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Evaluation of Genome Based Estimated Breeding Values for Meat Quality in a Berkshire Population Using High Density Single Nucleotide Polymorphism Chips

  • Baby, S.;Hyeong, K.E.;Lee, Y.M.;Jung, J.H.;Oh, D.Y.;Nam, K.C.;Kim, T.H.;Lee, H.K.;Kim, Jong-Joo
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.11
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    • pp.1540-1547
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    • 2014
  • The accuracy of genomic estimated breeding values (GEBV) was evaluated for sixteen meat quality traits in a Berkshire population (n = 1,191) that was collected from Dasan breeding farm, Namwon, Korea. The animals were genotyped with the Illumina porcine 62 K single nucleotide polymorphism (SNP) bead chips, in which a set of 36,605 SNPs were available after quality control tests. Two methods were applied to evaluate GEBV accuracies, i.e. genome based linear unbiased prediction method (GBLUP) and Bayes B, using ASREML 3.0 and Gensel 4.0 software, respectively. The traits composed different sets of training (both genotypes and phenotypes) and testing (genotypes only) data. Under the GBLUP model, the GEBV accuracies for the training data ranged from $0.42{\pm}0.08$ for collagen to $0.75{\pm}0.02$ for water holding capacity with an average of $0.65{\pm}0.04$ across all the traits. Under the Bayes B model, the GEBV accuracy ranged from $0.10{\pm}0.14$ for National Pork Producers Council (NPCC) marbling score to $0.76{\pm}0.04$ for drip loss, with an average of $0.49{\pm}0.10$. For the testing samples, the GEBV accuracy had an average of $0.46{\pm}0.10$ under the GBLUP model, ranging from $0.20{\pm}0.18$ for protein to $0.65{\pm}0.06$ for drip loss. Under the Bayes B model, the GEBV accuracy ranged from $0.04{\pm}0.09$ for NPCC marbling score to $0.72{\pm}0.05$ for drip loss with an average of $0.38{\pm}0.13$. The GEBV accuracy increased with the size of the training data and heritability. In general, the GEBV accuracies under the Bayes B model were lower than under the GBLUP model, especially when the training sample size was small. Our results suggest that a much greater training sample size is needed to get better GEBV accuracies for the testing samples.

Variation of Discharge in Length of Hard Micro-path of Drip Hose (점적 호스의 경질 미로 길이에 대한 유량의 변화)

  • Kim, J.H.;Woo, M.H.;Song, N.S.;Kim, D.E.
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2017.04a
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    • pp.57-57
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    • 2017
  • Water line은 UV 처리된 호스의 내부에 압력보상 기능이 첨부된 경질의 미로 튜브를 끼워 사용하도록 제작되어 있다. 점적 호스의 외경은 15.8mm이며 대부분 0.5~2.5bar 사이의 압력에 1.5~3.0 L/hr의 유량을 공급한다. 따라서 전적 호스의 내부에 위치한 경질 미로 튜브의 설계는 유량의 압력보상에 밀접한 관계가 있다. 본 연구에서는 경질 미로 튜브를 통과하는 작은 돌기의 수가 압력과 유량에 어떤 영향을 미치는지에 대한 기초적인 실험을 수행하였으며 경질 미로의 압력과 유량 특성에 따른 이론적 해석과의 비교분석을 통하여 새로운 미로의 형상 설계에 활용할 기초적 연구를 수행하고자 하였다. 점적 호스의 성능은 길이(보통 300~400m)에 대한 유량의 균등성을 유지하는 것이며 균등성은 압력보상에 의해 이루어진다. 원통형 미로는 점적 호스의 내부에 삽입되어 원통 부분에 형성된 미로의 길이에 따른 유량의 균등성을 유지하도록 설계되어 있다. 점적 호스의 원통 주변에는 8개의 미로로 구성되어 있으며 미로의 길이를 금속 평면으로 금형 제작하여 투명판에서 미로의 형상을 보이게 하였다. 또한 입구부분에 물을 공급하고 미로의 각 위치별 배출구를 설치하여 압력에 따른 유출량을 전자저울에 측정하여 압력별 유량의 균등성에 대한 데이터를 측정하였다. 유입구의 압력을 0.5, 1.5, 2.5, 3.0bar로 각각 공급하였으며 유출량은 미로의 8개 구간에 따라 측정하였다. 경질 미로의 유출량은 미로의 길이가 짧을수록, 압력이 커질수록 유출량이 높게 나타났다. 또한 미로의 길이가 짧을 경우에는 유출량의 차이가 비교적 크게 나타났으나 미로의 길이가 길수록 압력에 대한 유출량에 대한 차이가 크지 않음을 알 수 있었다. 따라서 경질 미로에서 미로의 길이를 길게 할 경우에는 유출량을 비교적 균일하게 할 수 있음을 보여주고 있다. 반면에 미로의 길이가 길어질수록 압력변화에 따른 유출량이 지수적으로 감소함을 나타내고 있다. 미로의 길이가 7번과 8번에서는 상당히 균일한 성능을 보여주므로 경질 미로의 배출구를 전체적으로 모두 활용함으로써 압력보상을 비교적 균일하게 할 수 있음을 보여주고 있다. 압력보상은 탄성을 가진 실리콘과 미로로 구성될 경우에 가장 큰 효과를 나타내지만 점적 호스와 같이 경질 미로만으로 압력보상을 해야 할 경우에는 경질 미로의 길이가 매우 중요하다.

