• Research in Plant Disease
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• 제21권4호
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• pp.315-320
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• 2015

Soybean mosaic virus (SMV) is a prevalent pathogen that causes significant yield reduction in soybean production worldwide. SMV belongs to potyvirus and causes typical symptoms such as mild mosaic, mosaic and necrosis. SMV is seed-borne and also transmitted by aphid. Eleven SMV strains, G1 to G7, G5H, G6H, G7H, and G7a were reported in soybean varieties in Korea. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SML-F3/B3/FIP/BIP from coat protein gene sequence of SMV. After the reaction of RT-LAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I under daylight and UV light. Optimal reaction condition was at $58^{\circ}C$ for 60 min and the primers of RT-LAMP showed the specificity for nine SMV strains tested in this study.

• Asian-Australasian Journal of Animal Sciences
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• 제21권10호
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• pp.1466-1472
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• 2008

Variations in conjugated linoleic acid (CLA) concentrations in Holstein dairy cows milk, depending on feeding systems in different seasons was investigated. Milk samples were collected from Holstein dairy cows, which either grazed for whole days (WG), only daylight hours (TG), or were offered a total mixed ration (TMR) and experienced no grazing (NG), from April to December of 2005. In April, November and December, the cows in TG and WG treatments received grass silage and some concentrate, while from May to October, the cows grazed on temperate pasture. The cows in NG treatment received the TMR throughout the season. The major fatty acid obtained in the pastures was linolenic acid. There was no significant difference in the pasture's linolenic acid concentrations from May to September, but there was a significant decrease in October. However, the linolenic acid concentrations obtained in the pasture were always much higher than those obtained from the TMR. Linoleic acid was also the major fatty acid in the TMR, but these concentrations were higher in the TMR than in the pasture. There was no significant difference in milk cis9trans11CLA (c9t11CLA) concentrations between the three feeding systems while the cows were fed on conserved pasture in April, November and December. Although c9t11CLA concentrations were lower in the TMR, it was found that the cows which grazed in fresh pasture experienced significantly higher concentrations of c9t11CLA in their milk than those which received only TMR. It was also found that cows in the WG treatment experienced higher c9t11CLA concentrations than those in the TG treatment. In the WG and TG treatments, c9t11CLA concentrations were highest in June, after which, they gradually decreased (p<0.01) until October. For the NG treatment, there was no significant change in the concentrations of c9t11CLA (p>0.05) with season. Overall, trans11C18:1 and c9t11CLA were greatly influenced by season, with higher variation in the WG treatment than in the TG treatment and no variation in the NG treatment.

• Asian-Australasian Journal of Animal Sciences
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• 제21권10호
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• pp.1411-1415
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• 2008

The purpose of the present study was to determine the onset of breeding season, the occurrence of silent and true heats and the duration of estrus in female Markhoz goats of the Kurdistan province in Iran. Ten, 3 years-old Markhoz does with an average weight of $34.05{\pm}2.62kg$ and with one kidding record, were used. The goats were maintained in an open barn under constant nutritional levels and natural photoperiod. One aproned buck was used twice a day every 12 h to detect estrus from mid August to early January. For the determination of the onset of reproductive activity as well as occurrence of silent heat, blood samples were collected every 10 days, from the beginning of the experiment. After $2^{nd}$ standing heat, blood samples were obtained twice a week in order to assess luteal activity and the length of estrous cycles. In this study, estrous behavior was observed including sniffing, vocal exchange, following courtship, flehman, standing heat and mounting. The results of the progesterone assay indicated that in this goat silent heats occur mostly in the early breeding season. The first standing heat was observed in mid-October which was considered as the onset of the breeding season. Duration of the estrous cycle and estrus was recorded as being $20.93{\pm}1.56days$ and $38.86{\pm}15.19h$, respectively. The correlation coefficient between length of daylight and occurrence of estrus was negative (r = -0.470) but not significant (p>0.05). The data showed that there was no significant effect of body weight on estrous cycles (first, second and third) and estrous periods (first, second, third and fourth). Progesterone levels were not significantly different in the first, second and third estrous cycles at days 0, 4, 10, 12, 14 and 19. The results of progesterone assay during the estrous cycle indicate that follicular and luteal phases last 4-5 and 14-15 days, respectively and the concentration of serum progesterone in these phases was $0.88{\pm}0.08$ and $7.44{\pm}0.26ng/ml$, respectively. The study concluded that Markhoz does could be considered as a breed with a short breeding season and an optimal estrous activity in autumn.

