• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.35-35
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• 2005

Recombinant human $interferon-{\beta}-1a(rIFN-{\beta})$ is a single glycosylated protein (at N80, 1N) with anti-viral activity. However, present drugs have a relatively short serum half-life of $rIFN-{\beta}$, thus patients suffer from frequent $infections.^{1)}$ To improve its half-life, eight glycosylation analogs were prepared, which have additional N-linked glycosylation consensus sequences (N-X-T/S) within the $IFN-{\beta}$ molecule and/or at C-terminal. Each $rIFN-{\beta}$ analog was examined for the presence of additional N-linked glycosylation and the maintenance of anti-viral activity in CHO cells. The molecular weights of five analogs were not changed. However, two analogs, R27T within $rIFN-{\beta}$ (27 kDa, 2N) and GNITVNITV at C-terminal (29kDa, 2N), showed a clear increase in molecular weights, compared to native $rIFN-{\beta}$ (23 kDa, 1N). And another combined analog of R27T+GNITVNITV showed increased molecular weight (33 kDa, 3N). It was confimed that the molecular weight increment of analogs was caued by the N-linked glycosylation with the treatment of N-glycansae. In the case of anti-viral activity, the analog GNITVNITV showed a reduction in activity compared to native $IFN-{\beta}$, whereas the analogs R27T and R27T+GNITVNITV were found to have distinctly increased activities. Pharmacokinetic study in rats also disclosed that the analogs R27T and R27T+GNITVNITV had 2 3 fold increased serum half-life, respectively. In conclusion, the addition of N-linked glycosylation in $rIFN-{\beta}$ increased serum half-life, thereby its less frequent administration will be expected.

• Journal of dental hygiene science
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• v.8 no.3
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• pp.109-115
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• 2008

Two t-amines, N,N-dimethylaniline (MA), N,N-dimethyl-p-toluidine (MPT), were investigated as new visible light amine initiators for a dental resin composite of UDMA in order to improve photopolymerization effect. Three t-amines mixed with three photosensitizers, camphorquinone(CQ), 1-phenyl-1,2-propane dione(PD) and diacetyl (DA), respectively. And then this mixtures are added to resin monomer, UDMA. Photopolymerization efficiency of UDMA was studied through the use of FT-IR absorption spectroscopy. The photopolymerization effect of amine initiators were compared with that of 4-(dimethylamino)ethyl methacrylate (AEM), the most widely used photoinitiator. The photopolymerization efficiency of UDMA containing the amine initiator increased with irradiation time. The relative polymerization efficiency containing the CQ photosensitizer increase was in the order: AEM < MPT < MA. And the relative polymerization efficiency containing the PD photosensitizer increase was in the order: MPT < AEM < MA. This result shows that MA is most efficient amine initiator with CQ and PD.

• International Journal of Industrial Entomology and Biomaterials
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• v.2 no.2
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• pp.167-172
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• 2001

The vitellin of firefly, Pyrocoelia rufa, is composed of three polypeptides, designated Vn1 (175 kDa), Vn2 (160 kDa) and Vn3 (45 kDa) in SDS-polyacrylamide gel electrophoresis. Three subunits of vitellin were presented in the female adult hemolymph, ovary and egg extracts, but not observed in the male. This vitellin was purified from the eggs of P. rufa by the FPLC techniques, anion exchange chromatography and gel permeation chromatography. In nature, vitellin of P. rufa has molecular weight of 400 kDa. Western blot analysis using polyclonal antiserum against purified vitellin showed that the antiserum was reacted with the three polypeptides, Vnl, Vn2 and Vn3 from the female adult hemolymph, ovary and egg extracts. Amino acid residues at N-terminus of three subunits were sequenced. The N-terminal sequences of large subunits, Vnl and Vn2, were similar to each other, But, the N-terminal sequences of small subunits Vn3, did not have any signnificant homology with large subunits.

