• Asian Pacific Journal of Cancer Prevention
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• 제13권11호
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• pp.5405-5408
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• 2012

Curcumin (CM), a biphenyl compound, possesses anti-inflammatory, antioxidant and antimicrobial activity. MicroRNAs (miRNAs) are small noncoding RNAs which regulate gene expression and the molecular mechanisms of several biological processes. Liver fibrosis is a major cause of hepatic dysfunction and cancer and there are few effective therapies emphasizing the need for new approaches to control. The present study was conducted to investigate the effect of curcumin (CM) on liver fibrosis through modulating the expression level of miRNAs (199 and 200), the main miRNAs associated with liver fibrosis. Induction of liver fibrosis by carbon tetrachloride ($CCL_4$) was confirmed by histopathological examination. Mice were divided into 3 groups: group 1 were i.p injected with 10% $CCL_4$ twice weekly for 4 weeks and then once a week for the next 4 weeks followed by 4 weeks with olive oil only. Group 2 were i.p injected with 10% $CCL_4$ twice weekly for 4 weeks and then once a week for the next 4 weeks followed by curcumin (5 mg/mouse/day) once daily for the next 4 weeks. The third group was injected with olive oil. The expression level of miR-199 and miR-200 and some of their targeted genes were measured by real time PCR. miRNA (199 and 200) levels were significantly elevated in liver fibrotic tissues compared to control groups. Curcumin was significantly returned the expression levels of mir-199 and -200 with their associated target gene nearly to their normal levels. This is the first study that highlighted the effect of curcumin on liver fibrosis through regulation of miRNAs.

• 재활치료과학
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• 제3권1호
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• pp.67-77
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• 2014

목적 : 본 연구는 치매환자에게 과제지향적 훈련이 수단적 일상생활활동에 미치는 영향에 대해 알아보고자 하였다. 연구방법 : 본 연구는 치매환자를 대상으로 사례연구를 실시하였다. 독립변인은 과제지향적 훈련으로 공통과제와 개인과제로 주 3회 매 회기 45분씩 총 12회 실시되었다. 종속변인은 기억력(숫자외우기 검사)과 실행기능(길 만들기 검사와 시계그리기 검사), 수단적 일상생활수행능력(AMPS)의 변화였다. 결과 : 과제지향적 훈련을 통한 대상자의 실행기능이 향상되었으며, 기억력은 유의하게 변화되지 않았다. 수단적 일상생활 수행능력 결과는 임상적으로 유의하게 향상되었다. 결론 : 본 연구를 통해 과제지향적 훈련이 치매환자의 수단적 일상생활활동에 긍정적 영향을 미치는 것을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제27권12호
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• pp.2094-2103
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• 2017

Aging is associated with distinct changes in immune cells and a decline in immune function, leading to increased susceptibility to infection and reduced responses to vaccination. Certain strains of lactic acid bacteria exert beneficial effects on the immune system. Previously, we reported that Weissella cibaria JW15 isolated from kimchi possesses immune stimulatory activity in vitro. In the present study, we further investigated whether oral administration of JW15 improves immune function in aged mice. Eighteen-month-old female mice were administered JW15 daily at low (JW15-L; $1{\times}10^8CFU/mouse$) or high dosage (JW15-H; $1{\times}10^9CFU/mouse$), or with Lactobacillus rhamnosus GG (LGG) using oral gavage. Two-month-old female mice were included as healthy young mice. After 4 weeks, the mice were euthanized and immune profiles were analyzed using whole blood and spleen. In complete blood count analysis, the numbers of white and red blood cells were significantly increased in the JW15-L group compared with those in the old mouse (OM) control group. In addition, administration of either JW15 of LGG resulted in higher numbers of splenocytes in comparison with the OM group. Furthermore, proliferative potentials were higher in all probiotic groups than OM. Cytokines such as IFN-${\gamma}$ and IL-6 were secreted at higher levels in splenocytes isolated from JW15-fed mice than in OM control mice. Similarly, mRNA expression of various cytokines was altered in the JW15 groups. Collectively, these results suggest that JW15 supplementation induces immunomodulatory effects in aged mice and indicate JW15 as a potential probiotic strain to improve immune function in aged animals.

• Asian-Australasian Journal of Animal Sciences
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• 제30권6호
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• pp.755-764
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• 2017

The current review seeks to highlight the concerns that have been raised on pre-slaughter stress, contributing factors and its consequent effects on cattle behavioural responses and the quality of beef; inter-linking the activities involved from birth to slaughter. Such information is crucial in light of the consumer concerns on overall animal welfare, quality of meat and food security. Slaughter animals are exposed to different conditions during production and transportation to abattoirs on a daily basis. However; the majority of studies that have been done previously singled out different environments in the meat production chain, while conclusions have been made that the welfare of slaughter animals and the quality of meat harvested from them is dependent on the whole chain. Behaviour is a critical component used to evaluate the animals' wellbeing and it has been reported to have an effect on product quality. Apart from the influence of on-farm, transportation and abattoir conditions, the genetic background of the animal also affects how it perceives and responds to certain encounters. Stress activates the animals' hypothalamic-pituitary-adrenal activity, triggering release of various stress hormones such as catecholamines and cortisol, thus glycogen depletion prior slaughter, elevated ultimate pH and poor muscle-meat conversion. Pre-slaughter stress sometimes results to cattle attaining bruises, resulting to the affected parts of the carcass being trimmed and condemned for human consumption, downgrading of the carcass and thus profit losses.