• Korean Journal of Fisheries and Aquatic Sciences
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• 제46권2호
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• pp.195-200
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• 2013

This study investigated the effects of water temperature, photoperiod and population density on oxygen consumption (OC) in the longtooth grouper (Epinephelus bruneus). OC rate in the longtooth grouper at 15, 20, and $25^{\circ}C$ were $85.9{\pm}6.9$, $107.5{\pm}10.1$, and $164.0{\pm}19.2\;mg\;O_2\;kg^{-1}\;h^{-1}$, respectively, indicating a linear increase in OC with water temperature. Photoperiod was regulated in accordance with the light (09:00-21:00 h, L) and dark (21:00-09:00 h, D) phases of the diel cycle (12L/12D), with a water temperature of 15, 20, or $25^{\circ}C$. OC rates during the light and dark phases were $83.8{\pm}5.4$, $88.1{\pm}7.8\;mg\;O_2\;kg^{-1}\;h^{-1}$, respectively, at $15^{\circ}C$ and $111.2{\pm}12.3$ and $103.7{\pm}5.7\;mg\;O_2\;kg^{-1}\;h^{-1}$ at $20^{\circ}C$. No significant differences were observed between the light and dark phases (P > 0.05). at $25^{\circ}C$ the OC rates were $168.8{\pm}24.3$ and $159.2{\pm}11.4\;mg\;O_2\;kg^{-1}\;h^{-1}$ during the light and dark phases, respectively, indicating that OC is higher during daylight than nighttime. OC tates at 55.4, 88.4, 118.8, and 145.1 g $L^{-1}$ were $252.0{\pm}11.6$, $219.0{\pm}8.7$, $206.7{\pm}11.4$, and $208.8{\pm}11.4\;mg\;O_2\;kg^{-1}\;h^{-1}$, respectively, indicating a decrease in OC with increasing population density. However, no significant difference was observed between the values for 118.8 g $L^{-1}$ and 145.1 g $L^{-1}$ (P > 0.05).

• Journal of Ecology and Environment
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• 제29권3호
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• pp.277-286
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• 2006

Population ecology of Cobitis tetralineata was examined at Churyeong Stream, Seomjin River, Korea. C. tetralineata inhabited on the sand bottoms with $10{\sim}20cm/see$ in current velocity, and $30{\sim}150cm$ in water depth. This species was active on the sand during the daylight hours from March to October, but they hibernated in the inside of the sand during the winter season. Sex ratio of female to male was 1:0.57, and female was $20{\sim}30mm$ (TL) larger than male. The age group of C. tetralineata (female) population showed that the $20{\sim}40mm$ group is 0+ years old, the $45{\sim}65mm$ group 1+ years old, the $65{\sim}90mm$ group 2+ years old, and the group longer than 90 mm over 3 years old. Males $13{\sim}14$ months old after hatching had lamina circularis at the base of its pectoral fin as a secondary sexual character. And in its spawning season, lateral color pattern of male was changed as a sexual dimorphism. The spawning season may be from late June to middle July, $22{\sim}26^{\circ}C$ water temperature. The average number of mature eggs in ovary was about $1,288{\pm}583(474{\sim}2,976)$, egg diameter was about $0.98{\pm}0.1mm$. C. tetralineata fed mainly on Chironomidae, Arcellidae, Branchioda and Algae. The feeding rate was the highest in April and September, but they did not fed in the winter.

• Journal of the Institute of Electronics Engineers of Korea SP
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• 제43권3호
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• pp.25-33
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• 2006

This paper proposed device-independent color matching algorithm based on the 3D RGB lookup table (LUT) between mobile camera and mobile LCD (Liquid Crystal Display) to improve the color-fidelity. Proposed algorithm is composed of thee steps, which is device characterization, gamut mapping, 3D RGB-LUT design. First, the characterization of mobile LCD is executed using the sigmoidal function, different from conventional method such as GOG (Gain Offset Gamma) and S-curve modeling, based on the observation of electro-optical transfer function of mobile LCD. Next, mobile camera characterization is conducted by fitting the digital value of GretagColor chart captured under the daylight environment (D65) and tristimulus values (CIELAB) using the polynomial regression. However, the CIELAB values estimated by polynomial regression exceed the maximum boundary of the CIELAB color space. Therefore, these values are corrected by linear compression of the lightness and chroma. Finally, gamut mapping is used to overcome the gamut difference between mobile camera and moible LCD. To implement the real-time processing, 3D RGB-LUT is designed based on the 3D RGB-LUT and its performance is evaluated and compared with conventional method.