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Study on Efficiency of Silicon in PC Dripper of Drip Irrigation (점적관수기의 PC 드립용 실리콘 특성에 관한 연구)

  • Kim, J.H.;Choi, J.M.;Song, N.S.;Kim, D.E.
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2017.04a
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    • pp.56-56
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    • 2017
  • 점적 호스 중 PC 드립은 경질 호스의 내부에 Micro-path가 부착된 유로장치와 압력을 보상하는 연질의 실리콘으로 구성되어 있다. 이때 일정압력에서 적정 길이까지 동시에 내부 압력을 유지하며 균등하게 유출구를 통하여 유량을 공급하는 압력보상 기능이 매우 중요하다. 그러나 이 부분에 대한 기술은 선진국에 비해 아직 부족하여 수입에 의존하는 경향이 매우 높다. 뿐만 아니라 선진국 제품에 비해 유로장치 및 연질 실리콘의 탄성이 균일하지 못해 압력에 따른 유량의 편차가 큰 편이다. 본 연구에서는 우리나라 제품의 PC 드립 압력 균등성 기술을 향상시키기 위해 PC 드립의 핵심기술인 연질 실리콘의 성능에 대한 평가와 유량의 편차를 분석하여 문제점을 통한 개선방안을 제시하고자 하였다. 연질 실리콘의 규격은 $17{\times}8{\times}0.85mm$ (가로${\times}$세로${\times}$두께)로서 Water line의 내부에 결합되어 구성된다. PC 드립용 실리콘은 국내 A사 제품을 중심으로 하여 성능을 측정하였다. 측정을 정밀하게 하기 위해 Microtester를 사용하여 일정하게 하중이 부과되도록 하였다. 연질의 실리콘의 경도는 압력 보상에 중요한 기능을 하고 있으며 또한 PC 드립의 경질 미로와 같이 압력을 제어하는 역할을 하고 있다. 국내 A사의 실리콘 경도는 이상적인 실리콘 경도에 비하면 매우 불균일하였다. 세부적으로는 40 이하의 낮은 경도에서는 비교적 일치하였으나 경도 45 이상에서는 큰 차이를 보여주고 있었다. 국내 A사의 실리콘 경도에 따른 공급유량의 특성에서는 실리콘의 경도가 낮을수록 실리콘은 무르며 탄성이 줄어든다. 반면에 경도가 높을수록 탄성이 커지고 딱딱한 특성을 가진다. 동일한 PC 드립에 실리콘의 경도별 특성은 경도에 따른 유량이 반드시 일정하지 않으며 대체로 경도가 높을수록 동일 압력에 대해 유량이 많아지는 것을 알 수 있었다. 압력의 변화에 대해 유량이 일정하게 유지하는 특성을 가질수록 사용범위가 넓어지고 안정성이 높은 것으로 판단되며 실리콘 경도 35~55 범위에서 1~2bar의 공급압력에서 사용하는 것이 적합할 것으로 보여진다. 경도 45의 실리콘을 사용하여 수입 드립, 국내 A사의 드립 및 수입과 국내 혼용시 압력에 따른 공급유량의 특성을 분석하였다. 최대 유량과 최소 유량에서 발생되는 오차는 수입 실리콘이 비교적 작았으나 3.6 bar 이상에서는 유량이 급격히 떨어지는 양상을 보였다. 국내 A사의 제품은 0.8~2.0 bar 까지 성능이 비슷했으나 0.8 bar 이하에서는 오차가 있었으나 높은 압력에서는 오히려 성능이 우수하였다. 수입 드립과 다소 유사한 성능을 나타낸 것이 A사 드립과 수입 실리콘을 혼용하여 사용한 것은 전체적으로 비슷한 양상을 나타내었다. 따라서 실리콘 경도 오차를 고려하면 압력별 유량의 변화는 실리콘의 경도와 밀접한 관계가 있는 것으로 판단되었다.

