• 공업화학
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• 제29권2호
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• pp.176-184
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• 2018

본 논문에서는 금전초로부터 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획을 제조하였고 이들의 항산화 활성, 세포 보호 효과 및 성분 분석에 대한 연구를 수행하였다. 항산화능 평가에서 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획의 라디칼 소거 활성($FSC_{50}$)은 각각 146.8, 22.2 및 $27.2{\mu}g/mL$이었고, 총 항산화능($OSC_{50}$)은 29.3, 2.9 및 $4.5{\mu}g/mL$이었다. 에틸아세테이트 분획의 자유라디칼 소거 활성 및 총 항산화능이 가장 크게 나타났다. 또한 $^1O_2$로 유도된 적혈구 광용혈에 대한 세포 보호 효과(${\tau}_{50}$)는 금전초 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획 $5{\mu}g/mL$에서 각각 26.9, 57.5 및 103.9 min을 나타냈다. 특히 아글리콘 분획물의 ${\tau}_{50}$은 (+)-${\alpha}$-tocopherol (37.7 min)보다 훨씬 큰 세포 보호 효과를 나타내었다. UVB로 유도된 세포 손상에서 에틸아세테이트 분획물은 최대 90.1%까지 세포 생존율을 증가시켰다. 과산화수소로 유도된 세포 손상에서도 에틸아세테이트 분획물은 $5-25{\mu}g/mL$에서 농도 의존적으로 세포 보호 효과를 나타내었다. 금전초 에틸아세테이트 분획물의 성분 분석을 위해 TLC, HPLC, UV-vis spectrum, LC-MS로 분석한 결과, quercetin, kaempferol 및 그 배당체가 주성분들로 확인되었다. 결론적으로 금전초가 자외선에 노출된 피부를 보호하는 항산화제로서 작용할 수 있고, 기능성 화장품 원료로 응용 가능성이 있음을 시사한다.

• 한국응용과학기술학회지
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• 제33권4호
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• pp.647-657
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• 2016

본 연구에서는 하고초(Prunella vulgaris L.)의 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획을 대상으로 항산화 활성과 유효성분 분석 연구를 수행하였다. 1,1-Diphenyl-2-picrylhydrazyl (DPPH)를 이용한 자유라디칼 소거활성($FSC_{50}$)은 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획에서 각각 $15.25{\mu}g/mL$, $8.68{\mu}g/mL$ 및 $8.25{\mu}g/mL$이었다. Luminol 발광법을 이용한 $Fe^{3+}-EDTA/H_2O_2$계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 하고초 추출물의 총항산화능($OSC_{50}$)은 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획에서 각각 $4.68{\mu}g/mL$, $1.00{\mu}g/mL$ 및 $1.02{\mu}g/mL$이었다. $^1O_2$로 유도된 적혈구 세포손상에 대한 보호효과는 $1{\sim}25{\mu}g/mL$의 농도에서 농도 의존적으로 증가하였고, 특히 $25{\mu}g/mL$ 농도에서 아글리콘 분획의 ${\tau}_{50}$이 337.9 min으로 높은 세포 보호 활성을 나타내었다. 이는 대표적인 항산화 물질로 알려진 (+)-${\alpha}$-tocopherol (${\tau}_{50}=38.7$ 분)보다도 9배나 큰 세포 보호 효과를 나타냄을 알았다. 실험에 사용된 하고 초 추출물의 에틸아세테이트 분획과 아글리콘 분획에 대하여 TLC와 HPLC를 이용한 성분 분석을 수행하였다. 에틸아세테이트 분획에서는 caffeic acid, rosmarinic acid, quercetin 3-${\beta}$-D-glucoside, rutin, kaempferol-3-O-rutinoside, astragalin (kaempferol-3-O-glucoside)을 확인하였다. 아글리콘 분획에서는 caffeic acid, rosmarinic acid, quercetin 및 kaempferol이 존재함을 확인하였다. 이상의 결과들로부터, 하고초 추출물 및 분획물은 $^1O_2$ 또는 ROS를 소거함으로써 세포막을 보호할 수 있는 천연 항산화제로서 화장품 분야에서 응용이 가능함을 시사하였다.

