• Composites Research
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• 제15권1호
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• pp.53-60
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• 2002

본 연구에서는 내열 특성과 구조적인 특성을 갖는 탄소섬유/페놀릭 복합재 내열튜브를 필라멘트와인딩 공법에 의해 제작하고 이들의 구조적인 성능을 평가하였다. 이를 위해 내열튜브의 제작방법을 소개하고 황산용해법을 적용하여 내열튜브에서의 섬유체적비와 기공함유율을 측정하였다. 시편폭을 달리한 인장시편의 기계적 특성을 평가함으로써 강화섬유의 연속성을 나타낼 수 있는 시편형상을 제시하였으며 공정변수를 달리한 내열튜브에서 채취된 인장시편의 기계적 특성을 평가함으로써 내열튜브의 제작을 위한 적절한 공정조건을 결정하였다. 그리고 보증시험을 통해 수집한 음향신호의 분석을 통해 내열튜브에서의 균열진전과 파손양상을 조사하였다. 마지막으로 내열튜브 자체에 대해 보증시험과 파열시험을 수행함으로써 내열튜브 자체의 구조적인 신뢰성을 확인하였다.

• 생약학회지
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• 제36권1호통권140호
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• pp.56-59
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• 2005

Urease plays an important role in the urea metabolism and the effect of urease activity on human and environment is enormous. For instance, urease acts as a virulence factor of the urinary and gastrointestinal tracts infections in human and animal, being involved in kidney stone formation, catheter encrusatation, pyelonephritis, ammonia encephalopathy, hepatic coma, and urinary tract infections. Widespread urease activity in soil induces a plant damage due to ammonia toxicity and pH increase. Therefore, urease activity regulation through urease inhibitors would lead to an enhanced efficiency of urea nitrogen uptake in plants and to the improved therapeutic strategies for ureolytic bacterial infections. To search for new inhibitory compounds on urease activity from herbs, MeOH extracts of herbs were screened. Among of them, the MeOH extracts of Areca catechu exhibited an excellent inhibitory effect on urease activity. Two compounds were isolated from the ethyl acetate fraction by the activity guided fractionation. Their chemical structures were identified as (+)-catechin(compound I) and allantoin(compound II) by spectroscopic evidence, respectively. Compound I showed a stronger inhibitory effect on urease activity than compound II.

• Journal of Ginseng Research
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• 제41권2호
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• pp.180-187
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• 2017

Background: The incidence of halitosis has a prevalence of 22-50% throughout the world and is generally caused by anaerobic oral microorganisms, such as Fusobacterium nucleatum, Clostridium perfringens, and Porphyromonas gingivalis. Previous investigations on the structure-activity relationships of ginsenosides have led to contrasting results. Particularly, the antibacterial activity of less polar ginsenosides against halitosis-related bacteria has not been reported. Methods: Crude saponins extracted from the Panax quinquefolius leaf-stem (AGS) were treated at $130^{\circ}C$ for 3 h to obtain heat-transformed saponins (HTS). Five ginsenoside-enriched fractions (HTS-1, HTS-2, HTS-3, HTS-4, and HTS-5) and less polar ginsenosides were separated by HP-20 resin absorption and HPLC, and the antimicrobial activity and mechanism were investigated. Results: HPLC with diode-array detection analysis revealed that heat treatment induced an extensive conversion of polar ginsenosides (-Rg1/Re, -Rc, -Rb2, and -Rd) to less polar compounds (-Rg2, -Rg3, -Rg6, -F4, -Rg5, and -Rk1). The antimicrobial assays showed that HTS, HTS-3, and HTS-4 were effective at inhibiting the growth of F. nucleatum, C. perfringens, and P. gingivalis. Ginsenosides-Rg5 showed the best antimicrobial activity against the three bacteria, with the lowest values of minimum inhibitory concentration and minimum bactericidal concentration. One major reason for this result is that less polar ginsenosides can more easily damage membrane integrity. Conclusion: The results indicated that the less polar ginsenoside-enriched fraction from heat transformation can be used as an antibacterial agent to control halitosis.

• Journal of Acupuncture Research
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• 제18권2호
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• pp.111-122
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• 2001