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Biomechanical Analysisz of Varying Backpack Loads on the Lower Limb Moving during Downhill Walking (내림 경사로 보행시 배낭 무게에 따른 하지 움직임의 운동역학적 분석)

  • Chae, Woen-Sik;Lee, Haeng-Seob;Jung, Jae-Hu;Kim, Dong-Soo
    • Korean Journal of Applied Biomechanics
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    • v.25 no.2
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    • pp.191-198
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    • 2015
  • Objective : The purpose of this study was to conduct biomechanical analysis of varying backpack loads on the lower limb movements during downhill walking over $-20^{\circ}$ ramp. Method : Thirteen male university students (age: $23.5{\pm}2.1yrs$, height: $175.7{\pm}4.6cm$, weight: $651.9{\pm}55.5N$) who have no musculoskeletal disorder were recruited as the subjects. Each subject walked over $20^{\circ}$ ramp with four different backpack weights (0%, 10%, 20% and 30% of body weight) in random order at a speed of $1.0{\pm}0.1m/s$. Five digital camcorders and two force plates were used to obtain 3-d data and kinetics of the lower extremity. For each trial being analyzed, five critical instants were identified from the video recordings. Ground reaction force, loading rate, decay rate, and resultant joint moment of the ankle and the knee were determined by the inverse dynamics analysis. For each dependent variable, one-way ANOVA with repeated measures was used to determine whether there were significant differences among four different backpack weight conditions (p<.05). When a significant difference was found, post hoc analyses were performed using the contrast procedure. Results : The results of this study showed that the medio-lateral GRFs at RHC in 20% and 30% body weight were significantly greater than the corresponding value in 0% of body weight. A consistent increase in the vertical GRFs as backpack loads increased was observed. The valgus joint movement of the knee at RTO in 30% body weight was significantly greater than the corresponding values in 0% and 10% body weight. The increased valgus moment of 30% body weight observed in this phase was associated with decelerating and stabilizing effects on the knee joint. The results also showed that the extension and valgus joint moments of the knee were systematically affected by the backpack load during downhill walking. Conclusion : Since downhill walking while carrying heavy external loads in a backpack may lead to excessive knee joint moment, damage can occur to the joint structures such as joint capsule and ligaments. Therefore, excessive repetitions of downhill walking should be avoided if the lower extremity is subjected to abnormally high levels of load over an extended period of time.

Classification of Polygonatum spp. Collections Based on Multivariate Analysis (다변량 분석에 의한 둥굴레속 식물의 분류)

