• 한국수정란이식학회지
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• 제5권2호
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• pp.38-44
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• 1990

This study was carried out to produce superior dairy cattle by embryo transfer. Seven dairy cows were superovulated with divided injection of FSH 4Omg for 5 days started on day 9 to 14 of the estrus cycle and injection of PGF$_2$$\alpha$ 45mg on day 4 of FSH injection. Donor cows were flushed to collect embryos on day 7 or 8 of the estrus cycle. Fresh embryos collected were transferred to synchronized dairy recipients or frozen using glycerol 3 step method to he equilibrated. And 35 embryos which were frozen using glycerol 6 step method were imported from U.S.A. After glycerol dilution of frozen embryos was done by reverse density during freezing. frozen-thawed embryos were transferred to synchronized dairy or beef recipients. The results obtained were as follows; 1. Total of 24 embryos were collected from 7 donor cows flushed and transferable embryos were 18 (75.0%). 2. Among 24 embryos. morula, early blastocyst, blastocyst, expanded blastocyst and unfertilized ova were 3 (12.5%), 1 (4.2%), 10 (41.6%), 4 (16.7%) and 6 (25.0%), respectively. 3. Heat inducing rate after 1st and 2nd injections of PGF$_2$$\alpha$ in Holstein and beef cattle was 83.3% and 71.4% and 62.5% and 69.2%, respectively. 4. Among 56 recipients, 23 head were pregnant (41.1%). The pregnancy rate of fresh embryos was 50.0% (1/2 heads) and the pregnancy rate of frozen embryos which were frozen using glycerol 3 step and using glycerol 6 step imported from U.S.A. was 52.6%(l0/19 heads) and 34.3%(12/35 heads), respectively. 5. The pregnancy rate of blastocyst (60.0%) was higher than that of morula (39.0%), early blastocyst (25.0%) and expanded blastocyst (0%). 6. The pregnancy rate of grade I embryos (52.2%) was higher than that of grade 2 (34.6%) and grade 3 (28.6%). 7. The pregnancy rate according to synchrony of recipient with donor was higher in simultaneous recipient (55.0%) and +l2hrs' (53.8%) than -24hrs' (23.5%), -l2hrs' (20.0%) and +24hrs' (0%).

• Asian-Australasian Journal of Animal Sciences
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• 제20권6호
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• pp.842-849
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• 2007

The purpose of the present study was to produce live offspring from in vitro fertilized goat embryos. Oocytes were collected from abattoir ovaries and kept in oocyte collection medium. Oocytes were washed 4-5 times with maturation medium containing medium-199 with 5 ${\mu}g/ml$ FSH, 100 ${\mu}g/ml$ LH, 1 ${\mu}g/ml$ estradiol-$17{\beta}$ 50 ${\mu}g/ml$ gentamycin, 10% inactivated estrus goat serum, and 3% BSA (fatty acid free). Oocytes were placed in 100 ${\mu}l$ drops of maturation medium containing granulosa cell monolayer and incubated in a 5% $CO_2$ incubator at $38.5^{\circ}C$ for 27 h. For capacitation of spermatozoa fresh semen was processed and mixed in 3 ml fertilization TALP medium containing 50 ${\mu}g/ml$ heparin and kept in the above incubator for 2 h. The capacitated spermatozoa were coincubated with matured oocytes for fertilization. Cleaved embryos were separated and cultured in embryo development medium with oviductal cells and 494 embryos were produced. Recipient goats were synchronized with two injections of 15 mg $PGF_{{2}{\alpha}}$/goat 10 days apart. Eighty early stage embryos were transferred into the uterotubal junction of 14 surrogate mothers using laparoscopy techniques. One recipient delivered twin kids, whereas another two recipients each.delivered a single kid The parentage of these kids was evaluated using highly polymorphic co-dominant microsatellites markers. From the present study, it was concluded that live goat kids can be produced from in vitro matured and fertilized goat embryos, to the best of our knowledge for the first time in India.