• Magazine of the Korean Society of Agricultural Engineers
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• v.44 no.7
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• pp.64-73
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• 2002

This study was performed to evaluate effects of physical characteristics of both watershed and reservoir on nutrient-chlorophyll relationship in Korean reservoirs. Simple linear models were developed with published data in Korea including 415 reservoirs and 11 multi-purpose dams, and physico-chemical parameters of reservoirs and characteristics relationship of models were analyzed. Theoretical residence time in Korean reservoirs was strongly correlated with the ratio of TA/ST (drainage area + surface area / storage volume) in the logarithmic function. As a result of monthly nutrients-chlorophyll-a regression analysis, significant Chl-a-TP relationship appeared during May～July. The high Chl-a yields per total phosphorus appeared during this time (R$\^$2/=0.51, p<0.001, N= 1088). Chlorophyll-a demonstrated much stronger relationship with TP. than TN. Seasonal algal-nutrient coupling were closely related with N:P ratio in the reservoir water, and it was, in turn, dependent on the monsoon climatic condition (precipitation). Based on the results of regression analysis and high N:P ratio, a major limiting factor of algal growth appeared to be phosphorus during this time. Unlikely TA/ST ratio, DA/SA ratio (drainage area f surface area) was likely to influence directly on the nutrient-Chl-a relationship, indicating that if storage volume and inflowing water volume were the same, algal biomass could be developed more in reservoirs with large surface area. Thus, DA/SA ratio seemed to be an important factor to affect the development of algal biomass in Korean reservoirs. With low determination coefficient of TP-Chl-a relationship, our findings indicated not only nutrient (phosphorus) but also other physical factors, such as DA/SA ratio, may affect algal biomass development in Korean reservoirs, where actual residence time appears to be more closely related to reservoir surface area rather than storage volume.

• Biomolecules & Therapeutics
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• v.5 no.2
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• pp.202-210
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• 1997

Protective effect of DA-9601, an extract of Artemisia Herb, against ethanol-induced gastric mucosal injury was evaluated in rats. In the prophylactic study, DA-9601 exhibited total protection (99.4%) against absolute ethanol-induced gastropathy, And the protective effect of DA-9601 lasted up to 2 hours, which was longer than those of other contemporary mucoprotectants. In the treatment study, DA-9601 significantly facilitated the healing of 70% ethanol-induced mucosal damage, which was superior to cetraxate, a commonly used anti-ulcer drug. The mechanisms of mucoprotection of DA-9601 were also assessed. DA-9601 increased the release of prostaglandin E$_2$ from murine neutrophils in a dose-dependent manner in vitro. The cytoprotective effect of DA-9601 against ethanol-induced mucosal damage was significantly diminished by the concommitant injection of N$\omega$-nitro-L-arginine methyl ester (L-NAME, 5 mg/kg, i.v.), a non-specific nitric oxide (NO) synthase inhibitor, while it was not affected by preinjection of indomethacin (5 mg/kg, s.c.), a prostaglandins-depletor. And it was found that DA-9601 significantly enhanced adaptive cytoprotective action of 10% ethanol against absolute ethanol (56.9$\pm$6.5 vs 23.0$\pm$3.3 mm$^2$, p<0.05, mean$\pm$SEM), though its exact underlying mechanism remains to be clarified. The present fin[lings demonstrate that DA-9601 exerts gastroprotecticv actions for the stomach against ethanol through several different underlying mechanisms, in which prostanglandins and NO are involved. In conclusion, the results obtained suggest that DA-9601 can be useful both in prevention and treatment of ethanol-induced gastric damage.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.11
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• pp.1575-1581
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• 2005

