• Communications of the Korean Institute of Information Scientists and Engineers
• /
• v.29 no.2
• /
• pp.101-109
• /
• 2011

• Journal of Korean Medical Ki-Gong Academy
• /
• v.2 no.1
• /
• pp.89-99
• /
• 1998

• Microbiology and Biotechnology Letters
• /
• v.37 no.4
• /
• pp.350-354
• /
• 2009

A Gram-negative, alginate lyase-producing bacterium was isolated from the Haeundae Coast, Korea. The isolated strain N7151-6 produced alginate lyase. The optimal temperature and pH for growth were found to be $30^{\circ}C$ and pH 8.0, respectively. This strain can be grown at the NaCl concentration of 0-7% (w/v). Analysis of 16S rDNA sequence and physiological profiling indicated that the strain N7151-6 belonged to Pseudomonas sp. The enzyme alginate lyase produced by Pseudomonas sp. N7151-6 was partially purified by ultrafiltration (MWCO= 30 kDa). The optimum pH and temperature for the activity of the purified enzyme were found to be 7.0 and $30^{\circ}C$, respectively. The enzyme was stable at the pH range of 5.0-9.0 and temperature range of $23-30^{\circ}C$. The total activity of alginate lyase produced was reached about 110 unit/L.

• The Korean Journal of Mycology
• /
• v.31 no.3
• /
• pp.168-174
• /
• 2003

In this study, Cordyceps militaris was grown in a liquid medium containing colloidal chitin. A chitinase was purified from the supernatant or cultured medium by ammonium sulfate fractionation, DEAE-Sephadex A-25 and Sephadex G-50 column chromatography. Optimum temperature and pH of this enzyme were $35^{\circ}C$ and 5.5, respectively. The molecular weight of the chitinase was estimated to be 48.5 kDa by SDS-PAGE and its Km value was 0.57 mM. The activity of this enzyme was inhibited by $Cu^{2+},\;Mn^{2+},\;Hg^{2+},\;Zn^{2+},\;CO_{3}^{2-},\;SO_4^{2-},\;CN^-,\;ion,\;and\;OCN^-$ maleic anhydride, acetic anhydride or N-bromo succinimide, especially strongly inhibited by sodium cyanate for 84.0 percentage. But its activity wag slightly stimulated by $Mg^{2+}\;and\;K^+$ ion, respectively. The products formed during hydrolysis of the hexa-N-acetylchitohexaose with this enzyme were N,N'-diacetylchitobiose and N,N',N'-triacetylchitotriose. These results imply that this purified enzyme may be an endo-chitinase.

• Parasites, Hosts and Diseases
• /
• v.56 no.1
• /
• pp.11-19
• /
• 2018

In Brazil, visceral leishmaniasis (VL) is expanding and becoming urbanized, especially in non-endemic areas such as the State of Rio Grande do Sul. Considering that infected dogs are the main reservoir for zoonotic VL, this study evaluated the prevalence of canine visceral leishmaniasis (CVL) in the metropolitan area of Porto Alegre, a new area of expansion of VL in Brazil. Serum and plasma from 405 asymptomatic dogs from the municipalities of Canoas (n=107), $S\tilde{a}o$ Leopoldo (n=216), and Novo Hamburgo (n=82) were tested for CVL using immunochromatographic ($DPP^{(R)}$) and ELISA $EIE^{(R)}$ assays (2 assays officially adopted by the Brazilian government for the diagnosis of CVL) and real-time PCR to confirm the results. There was no agreement among serological and real-time PCR results, indicating that the Leishmania infection in asymptomatic animals with low parasite load, confirmed by negative parasitological tests (smears and parasite culture), need to be evaluated by molecular methods. The prevalence of LVC in the metropolitan region of Porto Alegre, confirmed by real-time PCR was 4% (5.6% in Canoas and 4.6% in $S\tilde{a}o$ Leopoldo). The use of molecular method is essential for accurate diagnosis of CVL, especially in asymptomatic dogs in non-endemic areas.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1993.04a
• /
• pp.157-157
• /
• 1993

TMI는 알라닌과 글리신이 풍부한 18개의 아미노산으로 이루어져 분자량이 약 1,400Da인 물질이었으며, 가수분해 효소 처리후 억제력의 변화를 보이지 않았고, Porcine liver, spleen, testis에서 정제한 PM II에 대하여 TMI가 비상경적 억제작용을 하는 것으로 나타났으며, Ki value는 각각 300 nM, 250 nM 297 nM이었다. 그리고 돼지 간장에서 부분정제한 PM I, PM II, PM III, phospholipid methytransferase에 대한 정제된 TMI의 영향을 조사해 보면 각각 33%, 47%, 31%, 35%의 억제효과를 나타내었다.

