• Journal of Advanced Marine Engineering and Technology
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• v.15 no.1
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• pp.51-58
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• 1991

Corrosion fatigue test was performed by the use of plane bending fatigue tester in marine environment having various specific resistance from 25(natural sea water) to 5000.ohm.cm. It is in order to investigate the effects of marine environmental factor on the corrosion fatigue fracture of SS41 steel. The main results obtained are as follows; 1. The aspect ratio(b/a) of corner crack growing in natural sea water is lower than that in air. 2. The surface crack growth rate(da/dN) in marine environment is faster than that in air and da/dN delaies with the specific resistance increased. 3. The experimental constant m of paris rule [da/dN=C(${\delta}$K)$^m$] decrease with the specific resistance decreased and the effect of corrosion in proportion to the specific resistance is more sensitive than that of stress intensity factor range(${\delta}$K) under region II. 4. The accelerative factor(${\alpha}$) in marine environment is about 1.1-2.7 and .alpha. is increase under the low region of stress intensity factor range(${\delta}$K). 5. The electrode potential($E_0$) gets less noble potential with the specific resistance decreased.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.21 no.10
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• pp.1626-1635
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• 1997

It is important to ensure the reliability of the first localized reactor vessel steel. To satisfy with this purpose, a study on the impact/hardness, low cycle fatigue(LCF), crack growth rate(da/dN) and fracture toughness( ) of base material(BM) and weld metal(WM) were performed under room temperature air and corrosion conditions. A summary of the results is as folows : (1) Charpy impact absorbed energy of BM was the highest value, heat affected zoon(HAZ) and the lowest, WM. The hardness of BM was similar to HAZ. (2) Coefficients of Manson equation using the monotonic tensile test data were obtained for the present material. (3) The effects of stress ratio and ambient (120.deg. C and NaCl) condition on da/dN were investigated, da/dN with NaCl condition expressed the highest value. (4) The results of Charpy V-notch impact test had good correlation with $K_{IC}$ characteristics and the lowest curve of $K_{IC}$ for BM was derived, more researches about WM and HAZ are required hereafter.

• Journal of Korean Society of Steel Construction
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• v.8 no.4 s.29
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• pp.133-133
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• 1996

Application of the relationship $da/dN=C({\Delta}K)^{m}$ is effective in the analysis of fatigue crack growth life. The values of material constant C and m have great influences on the predicted fatigue life and the relationship between fatigue crack growth rate(da/dN) and stress intensity factor range(${\Delta}K$) is effective in fatigue crack growth behavior. In this paper, fatigue crack growth behavior of the welded joints in HT60 grade TMCP(Thermo Machanical Control Process) steel have been studied. To evalute the fatigue crack growth rates of HT60 grade TMCP steel, fatigue test was performed by base metal(BM), heat affected zone(HAZ) and weld metal(WM) in TMCP steel at room temperature. We determined the relationship of $da/dN-{\Delta}K$ by correlation between C and m obtained from the Paris-Erdogan power law data supplied HT60 grade TMCP steel. The obtained results from this study indicate that fatigue crack growth rate of TMCP steel is not influenced by softening effect which occurs in the HAZ when high heat input weld is carried out. Softening effects, which affect fatigue properties. are shown that it is not affected to the fatigue growth rates significantly.

• Parasites, Hosts and Diseases
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• v.46 no.4
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• pp.279-283
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• 2008

To examine humoral immune responses in the host, we measured serum antibody levels in different strains of mice (ICR, BALB/c, and C3H) experimentally infected with Neodiplostomum seoulense. Specific IgG antibody levels were increased remarkably with little difference among 3 strains of mice infected with N. seoulense from day 7 to 35 post-infection. More target proteins of adult parasites reacted with IgG at the time when the worm recovery decreased compared with other times. More than 20 protein bands, from 14 kDa to 94 kDa in size, were separated from the crude antigen of N. seoulense adults by SDS-PAGE, and among them 26, 30, 35, 43, 54, 67, and 94 kDa proteins were the major antigenic proteins. The results suggest that significant IgG antibody responses occur against N. seoulense in mice and this may be related with expulsion of worms.

