• The Journal of Internal Korean Medicine
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• v.15 no.2
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• pp.37-47
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• 1994

To investigate the effect of ILGUAN-JEON on liver and gastrointestinal track. this experiment were performed and obtained results wewe as follows. 1. ILGUAN-JEON depressed the isolated intestine movement and showed anti- acethylcholine and barium chloride effect in mice. 2. ILGUAN-JEON showed anti- acethylcholine and barium chloride effect on isolated stomach in rats. 3. ILGUAN-JEON showed anti-ulcer effect in rats induced by indomethacin and pyrous cracking. 4. ILGUAN-JEON depressed the gastric lesion in rats induced by HCI-ethanol. 5. ILGUAN-JEON depressed the gastric juice and pepsin secretion, free acid and total acid level in Shay rats. 6. ILGUAN-JEON depressed the GOT and GPT level in rats induced by $CCl_4$ and d-galactosamine. From above results, think that ILGUAN-JEON can be applied to recover the liver and gastrointestinal function.

• Proceedings of the PSK Conference
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• 2002.04a
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• pp.221.2-222
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• 2002

• Applied Microscopy
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• v.23 no.2
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• pp.84-96
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• 1993

This study was to investigate the effects of maternal diabetes on the lung tissue of the fetal rat using lectin histochemistry and electron microscope technique. Maternal diabetes was induced by intraperitoneal injection of streptozotocin (75 mg/kg the body weight) into pregnant Sprague-Dawley rats on the 7th day of gestation. Fetuses of streptozotocin induced diabetic rats exhibited delayed lung maturation and reduced air space. In lectin histochemistry, the binding of Maclura pomifera (MPA) to fetal lungs from diabetic mothers was reduced, but no significant changes in the bindings of Concanavalin A (Con A), Wheat germ agglutinin (WGA), Ricinus communis I (RCA I) and Griffonia simplicifolia (GSI-$B_4$) were noted. Because the MPA has affinity to terminal N-acetyl-D-galactosamine residues constantly linked O-glycosidically to serine or threonine, the present findings may indicates that maternal diabetes interfere with the processes of O-linked glycosylation in fetal rat lung.

• Biomolecules & Therapeutics
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• v.6 no.2
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• pp.119-129
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• 1998

The dose-response effect of polysaccharide extracts(PS-1) from Artemisia iwayomogi was inves-tigated under various hepatic disease models. Silymarin, DDB and UDCA were used as reference compounds. We found that the maximal effective dose of PS-1 was 100 mg/kg b.wt. in $CCl_4$-induced hepatotoxicity, D-galactosamine-induced hepatitis, in ANIT-induced cholestasis and 300 mg/kg b.wt. in $CCl_4$-induced chronic liver disease, 30 mg/tg b.wt. in chronic bile duct ligation and chronic ethanol fatty liver. These findings suggest that PS-1 fraction protects the hepatocyte against various hepatic injuries, and this fracton might be of therapeutic value.

• YAKHAK HOEJI
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• v.28 no.1
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• pp.35-48
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• 1984

우리나라 사람들에게는 유달리 높은 간염 발병률이 나타나고 있고 더우기 바이러스성 간염의 경우 풍토병 내지 전염병이라고 할 정도의 양상을 띠고 있다. 현재까지는 이러한 간질환에 적절한 치료약이 없는 점을 감안하여 저자들은 생약으로 부터 간질환에 유효한 성분을 분리하여 치료약으로 사용할 수 있도록 하기위하여 집중적인 연구를 지난 5년간 계속하여 왔다. 연구의 내용은 동서양의 고전및 연구보문을 조사하여 간보호 및 치료학으로 사용한 생약에 관한 문헌적 조사, 이들 생약중 채집 및 구입이 가능한 식물을 구하여 생약엑기스 제조, 이들 엑기스를 간염 동물 모델의 하나인 $CCl_{4}$로 유발시킨 간독성에 대한 보호작용의 검색, 보호작용을 나타내는 생약중에서 자원적 측면을 고려하여 국내에서 많이 생산되는 차전자(Plantago asiatica seeds)를 선택하였고, 차전자로 부터 간보호 작용을 나타내는 유효성분으로 iridoid 계열물질인 aucubin을 분리하였다. 그러나 aucubin이 차전자에 소량 밖에 없으므로 aucubin이 다량 함유된 식나무(Aucuba japonica)로 aucubin 추출자료생약을 바꾼후 aucubin의 간보호 작용을 간염 동물모델인, $CCl_{4}$ D-galactosamine 및 $\alpha$-amanitin등으로 유발시킨 간독성에 대한 보호작용을 보여 주었기 때문에, 이러한 간보호작용의 기전을 규명하는 연구를 진행하였고, 다음은 aucubin 자체의 급성 독성 및 기타 독성 유발여부를 밝히는 연구 등으로 요약 될 수 있다.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.204.2-204.2
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• 2003

