• Journal of Environmental Science International
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• v.25 no.8
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• pp.1143-1153
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• 2016

Plant biomass, photosystem II (PSII) photochemical activity, photosynthetic function, and zinc (Zn) accumulation were investigated in a sorghum-sudangrass hybrid (Sorghum bicolor ${\times}$ S. sudanense) exposed to various Zn concentrations to determine the elimination capacity of Zn from soils. Plant growth and biomass of the sorghum-sudangrass hybrid decreased with increasing Zn concentration. Symptoms of Zn toxicity, i.e., withering and discoloration of old leaves, were found at Zn concentrations over 800 ppm. PSII photochemical activity, as indicated by the values of $F_v/F_m$ and $F_v/F_o$, decreased significantly three days after exposure to Zn concentrations of 800 ppm or more. Photosynthetic $CO_2$ fixation rate (A) was high between Zn concentrations of 100-200 ppm ($22.5{\mu}mol$ $CO_2{\cdot}m^{-2}{\cdot}s^{-1}$), but it declined as Zn concentration increased. At Zn concentrations of 800 and 1600 ppm, A was 14.1 and $1.8{\mu}mol$ $CO_2{\cdot}m^{-2}{\cdot}s^{-1}$, respectively. The patterns of stomatal conductance ($g_s$), transpiration rate (E), and water use efficiency (WUE) were all similar to that of photosynthetic $CO_2$ fixation rate, except for dark respiration ($R_d$), which showed an opposite pattern. Zn was accumulated in both above- and below-ground parts of plants, but was more in the below-ground parts. Magnesium (Mg) and iron (Fe) concentrations were significantly low in the leaves of plants, and symptoms of Mg or Fe deficiency, such as a decrease in the SPAD value, were found when plants were treated with Zn concentrations above 800 ppm. These results suggest that the sorghum-sudangrass hybrid is able to accumulate Zn to high level in plant body and eliminate it with its rapid growth and high biomass yield.

• The Plant Pathology Journal
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• v.16 no.5
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• pp.257-263
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• 2000

Infection with rice blast fungus (Magnaporthe grisea) significantly reduced foliar net photosynthesis (A) of rice cultivars: Ilpoom, Hwasung, and Choochung in greenhouse experiments. By measuring the amount of diseased leaf area with a computer image analysis system, the relation between disease severity (DS) and net photosynthetic rate was curvilinearly correlated (r=0.679). Diseased leaves with 35% blast symptom can be predicted to have a 50% reduction of photosynthesis. The disease severity was linearly correlated (r=0.478) with total chlorophyll (chlorophyll a and chlorophyll b) per unit leaf area(TC). Light use efficiency was reduced by the fungal infection according to the light response curves. However, dark respiration (Rd) did not change after the fungal infection (p=0.526). Since the percent of reduction in photosynthesis greatly exceeded the percent of leaf area covered by blast lesions, loss of photosynthetic tissue on an area basis could not by itself account for the reduced photosynthesis. Quantitative photosynthetic reduction can be partially explained by decreasing TC, but cannot be explained by decreasing Rd. By photosynthesis (A)-internal CO$_2$ concentration (C$_i$ curve analysis, it was suggested that the fungal infection reduced ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity, ribulose-1,5-bisphosphate (RuBP) regeneration, and inorganic phosphate regeneration. Thus, the reduction of photosynthesis by blast infection was associated with decreased TC and biochemical capacity, which comprises all carbon metabolism after CO$_2$ enters through the stomata.

• Korean Journal of Agricultural and Forest Meteorology
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• v.14 no.2
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• pp.79-89
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• 2012

This study was conducted to investigate the chlorophyll contents, photosynthetic characteristics, chlorophyll fluorescence, and growth performance of Parasenecio firmus under changing light environment. Parasenecio firmus was grown under non-treated (full sunlight) and three different shading conditions (88~93%, 65~75% and 45%~55% of full sunlight) for the experiment. Total chlorophyll content, photochemical efficiency (Fv/Fm), T/R ratio, specific leaf area (SLA), leaf area ratio (LAR), and leaf weight ratio (LWR) were increased with increasing shading level, but decreased dark respiration. Therefore, light absorption and light utilization efficiency were improved under the low intensity light. Plants under 65~75% of full sunlight had best maximum photosynthetic rate and net apparent quantum yield in May. On the other hand, the non-treated plants had lower maximum photosynthetic rate, photochemical efficiency, and chlorophyll content than the treated ones. Parasenecio firmus considered to be a sciophyte, is fairly sensitive to high intensity light. If 88-93% of full sunlight lasts for a long period, photosynthetic capacity will be sharply decreased, though limiting light. These results suggest that growth of Parasenecio firmus adapted to 65~75% of full sunlight.

