• Food Science of Animal Resources
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• v.21 no.4
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• pp.367-373
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• 2001

Glycoproteins PAS-6(50 kDa) and -7(47 kDa) from the bovine milk fat globule membrane share a common protein core but differ in their carbohydrate moiety. We have analyzed and proposed the structures of the N-linked sugar chains of PAS-7 by Oxford Glyco System(OGS) RAAM2000. The N-linked sugar chains were liberated from PAS-7 by hydrazinolysis and, after modifying the reducing ends with 2-aminobenzamide(2-AB), were separated into one neutral(7N, 55%) and two acidic(7M, mono-, 43%; 7D, di-, 2%) sugar chain groups. 7N was finally separated into 5 chains(a, b, c, d, and e), respectively. The structure of this 2AB-neutral sugar chain was determined by sugar analysis, exoglycosidase digestion with OGS glycosidase Kit and OGS RAAM2000 system. The results show that fraction e was the same of reported 7N1A, the biantennary complex type with a fucose on reducing end and two N-acetyllactosamine branch on non-reducing end. Therefore, it was proved that OGS RAAM2000 method is in conformity with conventional analysis of sugar chain structure from bovine PAS-7.

• Korean Journal of Veterinary Research
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• v.44 no.4
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• pp.625-635
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• 2004

Trichnuris suis does not excrete eggs during larval stage as well as in particular adult stage, It is impossible to diagnose by use of fecal examination method in those periods. Therefore, serological diagnostic method can be very useful for those stages. In order to produce monoclonal antibody, specific somatic and secretory-excretory (SE) antigens of T. suis were identified and analyzed by SDS-PAGE and Western blot. Monoclonal antibody-producing hybridoma cells were cloned, which were made of popliteal lymph node of BALB/c mice immunized with a 45 kDa somatic antigen of T. suis. Five clones (1B9, 2C4, n2C5, 2D7 and 2D8) showing strong responses to T. suis antigens were selected and the isotype identified. All monoclonal antibodies were IgG1 isotype and the light chains were k chain. Established monoclonal antibodies reacted specifically to somatic and SE antigens of T. suis and did not cross-reacted to antigens of ascaris suum, trichuris vulpis, or Trichinella spiralis. The sensitivity of somatic and SE antigens against these monoclonal antibodies were significant (p<0.01) associated with those of positive and negative sera.

• Journal of the Korean Institute of Gas
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• v.25 no.6
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• pp.53-65
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• 2021

While the hydrogen refueling station is rapidly expanded and installed, the safety inspection of the hydrogen pressure vessel in the station should be very important. Of these, according to ASME, hydrogen embrittlement tests must be performed for hydrogen vessel that store hydrogen above a certain pressure. The main test method for hydrogen embrittlement inspection is to carry out fracture tests and fatigue fracture tests in a high pressure hydrogen atmosphere, which allows the durability limit of the pressure vessel to be measured and the endurable limit to be determined in the hydrogen atmosphere. In detail, the critical crack depth can be calculated by the stress intensity factor(K), and the service life can be determined by da/dN (fatigue growth rate). API579-1/ ASME FFS-1 part 9 exemplifies the calculation method according to the mode of crack-like flaws, but for various shapes such as plates and cylinders, there are about 55 modes according to the shape and location of the crack. Due to the fairly complex formula, it is not easily accessible. In this study, we will show you how to calculate fracture mechanics numerically via Excel and VBA. In addition, this was applied to analyze the effects of the thickness and inner diameter of the pressure vessel on the service life.

• Transactions of the Korean Society of Mechanical Engineers
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• v.12 no.3
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• pp.473-480
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• 1988

In this study, a method that can realize the effect of residual stresses on fatigue crack propagation has been proposed. This method requires that the relaxation quantities of residual stresses are measured by experimental method according to fatigue cycles and are employed to the experimental results of base materials by introducing the concept of superposition. SS41 material has been analyzed as an example. The results show that the relaxation factor of residual stresses for fatigue cycles is .alpha.$_{i}$= 1/0.6903+0.0380 $a_{I}$ the effective stress ratio for fatigue crack propagation length is ( $R_{eff}$)$_{i}$ = 1/1.1274+0.0118$_{I}$ and the fatigue crack propagation rate for relaxation quantities of residual stresses is (da/dN)$_{t}$ = 0.5 $K_{cf}$ - .DELTA.K/{1-1/1.1274+0.0118$_{ai}$ } $K_{cf}$ - .DELTA. K(da/dN), for stress ratio /R=0.5.io /R=0.5.5.

• Transactions of the Korean Society of Automotive Engineers
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• v.11 no.5
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• pp.219-226
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• 2003

The fatigue crack growth behavior of the SA516/60 steel used for pressure vessels was examined experimentally at room temperatures $25^{\circ}C$,$-30^{\circ}C$, $-60^{\circ}C$, $-80^{\circ}C$, $-100^{\circ}C$ and $-120^{\circ}C$ with stress ratio of R=0.05, 0.1 and 0.3. fatigue crack propagation rate da/dN related with stress intensity factor range $\Delta$K was influenced by stress ratio in stable than fatigue crack growth (Region II) with an increase in $\Delta$K. The resistance of fatigue crack growth at low temperature is higher compared with that at room temperature, which is attributed to the extent of plasticity-induced by compressive residual stress according to the cyclic loads. Fractographic examinations reveal that the differences of the fatigue crack growth characteristics between room and low temperatures are explained mainly by the crack closure and the strengthening due to the plasticity near the crack tip and roughness of the crack faces induced.