• 대한화장품학회지
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• 제41권2호
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• pp.181-187
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• 2015

본 연구에서는 명월초 추출물의 메탄올 분획물과 아글리콘 분획물을 제조하였고, 이들에 대한 항산화 활성을 측정하였다. 항산화 활성 측정은 1,1-diphenyl-2-picrylhydrazil (DPPH) radical을 이용한 프리 라디칼 소거활성, 루미놀 발광법을 이용한 활성산소 소거활성(총 항산화능) 및 활성산소($^1O_2$ 등)로 유도된 세포손상에 있어서 세포 보호 효과를 측정하였다. 명월초 추출물의 프리 라디칼 소거활성($FSC_{50}$)은 메탄올 분획이 $90.25{\mu}g/mL$이고, 당을 제거한 아글리콘 분획은 $81.38{\mu}g/mL$를 나타내었다. 총 항산화능($OSC_{50}$)은 메탄올 분획 $16.96{\mu}g/mL$, 아글리콘 분획 $12.30{\mu}g/mL$로, 프리 라디칼 소거활성 및 활성산소 소거활성 모두 아글리콘 분획에서 큰 활성을 나타내었다. $^1O_2$로 유도된 사람 적혈구의 세포손상에 대한 보호 효과는 ${\tau}_{50}$ 값으로 확인하였다. $5{\mu}g/mL$ 농도에서 메탄올 분획 및 아글리콘 분획의 ${\tau}_{50}$이 각각 36.7 및 76.1 min으로, 비교 물질인 (+)-${\alpha}$-tocopherol (35.4 min)과 비교했을 때 아글리콘 분획이 약 2배 더 큰 세포 보호 효과를 나타내었다. 이러한 결과들은 명월초 추출물의 아글리콘 분획물이 항노화 관련 화장품에 있어서 항산화소재로서 응용가능성이 있음을 시사하였다.

• 대한한의학회지
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• 제23권3호
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• pp.145-163
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• 2002

Objectives: The purpose of this investigation was to evaluate the effects of Woohwangcheongsim-won and to study the mechanism for neuronal death protection in hypoxia with Embryonic day 20 (E20) cortical cells of a rat (Sprague Dawley). Methods: E20 cortical cells were dissociated in neurobasal media and grown for 14 days in vitro (DIV). On 14 DIV, Woohwangcheongsim-won was added to the culture media for 24 hrs or 72 hrs. On 17 DIV, cells were given a hypoxic shock and further incubated in normoxia for another three days. On 20 DIV, Woohwangcheongsim-won's effects for neuronal death protection were evaluated by LDH assay, propidium iodide stain and phospho-H2AX immunostain and the mechanisms were studied by Bcl-2, Bak, Bax, caspase family, PKCα, ca1pain I. Results & Conclusions : 1. This study indicated that Woohwangcheongsim-won's effects for neuronal death protection in hypoxia were confirmed by LDH assay, propidium iodide stain and phospho-H2AX immunostain in culture method of Embryonic day 20(E20) cortical neuroblasts. 2. Woohwangcheongsim-won's mechanisms for neuronal death protection in hypoxia are to reduce the membrane damage fraction, to restrain DNA truncate, to restrain inflow of cytochrome c into cellularity caused by Bak diminution, to reduce the caspase cascade intiator caspase-8 and the effector caspase-3, to reduce the calpain I activity and to increase PKCand its activity in the membrane fraction. (J Korean Oriental Moo 2002;23(3):145～163)

• 한국식품영양학회지
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• 제24권4호
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• pp.740-745
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• 2011

Ohmic heating uses electric resistance heat which occurs equally and rapidly inside food when an electrical current is passed throught. In this study, we observed the physical & chemical characteristics changes which occurs in soybean protein during heating denaturation by using ohmic and conventional heating. After the ohmic heating process, we could not find any change of the primary protein structure in the denaturated soy protein samples. However, the rate of imbibed water(RIW) of the ohmic samples was 2 times faster than that of the conventional samples. Also the ANS-surface hydrophobicity was decreased, which is very closely related to RIW. In the differential scanning calorimeter(DSC) analysis result, all 7S soyprotein fraction samples were completely denaturated by ohmic and conventional heating. However, the 11S samples were completely denatured only by ohmic heating. According to the DSC result, we decided that soyprotein was damaged by temperature and electrical current during ohmic heating. The damage of electrical current was a cause of the characteristic changes.

• 대한의생명과학회지
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• 제17권3호
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• pp.203-209
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• 2011

Magnesium (Mg) plays an essential role in physiological and metabolic reactions. Recently, there has been an increased interest in the role of Mg deficiency, particularly the relationship between serum Mg value and inflammatory response. This study was designed to determine the relationship between serum Mg deficiency with inflammatory response, electrolytes and hematological alteration over long-term periods. Sixteen male Sprague-Dawley rats were divided into two groups: control (n=8), and Mg deficiency group (MgD group, n=8). Chow and normal water (tap water) were regularly provided to the control group and Mg-depleted chow and third distilled water were regularly provided for 60 days to the MgD group. Body weights, Serum Mg, $K^+$, inorganic phosphorus (IP) and total iron binding capacity (TIBC) levels in the MgD group were lower than those of the control group (P<0.05). Granulocyte fraction and MCV, RDW and PDW levels were higher, whereas lymphocyte fraction, erythrocyte, hemoglobin and MCHC levels were lower in the MgD group than in the control group (P<0.05). MCP-1 and TNF-${\alpha}$ levels in the MgD group were greater than those of the control group (P<0.05). In conclusion, the results of the present study suggest that Mg deficiency over a long-term period had not altered total leukocyte concentration in the blood, but had detrimental effects, including disturbances of electrolytes balance, disturbance of iron indices, potential anemia and elevation of pro-inflammatory cytokine. However, further studies should be performed to determine the relationship between serum Mg deficiency and major organ damage or alteration.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2010년도 제39회 하계학술대회 초록집
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• pp.203-203
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• 2010