• Journal of Ginseng Research
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• v.34 no.3
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• pp.175-182
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• 2010

North American ginseng seedlings (Panax quinquefolius L.) were grown in pots in heated greenhouses, in a cool greenhouse, or in the field, in 11 experiments at various times over 16 years. Crop establishment, dry matter partitioning, photosynthesis, radiation use efficiency and carbon budget were measured and/or calculated in some years. Once the seedling canopy, of about $20\;cm^2$ per seedling, and a leaf area index of 0.37, was established, about 40 days after germination, full canopy display lasted about 87 days. Only 16.6% of the incoming solar radiation was intercepted by the crop, the remainder falling on the mulched soil surface. Total and root dry matter accumulations in the cool greenhouse and in the field were about double that in the heated greenhouses. Partitioning of dry matter to roots (economic yield or harvest index) in the cool greenhouse and in the field was 73% whereas it was 62.5% in the heated greenhouses. The relationship between root dry matter and radiation interception during the full canopy period was linear with growth efficiencies of $2.92\;mg\;MJ^{-1}$ at 4.8% of incoming radiation and $0.30\;mg\;MJ^{-1}$ at 68% of incoming radiation. A photosynthetic rate of $0.39\;g\;m^{-2}\;h^{-1}$ was attained at light saturation of about $150\;{\mu}mol\;m^{-2}\;s^{-1}$ (7.5% of full sunlight); dark respiration was $0.03\;g\;m^{-2}\;h^{-1}$, about 8.5% of maximum assimilation rate. Estimates of dry matter accumulation by growth analysis and by $CO_2$ uptake were similar, 6.21 vs. 7.62 mg $CO_2$, despite several assumptions in $CO_2$ uptake calculations.

• Horticultural Science & Technology
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• v.32 no.3
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• pp.318-329
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• 2014

In order to gain insight into the physiological responses of plants to high temperature stress, the effects of temperature on Chinese cabbage (Brassica campestris subsp. napus var. pekinensis cv. Detong) were investigated through analyses of photosynthesis and chlorophyll fluorescence under 3 different temperatures in the temperature gradient tunnel. Growth (leaf length and number of leaves) during the rosette stage was greater at ambient $+4^{\circ}C$ and ambient $+7^{\circ}C$ temperatures than at ambient temperature. Photosynthetic $CO_2$ fixation rates of Chinese cabbage grown under the different temperatures did not differ significantly. However, dark respiration rate was significantly higher in the cabbage that developed under ambient temperature relative to elevated temperature. Furthermore, elevated growth temperature increased transpiration rate and stomatal conductance resulting in an overall decrease of water use efficiency. The chlorophyll a fluorescence transient was also considerably affected by high temperature stress; the fluorescence yield $F_J$, $F_I$, and $F_P$ decreased considerably at ambient $+4^{\circ}C$ and ambient $+7^{\circ}C$ temperatures, with induction of $F_K$ and decrease of $F_V/F_O$. The values of RC/CS, ABS/CS, TRo/CS, and ETo/CS decreased considerably, while DIo/CS increased with increased growth temperature. The symptoms of soft-rot disease were observed in the inner part of the cabbage heads after 7, 9, and/or 10 weeks of cultivation at ambient $+4^{\circ}C$ and ambient $+7^{\circ}C$ temperatures, but not in the cabbage heads growing at ambient temperature. These results show that Chinese cabbage could be negatively affected by high temperature under a future climate change scenario. Therefore, to maintain the high productivity and quality of Chinese cabbage, it may be necessary to develop new high temperature tolerant cultivars or to markedly improve cropping systems. In addition, it would be possible to use the non-invasive fluorescence parameters $F_O$, $F_V/F_M$, and $F_V/F_O$, as well as $F_K$, $M_O$, $S_M$, RC/CS, ETo/CS, $PI_{abs}$, and $SFI_{abs}$ (which were selected in this study), to quantitatively determine the physiological status of plants in response to high temperature stresses.

