• Korean Journal of Microbiology
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• v.38 no.2
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• pp.67-73
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• 2002

The cellular responses of soil-borne bacterium, Pseudomonas sp. HK-6 to explosive 2,4,6-trinitrotoluene (TNT) were examined. Two stress shock proteins (SSPs), approximately 70-kDa DnaK and a 60-kDa GroEL were found in HK-6 cells in response to TNT. Analyses of SDS-PAGE and Western blot using anti-DnaK and GroEL revealed that SSPs were induced in HK-6 cells exposed to 0.5 M of TNT far 6-12 hrs. The maximum induction of proteins was achieved at 8-hr incubation point after HK-6 cells'exposure to TNT. Similar SSPs were found to be induced in HK-6 cells by heat shock (shift of temperature, from $30^{\circ}C$ to $42^{\circ}C$) or cold shock (shift of temperature,$30^{\circ}C$ to $4^{\circ}C$).2D-PAGE of soluble protein tractions from the culture of Pseudomonas sp. HX-6 exposed to TNT demonstrated that approximately 450 spots were observed on the silver stained gels ranging from pH 3 to pH 10. Among them, 12 spots significantly induced and expressed in response to TNT were selected and analyzed. Approximately 60-kDa protein, which was assumed highly expressed on the gel, was used for amino acid sequencing. N-terminal microsequencing with in-gel digestion showed that N-terminal sequence of the TNT-induced protein, <$^1XXAKDVKFGDSARKKML^17$, shared extensive similarity with $^1XXAKDVKFGDSARKKML^17$, N-terminal sequence of (P48216) GroEL of Pseudomonas putida.

• Transactions of the Korean Society of Mechanical Engineers
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• v.10 no.1
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• pp.62-69
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• 1986

Fatigue surface crack growth was studied in 7075-T651 aluminum alloy plates subjected largely to bending loads. The surface crack length and its depth were measurement by the unloading elastic compliance method. The surface crack growth rate dc/dN, on the surface and da/dN, in the depth direction were obtained by the secant method. The stress intensity factor range .DELTA.K was computed by means of Newman and Raju equation. The aspect ratio a/c was presented in form of a/c=0.815-0.853(a/T). The effect of the stress ratio on the stable surface crack growth rates under increasing .DELTA.T is larger in lower .DELTA.K, while the relation between dc/dN, da/dN and the effective stress intensity factor range .DELTA.K$_{eff}$ is weakly dependent on the stress ratio.o.

• Korean Journal of Biological Psychiatry
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• v.3 no.1
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• pp.51-56
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• 1996

The effects of 3 week treatment with haloperidol(2mg/kg/day, i.p.) on dopamine(DA) D2 receptor and DA metabolism in rat striata were studied at various time points after withdrawal from the drug treatment. Striatal DA D2 receptors were characterized with the radioligand 0.5nM [$^3H$]Spiperone. Dopamine(DA), homovanillic acid(HVA), 3,4-dihydroxyphenyl acetic acid(DOPAC) in rat striatum were measured with the high performance liquid chromatography. Drug withdrawal for 1 week induced significant increase in the number of D2 receptor in striatum after withdrawal for 1 week(p<0.05), and then this change was restored to control level during the withdrawal time of 2 and 4 weeks. There was no difference in striatal concentrations of DA and its metabolites among the groups. In conclusion, one-week withdrawal from chronic haloperidol treatment induced DA D2 receptor supersensitivity in the striatum, and that was normalized rapidly. Though this adaptive change in DA receptors, it may not affect the metabolism of DA in striatum.

• Transactions of the Korean Society of Mechanical Engineers
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• v.12 no.5
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• pp.1097-1103
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• 1988

This paper deals with surface crack behavior and the fatigue life prediction of notched specimens using the relation between surface crack length, a, and the cycle ratio, $N/N_{f}$. From the $a-N\;/\;N_{f}$ curves, UC(the upper limit curve), LC(the lower limit curve) and MC(the middle limit curve) were assumed and utilized to predict the fatigue life and crack growth rate. The data computed from the three assumed curves were compared with the experimental data. It has been found that in the stable crack growth region ($N/N_{f}=0.3-0.8$) fatigue life can be predicted within 20% errors. Using the characteristics of $a-N\;/\;N_{f}$ curve, it is possible to predict the $da/dN-K_{max}$ curve, the $da/dN-{\Delta}K_{{\varepsilon}_t}$ curve, and the $S-N_{f}$ curve.

• The Korean Journal of Nuclear Medicine
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• v.35 no.4
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• pp.258-267
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• 2001

Purpose/Methods: Repeated administration of methamphetamine (METH) produces high extracellular levels of dopamine (DA) and subsequent striatal DA terminal damage. The effect of MK-801, a noncompetitive N-methyl-D-aspartate receptor antagonist, on METH-induced changes in DA transporter (DAT) and DA release evoked by an acute METH challenge was evaluated in rodent striatum uslng $[^3H]$]WIN 38,428 ex vivo auto-radiography and in vivo microdialysis. Results: Four injections of METH (10 mg/kg, i.p.), each given 2 h apart, produced 71% decrease in DAT levels in mouse striatum 3 d after administration. Pretreatment with MK-801 (2.5 mg/kg, i.p.) 15 min before each of the four METH injections protected completely against striatal DAT depletions. Four injections of MK-801 alone did not significantly change striatal DAT levels. Striatal DA release evoked by an acute METH challenge (4 mg/kg, i.p.) at 3 d after repeated administration of METH in rats was decreased but significant compared with controls, which was attenuated by repeated pretreatment with MK-801. Also, repeated injections of MK-801 alone attenuated acute METH-induced striatal DA release 3 d after administration. Conclusion: These results suggest that repeated administration of MK-801 may exert a preventive effect against METH-induced DA terminal injury through long-term attenuation of DA release induced by METH and other stimuli.

