• International Journal of Precision Engineering and Manufacturing
• /
• v.5 no.4
• /
• pp.36-42
• /
• 2004

The shaft with the circular cross section has symmetric structural combination parts to keep the rotating balance. Hence the crack usually initiates from symmetric combination parts due to the stress concentration of these parts. In this study to estimate the fatigue behavior of symmetric cracks, the fatigue test was performed by using a rotary bending tester and the specimen with symmetric defects in circular cross section. The characteristics of crack initiation and propagation on the symmetric surface cracks in circular cross section were examined. We also observed the internal crack using the oxidation coloring method and investigated the fatigue behavior using the relationship between the surface crack and the internal crack. As a result, the fatigue life of symmetric cracks was reduced by 35% compared to that of a single crack. We examined the characteristics of fatigue behavior of elements with symmetric cracks using internal crack propagation rate and maximum stress intensity factor range that were obtained from an approximation method.

• Animal cells and systems
• /
• v.6 no.4
• /
• pp.341-347
• /
• 2002

The life cycle of Acanthamoeba is comprised of two distinct stages, tropho-zoite and cyst. During periods of stress, trophozoites undergo cellular differentiation into cyst. In order to understand the cellular differentiation, ore followed changes in profiles of major proteins by 2D-PAGE and ubiqui-tinated proteins by immunoblotting with anti-ubiquitin (Ub) monoclonal antibody (mAb) as a probe. We observed 51 proteins present in trophozoite were lost with the encystment. We found that 43 proteins within 24 h, and 8 proteins in 96 h of encystment. Among them, 17 proteins were staine with anti-Ub mAb. In cysts, 16 proteins including 2 anti-Ub mAb-reactive proteins were newly synthesized. Four proteins were newly detected in 24 h-cyst and disappeared in 96 h-cyst, one protein was synthesized in 24-96 h-cyst and disappeared in 168 h-cyst, and 11 proteins appeared upon en-cystment and were present in all cyst stages. We identified a cyst specific 33 kDa protein as subtilisin-like serine proteinase by N-terminal sequencing. Identification of these proteins lost and newly synthesized with encystment would improve our understanding of cysting protozoan parasites.

• Archives of Pharmacal Research
• /
• v.27 no.1
• /
• pp.118-126
• /
• 2004

The aqueous extract of European mistletoe (Viscum album, L.) has been used in cancer therapy. The purified mistletoe lectins, main components of mistletoe, have demonstrated cytotoxic and immune-system-stimulating activities. Korean mistletoe (Viscum album L. coloratum), a subspecies of European mistletoe, has also been reported to possess anticancer and immunological activities. A galactose- and N-acetyl-D-galactosamine-specific lectin (Viscum album L. coloratum agglutinin, VCA) with Mr 60 kDa was isolated from Korean mistletoe. Mistletoe preparations have been given subcutaneously due to the low stability of lectin in the gastrointestinal (GI) tract. In the present study, we investigated the possibility of alginate/chitosan microcapsules as a tool for oral delivery of mistletoe lectin. In addition, our strategy has been to develop a system composed of stabilizing cores (granules), which contain mistletoe lectin, extract or powder, coated by a biodegradable polymer wall. Our results indicated that successful incorporation of VCA into alginate/chitosan microcapsules has been achieved and that the alginate/chitosan microcapsule protected the VCA from degradation at acidic pH values. And coating the VCA with polyacrylic polymers, Eudragit, produced outstanding results with ideal release profiles and only minimal losses of cytotoxicity after manufacturing step. The granules prepared with extract or whole plant produced the best results due to the stability in the extract or whole plant during manufacturing process.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.16 no.8
• /
• pp.1493-1502
• /
• 1992

The purpose of this study is to identify the effects of loading variables on fatigue crack opening behavior, using structural steel SS41. To use various stress ratios, three kinds of the methods varying stress ratio were used; .DELTA.P const., P$_{max}$ const., and P$_{min}$ const.. To measure the opening load, the direct measuring method which measures the distance between two micro indentations 20 .mu.m behind crack tip and the elastic compliance method were used. As the results of present study, the following conclusions are obtained. First, using the direct measuring method, it was possible to measure the COD at any location behind crack tip. Second, as measuring point becomes farther from crack tip, opening load becomes smaller. Third, the acceleration of da/dN near notch is due to crack opening behavior. Finally, opening ratio is a function of not only R, but .DELTA.K.K.K.