• Asian-Australasian Journal of Animal Sciences
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• 제30권9호
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• pp.1253-1260
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• 2017

Objective: Purpose was to determine the effects of grazing system and floor type on concentrations of blood metabolites, activity of creatine kinase, body weight and carcass characteristics of castrated Nguni goats. Methods: Forty eight, 7 month old goats were randomly allocated to herding and tethering treatments from 0800 to 1300 hours and accommodated on slatted and earth floors daily. Blood samples were collected by jugular venipuncture every fifteenth day for metabolite analysis. Slaughter was done at a commercial abattoir following 5 months of monitoring. Results: Tethered goats had significantly higher concentrations of urea (5.19 mmol/L) (p<0.001), creatinine ($55.87{\mu}mol/L$) (p<0.05), total protein (64.60 g/L) (p<0.01), and globulin (49.79 g/L) (p<0.001), whereas herded goats had higher glucose (3.38 mmol/L) (p<0.001), albumin (15.33 g/L) (p<0.05), albumin/globulin ratio (0.34) (p<0.01), and body weight (24.87 kg) (p<0.001). Slatted floors caused higher (p<0.01) albumin at 15.37 g/L. The interaction of grazing system and floor type affected creatinine, total protein, globulin at (p<0.01) and albumen/globulin ratio at (p<0.01). The least creatinine concentration and albumin/globulin ratio was in herded and tethered goats that were accommodated on earth floors, respectively. The highest total protein and globulin concentrations were in serum of tethered goats that were accommodated on earth floors. The highest (p<0.05) dressing percentage (45.26%) was in herded goats accommodated on slatted floors. Conclusion: Herding of goats lowered globulin concentration, improved estimated feed intake, blood glucose and albumin concentrations, albumin globulin ratio, increased body weights and weight related carcass characteristics. Floor type had very little effects on metabolites where earth floors only reduced albumin concentration. Tethering and housing goats on earth floors resulted in double stress that increased chronic infections.

• 한국약용작물학회지
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• 제24권4호
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• pp.277-283
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• 2016

Background: The photosynthetic efficiency cool-season, semi-shade ginseng is normal at low morning temperatures, but drops at high afternoon temperatures. Therefore, optimal plant performance would be ensured if it were possible to control daily light transmission rates (LTR). Methods and Results: Plants were grown in a controlled light environment that replicated 11 AM conditions and comparatively analyzed against plant grown under normal conditions. Growth in the controlled light environment resulted in a 2.81 fold increase in photosynthetic efficiency with no change in chlorophyll content, although LTR were high due to low morning temperatures. Increased aerial plant growth was observed in the ginseng plants adapted to the controlled light environment, which in turn influenced root weight. An 81% increase in fresh root weight (33.3 g per plant on average) was observed in 4-year-old ginseng plants grown in controlled light environment compared to the plants grown following conventional practices (18.4 g per plant on average). With regard to the inorganic composition of leaves of 4-year-old ginseng plants grown in controlled light environment, an increased in Fe content was observed, while Mn and Zn content decreased, and total ginsenoside content of roots increased 2.37 fold. Conclusions: Growth of ginseng under a favorable light environment, such as the condition which exist naturally at 11 AM and are suitable for the plant's photosynthetic activity creates the possibility of large scale production, excellent-quality ginseng.

• 한국융합학회논문지
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• 제7권3호
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• pp.1-14
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• 2016

힐링요가를 수련하는 여성노인들을 대상으로 신체조성 및 신체능력, 그리고 스트레스에 미치는 영향을 알아보고 노인의 건강한 삶의 방향을 긍정적으로 제공해 줄 수 있는 자료를 제시하는데 그 목적이 있다. 연구의 참여자는 A광역시에 소재하고 있는 B복지관에 참여하는 65세 이상의 노인을 대상으로 일상생활에서 신체활동에 지장이 없는 노인으로 이 연구의 취지에 동의한 자로서 자립적으로 보행할 수 있는 16명을 선정하여 힐링요가수련 집단 8명과 통제집단 8명으로 단순무선 표본추출(simple random sampling)로 할당하여 참여하였다. 자료처리는 사후검사 측정값을 서로 비교하는 공분산 분석(Analysis of Covariance)으로 분석하였으며, 모든 통계적 유의수준은 .05로 설정하였다. 그 결과 힐링요가집단에게 12주간의 힐링요가 프로그램을 주3회, 50분씩 총 36회기를 프로그램을 실시하였으며 신체조성, 신체능력은 힐링요가집단에서 긍정적 변화를 보였으며, 스트레스 수치의 변화인 하위 요인에서 집단 간 통계적 변화를 보이지 않았지만 평균값을 비교해보면 힐링요가집단이 사회적 역할수행 및 자기신뢰에서 많은 증가를 보였고, 우울 수면장애, 일반건강 및 생명력 요인에서 통제집단보다 많은 감소를 보였다.