• Journal of Power System Engineering
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• v.3 no.2
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• pp.51-56
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• 1999

Fatigue crack propagation rates and characteristics of the SA516-70 steel which is used for the low temperature pressure vessels, were studied in the room temperature of $10^{\circ}C$ and low temperature ranges of $-10^{\circ}C,\;-30^{\circ}C,\;-50^{\circ}C,\;and\;-70^{\circ}C$ with stress ratio of R=0.05. The obtained experimental results are as follows; 1) In the logarithmic relationship between the fatigue crack propagation rate(da/dN) and stress intensity factor K, the linear relationship was obtained up to da/dN > $8{\times}10^3$ mm/cycle in the same of room temperature, but in low temperature case, the relationship was extended to the range of low crack propagation rate. 2) The lower limit stress intensity factor of SA516-70 ${\Delta}K_{th}\;was\;23MPa\sqrt{m}$ and in the case of low temperature $-50^{\circ}C\;and\;-70^{\circ}C$, the crack propagation rate da/dN which showed a linear relation, reached rapidly to the ${\Delta}K_{th}$. As the results, the crack propagation rates of $-50^{\circ}C\;and\;-70^{\circ}C$ were lower than that of room temperature and according to the testing temperature the rates were decreased rapidly to the ${\Delta}K_{th}$. 3) On the relationship between the stress intensity factor ${\Delta}K$ and the track propagation cycle, the stress intensity factors of low cycle region was rapidly increased at low temperature, but ${\Delta}K$ was increased rapidly at room temperature of high cycle. 4) On the relationship between the fatigue crack propagation rate and cycle, the fatigue crack propagation rate showed higher gradient in the room temperature than the low temperature due to the increment in ductility at low temperature.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.28 no.2
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• pp.216-227
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• 1992

The corrosion rate, behavior of corrosion fatigue and characteristic of cathodic protection for SB41 were investigated by corrosion and corrosion control tests in seawater at laboratory and coast. The main result obtained are as the following; 1) The corrosion rate of base metal (BM) is about 28-37 mg/dm super(2) day in seawater of coast. 2) The correlation between the stress intensity factor range $\Delta$K and crack propagation rate da/dN for weldment follows paris' rule in seawater : da/dN=C($\Delta$K) super(m) where m is the slope of the correlation, and is 2.02 for BM and 1.75 for heat affected zone (HAZ) respectively. 3) The corrosion sensitivity of HAZ is more sensitive than that of BM under the low region of $\Delta$K. 4) With increase of bared surace area of cathode, cathodic protection potential is increased sharply.

• Journal of Ocean Engineering and Technology
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• v.1 no.2
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• pp.83-92
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• 1987

Inorder ot estimate the running life of turbine rotors, fatigue crack propagation low, da/dN = C(${\DELTA}K)^m$, proposed by paris et al. has been widely applied. In this study, fatigue crack propagation rates for 16 samples of 1% Cr-Mo-V rotor steel were measured and statistical characteristics of m and C values in above equation were reviewed. The results are summarized as follows. 1. C and m follow a log-normal distribution and normal distribution, respectively. And the relation of C and shows a strong negative correlation. 2. Fatigue crack propagation equation can be expressed as da/dN=$4.11{\times}10^{-4}({\Delta}K/153.8)^m$, introducing the ralationship C=$C_oK_o^{-m}$. In this case, contribution of $C_o$ distribution to the distribution of log C shows very small compared to degrees of contribution by m.

• Transactions of the Korean Society of Mechanical Engineers
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• v.16 no.9
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• pp.1681-1690
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• 1992

The effects of the stress ratio and the fiber orientation(0.deg./90.deg. and .+-.45.deg.) to the load direction on the fracture behavior of the glass/epoxy plain woven composites were studied. The tests were carried out using compact tension specimens under both static and fatigue loading. The values of $k_{a}$ obtained from the energy release rate are independent of notch depth(a/w=0.2~0.6) for the 0.deg./90.deg. specimens, but decreases with an increase in a/w for the .+-.45.deg. specimens. And $k_{q}$ has higher values than $k_{ASTM}$ has been evaluated by the ASTM E399 test procedure. It is shown in the relation between fatigue crack growth rate da/dN and stress intensity factor range .DELTA.K using modified shape correction factor that da/dN decreases with a decrease in stress ratio and is lower for .+-..deg. specimens than for 0.deg./90.deg. These phenomena can be explained by the crack deflection to the load direction.n.n.

• Journal of Life Science
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• v.14 no.4
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• pp.636-643
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• 2004

D-Xylose isomerase produced by Lactococcus sp. JK-8, isolated from kimchi, was purified 17-fold of homogeneity, and its physicochemical properties were determined. Although the N-terminal amino acid sequence of D-xylose isomerase was analysed to Ala-Tyr-Phe-Asn-Asp-Ile-Ala-Pro-Ile-Lys, it was not similar to that of Lactobacillus enzyme. The molecular weight of the purified enzyme was estimated to be 180 kDa by gel filtration, 45 kDa by SDS-PAGE and the enzyme was homotetramer. The optimum pH of the enzyme was around 7 and stable between pH 6 and 8. The optimum reaction temperature was 7$0^{\circ}C$ and stable up to 7$0^{\circ}C$ in the presence of 1 mM $Mn^{2+}$. Like other D-xylose isomerases, this enzyme required divalent cation, such as $Mg^{2+}$, $Co^{2+}$, or $Mn^{2+}$ for the activity and thermostability. $Mn^{2+}$was the best activator. Substrate specificity studies showed that this enzyme was highly active on D-xylose. The enzyme had an isoelectric point of 4.8, and fm values for D-xylose was 5.9 mM.