• Korean Journal of Medicinal Crop Science
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• v.3 no.3
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• pp.181-186
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• 1995

This study was carried out to know the effect of temperature, light intensity and $CO_2$ con­centration on photosynthesis and respiration in Wasabi (Wasabia japonica Matsum). The optimum temperature for photosynthesis in Wasabi was $17{\sim}20^{\circ}C$ and dark respiration rate was increased with the increasing of tem­perature from, $15 ^{\circ}C\;to\;30^{\circ}C$. Light compensation point was $6.0\;{\mu}E\;m^{-2}s^{-1}$ in Wasabi and $36.7\;{\mu}E\;m^{-2}s^{-1}$ in Corn, and light saturation point was $600{\mu}E\;m^{-2}s^{-1}$, similar in Wasabi and Corn. $CO_2$, compensation point was 67.3ppm in Wasabi and 11.6 ppm in Corn. Photorespiration rate in Wasabi leaf at $l000{\mu}E\;m^{-2}s^{-1}$ light intensity was 3.3 mg$CO_2$, $dm^{-2}hr^{-1}$, and then was gradually decreased as light intensity decreased. Stomatal frequency was about $76\;mm^{-2}$ on the adaxial surface and $623\;mm^{-2}$ on the abaxial surface, and the size of stomata was about 1$12{\mu}m$ on the adaxial surface and $17{\mu}m$ on the abaxial surface of the leaf.

• Korean Journal of Agricultural and Forest Meteorology
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• v.18 no.4
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• pp.357-365
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• 2016

The effects of elevated atmospheric $CO_2$ on photosynthesis and growth of Chinese cabbage (Brassica campestris subsp. napus var. pekinensis) were investigated to predict productivity in highland cropping in an environment where $CO_2$ levels are increasing. Vegetative growth, based on fresh weight of the aerial part, and leaf characteristics (number, area, length, and width) of Chinese cabbage grown for 5 weeks, increased significantly under elevated $CO_2$ ($800{\mu}mol{\cdot}mol^{-1}$) compared to ambient $CO_2$ ($400{\mu}mol{\cdot}mol^{-1}$). The photosynthetic rate (A), stomatal conductance ($g_s$), and water use efficiency (WUE) increased, although the transpiration rate (E) decreased, under elevated atmospheric $CO_2$. The photosynthetic light-response parameters, the maximum photosynthetic rate ($A_{max}$) and apparent quantum yield (${\varphi}$), were higher at elevated $CO_2$ than at ambient $CO_2$, while the light compensation point ($Q_{comp}$) was lower at elevated $CO_2$. In particular, the maximum photosynthetic rate ($A_{max}$) was higher at elevated $CO_2$ by 2.2-fold than at ambient $CO_2$. However, the photosynthetic $CO_2$-response parameters such as light respiration rate ($R_p$), maximum Rubisco carboxylation efficiency ($V_{cmax}$), and $CO_2$ compensation point (CCP) were less responsive to elevated $CO_2$ relative to the light-response parameters. The photochemical efficiency parameters ($F_v/F_m$, $F_v/F_o$) of PSII were not significantly affected by elevated $CO_2$, suggesting that elevated atmospheric $CO_2$ will not reduce the photosynthetic efficiency of Chinese cabbage in highland cropping. The optimal temperature for photosynthesis shifted significantly by about $2^{\circ}C$ under elevated $CO_2$. Above the optimal temperature, the photosynthetic rate (A) decreased and the dark respiration rate ($R_d$) increased as the temperature increased. These findings indicate that future increases in $CO_2$ will favor the growth of Chinese cabbage on highland cropping, and its productivity will increase due to the increase in photosynthetic affinity for light rather than $CO_2$.