• 대한수의학회지
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• 제32권1호
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• pp.143-151
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• 1992

Sixty Four fresh and 142 frozen embryos of dairy cattle were transferred to synchronized dairy, beef or Korean Native Cattle nonsurgically at National Animal Breeding Institute from 1985 to 1990. The results obtained were as follows ; 1. The pregnancy rate of fresh embryos(39.1%) was higher than that of frozen embryos(32.4%) and average pregnancy rate was 34.5%. 2. The pregnancy rate of grade 1 embryos was higher than that of grade 2 embryos for both fresh(41.3% vs 33.3%) and frozen embryos(35.4% vs 25.6%). 3. The pregnancy rate according to development stage of fresh embryos was increased with maturity as 29.2%, 33.3%, 50.0% and 54.5% for morula, early blastocyst, blastocyst and expanded blastocyst, respectively. For frozen embryos, the pregnancy rate of blastocyst(44.4%) was higher than those of morula(31.3%) and early blastocyst(28.0%). 4. The pregnancy rate according go recipient-donor synchrony for fresh embryos was higher when the recipients exhibited estrus 1 day earlier than the donors(43.8%) than when the recipients exhibited estrus 1 day later than the donors(38.1%) or when the recipients and donors exhibited estrus at the same time(37.0%). For forzen embryos, the pregnancy rate was decreased when the recipients and donors exhibited estrus at the same time(37.9%), when the recipients exhibited estrus 1 day later than the donors(32.0%) and when the recipients exhibited estrus 1 day earlier than the donors(23.5%), in sequence. 5. The pregnancy rate of heifers was higher than that of cows for both fresh(50.5% vs 37.9%) and frozen embryos(39.7% vs 25.7%). 6. The pregnancy rate according to recipient breed for fresh embryos was higher in dairy cattle(42.1%) and beef cattle(40.%) than in Korean Native Cattle(33.3%). For frozen embryos, the pregnancy rate was decreased beef cattle(39.1%), dairy cattle(30.3%) and Korean Native Cattle(14.3%), in sequence. 7. The pregnancy rate according to equilibrium steps of glycerol and freezing rate was higher when transferred after 3-steps equilibrium and freezing by the rate of $0.3^{\circ}C$/min from $-6^{\circ}C$ to $-35^{\circ}C$ and $0.1^{\circ}C$/min to $-38^{\circ}C$(39.4%) than when transferred after 6-steps equilibrium and freezing by the rate of $0.5^{\circ}C/min$ from $-6^{\circ}C$ to $-30^{\circ}C$(30.3%).

• Asian-Australasian Journal of Animal Sciences
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• 제13권6호
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• pp.856-860
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• 2000

Cloning technology continues to capture widespread attention by the international news media and biomedical and agricultural industries. The future uses of this technology could potentially contribute to major advances in biomedical and agricultural sciences. Cloned transgenic dairy cattle possessing milk promoters directing transgenes will produce pharmaceutical proteins in their milk faster, more efficiently and less expensively than transgenic cattle created using microinjection techniques. Additionally, cloned transgenic fetuses and animals may become a source of cells, tissue and organs for xenotransplantation. Lastly, but maybe most importantly, enhanced production traits and disease resistance may be realized in animal agriculture by utilizing these new technologies. The recent advances in the cattle cloning technology are important but there are still major obstacles preventing widespread commercial use of this technology. The type of donor nucleus, recipient cytoplasm, and cloning procedures used will impact the potential number of clones produced and the uses of the technology. In addition, the new advances in cloning methodology have not improved the relatively low pregnancy rates or reduced the incidence of health problems observed in cloned offspring. These problems may require novel techniques to decipher their cause and new methods of preventing and/or diagnosing them in the preimplantation embryo. The commercial potential is enormous for cloning technology; however, little has been done to improve the efficiencies of the procedure. Improving procedural efficiencies is a critical developmental milestone especially for potential uses of cloning technology in animal agriculture.