  • Yun, Jong-Sun;Son, Suk-Yeong;Kim, Ik-Hwan;Hong, Eui-Yon;Yun, Tae;Lee, Cheol-Hee;Jong, Seung-Keun;Park, Sang-Il
    • Korean Journal of Medicinal Crop Science
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    • v.10 no.5
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    • pp.333-339
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    • 2002
  • This study was conducted to obtain the basic data for practical use of the Polygonatum genetic resources. The 20 collections were analyzed by principal component analysis of 8 characters and cluster analysis. In the principal analysis, the first, the second and the third components contributed 54.10%, 18.95% and 11.62% of the variations, respectively. The cumulative contribution from the first to the third principal components was 84.68%. The first principal component was related to shape and size of plant, and assimilatory, reserve and reproductive organs. The second principal component was related to growth and development of plant, and reserve organ. And the third principal component was related to growth and development of plant. Based on cluster analysis, the 20 collections were classified into 4 distinct groups with the average distance greater than 0.7 between groups. Group I was Polygonatum sibiricum $D_{ELAR}$ and Group II included P. odoratum var. pluriflorum $O_{HWI}$, P. odoratum var. pluriflorum $O_{HWI}$ for 'Variegatum' Y. Lee, for. nov., P. odoratum var. thunbergii $H_{ARA}$ and P. odoratum var. maximowiczii $K_{OIDZ}$. GroupIII was P. involucratum $M_{AXIM}$, P. desoulavyi $K_{OMAROV}$ and P. humile $F_{ISHER}$ ex. $M_{AXIM}$. And GroupIV included P. lasianthum var. coreanum $N_{AKAI}$ and P. inflatum $K_{OMAROV}$.

Effects of hydrogen peroxide pretreatment and heat activation of silane on the shear bond strength of fiber-reinforced composite posts to resin cement

  • Pyun, Jung-Hoon;Shin, Tae-Bong;Lee, Joo-Hee;Ahn, Kang-Min;Kim, Tae-Hyung;Cha, Hyun-Suk
    • The Journal of Advanced Prosthodontics
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    • v.8 no.2
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    • pp.94-100
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    • 2016
  • PURPOSE. To evaluate the effects of hydrogen peroxide pretreatment and heat activation of silane on the shear bond strength of fiber-reinforced composite posts to resin cement. MATERIALS AND METHODS. The specimens were prepared to evaluate the bond strength of epoxy resin-based fiber posts (D.T. Light-Post) to dual-curing resin cement (RelyX U200). The specimens were divided into four groups (n=18) according to different surface treatments: group 1, no treatment; group 2, silanization; group 3, silanization after hydrogen peroxide etching; group 4, silanization with warm drying at $80^{\circ}C$ after hydrogen peroxide etching. After storage of the specimens in distilled water at $37^{\circ}C$ for 24 hours, the shear bond strength (in MPa) between the fiber post and resin cement was measured using a universal testing machine. The fractured surface of the fiber post was examined using scanning electron microscopy. Data were analyzed using one-way ANOVA and post-hoc analysis with Tukey's HSD test (${\alpha}=0.05$). RESULTS. Silanization of the fiber post (Group 2) significantly increased the bond strength in comparison with the non treated control (Group 1) (P<.05). Heat drying after silanization also significantly increased the bond strength (Group 3 and 4) (P<.05). However, no effect was determined for hydrogen peroxide etching before applying silane agent (Group 2 and 3) (P>.05). CONCLUSION. Fiber post silanization and subsequent heat treatment ($80^{\circ}C$) with warm air blower can be beneficial in clinical post cementation. However, hydrogen peroxide etching prior to silanization was not effective in this study.

Effects of Dietary Chromium Methionine on Growth Performance, Carcass Composition, Meat Colour and Expression of the Colour-related Gene Myoglobin of Growing-finishing Pigs

  • Li, Y.S.;Zhu, N.H.;Niu, P.P.;Shi, F.X.;Hughes, C.L.;Tian, G.X.;Huang, R.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.7
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    • pp.1021-1029
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    • 2013
  • To investigate the effect of dietary chromium (Cr) as Cr methionine (CrMet) on growth performance, carcass traits, pork quality, meat colour and expression of meat colour-related genes in growing-finishing pigs, 189 crossbred Duroc${\times}$(Landrace${\times}$Yorkshire) growing-finishing pigs (male, castrated, average initial BW $74.58{\pm}1.52$ kg) were selected and randomly allocated into four groups. Dietary treatments per kg of feed were as follows: 0 (CT), 0.3 mg/kg (T1), 0.6 mg/kg (T2) and 0.9 mg/kg (T3) Cr (in the form of CrMet; as-fed basis), and each treatment was replicated five times with 8 to 10 pigs per replicate pen. During the 28 d of the experiment, both the ADG and the ADFI increased linearly (p<0.05) as the level of dietary Cr increased. The F/G ratio decreased linearly (p<0.05). As dietary Cr increased, loin muscle areas (linear, p = 0.013) and average backfat thickness (linear, p = 0.072) decreased. Shear force (linear, p = 0.070) and Commission Internationale de I'$\acute{E}$clairage (CIE) redness (quadratic, p = 0.028) were increased. In addition, CIE Lightness (quadratic, p = 0.053) were decreased as dietary Cr increased. As dietary Cr increased, total myglobin (Mb) content (quadratic, p = 0.015) and the mb mRNA levels (quadratic, p = 0.046) in longissimus muscles of pigs were up-regulated. In conclusion, supplementation of dietary Cr improved growth and meat colour, but increased shear force and decreased IMF reduced palatability of longissimus muscles. Moreover, the increasing total Mb content and mb mRNA levels indicated that CrMet dietary supplementation may improve meat colour via up-regulating expression of the mb gene.