Nano-crystalline Si thin films were deposited on polymer and glass by inductively coupled plasma (ICP) - assisted RF magnetron sputtering at low temperature in an argon and hydrogen atmosphere. Internal ICP coil was installed to increase hydrogen atoms dissociated by the induced magnetic field near the inlet of the working gases. The microstructure of deposited films was investigated with XRD, Raman spectroscopy and TEM. The crystalline volume fraction of the deposited films on polymer was about 70% at magnetron RF power of 600W and ICP RF power of 500W. Crystalline volume fraction was decreased slightly with increasing magnetron RF power due to thermal damage by ion bombardment. The diffraction peak consists of two peaks at $28.18^{\circ}$ and $47.10^{\circ}\;2{\theta}$ at magnetron RF power of 600W and ICP RF power of 500W, which correspond to the (111), (220) planes of crystalline Si, respectively. As magnetron power increase, (220) peak disappeared and a dominant diffraction plane was (111). In case of deposited films on glass, the diffraction peak consists of three peaks, which correspond to the (111), (220) and (311). As the substrate temperature increase, dominant diffraction plane was (220) and the thickness of incubation (amorphous) layer was decreased.

• Journal of Power Electronics
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• 제7권1호
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• pp.64-71
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• 2007

This paper proposes a novel active frequency drift (AFD) method to improve the islanding detection performance with minimum current harmonics. To detect the islanding phenomenon of grid-connected photovoltaic (PV) inverters concerning the safety hazards and possible damage to other electric equipment, anti-islanding methods have been described. The AFD method that uses chopping fraction (cf) enables the islanding detection to drift up (or down) the frequency of the voltage during the islanding situation. However, the performance of the conventional AFD method is inefficient and causes difficulty in designing the appropriate cf value to meet the limit of harmonics. In this paper, the periodic chopping fraction based on a novel AFD method is proposed. This proposed method shows the analytical design value of cf to meet the test procedure of IEEE Std. 929-2000 with power quality and islanding detection time. To verify the validation of the proposed method, the islanding test results are presented. It is confirmed that the proposed method has not only less harmonic distortion but also better performance of islanding detection compared with the conventional AFD method.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2003년도 추계학술대회논문집
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• pp.84-91
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• 2003

Flow-Induced Vibration of steam generator tubes may result in fretting wear damage at the tube-to-support locations. KSNP(Korean Standard Nuclear Power plant) steam generators experienced fretting wear in the upper part of U-bend above the central cavity region of steam generators. This region has conditions susceptible to the flow-induced vibration, such as high flow velocity, high void fraction, and longer unsupported span. To improve its performance, APR1400 steam generator is designed with additional supports in this region to reduce unsupported span and to reduce peak velocity in the central cavity region. In this paper, we examined its performance improvement using ATHOS code. The thermal-hydraulic condition in the region of secondary side of APR1400 steam generator is obtained using the ATHOS3 code. The effective mass for modal analysis is calculated using the void fraction, enthalpy, and operating pressure information from ATHOS3 code result. With the effective mass distribution along the tube, natural frequency and mode shape is obtained using ANSYS code. Finally, stability ratios and real mean squared displacements for selected tubes of the APR1400 steam generator are computed. From these results, the current design of the APR1400 steam generator are examined.

• Journal of Applied Biological Chemistry
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• 제51권4호
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• pp.148-154
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• 2008

Excessive alcohol use causes oxidative stress in the liver, and antioxidant therapy has been an attractive approach for the treatment of ethanol-induced liver damage. The present study examined the hepatoprotective effect of Sasa quelpaertensis Nakai (Korean name, Jeju-Joritdae) in C57BL/6 mice intoxicated with ethanol. Mice were intraperitoneally administered with ethanol alone, or together with test materials three times at 12-h intervals. At 3 h after the last dosing, hepatotoxicity was assessed based on serum activities of aspartate aminotransferase and alanine aminotransferase, and hepatic contents of thiobarbituric acid-reactive substances and glutathione. Sasa quelpaertensis extract mitigated the acute ethanol hepatotoxicity as effectively as silymarin. Its n-butanol fraction was more active than methylene chloride or aqueous fraction. p-Coumaric acid, a major constituent of S. quelpaertensis, was found to effectively prevent the ethanol-induced hepatotoxicity. These data suggest that S. quelpaertensis and p-coumaric acid could be useful for the prevention of liver disease caused by alcohol abuse.