• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2000.10a
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• pp.240-245
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• 2000

Fatigue crack propagation rates and characteristics of the SA516-60 steel which is used for the low temperature pressure vessels, were studied in the room temperature of $25^{\circ}C$ and low temperature ranges of $10^{\circ}C,\; -10^{\circ}C,\; -30^{\circ}C,\; -50^{\circ}C, \;and\; -70^{\circ}C4 with stress ratio of R=0.05. The obtained experimental results are as follows; 1) In the logarithmic relationship between the fatigue crack propagation rate(da/dN) and stress intensity factor K, the linear relationship was obtained up to da/dN 〉$8\times10^{-3}$/mm/cycle in the same of room temperature, but in low temperature case, the relationship was extended to the range of crack propagation rate. 2) The lower limit stress intensity factor of SA516-60 $\DeltaK_{th}$ was 15.8MPa and in the case of low temperature $-50^{\circ}C\; and\; -70^{\circ}C$, the crack propagation rate da/dN which showed a linear relation, reached rapidly to the $\DeltaK_{th}$/. As the results, the crack propagation rates of $-50^{\circ}C\; and\; -70^{\circ}C$ were lower than that of room temperature and according to the testing temperature the rates were decreased rapidly to the $\DeltaK_{th}$/. 3) On the relationship between the stress intensity factor $\DeltaK$ and the crack propagation cycle, the stress intensity factors of low cycle region was rapidly increased at low temperature, but $\DeltaK$ was increased rapidly at room temperature of high cycle. 4) On the relationship between the fatigue crack propagation rate and cycle, the fatigue crack propagation rate showed higher gradient in the room temperature than the low temperature due to the increment in ductility at low temperature.

• Journal of Mechanical Science and Technology
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• v.17 no.3
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• pp.343-349
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• 2003

The fuselage-wing intersection suffers from the cyclic bending moment of variable amplitude. Therefore, the influence of cyclic bending moment on the delamination and the fatigue crack propagation behavior in AFRP/Al laminate of fuselage-wing was investigated in this study. The cyclic bending moment fatigue test in AFRP/Al laminate was performed with five levels of bending moment. The shape and size of the delamination Lone formed along the fatigue crack between aluminum sheet and aramid fiber-adhesive layer were measured by an ultrasonic C-scan. The relationships between da/dN and ΔK, between the cyclic bending moment and the delamination zone size, and between the fiber bridging behavior and the delamination zone were studied. As results, fiber failures were not observed in the delamination zone in this study, the fiber bridging modification factor increases and the fatigue crack growth rate decrease and the shape of delamination zone is semi-elliptic with the contour decreasing non-linearly toward the crack tip.

• Journal of Life Science
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• v.9 no.6
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• pp.722-727
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• 1999

We carried out immunoblot analyses to study expression and subcellular distribution of the N-methyl-D-aspartate receptor(NR) subunits in salmon (Chum Salmon, Oncorhynchus keta). We prepared subcellular fractions such as brain homogenates, synaptosomes, and postsynaptic density (PSD) from salmon brains, and analyzed protein compositions by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). In a Coomassie-stained 6% SDS-gel, about 20 distinct major protein bands could be identified in the PSD fraction. Immunoblot analyses using antibodies against rat NR subunit 2A and 2B antigens (NR2A and NR2B, respectively) showed weak but evident signals at the 180 kDa positions in the salmon PSD fractions. However, in contrast to rat NRs, the salmon NR2A and NR2B are not recognized by a phosphotyrosine-specific antibody suggesting that the salmon NRs are regulated differently from those of the rat by protein tyrosine kinases. Our results indicate that NR2A and NR2B subunits are expressed in the salmon PSD fraction but not regulated by tyrosine phosphorylation.

• Journal of Information Processing Systems
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• v.7 no.4
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• pp.613-626
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• 2011

In this paper, we focus on the pinwheel task model with a variable voltage processor with d discrete voltage/speed levels. We propose an intra-task DVS algorithm, which constructs a minimum energy schedule for k tasks in O(d+k log k) time We also give an inter-task DVS algorithm with O(d+n log n) time, where n denotes the number of jobs. Previous approaches solve this problem by generating a canonical schedule beforehand and adjusting the tasks' speed in O(dn log n) or O($n^3$) time. However, the length of a canonical schedule depends on the hyper period of those task periods and is of exponential length in general. In our approach, the tasks with arbitrary periods are first transformed into harmonic periods and then profile their key features. Afterward, an optimal discrete voltage schedule can be computed directly from those features.