• 한국수정란이식학회지
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• 제26권1호
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• pp.27-32
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• 2011

본 연구는 OPU 유래 한우 수정란을 이식한 대리모의 품종이 임신 기간과 산자의 생시 체중에 미치는 영향을 분석하고자 6~7일차 OPU 유래 한우 체외수정란을 한우와 젖소 대리모에 이식하여 산자를 생산하였다. 1. 대리모에 따른 평균 임신 기간(젖소: $284.4{\pm}9.8$일, 한우: $280.9{\pm}6.2$일)과 산자의 생시 체중(젖소: $27.70{\pm}7.92\;kg$, 한우: $23.56{\pm}3.75\;kg$)에서 유의적인 차이가 없었다. 2. 임신 기간은 수송아지를 생산한 대리모(한우: $283.8{\pm}6.7$일, 젖소: $287.3{\pm}8.9$일)가 암송아지(한우: $277.3{\pm}3.3$일, 젖소: $280.1{\pm}9.9$일)를 임신한 대리모보다 유의적으로 길었다(p<0.05). 단태에서도 수송아지를 분만한 대리모의 임신 기간이 유의적인 차이가 있었다(p<0.05). 3. 산자의 성별에 따른 생시 체중은 암송아지(한우 대리모: $21.5{\pm}3.8\;kg$, 젖소 대리모: $21.5{\pm}6.1\;kg$)보다 수송아지(한우 대리모: $25.2{\pm}3.1\;kg$, 젖소 대리모: $32.3{\pm}6.0\;kg$)가 무거웠으며, 수송아지의 경우 품종간 유의적 차이를 보였다. 단태에서도 수송아지가 유의 하 게 높았다(p<0.05). 이상의 결과, OPU 유래 수정란을 이식한 대리모의 임신 기간은 대리모의 품종에 따른 차이는 없었으나, 분만된 산자의 성별에 따라 유의적인 차이기 있었다. 산자의 생시 제중은 수송아지가 유의적으로 높은 생시 체중을 보였고 임신 기간도 수송아지를 분만했을 때 유의적으로 길었다. 수송아지의 경우 품종간의 차이를 보였는데, 이러한 결과는 수정란이식에 의해 생산되는 송아지의 생시 체중과 대리모의 임신 기간은 태아의 품종보다 대리모의 품종이 영향을 미치는 것으로 판단된다. 본 연구에서 젖소 대리모가 한우 대리모보다 번식 적령기의 체중이 무거우므로 대리모의 품종이 산자의 생시 체중에 영향을 미치는 것으로 판단된다.

• 대한지역사회영양학회지
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• 제8권2호
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• pp.231-239
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• 2003

The purposes of this study were to: (a) investigate the characteristics of recipients' of the non-government foodbank program, (b) examine the health and dietary related conditions of them, and (c) evaluate the benefits and effectiveness of the foodbank program from the recipients' perspective. A total of 21 groups (n = 755) and 75 individual recipients participated in the survey. The main results of the study were as follows; (a) Generally, the individual recipients were 74-year-old female, livelihood protectee, and those who received government assistance or funds from private donators as their source of livelihood. (b) The ages of group recipients varied widely, and they also received government assistance or funds from private donators as their source of livelihood. (c) Most of the donated foods were bakery and confectionery Items. rice, and milk and other dairy products. (d) Benefits such as the decrease in the recipients' food expenses and an enhancement of their nutritional statuses were identified. (Korean J Community Nutrition 8(2) : 231∼239, 2003)

• Journal of Animal Science and Technology
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• 제48권6호
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• pp.949-956
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• 2006