• Bulletin of the Korean Chemical Society
• /
• v.35 no.2
• /
• pp.347-348
• /
• 2014

• Journal of Ocean Engineering and Technology
• /
• v.13 no.3 s.33
• /
• pp.108-113
• /
• 1999

In this study, CT specimens were prepared from ASTM A516 Gr. 65 which was used for pressure vessel plates for moderate and lower temperature service. Fatigue crack growth test was carried out in the environment of low temperature of $10^{circ}C, -10^{circ}C, -30${circ}C\;and\;-50^{circ}C$ and in the range of stress ratio of R=0.05 and 0.3 by means of opening mode displacement. Based on these test results, the characteristics from temperature and stress ration were shown as follows. 1) As the stress ratio, R increased da/dN and ${Delta}K$ of 2nd stage gradually decreased. And as R decreased, the effect of temperature became greater and greater. 2) As the temperature descended, da/dN decreased on a certain ${Delta}K$, and ${Delta}K$ did in a same da/dN. And the stress ratio, R exerted greater influence at the lower temperature. 3) The fatigue crack growth constant, m increased at $10^{circ}C$ and $-10^{circ}C$, snd decreased at $-30^{circ}C$ and $-50^{circ}C$ following the increment of stress ratio R. And m increased along with the reduction of temperature greatly decreased at $-30^{circ}C$ to come close to two(2).

• Journal of Power System Engineering
• /
• v.3 no.2
• /
• pp.51-56
• /
• 1999

Fatigue crack propagation rates and characteristics of the SA516-70 steel which is used for the low temperature pressure vessels, were studied in the room temperature of $10^{\circ}C$ and low temperature ranges of $-10^{\circ}C,\;-30^{\circ}C,\;-50^{\circ}C,\;and\;-70^{\circ}C$ with stress ratio of R=0.05. The obtained experimental results are as follows; 1) In the logarithmic relationship between the fatigue crack propagation rate(da/dN) and stress intensity factor K, the linear relationship was obtained up to da/dN > $8{\times}10^3$ mm/cycle in the same of room temperature, but in low temperature case, the relationship was extended to the range of low crack propagation rate. 2) The lower limit stress intensity factor of SA516-70 ${\Delta}K_{th}\;was\;23MPa\sqrt{m}$ and in the case of low temperature $-50^{\circ}C\;and\;-70^{\circ}C$, the crack propagation rate da/dN which showed a linear relation, reached rapidly to the ${\Delta}K_{th}$. As the results, the crack propagation rates of $-50^{\circ}C\;and\;-70^{\circ}C$ were lower than that of room temperature and according to the testing temperature the rates were decreased rapidly to the ${\Delta}K_{th}$. 3) On the relationship between the stress intensity factor ${\Delta}K$ and the track propagation cycle, the stress intensity factors of low cycle region was rapidly increased at low temperature, but ${\Delta}K$ was increased rapidly at room temperature of high cycle. 4) On the relationship between the fatigue crack propagation rate and cycle, the fatigue crack propagation rate showed higher gradient in the room temperature than the low temperature due to the increment in ductility at low temperature.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
• /
• 2000.10a
• /
• pp.240-245
• /
• 2000

Fatigue crack propagation rates and characteristics of the SA516-60 steel which is used for the low temperature pressure vessels, were studied in the room temperature of $25^{\circ}C$ and low temperature ranges of $10^{\circ}C,\; -10^{\circ}C,\; -30^{\circ}C,\; -50^{\circ}C, \;and\; -70^{\circ}C4 with stress ratio of R=0.05. The obtained experimental results are as follows; 1) In the logarithmic relationship between the fatigue crack propagation rate(da/dN) and stress intensity factor K, the linear relationship was obtained up to da/dN 〉$8\times10^{-3}$/mm/cycle in the same of room temperature, but in low temperature case, the relationship was extended to the range of crack propagation rate. 2) The lower limit stress intensity factor of SA516-60 $\DeltaK_{th}$ was 15.8MPa and in the case of low temperature $-50^{\circ}C\; and\; -70^{\circ}C$, the crack propagation rate da/dN which showed a linear relation, reached rapidly to the $\DeltaK_{th}$/. As the results, the crack propagation rates of $-50^{\circ}C\; and\; -70^{\circ}C$ were lower than that of room temperature and according to the testing temperature the rates were decreased rapidly to the $\DeltaK_{th}$/. 3) On the relationship between the stress intensity factor $\DeltaK$ and the crack propagation cycle, the stress intensity factors of low cycle region was rapidly increased at low temperature, but $\DeltaK$ was increased rapidly at room temperature of high cycle. 4) On the relationship between the fatigue crack propagation rate and cycle, the fatigue crack propagation rate showed higher gradient in the room temperature than the low temperature due to the increment in ductility at low temperature.