Objectives ; This study was undertaken to determine if Salviae Radix herb-acupuncture (SRA) exerts protective effect against alterations in membrane transport function in rabbits with mercury chloride (Hg)-induced acute renal failure. Methods and Results ; The administration of Hg at a subcutaneous single dose of 10mg/kg caused a reduction in GFR to 9.4% of the basal value and an increase in fractional Na+ excretion to 10-fold, indicating generation of acute renal failure. When animals were acupunctured with $0.5m{\ell}$ of SRA extract (0.1%) in both sides of Shinsu(BL23) for 7 days prod to Hg administration, such changes were significantly attenuated. The fractional excretion of glucose and phosphate was increased to approximately 132-fold and 7-fold, respectively, in rabbits treated with Hg alone, but the fractional excretion of glucose was increased to 26-fold and that of phosphate was not different from the basal value in SRA-pretreated rabbits. Uptakes of glucose and phosphate in purified isolated brush-border membrane and $Na^+-K^+$-ATPase activity in microsomal fraction were inhibited in rabbits treated with Hg alone, suggesting that impairment in proximal reabsorption of glucose and phosphate is resulted from a direct damage of membrane transport carriers and disruption of the normal $Na^+$ gradient. Conclusions ; Such changes were prevented by SRA. Uptakes of organic ions, PAH and TEA, in renal cortical slices were inhibited by the administration of Hg, which was prevented by SRA. Pretreatment of an antioxidant DPPD attenuated the increase in the fractional excretion of glucose and phosphate induced by the administration of Hg.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2008년도 춘계 학술발표회 제20권1호
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• pp.925-928
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• 2008

최근 내진부재 및 기존 구조물의 내진보강요소의 손상제어(Damage tolerance)성능을 충족할 수 있는 변형경화형 시멘트 복합체(Strain-hardening cement composites, SHCC)의 개발 및 활용 연구가 진행 중이며, 하이브리드화에 따른 경제성 및 성능향상 가능성도 보고되고 있다. 그러나 이러한 우수한 성능을 갖는 SHCC 재료가 실구조물의 보수/보강재 및 내진보강부재에 적용되기 위해서는 우수한 인장성능 발현뿐만 아니라, 보강섬유의 단가를 고려한 경제적 효과(Economical efficiency) 및 시공성 (Workability)이 요구된다. 따라서 본 연구에서는 SHCC를 내진부재 및 보강재료로써 적용하기 위한 연구의 일환으로 합성섬유를 하이브리드하여 혼입시 휨 및 인장강도, 변형능력 등 거동특성을 분석함으로써 각 보강섬유의 인장강도 탄성계수 등 기계적 특성과 혼입율에 따른 재료성능과의 상관관계를 비교 분석하여 평가하고자 한다. 또한 물시멘트비를 변수로 하여 시멘트 복합체의 강도특성과 보강섬유의 부착특성 및 균열제어성능을 규명하고자 한다. 이러한 결과를 근거로 향후 SHCC 재료의 실구조물 적용시 요구성능 및 경제성을 고려한 재료배합에 관한 기초자료를 제시하고자 한다.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2008년도 춘계 학술발표회 제20권1호
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• pp.177-180
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• 2008

SHCC는 섬유와 시멘트 메트릭스 계면의 부착작용으로 인해 높은 에너지 흡수능력을 보여준다. 서로 다른 종류의 섬유로 보강된 SHCC는 혼입되는 섬유자체가 가지는 재료적 특성에 의해 서로 다른 특성을 나타내기 때문에 시멘트 메트릭스와 혼입되는 섬유의 상호작용에 의한 손상진전에 따른 미세적 파괴 메카니즘에 대한 평가가 필요할 것으로 판단된다. 이에 본 연구에서는 AE기법을 사용하여 단독섬유와 하이브리드 섬유를 혼입한 시멘트 복합체의 직접인장특성을 평가하고자 하였다. 이러한 목적으로 본 실험에서는 PET2.0%, PET1.5%+PE0.5%, PET1.5%+PVA0.5%의 세 종류의 단독섬유 및 하이브리드 섬유를 혼입하여 실험을 실시하였으며, 실험에서 나타난 AE신호와 직접인장실험결과를 상호비교 분석하였다. 직접인장실험결과, 같은 혼입율에서 PET만을 단독혼입한 시험체에 비해 PET-PE시험체에서 최대 강도에서 약 2.7배 높게 나타났으며. 손상진전에 따른 AE신호결과, 혼입되는 섬유의 재료적 특성에 따라 AE이벤트수와 누적에너지에서 상이한 특성을 나타내었다.

• 약학회지
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• 제48권1호
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• pp.34-40
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• 2004

Hovenia dulcis which is distributed in Korea, China and Japan is known to show hepatoprotctive effect and reduce the acute alcohol toxicity. In this study, the hepatoprotective effect against the chemically induced experimental liver injury models and lowering effect of blood alcohol level in animal models acutely administered alcohol by the peduncle extracts of Hovenia dulcis var. koreana were investigated. HdfHW-1, and HdfM-1 which are the extracts of fruit peduncles and young branches with hot water or 70％ methanol and followed with 100％ methanol, were significantly reduced the $CCl_4$ or D-galactosamine/LPS induced damage in sliced liver. The hot water or methanol extracts of fruit peduncle protected dose-dependently against $CCl_4$ induced toxicity in primary hepatocyte culture and particularly, the amount of LDH release was reduced to the control level at 500 $\mu\textrm{g}$/ml of hot water extracts. HdfHW-1 also decreased the hepatotoxicity induced by $CCl_4$ in rats. The active components of HdfHW-1 seemed to be high molecular weights because 0.2 M NaCl HdfHW-1 fraction was the most effective among NaCl fractions of HdfHW-1 eluted with various concentrations of NaCl on DEAE 650C column chromatography. HdfM and HdfHW were significantly reduced the levels of blood alcohol in rats and mice administered 40％ alcohol. These results indicated that the hot water or methanol extracts of fruit peduncle of Hovenia dulcis var. koreana have hepatoprotective effect and may be reduce alcohol toxicity.