본 연구는 육경 조직판 이식이 사슴뿔의 성장에 미치는 영향을 구명하기 위하여 육경 조직판을 육경과 전두골 정중선 부위에 각각 이식 하였을 때 조직판의 발달, 조직판을 제공한 부위의 녹용 생산량 및 이식한 조직판의 발육성적을 조사하였으며, 그 결과를 요약하면 다음과 같다. 사슴뿔의 조직판 이식은 육경과 전두골 정중선 중앙과 좌우 양 뿔의 정수리에 이식 시 이식 성공률은 12두 중 5두로 42％이었다. 사슴뿔의 발생 정규부위가 아닌 사슴의 전두골에서 조직판 이식으로 추가적인 사슴뿔 발생을 유도할 수가 있었다. 레드디어의 경우 대조구와 조직판을 제공한 쪽의 녹용 총생산량은 각각 716, 1,071g으로 이식을 하기 위하여 절단된 쪽의 녹용 생산량이 더 많았으며, 가짓수도 조직판을 제공한 쪽이 2.3개로 대조구 1개보다 많았다. 엘크의 경우 1두에서 이마에 조직판이 이식된 뿔에서 길이 17cm, 무게 302g 녹용을 생산하였으며, 레드디어는 다음해 낙각 된 녹각에서 길이 26cm에 무게 122g을 생산하였다. 조직판 이식시 이식부위의 피부는 진피까지 제거하여야만 두개골에 이식한 조직판이 견고하게 부착되었다.이상의 결과에서 조직판 이식으로 사슴뿔 발생의 정규부위가 아닌 전두골에서 부가적인 사슴뿔을 생산할 수 있다고 사료된다.

• Current Research on Agriculture and Life Sciences
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• 제4권
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• pp.109-113
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• 1986

수정란이식에 있어서 수란우의 조건에 따른 수태율을 조사하기 위하여 생수 14 ~24개월되는 젖소 처녀우 29두와 한우 처녀우 1두, 그리고 젖소 경산우 3두 계33두의 수란우에 morulae stage부터 advanced blastocyst stage의 수정란을 각각 1개씩 이식하여 이들 수란우의 연령, 이식된 계절, 반복사용 그리고 공란우와 수란우간의 발정동기화 시차 등에 따른 수태율을 조사하였으며 그 결과를 요약하면 다음과 같다. 1. 수란우의 연령에 따른 수태율은 경산우가 100%로 좋았고 18개월 이상의 처녀우가 78%로 높았다. 14개월 이하의 어린 처녀우가 가장 나쁜 67%를 보였다. 2. 이식한 계절에 따른 수란우의 수태율을 보면 겨울(11월~1월)과 봄(2월~4월)이 각각 100%, 83%로 가을(8월~10월)의 50%보다 좋았다. 3. 1차 이식 후 불임된 수란우의 재사용에 따른 수태율은 1차에 사용된 31두 수란우 중 25두가 수태, 80.7%의 수태율을 보였으나 2차례 이용된 2두는 모두 임신되지 못했다. 4. 공란우와 수란우의 발정동기화의 시차에 따른 수태율은 -(before donor) 12 시간, -6 시간, +(after donor) 6시간, +12 시간, 그리고 +12시간 이상에서 각각 100%, 86%, 67%, 79% 그리고 50%로 공란우보다 빠른편이 수태율이 늦은 우군보다 높았고 ${\pm}12$시간 이내의 동기화된 우군에서 좋은 수태율을 보였다.

• Reproductive and Developmental Biology
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• 제38권4호
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• pp.147-158
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• 2014