• Journal of Ocean Engineering and Technology
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• v.1 no.2
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• pp.113-122
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• 1987

Ships and offshore strrctures are exposed to the corrosive surroundings, and the extablishment of the design criteria and the elucidation on the influence by this environment are requested to maintain the safety and to demonstrate the function of the structure. In this paper, the fatigue-crack-growth behavior on the compact tension specimens of quenched, tempered HT80 grade steels and RA36 high tensile steels having a single edge fatigue cracked notch respectively, were investigated under the repeated tensile stress with constant stroke in sea water for the welded parts by shielded metal arc welding. Main results obtained are summerized as follows; 1. The fatigue-crack-growth rates da/dN in sea water appeared to be greater behavior than those in air environment at the same stress intensisy factor range $\DeltaK$. 2. The correlation data of da/dN$\DeltaK$ of the two kinds of high tensile steels in sea water showed no great difference, however, the correlation data of da/dN$\DeltaK/\sigma_y$($\sigma_y$ stands for yield strength of the material) showed that the fatigue-crack-growth behavior of RA36 plate is affected by active path corrosion(APC) mechanism, while that of HT80 grade plate is mainly affected by hydrogen embrittlement mechanism.

• The Korean Journal of Nuclear Medicine
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• v.31 no.4
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• pp.421-426
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• 1997

We evaluated the in vivo kinetics, distribution, and pharmacology of N-(4-[$^{18}F$]fluoromethylbenzyl)spiperone ([$^{18}F$]FMBS), a newly developed derivative of spiperone, as a potentially more selective radiotracer for the dopamine (DA) $D_2$ receptors. Mice received 1.9-3.7 MBq (1.8-3.6 nmol/kg) of [$^{18}F$]FMBS by tail vein injection. The time course and regional distribution of the tracer in brain were assessed. Blocking studies were carried out by intravenously preinjecting DA $D_2$ receptor blockers (spiperone, butaclamol) as well as drugs with high affinity for DA $D_1$ (SCH 23390), DA transporter (GBR 12909), and serotonin $S_2$ ($5-HT_2$) (ketanserin) sites. After injection of the tracer, the radioactivity in striatum increased steadily over time, resulting in a striatal-to-cerebellar ratio of 4.8 at 120 min postinjection. By contrast, the radioactivity in cerebellum, frontal cortex, and remaining cortex washed out rapidly. Preinjection of unlabeled FMBS (1 mg/kg) and spiperone (1 mg/kg) reduced [$^{18}F$]FMBS striatal-to-cerebellar ratio by 41% and 80%, respectively. (+)-Butaclamol (1 mg/kg) blocked 80% of the striatal [$^{18}F$]FMBS binding, while (-)-butaclamol (1 mg/kg) did not. Preinjection of SCH 23390 (1 mg/kg) and GBR 12909 (5 mg/kg) had no significant effect on [$^{18}F$]FMBS binding. Ketanserin (1 mg/kg), a ligand for the $5-HT_2$ receptors, did not cause significant inhibition either in striatum, in frontal cortex, or the remaining cortex. The results demonstrate that [$^{18}F$]FMBS labels DA $D_2$ receptors selectively in vivo in the mouse brain. It may hold promise as a selective radiotracer for studying DA $D_2$ receptors in vivo by PET.

• Food Science and Preservation
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• v.25 no.1
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• pp.1-6
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• 2018

This study investigated the immune-enhancing effects of polysaccharides extracted from Gloiopeltis furcata (red seaweed) with different molecular weights. A crude polysaccharide mixture was hydrolyzed using acid treatment (0.1 N HCl) and three molecular weight fractions were generated and filtered using centrifugation: (${\leq}10kDa$, 10 to 100 kDa, and 100 kDa. Nitric oxide (NO) production in RAW264.7 cells treated with $0.01-0.5{\mu}g/mL$ polysaccharides ${\geq}100kDa$ was $12.28-19.05{\mu}M$. Treatment with polysaccharides ${\geq}100kDa$ increased cytokine levels, including TNF-${\alpha}$ and IL-6 levels, in a dose-dependent manner. Polymerase chain reaction analysis also revealed marked increases in iNOS and COX-2 mRNA expression levels. These findings lead us to conclude that macrophage activation induced by polysaccharides ${\geq}100kDa$ was greater than that induced by polysaccharides ${\leq}10kDa$ or between 10 and 100 kDa. The polysaccharides ${\geq}100kDa$ extracted from Gloiopeltis furcata investigated herein are potentially useful as natural immune-enhancing agents. These findings provided further insights into the potential use of ${\geq}100kDa$ as immunopotentiator or new function food.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2000.11a
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• pp.91-93
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• 2000