• Journal of Microbiology and Biotechnology
• /
• v.8 no.3
• /
• pp.270-276
• /
• 1998

Extracellular ${\alpha}$-glucosidase was purified to homogeneity from moderately thermophilic Bacillus sp. DG0303. The thermostable ${\alpha}$-glucosidase was purified by ammonium sulfate fractionation, ion-exchange chromatography, preparative polyacrylamide gel electrophoresis (PAGE), and electroelution. The molecular weight of the enzyme was estimated to be 60 kDa by SDS-PAGE. The optimum temperature for the action of the enzyme was at $60^{\circ}C$. It had a half-life of 35 min at $60^{\circ}C$. The enzyme was stable at the pH range of 4.5~7.0 and had an optimum pH at 5.0. The enzyme preparation did not require any metal ion for activity. The thermostable ${\alpha}$-glucosidase hydrolyzed the ${\alpha}$-1,6-linkages in isomaltose, isomaltotriose, and panose, and had little or no activity with maltooligosaccharides and other polysaccharides. The $K_m$ (mM) for p-nitrophenyl-${\alpha}$-D-glucopyranoside (pNPG), panose, isomaltose, and isomaltotriose were 4.6, 4.7, 40.8, and 3.7 and the $V_{max}$(${\mu}mol{\cdot}min^-1$$mg^-1$) for those substrates were 5629, 1669, 3410, and 1827, respectively. The N-terminal amino acid sequence of the enzyme was MERVWWKKAV. Based on its substrate specificity and catalytic properties, the enzyme has been assigned to be an oligo-1,6-glucosidase.

• Journal of the Korean Society for Precision Engineering
• /
• v.16 no.8
• /
• pp.149-154
• /
• 1999

FRMLs(Fiber Reinforced Metal Laminates) is a new type of hybrid materials. FRMLs consists of high strength metal and fiber which are laminated using a structural adhesive bond(epoxy resin). The effect of resin mixture ratios on the fatigue crack propagation behavior and mechanical property of Aramid fiber reinforced aluminum composites was investigated. The epoxy, diglycidylether of bisphenol A(DGEBA) was cured with methylene dianiline(MDA) with or without accelerator(K-54). Eight kinds of resin mixture ratio were tested for the experiment ; five kinds of FRMLs(1))epoxy & curing agent) and three kinds of FRMLs(2)(epoxy & curing agent & accelerator). FRMLs(2) have a more effective characteristics on the fatigue crack propagation behavior and mechanical property than FRMLs(1)

• Journal of Microbiology and Biotechnology
• /
• v.20 no.12
• /
• pp.1756-1763
• /
• 2010

Pasteurella multocida serogroup D strain, which produces P. multocida toxin (PMT), is a widespread and harmful pathogen of respiratory diseases such as pneumonia and progressive atrophic rhinitis (PAR) in swine. Vaccination has been considered the most desirable and effective approach for controlling the diseases caused by toxigenic P. multocida. To investigate the antigenicity and immunogenicity of partial fragments of recombinant PMT, recombinant proteins of the N-terminal (PMT-A), middle (PMT-B), C-terminal (PMT-C), and middle-C-terminal (PMT2.3) regions of PMT were successfully produced in an Escherichia coli expression system. The molecular masses of PMT-A, PMT-B, PMT-C, and PMT2.3 were ca. 53, 55, 35, and 84 kDa, respectively, purified by nickel-nitrilotriacetic acid (Ni-NTA) affinity column chromatography. All the recombinant proteins except for PMT-A showed immune responses to antisera obtained from a swine showing symptoms of PAR. Moreover, high titers of PMT-specific antibodies were raised from mice immunized with each of the recombinant proteins; however, the immunoreactivities of the antibodies to authentic PMT and heat-inactivated whole bacteria were different, respectively. In the protection study, the highest protection against homologous challenge was shown in the case of PMT2.3; relatively poor protections occurred for the other PMT fragments.