This study was conducted to determine the characteristics of IGFBPs in plasma of steers, and to profile the relationship between growth and plasma IGF-1 and IGFBPs with aging in Holstein steers. Four blots of IGFBP at molecular weights of 38-43, 34, 29-32 and 24 kDa bands were detected by western ligand blot assay using $^{125}I-IGF-1$. On the basis of immunoblotting with anti-bovine IGFBP-2 and -3 antiserums, we observed the band for IGFBP-2 at approximately 34 kDa, and the IGFBP-3 band was detected at 38-43 kDa and 34 kDa in adult steers and calves. The IGFBP-3 antiserum used on the blots exhibited significant cross-reactivity with 34 kDa IGFBP-2. Furthermore, the 38-43 kDa IGFBP-3 bands were reduced to a 36 kDa band after deglycosylation, whereas the 34 kDa IGFBP-2 was intact. The plasma IGF-1, IGFBP-3 and other IGFBPs showed stability throughout a whole day. The change in live weight was found to be positively correlated to the plasma IGF-1 concentration (r = 0.6801, n = 64, p<0.05) and plasma IGFBP-3 (r = 0.6321, n = 64, p<0.05), while inversely correlated to plasma IGFBP-2 (r = -0.2919, n = 64, p<0.05). Furthermore, plasma IGF-1 was positively correlated to plasma IGFBP-3 (r = 0.6191, p<0.001), but was not correlated to plasma IGFBP-2. The portion of IGFBP-2 for total IGFBPs in calves was higher than in adult steers (p<0.05) and was decreased with growth, whereas that of IGFBP-3 was increased with increased live weight (p<0.05). The ratio IGFBP-3 for IGFBP-2 (BP-3/BP-2) was increased with growing of liveweight. Therefore, the changes in plasma IGF-1 level with increased liveweight may be related to the changes in plasma IGFBP-3 level and IGFBP-2 may give an important role in anabolic action of IGF-1 with the growth of body during calfhood in Holstein steers.

• BMB Reports
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• v.31 no.3
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• pp.264-268
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• 1998

Dopamine (DA) acts on swimming motor neurons (SMNs) of Polyorchis penicillatus as an inhibitory neurotransmitter by hyperpolarizing their membrane potentials, which results from the activation of voltagesensitive potassium channels mediated through a $D_2-type$ receptor. In addition, DA, and not the hyperpolarized membrane potential, directly decreased the input resistance of SMNs by ca. 50% from 1.42 to 0.68 $G{\Omega}$. It strongly indicates that DA can shunt other excitatory synaptic signals onto SMNs where DA usually elicited much greater responses in their neurites than soma. All these evidences suggest that DA may operate in this primitive nervous system in dual modes as an inhibitory neurotransmitter and neuromodulator as well.

• BMB Reports
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• v.29 no.2
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• pp.151-155
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• 1996

To swimming motor neurons (SMNs) of Polyorchis penicillatus, a hydrozoan medusae, dopamine (DA) acts as an inhibitory neurotransmitter by hyperpolarizing its membrane potential and decreasing its firing rate as well. Such an inhibitory action of DA is caused by an increased permeability to potassium (K) ions. To investigate whether voltage-gated K channels are directly responsible for the membrane hyperpolarization induced by DA, we employed whole-cell voltage clamp configuration. One ${\mu}M$ DA applied to SMNs increased the peak and rear values of voltage-gated K currents by 37 and 54%, respectively, in a reversible manner. Combined with subtraction analysis, this result suggests that the outflux of K ions by DA in SMNs occurs mainly through rectifier-like K channels.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.586-589
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• 2003

Previously we reported the cloning and sequence analysis of a CFTase gene from Bacillus polymyxa. CFTase was divided into five distinct regions. In order to understand a role of the N-terminal repeat region on the function of CFTase from Bacillus polymyxa MGL21, deletion mutantCFTase ${\Delta}N$ was prepered. Recombinant protein was overproduced in E. coli as inclusion body, solubilized in bufer containing 8M urea, and refolded in the phosphate buffer. The molecular weight of the purified wild type CFTase and CFTase ${\Delta}N$ were 148kDa , 108kDa, respectively.

• Transactions of the Korean Society of Automotive Engineers
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• v.7 no.6
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• pp.258-269
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• 1999

It is important to evaluate fatigue damage of in-service material in respect to assure safety and remaining fatigue life in structure and mechanical components under cyclic load . Fatigue damage is represented by mathematical modelling with crack growth rate da/dN and cycle ration N/Nf and is detected by X-ray diffraction and ultrasonic wave method etc. But this is estimated generally by single parameter but influenced by many test conditions The characteristics of it indicates fatigue damage has complex fracture mechanism. Therefore, in this study we propose that back-propagation neural networks on the basis of ration of X-ray half-value breath B/Bo, fractal dimension Df and fracture mechanical parameters can construct artificial intelligent networks estimating crack growth rate da/dN and cycle ratio N/Nf without regard to stress amplitude Δ $\sigma$.