• Parasites, Hosts and Diseases
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• v.45 no.1 s.141
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• pp.19-26
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• 2007

This study describes the isolation of a Toxocara canis species-specific excretory-secretory(ES) antigen and the development of an enzyme-linked immunosorbent assay(ELISA) based on this antigen. Analysis of the ES antigens of T. canis, Toxocara vitulorum, Ascaris lumbricoides and Necator americanus larval antigen was performed by SDS-PAGE followed by western blotting. A 57 kDa T. canis-specific antibody fraction(TcES-57) was identified by western blotting and labelling with anti-Toxocara antibodies(from experimental rabbits and human patients) and tracing with anti-human or anti-rabbit peroxidase conjugate. No protein fraction of 57 kDa was detected in ES or larval antigens collected from T. canis, T. vitulorum, A. lumbricoides and N. americanus. Using TcES-57, a specific anti-serum was produced in rabbits and a double sandwich ELISA was developed. This test was validated using known seropositive sera from toxocariasis patients, sera from A. lumbricoides or N. americanus patients, and 50 serum samples from cats. These tests revealed that TcES-57 antigen is specific to T. canis infection and does not cross react with sera of other related infections. Thus, ELISA based on TcES-57 antigen was proven to be an effective tool in the diagnosis of toxocariasis and studies on the role of T. canis in the epidemiology of human toxocariasis.

• Journal of the Korean Society of Safety
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• v.5 no.1
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• pp.19-29
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• 1990

The objective of this paper is to investigate the effect of residual stresses on the $\Delta$K$\sub$th/ and fatigue crack growth behavior of butt weldments. For this purpose, transverse butt sutmerged arc welding was performed on SM50A steel plate and CT(compact tension) specimens which loading direction is perpendicular to weld bead were selected. Welding residual stresses distribution on the specimen was determined by hole drilling method. The case of crack located parallel to weld bead, the states of as weld and PWHT, $\Delta$K$\sub$th/ of specimens(HAZ, weld zone) was higher than that of the base metal probably because of the compressive residual stresses of crack tip. In low $\Delta$K region, it is estimated that the effects of residual stresses for da/dN are great. In region II, the da/dN of weldments in as weld state was lower than that of the base metal. Though da/dN of Weldments in PWHT state was similar to that of the base metal. The constant of power law, m in two states consisted with the base metal. Therefore , it is estimated that the value of m is not affected by residual stresses. Fatigue crack growth behavior of weldments consisted with the base metal considering the effective stress intensity factor range($\Delta$K$\sub$eff/) included the effect of initial residual stress(Kres). Thus, we can predict the fatigue crack growth behavior of weldment by knowing the distribution of initial residual stress at the crack tip.

• BMB Reports
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• v.31 no.5
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• pp.453-458
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• 1998

Human thrombopoietin (hTPO) was expressed to high levels in insect cells using the baculovirus expression system. Full-length hTPO cDNA containing a native signal peptide sequence was amplified by PCR from a human fetal liver cDNA library and cloned into the Autographa californica nuclear polyhedrosis virus (AcNPV) expression vector. Immunoblot analysis with antiserum against hTPO indicated that an approximately 55 kDa protein was produced in recombinant AcNPV infected insect cells. Recombinant hTPO was produced 4-fold higher in Trichoplusia ni (Tn5) cells than in Spodoptera frugiperda (Sf9) cells. with most of the hTPO produced in Tn5 cells secreted into the culture medium. Addition of tunicamycin in the culture medium resulted in the reduction of the size of hTPO to 35-38 kDa, and most of the protein remained within the cell. These results suggest that N-glycosylation of hTPO is required for the secretion of the protein into the culture medium in insect cells. hTPO produced in insect cells induced proliferation and maturation of megakaryocyte progenitors, indicating that it is in a biologically active form.