Differentiated nuclei can experimentally be returned to an undifferentiated embryonic status after nuclear transfer (NT) to unfertilized metaphase II (MII) oocytes. Nuclear reprogramming is triggered immediately after somatic cell nucleus transfer (SCNT) into recipient cytoplasm and this period is regarded as a key stage for optimizing reprogramming. In a recent study (Dai et al., 2010), use of m-carboxycinnamic acid bishydroxamide (CBHA) as a histone deacetylase inhibitor during the in vitro early culture of murine cloned embryos modifies the acetylation status of somatic nuclei and increases the developmental competence of SCNT embryos. Thus, we examined the effects of CBHA treatment on the in vitro preimplantation development of porcine SCNT embryos and on the acetylated status of histone H3K9 on cloned embryos at the zygote stage. We performed the three groups SCNT: SCNT (NT), CBHA treatment at the porcine fetus fibroblast cells (PFFs) used as donor cells prior to SCNT (CBHA-C) and CBHA treatment at the porcine SCNT embryos during the in vitro early culture after oocyte activation (CBHA-Z). The PFFs were treated with a $15{\mu}M$ of CBHA (8 h) for the early culture and the porcine cloned embryos were treated with a $100{\mu}M$ concentration of CBHA during the in vitro early culture (10 h). Cleavage rates and development to the blastocyst stage were assessed. No significant difference was observed the cleavage rate among the groups (82.6%, 76.4% and 82.2%, respectively). However, the development competence to the blastocyst stage was significantly increased in CBHA-Z embryos (22.7%) as compared to SCNT and CBHA-C embryos (8.6% and 4.1%)(p<0.05). Total cell numbers and viable cell numbers at the blastocyst stage of porcine SCNT embryos were increased in CBHA-Z embryos as compared to those in CBHA-C embryos (p<0.05). Signal level of histone acetylation (H3K9ac) at the zygote stage of SCNT was increased in CBHA-Z embryos as compared to SCNT and CBHA-C embryos. The results of the present study suggested that treatment with CBHA during the in vitro early culture (10 h) had significantly increased the developmental competence and histone acetylation level at the zygote stage.

• Current Research on Agriculture and Life Sciences
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• 제6권
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• pp.145-156
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• 1988

본(本) 연구(硏究)는 생쥐에 대한 과배란반응(過排卵反應), 회수(回收)된 수정란(受精卵)의 양분율(兩分率), 양분(兩分)된 분할구(分割球)의 체외배양(體外培養) 및 이식후(移植後) 산자(産仔)로의 발달율(發達率)을 조사하기 위하여 CBA 및 C57BL 계통(系統)의 공시(供試)생쥐에서 얻어진 수정란(受精卵)을 0.5% protease로 투명대 제거 및 미세유리봉으로 양분(兩分)하여 얻어진 분할구(分割球)를 blastocyst로 배양(培養)하여 BALB/C 계통(系統)의 수란(受卵)생쥐에 이식(移植)하여 다음과 같은 결과(結果)를 얻었다. hCG 주사후(注射後) 36~42시간(時間), 48~54시간(時間), 62~66시간(時間), 72~78시간(時間)에 수정란(受精卵)을 회수(回收)한 결과(結果) 각각(各各) 2세포기(細胞期), 4세포기(細胞期), 8세포기(細胞期) 및 상실배기(桑實胚期)의 수정란(受精卵)이 대부분 회수(回收)되었으며 수당(首當) 평균(平均) 17.0개(個)의 난자(卵子)가 회수(回收)되었다. 회수후(回收後) 양분(兩分)한 8세포기(細胞期) 수정란(受精卵)의 양분율(兩分率)은 83.6%로서 상실배(桑實胚)의 65.5%에 비(比)해 유의적으로 높았으나 blastocyst로의 분할구(分割球)의 발달율(發達率)은 상실배(桑實胚)에서 얻어진 분할구(分割球)가 76.5%로서 8세포기(細胞期) 분할구(分割球)의 60.9%에 비(比)해 유의적으로 높았다. 한편 배양(培養)된 분할구(分割球)와 intact embryo에 있어서 세포기(細胞期)에 따라 각각(各各) 수란(受卵)생쥐당(當) 2개(個), 10개(個), 15개(個)씩 이식(移植)하였을 때 공(共)히 15개(個)씩 이식(移植)한 경우 수태율(受胎率)이 가장 높았으나 세포기(細胞期)에 따른 수태율(受胎率)에 있어서 유의적인 차이는 없었다. 배양(培養)된 분할구(分割球)와 intact embryo의 이식(移植)후 새깨생산율(生産率)에 있어서 8세포기(細胞期)에서는 intact embryo를, 상실배(桑實胚)에서도 intact embryo를 이식(移植)하였을때 새끼 생산율(生産率)은 각각(各各) 37.6% 및 43.4%로서 각각(各各) 배양(培養)된 분할구(分割球)를 이식(移植)하였을때 보다 새끼생산율(生産率)이 유의적으로 높았다.