• Biomolecules & Therapeutics
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• 제14권4호
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• pp.240-245
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• 2006

The primary use for glycidol is as a stabilizer in the manufacture of vinylpolymers, however, it is also used as an intermediate in the production of pharmaceuticals, as an additives for oil and synthetic hydraulic fluids, and as a diluting agent is same epoxy resins. In this study, we have carried out in vitro genetic toxicity test of glycidol and microarray analysis of differentially expressed genes in response to glycidol. The result of Ames test showed mutations with glycidol treatment in base substitution strain TA1535 both with and without exogenous metabolic activation. Likewise, glycidol showed mutations in frame shift TA98 both with and without exogenous metabolic activation. The result of COMET assay in L5178Y cells with glycidol treatment showed DNA damage both with and without exogenous metabolic activation. Glycidol increased micronuclei in CHO cells both with and without exogenous metabolic activation. 150 Genes were selected as differentially expressed genes in response to glycidol by microarray analysis and these genes would be candidate biomarkers of genetic toxic action of glycidol.

• 한국전통의학지
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• 제15권1호
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• pp.97-105
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• 2006

To study on antioxidant effects in the liver of 40-week-old mouse, the sample were orally pretreated 5mg/kg/day for 5 days with red ginseng saponin components(total saponin, protopanaxadiol saponin, protopanaxatriol saponin, ginsenoside-Rd, ginsenoside-Re, compound-K) for 5 days. The ability of saponin to protect the mouse liver from oxidative damage was examined by determining the activity of superoxide dismutase(SOD), glutathione peroxidase(GPx) and the contents of glutathione, the level of malondialdehyde, The only protopanaxadiol among the ginseng saponin fractions was significantly increased the hepatic SOD activity(p<0.01). The red ginseng saponin induced a slight increase of GPx activity, especially ginsenoside Rd, compound K and protopanaxatriol treatments significantly increased its activity. The content of glutathione was significantly increased by total saponin, protopanaxadiol and ginsenoside Rd(p<0.01), but the oxidized glutathione level was lowered in all the red ginseng saponin. Finally, the level of malondialdehyde was significantly decreased by ginsenoside Rd and protopanaxadiol. In conclusion, protopanaxadiol and ginsenoside Rd among the saponin fraction were especially increased in the activity of hepatic antioxidative enzyme and decreased the lipid peroxidation that was expressed in term of MDA formation. This comprehensive antioxidant effects of red ginseng saponin seems to be by a certain action of saponin other than a direct antioxidant action.

• The Korean Journal of Physiology and Pharmacology
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• 제25권5호
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• pp.439-448
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• 2021

DA-9601 is an extract obtained from Artemisia asiatica, which has been reported to have anti-inflammatory effects on gastrointestinal lesions; however, its possible anti-inflammatory effects on the small intestine have not been studied yet. Therefore, in this study, we investigated the protective effects of DA-9601 against the ACF-induced small intestinal inflammation. Inflammation of the small intestine was confirmed by histological studies and the changes in the CD4⁺ T cell fraction induced by the inflammation-related cytokines, and the inflammatory reactions were analyzed. Multifocal discrete small necrotic ulcers with intervening normal mucosa were frequently observed after treatment with ACF. The expression of IL-6, IL-17, and TNF-α genes was increased in the ACF group; however, it was found to have been significantly decreased in the DA-9601 treated group. In addition, DA-9601 significantly decreased the levels of proinflammatory mediators such as IL-1β, GM-CSF, IFN-γ, and TNF-α; the anti-inflammatory cytokine IL-10, on the other hand, was observed to have increased. It is known that inflammatory mediators related to T cell imbalance and dysfunction continuously activate the inflammatory response, causing chronic tissue damage. The fractions of IFN-γ⁺ Th1 cells, IL-4⁺ Th2 cells, IL-9⁺ Th9 cells, IL-17⁺ Th17 cells, and Foxp3⁺ Treg cells were significantly decreased upon DA-9601 treatment. These data suggest that the inflammatory response induced by ACF is reduced by DA-9601 via lowering of the expression of genes encoding the inflammatory cytokines and the concentration of inflammatory mediators. Furthermore, DA-9601 inhibited the acute inflammatory response mediated by T cells, resulting in an improvement in ACF-induced enteritis.