• Journal of Ocean Engineering and Technology
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• v.13 no.3 s.33
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• pp.108-113
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• 1999

In this study, CT specimens were prepared from ASTM A516 Gr. 65 which was used for pressure vessel plates for moderate and lower temperature service. Fatigue crack growth test was carried out in the environment of low temperature of $10^{circ}C, -10^{circ}C, -30${circ}C\;and\;-50^{circ}C$ and in the range of stress ratio of R=0.05 and 0.3 by means of opening mode displacement. Based on these test results, the characteristics from temperature and stress ration were shown as follows. 1) As the stress ratio, R increased da/dN and ${Delta}K$ of 2nd stage gradually decreased. And as R decreased, the effect of temperature became greater and greater. 2) As the temperature descended, da/dN decreased on a certain ${Delta}K$, and ${Delta}K$ did in a same da/dN. And the stress ratio, R exerted greater influence at the lower temperature. 3) The fatigue crack growth constant, m increased at $10^{circ}C$ and $-10^{circ}C$, snd decreased at $-30^{circ}C$ and $-50^{circ}C$ following the increment of stress ratio R. And m increased along with the reduction of temperature greatly decreased at $-30^{circ}C$ to come close to two(2).

• BMB Reports
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• v.47 no.5
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• pp.256-261
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• 2014

To investigate the function of N-glycosylation of Cel5A (endoglucanase II) from Hypocrea jecorina, two N-glycosylation site deletion Cel5A mutants (rN124D and rN124H) were expressed in Saccharomyces cerevisiae. The weights of these recombinant mutants were 54 kDa, which were lower than that of rCel5A. This result was expected to be attributed to deglycosylation. The enzyme activity of rN124H was greatly reduced to 60.6% compared with rCel5A, whereas rN124D showed slightly lower activity (10%) than that of rCel5A. rN124D and rN124H showed different thermal stabilities compared with the glycosylated rCel5A, especially at lower pH value. Thermal stabilities were reduced and improved for rN124D and rN124H, respectively. Circular dichroism spectroscopy showed that the modification of secondary structure by mutation may be the reason for the change in enzymatic activity and thermal stability.

• The Korean Journal of Pharmacology
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• v.29 no.2
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• pp.275-282
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• 1993

Liver microsomal epoxide hydrolase (mEH) is active in the detoxification of epoxide-containing reactive intermediate. Previous studies in this laboratory have shown that thiazole and pyrazine are efficacious inducers of mEH in rats with large increases in mEH mRNA levels (Carcinogensis, Kim et al, 1993). mEH was purified to electrophoretic homogeneity from thiazole-induced rat hepatic microsomes using DEAE-cellulose column chromatography whereas another protein $({\sim}43\;kDa)$ was co-purified with mEH from pyrazine-induced rat hepatic micrsomes (200 mg/kg body weight/day, ip, 3d). The antibody raised from a rabbit against mEH protein purified from thiazole-induced rat hepatic microsomes appeared to specifically recognize mEH protein in rat hepatic microsomes, as assessed by immunoblotting analysis. Immunoblotting analyses revealed a 10- and 7-fold increase in mEH levels in the hepatic microsomes isolated from thiazole- and pyrazine-treated rats, respectively. Moreover, immunoblotting analysis showed cross-reactivity of the mEH antibody with a 43 kDa protein in pyrazine-induced rat hepatic microsomes and with co-purified 43 kDa protein in purified fractions. The ratio between the 43 kDa protein and mEH in pyrazine-induced rat microsomes or in purified fractions was ${\sim}1$ to 15. N-terminal amino acid sequence analysis of both purified rat mEH and 43 kDa protein revealed that 10 out of 12 amino acids in N-terminus of the 43 kDa protein were identical with the mEH sequence with two amino acid residues of the 43 kDa protein undetermined. Either thiazole or pyrazine treatment, however, failed to increase the levels of mEH protein in rabbits while pyrazine caused elevation of the 43 kDa protein in this species, as determined by irnrnunoblotting analysis. These results demonstrated that treatment of rats with either thiazole or pyrazine causes elevation in hepatic mEH expiession whereas pyrazine treatment results in induction of another mEH-related 43 kDa protein and that a distinct species difference exists between rats and rabbits in the induction of mEH by these xenobiotics.