• ALGAE
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• v.31 no.1
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• pp.49-59
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• 2016

Ulva pertusa, a common bloom-forming green alga, was used as a model system to examine the effects of elevated carbon dioxide (CO₂) and temperature on growth and photosynthetic performance. To do this, U. pertusa was grown under four temperature and CO₂ conditions; ambient CO₂ (400 μatm) and temperature (16℃) (i.e., present), elevated temperature only (19℃) (ET; i.e., warming), elevated CO₂ only (1,000 μatm) (EC; i.e., acidification), and elevated temperature and CO₂ (ET and EC; i.e., greenhouse), and its steady state photosynthetic performance evaluated. Maximum gross photosynthetic rates (GP_max) were highest under EC conditions and lowest under ET conditions. Further, ET conditions resulted in decreased rate of dark respiration (R_d), but growth of U. pertusa was higher under ET conditions than under ambient temperature conditions. In order to evaluate external carbonic anhydrase (eCA) activity, photosynthesis was measured at 70 μmol photons m⁻² s⁻¹ in the presence or absence of the eCA inhibitor acetazolamide (AZ), which inhibited photosynthetic rates in all treatments, indicating eCA activity. However, while AZ reduced U. pertusa photosynthesis in all treatments, this reduction was lower under ambient CO₂ conditions (both present and warming) compared to EC conditions (both acidification and greenhouse). Moreover, Chlorophyll a and glucose contents in U. pertusa tissues declined under ET conditions (both warming and greenhouse) in conjunction with reduced GP_max and R_d. Overall, our results indicate that the interaction of EC and ET would offset each other’s impacts on photosynthesis and biochemical composition as related to carbon balance of U. pertusa.

• Journal of Environmental Science International
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• v.29 no.10
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• pp.989-996
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• 2020

This study was conducted to determine the effect of low temperature and low radiation conditions on the yield and quality of hot pepper at an early growth stage in Korea. In plastic greenhouses, low temperature, low temperature with covered shading treatments were set 17 to 42 days after transplanting. The pepper growing degree days decreased by 5.5% due to the low temperature during the treatment period. Radiation decreased by 74.7% due to the covered shading. After commencing treatments, pepper plant growth decreased with low temperature and low radiation. Analysis of the yield showed that the first harvest was delayed by low radiation. The cumulative yields of 119 days after transplanting were 1,956, 2,171, and 2,018 g/㎡ for control, low temperature, and low temperature with low radiation respectively. Capsaicin and dihydrocapsaicin concentrations in pepper fruit decreased with low temperature and low radiation. To investigate the photosynthetic characteristics according to the treatment, the carbon dioxide reaction curve was analyzed using the biochemical model of photosynthesis. Results showed that the maximum photosynthetic rate, V_cmax (maximum carboxylation rate), J (electric transportation rate), and TPU (triose phosphate utilization) decreased at low temperatures; the maximum photosynthetic rate, J, and g_m (dark respiration rate) were reduced by shading. These results indicate that low temperature and low radiation can retard early growth, yield, and quality, but these can also be recovered 119 days after planting. Based on the results, the yield and quality of pepper can recover from abiotic stresses with proper cultivation.

• Korean Journal of Agricultural and Forest Meteorology
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• v.17 no.1
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• pp.75-84
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• 2015

This study was conducted to investigate the effects of high temperature and drought on growth performance, photosynthetic parameters and photosynthetic pigment contents of Liriodendron tulipifera L. seedlings. The seedlings were grown in controlled-environment growth chambers with combinations of four temperature ($-3^{\circ}C$, $0^{\circ}C$, $+3^{\circ}C$, $+6^{\circ}C$; based on the monthly average for 30 years in Korea) and two water status (control, drought). Temperature rise increased growth, total dry weight and leaf area in all water status. Also photosynthetic rate, dark respiration, stomatal conductance and transpiration rate increased with increasing temperature. In contrast, growth and photosynthetic parameters of L. tulipifera seedlings were lower in $-3^{\circ}C$ than $0^{\circ}C$. But temperature rise decreased water use efficiency in all water status. Temperature rise increased photosynthetic pigment contents of leaf. Also chlorophyll a/b ratio increased with increasing temperature. In conclusion, the elevated temperature lead to causes growth increase through the increase of energy production by higher photosynthetic rate during a growth period of L. tulipifera seedlings.