• Journal of Microbiology and Biotechnology
• /
• v.26 no.6
• /
• pp.1087-1097
• /
• 2016

An intracellular lipase from Anoxybacillus flavithermus HBB 134 was purified to 7.4-fold. The molecular mass of the enzyme was found to be about 64 kDa. The maximum activity of the enzyme was at pH 9.0 and 50℃. The enzyme was stable between pH 6.0 and 11.0 at 25℃, 40℃, and 50℃ for 24 h. The K_m and V_max of the enzyme for pNPL substrate were determined as 0.084 mM and 500 U/mg, respectively. Glycerol, sorbitol, and mannitol enhanced the enzyme thermostability. The enzyme was found to be highly stable against acetone, ethyl acetate, and diethyl ether. The presence of PMSF, NBS, DTT and β-mercaptoethanol inhibited the enzyme activity. Hg²⁺, Fe³⁺, Pb²⁺, Al³⁺, and Zn²⁺ strongly inhibited the enzyme whereas Li⁺, Na⁺, K⁺, and NH₄⁺ slightly activated it. At least 60% of the enzyme activity and stability were retained against sodium deoxycholate, sodium taurocholate, n-octyl-β-D-glucopyranoside, and CHAPS. The presence of 1% Triton X-100 caused about 34% increase in the enzyme activity. The enzyme is thought to be a true lipase since it has preferred the long-chain triacylglycerols. The lipase of HBB 134 cleaved triolein at the 1- or 3-position.

• Journal of Welding and Joining
• /
• v.7 no.1
• /
• pp.51-58
• /
• 1989

The purpose of this study is to investigate the corrosion fatigue crack growth of PWTHT specimens(SS41, SM53B) which are the compact tension ones extracted from the muti-passed weldment and weld block. The corrosion fatigue test was done at the cyclic stress frequency of 3Hz in 3.5% NaCl solution. The results are as follows. 1. Corrosion fatigue crack growth of as-weld was slower than that of base metal. 2. In the low .DELTA.K region, the effect of corrosion environment on crack growth was obvious. However, the corrosion effect decreased with the .DELTA.K slowly. 3. The behaviour of fatigue and corrosion fatigue crack growth depended on the material, heat treatment as well as experimental conditions. 4. Corrosion fatigue crack growth of PWHT specimens(SS41, SM53B) subjected to 1/4hr, was increased compared with that of as-weld. 5. There was a tendency that the exponent value(m) obtained in 3.5% NaCl solution was decreased in comparison with that in air, and the material constant(C)was increased for Paris equation, da/dN=C((.DELTA.K))$^{m}$ , compared with that in air considerably.

• Journal of the Institute of Electronics Engineers of Korea TC
• /
• v.39 no.1
• /
• pp.20-33
• /
• 2002

Usage of ATM AAL2 packets becomes dominant to increase transmission efficiency of voice traffic in the backbone network. In case of voice service that uses AAL2 mechanism, if resources of network are enough, connection of new call is accepted. However, due to packets generated by the new call, transmission delay of packets from old calls can increase sharply. To control this behavior, in this paper we present an AAL2 buffer management scheme that allocates a virtual buffer to each call and after calculating its propagation delay variation(PDV), decides to drop packets coming from each call according to the PDV value. We show that this packet dropping algorithm can effectively prevent abrupt QoS degradation of old calls. To do this, we analyze AAL2 packet composition process to find a critical factor in the process that influences the end-to-end delay behavior and model the process by K-policy M/D/1 queueing system and MIN(K, Tc)-policy M/D/1 queueing system. From the mathematical model, we derive the probability generating function of AAL2 packets in the buffer and mean waiting time of packets in the AAL2 buffer. Analytical results show that the AAL2 packet dropping algorithm can provide stable AAL2 packetization delay and ATM cell generation time even if the number of voice sources increases dramatically. Finally we compare the analytical result to simulation data obtained by using the COMNET Ⅲ package.