Minimum Cost Path for Private Network Design (개인통신망 설계를 위한 최소 비용 경로)

  • Choe, Hong-Sik;Lee, Ju-Yeong
    • Journal of KIISE:Computer Systems and Theory
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    • v.26 no.11
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    • pp.1373-1381
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    • 1999
  • 이 논문에서는 통신망 설계 응용분야의 문제를 그래프 이론 문제로써 고려해 보았다. 개별 기업체가 서로 떨어진 두 곳을 연결하고자 할 때 공용통신망의 회선을 빌려 통신망을 구축하게 되는데 많은 경우 여러 종류의 회선들이 공급됨으로 어떤 회선을 선택하느냐의 문제가 생긴다. 일반적으로 빠른 회선(low delay)은 느린 회선(high delay)에 비해 비싸다. 그러나 서비스의 질(Quality of Service)이라는 요구사항이 종종 종단지연(end-to-end delay)시간에 의해 결정되므로, 무조건 낮은 가격의 회선만을 사용할 수는 없다. 결국 개별 기업체의 통신망을 위한 통로를 공용 통신망 위에 덮어씌워(overlaying) 구축하는 것의 여부는 두 개의 상반된 인자인 가격과 속도의 조절에 달려 있다. 따라서 일반적인 최소경로 찾기의 변형이라 할 수 있는 다음의 문제가 본 논문의 관심사이다. 두 개의 지점을 연결하는데 종단지연시간의 한계를 만족하면서 최소경비를 갖는 경로에 대한 해결을 위하여, 그래프 채색(coloring) 문제와 최단경로문제를 함께 포함하는 그래프 이론의 문제로 정형화시켜 살펴본다. 배낭문제로의 변환을 통해 이 문제는 {{{{NP-complete임을 증명하였고 {{{{O($\mid$E$\mid$D_0 )시간에 최적값을 주는 의사선형 알고리즘과O($\mid$E$\mid$)시간의 근사 알고리즘을 보였다. 특별한 경우에 대한 {{{{O($\mid$V$\mid$ + $\mid$E$\mid$)시간과 {{{{O($\mid$E$\mid$^2 + $\mid$E$\mid$$\mid$V$\mid$log$\mid$V$\mid$)시간 알고리즘을 보였으며 배낭 문제의 해결책과 유사한 그리디 휴리스틱(greedy heuristic) 알고리즘이 그물 구조(mesh) 그래프 상에서 좋은 결과를 보여주고 있음을 실험을 통해 확인해 보았다.Abstract This paper considers a graph-theoretic problem motivated by a telecommunication network optimization. When a private organization wishes to connect two sites by leasing physical lines from a public telecommunications network, it is often the cases that several categories of lines are available, at different costs. Typically a faster (low delay) lines costs more than a slower (high delay) line. However, low cost lines cannot be used exclusively because the Quality of Service (QoS) requirements often impose a bound on the end-to-end delay. Therefore, overlaying a path on the public network involves two diametrically opposing factors: cost and delay. The following variation of the standard shortest path problem is thus of interest: the shortest route between the two sites that meets a given bound on the end-to-end delay. For this problem we formulate a graph-theoretical problem that has both a shortest path component as well as coloring component. Interestingly, the problem could be formulated as a knapsack problem. We have shown that the general problem is NP-complete. The optimal polynomial-time algorithms for some special cases and one heuristic algorithm for the general problem are described.