• 한국학교보건학회지
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• 제13권2호
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• pp.241-259
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• 2000

The purpose of this study is to inquire into the degree of health promotion activity of boys' high school students, and to find out the relationship between self-efficiency and health promotion behavior in order to offer basic data for developing an educational method or program for health promotion. The content validity of the instrument was authenticated by a professor of the science of nursing, and reliability is proved to be 'cronbach' a 'after mortifying content through a pre-test on 37 students. The collected 452 persons' data is analyzed in terms of average, percentage, t-test, ANOVA, Pearson' s Correlation Coefficient. and Multiple Stepwise Regression by 'SAS 6.12'. The analyzed data is the following; 1. The higher self-efficiency, among cognitive-perceptual factors, has a beneficial effect on health promotion behavior(r=.0687, p=.0001). 2. The acting degree of health promoting behavior appeares to be middled at 3.39 points out of 6. 3. The degree of self-efficiency appears at 6.04 points out of 10. 4. The difference in health promoting behavior according to a subject's general factors is the following health concern of parents (p=0.01), higher health concern of parents, makes health promoting behavior higher. 5. The difference of self-efficiency according to subject's general factors is affected by the health concerns of parents(p=0.0044) and between groups(p=0.0001).

• Nutritional Sciences
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• 제6권4호
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• pp.195-200
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• 2003

In recent years, the prevalence of type 2 diabetes mellitus has dramatically increased in Korea as the diet has rapidly become westernized. We determined the effect of long-term cola intakes on glucose metabolism and oxidative stress in weanling male Sprague Dawley rats consuming a moderate fat diet Thirty male rats, born from 6 female rats, were randomized into cola or water drinking groups. For 28 weeks, all rats were provided with an ad lib solid diet having 33 percent of its metabolisable energy as fat In addition, rats of the cola group were provided with ad lib cola instead of water. The daily total caloric intake did not differ between groups. The rats in the cola group consumed a higher proportion of carbohydrates, and their mean body weight and fasting serum insulin level were lower than that of the control group. Whole-body glucose disposal rates measured by an euglycemic hyperinsulinemic clamp were higher in the cola group. However, lipid peroxide levels in kidney tissue were higher in the cola group than in the control group. Superoxide dismutase activity in kidney tissues was lower in the cola group compared to the control group, while glutathion peroxidase and catalase activities were not significantly different between the two groups. In conclusion, long-term cola intakes decreased insulin resistance, but increased oxidative stress in kidney tissue due to decreased SOD activities, which may lead to kidney damage. Thus, moderate changes in insulin resistance may not affect the status of oxidative stress, and vice versa.

• Biomolecules & Therapeutics
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• 제1권2호
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• pp.244-250
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• 1993

Hematotoxicity and vascular irritation of DA-125, a new anthracycline antitumor antibiotic, were investigated in mice and rabbits. In hematotoxicity study, healthy male ICR mice were treated with DA-125 by a single intravenous injection at doses of 18 and 24 mg/kg. After 4, 8, 12 and 16 days WBC count, RBC count, hemoglobin concentration, hematocrit value, and platelet counts were measured respectively. As a positive control, 12 mg/kg of doxorubicin (DXR) was used in the same manner. Remakable reductions of WBC counts in groups treated with DA-125 or DXR were observed 4 days after administration and returned to normal range 8 days after injection in groups of DA-125 18 mg/kg and DXR 12 mg/kg. The recovery of leukopenia induced in a group of DA-125 24 mg/kg took about 16 days after administration. The RBC counts, hemoglobin concentrations and hematocrit values also decreased in all drug treated groups on day 8 and recovered thereafter. The platelet counts of groups treated with DA-125 or DXR decreased on day 4 and recovered from day 8 of experiment. Local vascular irritation of DA-125 was also assessed in rabbits. The obtained results can be summarized as follows. 1. Thrombophlebitis was not induced even after daily intravenous administration of 0.4% solution of DA-125 or 0.2% solution of DXR for 7 days. 2. Macro- and microscopic observations revealed that the irritative activity of 0.4% solution of DA-125 in blood vessels was not so much different from that of saline when they were injected once a day into vein retroauricularis of rabbits for 7 days. 3. Mild inflammatory reaction was noted around vessels in rabbits treated with 0.2% solution of DXR after consecutive intravenous infusion for more than 5 days. 4. The potencies of vascular irritation of the test solutions were summarized in the following order; saline = 0.4% DA-125<0.2% DXR. These results indicated that DA-125 showed similar pattern of hematotoxicity with DXR but was less hematotoxic than DXR, and that 0.4% solution of DA-125 did not elicit unusual toxic properties when injected through intravenous route for clinical practice.