos, Antonio;Eslava, Arturo P.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.4
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    • pp.263-274
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    • 2000
  • Carotenoids are widely distributed natural pigments which are in an increasing demand by the market, due to their applicatins in the human food, animal feed, cosmetics, and pharmaceutical industries. Although more than 600 carotenoids have been identified in nature, only a few are industrially important (${\beta}$-carotene, astaxanthin, lutein or lycopene). To date chemical processes manufacture most of the carotenoid production, but the interest for carotenoids of biological origin is growing since theire is an increased public concern over the safety of artificial food colorants. Although much interest and effort has been devoted to the use of biological sources for industrially important carotenoids, only the production of biological ${\beta}$-carotene and astaxanthin has been reported. Among fungi, several Mucorales strains, particularly Blakeslea trispora, have been used to develop fermentation processes for the production of ${\beta}$-carotene on almost competitive cost-price levels. Similarly, the basidiomycetous yeast Xanthophyllomyces dendrorhous (the perfect state of Phaffia rhodozyma), has been proposed as a promising source of astaxanthin. This paper focuses on recent findings on the fungal pathways for carotenoid production, especially the structure and function of the genes involved in the biosynthesis of carotenoids in the Mucorales. An outlook of the possibilities of an increased industrial production of carotenoids, based on metabolic engineering of fungi for carotenoid content and composition, is also discussed.

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