Soybean meal(SMB) has not only been an important source of protein, but also a major source of isolfavones(IF) for poultry diet. Since the IF is one of effective phytoestrogens, evaluation of bioavailability of the phytochemicals for poultry and development of an animal model for assessment of the availability should be essential for its practical application. Present study was to develop bird model for assessing their bioavailability. No reports for such measurments with poultry could have every been found. Eight, 6-wk-old, layer-type, male birds were divided into two groups of four birds; normal (N) and colostomized(COL). Each bird was tube-fed a SMB sample at a level of 1% body weight. Samples from blood, urine and feces(COL birds) or urine+feces (N) were collected at 0, 2, 8, 15, 24 and 48 hours after the feeding. Plasma and urine samples were treated with -glucuronidase and fecal samples with 4N HCl for measurement of genistein(GE) and daidzein (DA) with HPLC set with an electrochemical detector. Maximum peaks of plasma IF appeared at 2 h after the meal for both birds groups. However the IF peak for N group was GE, while that for COL group was DA. Plasma DA levels for COL birds were always higher than those of GE, which was in opposite trend for N birds. This trend observed in the N birds is in accordance with those reported in rats fed SBM. In conclusion the results seem to suggest that colostomy as an attempt to separate urine from feces in birds affected the IF metabolism in the intestine and may not be a proper bird model to measure the IF bioavailability.

• Korean Journal of Microbiology
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• v.38 no.2
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• pp.67-73
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• 2002

The cellular responses of soil-borne bacterium, Pseudomonas sp. HK-6 to explosive 2,4,6-trinitrotoluene (TNT) were examined. Two stress shock proteins (SSPs), approximately 70-kDa DnaK and a 60-kDa GroEL were found in HK-6 cells in response to TNT. Analyses of SDS-PAGE and Western blot using anti-DnaK and GroEL revealed that SSPs were induced in HK-6 cells exposed to 0.5 M of TNT far 6-12 hrs. The maximum induction of proteins was achieved at 8-hr incubation point after HK-6 cells'exposure to TNT. Similar SSPs were found to be induced in HK-6 cells by heat shock (shift of temperature, from $30^{\circ}C$ to $42^{\circ}C$) or cold shock (shift of temperature,$30^{\circ}C$ to $4^{\circ}C$).2D-PAGE of soluble protein tractions from the culture of Pseudomonas sp. HX-6 exposed to TNT demonstrated that approximately 450 spots were observed on the silver stained gels ranging from pH 3 to pH 10. Among them, 12 spots significantly induced and expressed in response to TNT were selected and analyzed. Approximately 60-kDa protein, which was assumed highly expressed on the gel, was used for amino acid sequencing. N-terminal microsequencing with in-gel digestion showed that N-terminal sequence of the TNT-induced protein, <$^1XXAKDVKFGDSARKKML^17$, shared extensive similarity with $^1XXAKDVKFGDSARKKML^17$, N-terminal sequence of (P48216) GroEL of Pseudomonas putida.

• Korean Journal of Radiology
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• v.23 no.10
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• pp.976-985
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• 2022

Objective: To compare the clinical and radiological features of various etiologies of chronic diffuse lacrimal gland enlargement. Materials and Methods: We retrospectively reviewed 91 consecutive patients who underwent surgical biopsy for chronic diffuse lacrimal gland enlargement and were diagnosed with non-specific dacryoadenitis (DA) (n = 42), immunoglobulin G4-related dacryoadenitis (IgG4-RD) (n = 33), and lymphoma (n = 16). Data on patient demographics, clinical presentation, and CT imaging findings (n = 73) and MRI (n = 43) were collected. The following radiologic features of lacrimal gland enlargement were evaluated: size, unilaterality, wedge sign, angle with the orbital wall, heterogeneity, signal intensity, degree of enhancement, patterns of dynamic contrast-enhanced, and apparent diffusion coefficient value. Radiological features outside the lacrimal glands, such as extra-lacrimal orbital involvement and extra-orbital head and neck involvement, were also evaluated. The clinical and radiological findings were compared among the three diseases. Results: Compared to the DA and IgG4-RD groups, the lymphoma group was significantly older (mean 59.9 vs. 46.0 and 49.4 years, respectively; p = 0.001) and had a higher frequency of unilateral involvement (62.5% vs. 31.0% and 15.2%, respectively; p = 0.004). Compared to the IgG4-RD and lymphoma groups, the DA group had significantly smaller lacrimal glands (2.3 vs. 2.8 and 3.3 cm, respectively; p < 0.001) and a lower proportion of cases with a wedge sign (54.8% vs. 84.8% and 87.5%, respectively; p = 0.005). The IgG4-RD group showed more frequent involvement of the extra-orbital head and neck structures, including the infraorbital nerve (36.4%), paranasal sinus (72.7%), and salivary gland (58.6%) compared to the DA and lymphoma groups (4.8%-28.6%) (all p < 0.005). Conclusion: Patient age, unilaterality, lacrimal gland size, wedge sign, and extra-orbital head and neck involvement differed significantly different between lymphoma, DA, and IgG4-RD. Our results will be useful for the differential diagnosis and proper management of chronic lacrimal gland enlargement.