• Journal of Bio-Environment Control
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• 제14권1호
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• pp.15-21
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• 2005

This experiment was carried out to investigate the effect of ridge direction (south-north) on temperature and light intensity on early growth of oriental melon under east-west oriented vinyl house cultivation. The air-temperature of minimum between north and south-ridge in the tunnel of vinyl house was $12.5^[\circ}C\;and\;11.3^{\circ}C$ and that of maximum between north and south-ridge was $36.7^{\circ}C\;and\;34.7^{\circ}C$, respectively. The minimum and maximum air-temperature of north-ridge in the tunnel of vinyl house on Feb. 15 were $12^{\circ}C\;and\;2.0^{\circ}C$ higher than those of south-ridge, respectively. The intensity of daylight between south and north-ridge was similar during 9:00-10:30 a.m., that of south-ridge was higher than north-ridge during 10:30-11:30 a.m. and that of north-ridge was higher than south-ridge during 11:30-17:30. The plant growth after 55 days of planting on the north-ridge was prominent cultivation compared to south-ridge. The female flowering and first harvesting day were earlier in north-ridge than in south ridge. The marketable fruits rate and yields (kg/10a) were $6.7\%$ and 218kg higher in north-ridge cultivation than south-ridge, respectively. Differences of marketable fruit rate and yield (kg/10a) in each ridge were significant.

• 한국태양에너지학회:학술대회논문집
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• 한국태양에너지학회 2012년도 춘계학술발표대회 논문집
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• pp.140-145
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• 2012

The purpose of this study is to understand the change of impression by comparing the uniformity lighting with the compound lighting. In previous study, we proposed a light controlling method to harmonize daylight from a window and artificial lights from a ceiling and obtained the results to support our method. We referred this method as the Adjusted Compound-Lighting Model (AC Model). The model claims that lighting in a room with a side-window are perceived as harmonious when the overall light distribution in the room is well approximated by a compound of lights from the window and the entire ceiling. The experiment is carried out with the scaled-models and mock-up spaces that were supposed to be an office space. One is lit by the uniform lighting and the other by the compound lighting in each experimental space. In order to present varying illuminance distributions, thetwo variables were used in this study. One was the ratio of thelight from the window and ceiling in the standard and evaluation box. The standard box was provided by the ratio of 20% to 40% from the window and 80% to 60% from the ceiling as two lighting ratio patterns [Wu20(=Cu80) and Wu40(=Cu60)]. And the evaluation box was provided by the light ratio of 0% to 40 from the window and 100% to 60% from the ceiling [Wcp0(=Ccp100), Wcp20(=Ccp80) and Wcp40(=Ccp60)]. The other variable was themean illuminance level in both boxes. Two level of mean illuminance (700lx and 300lx) were used in this experiment. Each lighting condition was established at equal horizontal mean illuminance level, held constant near 700lx or 300lx in both boxes. Both of them were similar in the shape of distribution when there were same ratios of lights from the window and the ceiling. Subjects were asked to evaluate the point of difference by semantic differential rating on their overall impression after comparing with two rooms. The results showed that the impressions of compound lighting were more positive score than that of uniformity lighting on the items of 'dim-bright', 'dislike-like', 'artificial-natural' and 'closed-open', and that there was no significant difference in impressions between two spaces on other items.

• Proceedings of the Korean Society of Crop Science Conference
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.229-229
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• 2017