• Journal of Microbiology and Biotechnology
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• v.19 no.4
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• pp.358-361
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• 2009

The purified endochitosanase(Mw 41 kDa) from bacterium Bacillus cereus D-11 hydrolyzed chitooligomers $(GlcN)_{5-7}$ into chitobiose, chitotriose, and chitotetraose as the final products. The minimal size of the oligosaccharides for enzymatic hydrolysis was a pentamer. To further investigate the cleavage pattern of this enzyme, chitooligosaccharide alcohols were prepared as substrates and the end products of hydrolysis were analyzed by TLC and HPLC. The chitosanase split $(GlcN)_4GlcNOH$ into $(GlcN)_3+(GlcN)_1GlcNOH$, and $(GlcN)_5GIcNOH$ into $(GlcN)_4+(GlcN)_1GlcNOH$ and $(GlcN)_3+(GlcN)_2GlcNOH$. The heptamer $(GlcN)_6GlcNOH$ was split into $(GlcN)_5$ [thereafter hydrolyzed again into $(GlcN_3+(GlcN)2]+(GlcN)_1GlcNOH$, $(GlcN)_4+(GlcN)_2GlcNOH$, and $(GlcN)_3+(GlcN)_3GlcNOH$, whereas $(GlcN)_{1-3}GlcNOH$ was not hydrolyzed. The monomers GlcN and GIcNOH were never detected from the enzyme reaction. These results suggest that D-11 chitosanase recognizes three glucosamine residues in the minus position and simultaneously two residues in the plus position from the cleavage point.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.4 no.1
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• pp.125-136
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• 1984

In the weled structures, fatigue fracture mainly depends upon crack growth behavior. Specially anisotropy of crack growth orientation and welding direction become important factor of fracture in the welding jone. When fatigue stressed steel welded with nonfatigue stressed steel, at the low stress intensity factor range, residual stress become more important factor of growth behavior then properties of base metal but when the crack growth in the weld metal, toughness of weld metal become the most important factor. Especially nonhomgeniety of toughness for the weld metal make more scatter the relations of $da/dN-{\Delta}K$.

• Journal of Ocean Engineering and Technology
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• v.6 no.2
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• pp.76-84
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• 1992

In this study, corrosion fatigue test of SAPH45 steel was performed by the use of plane behavior of base metal (BM) and heat affected zone (HAZ) of SAPH45. The main results obtained are as follows: 1) The more aspect ratio (b/t) of corner crack decreases, the more aspect ratio (b/a) takes greatly effect by corrosion. 2) The correlation between the stress intensity factor range ($\Delta$k) and crack growth rate (da/dN) for weldment in seawater is given by Paris rule as follow: da/dN=C($\Delta$K) super(m). Where m is constant, and the value is 3.82-3.84. 3) The accelerative factor ($\alpha$) of BM and HAZ under seawater is about 1.1-1.9, and ($\alpha$) of HAZ increases more and more under the low $\Delta$K region. 4) HAZ is more susceptible to corrowion than BM because of potential of electrode (E sub(c)) of HAZ becomes more less noble potential than that of BM.

• Journal of Ocean Engineering and Technology
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• v.14 no.3
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• pp.78-83
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• 2000

In this study, CT specimens were prepared from AST SA516 Gr. 70 which was used for pressure vessel plates for room and low temperature service. And we got the following characteristics from fatigue crack growth test carried out in the environment of room and low temperature at 25$^{\circ}C $, -60$^{\circ}C $, -80$^{\circ}C $ and -100$^{\circ}C $ and in the range of stress ratio of 0.05, 0.3 by means of opening mode displacement. At the constant stress ratio, the threshold stress intensity factor range ${\delta} K_{th}$ in the early stage of fatigue crack growth (Region I) and stress intensity factor range $\delta $K in the stable of fatigue crack growth (Region II) were increased in proportion to descending temperature. It was assumed that the fatigue resistance characteristics and fracture strength at low temperature is considerable higher than that of room temperature in the early stage and stable of fatigue crack growth region. The straight line slope relation of logarithm da/dN -$\delta $K in Region II, that is, the fatigue crack growth exponent m increased with descending temperature at the constant stress ratio. It was assumed that the fatigue crack growth rate da/dN is rapid in proportion to descending temperature in Region II and the cryogenic-brittleness greatly affect a material with decreasing temperature.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2000.10a
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• pp.147-153
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• 2000

The fatigue crack propagation behavior of the SA516/70 steel which is used for pressure vessels was examined experimentally at room temperature, $150^{\circ}C$, $250^{\circ}C$ and $370^{\circ}C$ with stress ratio of R=0.1 and 0.3. The fatigue crack propagation rate da/dN related with the stress intensity factor range $\Omega\textrm{K}$ was influenced by the stress ratio within the stable growth of fatigue crack(Region II) with an increase in $\Omega\textrm{K}$. The resistance to the fatigue crack growth at high temperature is higher in comparison with that at room temperature, and the resistance attributed to the extent of plasticity-induced by compressive residual stress according to the cyclic loads. Fractographic examinations reveal that the differences of the fatigue crack growth characteristics between room and high temperatures are mainly explained by the crack closure and oxide-induced by high temperature.