Diffusible and Volatile Antifungal Compounds Produced by an Antagonistic Bacillus velezensis G341 against Various Phytopathogenic Fungi

  • Lim, Seong Mi;Yoon, Mi-Young;Choi, Gyung Ja;Choi, Yong Ho;Jang, Kyoung Soo;Shin, Teak Soo;Park, Hae Woong;Yu, Nan Hee;Kim, Young Ho;Kim, Jin-Cheol
    • The Plant Pathology Journal
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    • v.33 no.5
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    • pp.488-498
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    • 2017
  • The aim of this study was to identify volatile and agardiffusible antifungal metabolites produced by Bacillus sp. G341 with strong antifungal activity against various phytopathogenic fungi. Strain G341 isolated from four-year-old roots of Korean ginseng with rot symptoms was identified as Bacillus velezensis based on 16S rDNA and gyrA sequences. Strain G341 inhibited mycelial growth of all phytopathogenic fungi tested. In vivo experiment results revealed that n-butanol extract of fermentation broth effectively controlled the development of rice sheath blight, tomato gray mold, tomato late blight, wheat leaf rust, barley powdery mildew, and red pepper anthracnose. Two antifungal compounds were isolated from strain G341 and identified as bacillomycin L and fengycin A by MS/MS analysis. Moreover, volatile compounds emitted from strain G341 were found to be able to inhibit mycelial growth of various phytopathogenic fungi. Based on volatile compound profiles of strain G341 obtained through headspace collection and analysis on GC-MS, dimethylsulfoxide, 1-butanol, and 3-hydroxy-2-butanone (acetoin) were identified. Taken together, these results suggest that B. valezensis G341 can be used as a biocontrol agent for various plant diseases caused by phytopathogenic fungi.

Determination of Adequate Method for Protein Extraction from Rice Bran and the Substitution of Dried Skim Milk with Protein Concentrate from Rice Bran in Early Weaned Pigs

  • Phipek, W.;Nagasinha, C.;Vallisuth, S.;Nongyao, C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.9
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    • pp.1268-1273
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    • 2011
  • The present study was conducted to determine a feasible method of protein concentrate extraction from rice bran (RBPC) and its effect as a substitution for skim milk in early weaning pig diets. An investigation to extract protein concentrate from full fat rice bran was undertaken to determine the best ratio of water and rice bran, the amount of NaOH and a HCl solvent to use in a simple paddle-type mixer with modified spinning to produce RBPC. The results stated that the best ratio for water mixing in the RBPC extraction process was 1:5 with 20 g NaOH and 30 min in a paddle-type mixer at 300 rpm. A mix of 250 ml 0.2 N HCl was optimum for neutralization and protein precipitation. After the fluid was spun out with a washing machine, the sediment was left for 12-14 hours to complete the filtration. One kilogram of rice bran could produce an average of 324.5 gram RBPC and it contained 3.40% ash, 496.48 kcal of GE/100 gram, 1.94% crude fiber, 28.20% ether extract, 7.64% moisture and 16.66% crude protein, respectively. A total of 45 crossbred piglets, weaned at 3 weeks of age were allotted into control diet (A) and dietary treatments formulated with a four different rates of RBPC substitution for skim milk at a percentage of 25 (B), 50 (C), 77 (D) and 100 (E) respectively, in a randomized complete block (RCB) design. All piglets had free access to feed and water until 8 week of age when the experiment ended. Feed intake, average daily gain, growth rate and feed efficiency were not affected by dietary treatments. Blood test parameters after completion of the growth trial indicated normal health. Even though the mean of cell hemoglobin concentration was significantly different between treatments (p<0.05) it was still within the normal range. The cost difference for BW gain of 100% RBPC substituted for skim milk in the weaning diet was approximately 35% lower than that of the control and the relative cost of production was 96.67, 92.85, 70.75 and 64.48% lower for the replacement of 25, 50, 75 and 100% of skim milk respectively. These results implied that this technology is feasible for use by small scale farmers to improve their self-reliance.