This research study was conducted to analyze the characteristics of different rice cultivars in abnormal temperature conditions (low temperature) for ripening period abnormalities, and to investigate the physiological causes behind the abnormalities. Four Korean high quality japonica-type rice cultivars, Jinbu (JB), Junamjosaeng (JJ), Geumyoung (GY), Hwawang (HW) were used in the experiment. The following day after flowering, they were then moved into two phytotrons under natural daylight with 65% RH but controlled at different temperatures - one at $19/29^{\circ}C$ (night/day) and the other at $13/23^{\circ}C$ as the low - temperature study on ripening. For the cultivars at $13/23^{\circ}C$ (low temperature study), JB and JJ had a ripening rate of 93% which is similar to the ripening rates of cultivars at $19/29^{\circ}C$ at 45 days after heading (DAH). In contrast, GY and HW recorded lower ripening rates of 86% and 57% respectively. However, when the cultivars at $13/23^{\circ}C$ were harvested at 61 DAH (when the accumulated temperature reached $1100^{\circ}C$), the difference in ripening rates compared to the 4 cultivars of $19/29^{\circ}C$ harvested at 45 DAH was not obvious (JB 94%, JJ 97%, GY 97%, HW 88%). Starch content showed little difference among the 4 cultivars at different temperature conditions while amylose content was higher for cultivars at $13/23^{\circ}C$ compared to those at $19/29^{\circ}C$. In addition, the enzyme activities of starch biosynthesis were about 5~10 days slower in cultivars at $13/23^{\circ}C$ compared to cultivars at $19/29^{\circ}C$. The grain-filling rate showed highly significant correlations with the enzyme activities of Sucrose synthase ($R^2=0.70^{***}$), ADP glucose pyrophosphorylase ($R^2=0.63^{***}$), UDP glucose pyrophosphorylase ($R^2=0.36^{***}$), Starch synthase ($R^2=0.51^{***}$), and Starch branching enzyme ($R^2=0.59^{***}$). Among the enzymes, Sucrose synthase activity had the highest correlation coefficient with grain-filling rate. In conclusion, the activity of enzymes such as Sucrose synthase, UDP glucose pyrophosphorylase, ADP glucose pyrophosphorylase, Starch synthase, Starch branching enzyme in starch biosynthesis is proven to be highly related to the grain filling process. Notably, the decrease in the activity of Sucrose synthase and Starch branching enzyme and the late increase in ADP glucose pyrophosphorylase activity at low temperature in the ripening stage are considered to be disadvantageous as they delay ripening and increased amylose content.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• 제34권3호
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• pp.259-273
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• 1998

The hydroacoustic surveys to provide the essential information for the assessment, management and utilization of fishery resources in the southern waters of Korea were carried out during five research cruises between October 1996 and October 1997 by the training ship KAYA of Pukyong National University. These hydroacoustic investigations were designed to obtain more precise estimates of the geographic distribution, absolute abundance and biological characteristics of the fishery resources, and the vertically integrated densities of fish in terms of volume backscattering strength(SV) by survey region and depth bins, such as the entire water column and the 0~ 10 m from bottom fraction, were measured separately. Hydroacoustic data were collected by using a Simrad EK 500 Scientific echo sounder operating at two frequencies of 38kHz and 120kHz and the data stored in field were later processed on a HP PC using a Simrad EP 500 echo integration and target strength analysis system. The biological compositions of echo signal were identified and sampled using a demersal trawl during daylight hours. The mean target strength to scale the echo integration data for hydroacoustic surveys was derived from the relationship between the SV and the weight of trawl catch per unit volume of the water column sampled by demersal trawls. The results obtained can be summarized as follow : 1. The mean volume backscattering strength for the entire water column in the southern waters of Korea between 1996 and 1997 were -67.2 dB and -70.9 dB at two frequencies of 38 kHz and 120 kHz , respectively, and for the bottom layer of the 0-10 m from bottom friction were -68.8 dB, -70.2 dB, respectively. That is, the volume backscattering strength for the entire water column at low frequency was higher than that at high frequency. 2. The relationship between the mean backscattering strength (〈SV〉, dB) for the depth strata of trawl hauls and the weight (C, kg/m3) per cubic meter of the catch sampled by bottom trawling in the southern waters of Korea in January and July 1997 were expressed by the following equations: 38 kHz : 〈SV〉= -28.2 + 10 log(C), 120 kHz : 〈SV〉= -32.4 + 10 log(C). The mean weight -normalized target strengths derived from these equitions were -28.2 dB/ kg, -32.4 dB/ kg at 38 kHz and 120 kHz , respectively. That is, the mean weight -normalized target strength at 38 kHz was 4.2 dB higher than that at 120 kHz. 3. The distribution density of fish in terms of biomass per unit volume in the southern waters of Korea were estimated to be 125.9 $\times$ 10-6 kg/m3 and 141.3 $\times$ 10-6 kg/m3 at 38 kHz and 120 kHz , respectively.