The aqueous extract of European mistletoe (Viscum album, L.) has been used in cancer therapy. The purified mistletoe lectins, main components of mistletoe, have demonstrated cytotoxic and immune-system-stimulating activities. Korean mistletoe (Viscum album L. coloratum), a subspecies of European mistletoe, has also been reported to possess anticancer and immunological activities. A galactose- and N-acetyl-D-galactosamine- specific lectin (Viscum album L. coloratum agglutinin, VCA) with Mr 60 kDa was isolated from Korean mistletoe. (omitted)

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.251.2-251.2
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• 2002

2-Methylaminoethyl- 4,4' -dimethoxy- 5, 5',6,6' -dimethylenedioxybiphenyl- 2' -carboxy- 2-carboxylate (DDB-S) is a synthetic compound derived from DDB. which is protects liver against carbon tetrachloride-, D-galactosamine-, thioacetamine-, and prednisolone- induced hepatic injury in experimental animals. We assessed the use of liquid chromatography/electrospray iontrap tandem mass spectrometry (LC/MS/MS) method to identify and quantify in vivo metabolites and to measure excretion. (omitted)

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.8
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• pp.1286-1293
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• 2004

This study was conducted to investigate the biological activity and hepatoprotective effect of various fractions and isolated compounds from Kochiae fructus (KF) extract on D-galactosamine (GaIN)-intoxicated rats. Male Sprague-Dawley rats were divided into control, GaIN treated group (GaIN), GaIN plus KF methanol extract treated group (KFM 200-GaIN), GaIN plus KF butanol extract treated group (KFB 200-GaIN), GaIN plus momordin Ic treated group (Momordin Ic 30-GaIN) and GaIN plus oleanolic acid treated group (Oleanolic acid 30-GaIN). KFM (200 mg/kg BW), KFB (200 mg/kg BW), momordin Ic (30 mg/kg BW) and oleanolic acid (30 mg/kg BW) were orally administered once a day for 14 days. GaIN (400 mg/kg BW) was injected at 30 minutes after the final administration of the compounds. The activities of serum aspartate aminotransferase and alanine aminotransferase were increased in the GaIN group compared to the control group and significantly lower in the KFB 200-GaIN, momordin Ic 30-GaIN and oleanolic acid 30-GaIN group than in the GaIN group. Hepatic lipid peroxide level was increased in the GaIN group compared to the control group and was lower in the KFM 200-GaIN, KFB 200-GaIN, momordin Ic 30-GaIN and oleanolic acid 30-GaIN group than in the GaIN group. Activities of xanthine oxidase and aldehyde oxidase in liver were higher in the GaIN group than in the control group and were significantly decreased in the KFB 200-GaIN, momordin Ic 30-GaIN and oleanolic acid 30-GaIN group compared to the GaIN group. Hepatic glutathione, ${\gamma}$-glutamylcysteine synthetase and catalase activities were decreased in the GaIN group compared to the control group and were higher in the KFB 200-GaIN, momordin Ic 30-GaIN and oleanolic acid 30-GaIN group than in the GaIN group. Activities of hepatic glutathione reductase, glutathione S-transferase, superoxide dismutase and glutathione peroxidase were lower in the GaIN group than in the control group and were improved in the KFM 200-GaIN, KFB 200-GaIN, momordin Ic 30-GaIN and oleanolic acid 30-GaIN group compared to the GaIN group. Therefore, the current results indicate that momordin Ic administration alleviated the GaIN-induced adverse effect through enhancing the antioxidant enzyme activities.

• The Korean Journal of Food And Nutrition
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• v.32 no.2
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• pp.106-113
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• 2019

The objective of this study was to evaluate the effect of fermented Kalopanax pictus (KP-F) on macrophage activation and its effect as a competitive inhibitor of LPS and inhibitory effect on endotoxemia. The results showed that KP-F could activate macrophage in a dose-dependent manner, and KP-F was confirmed to act as a ligand for TLR4. Also, it was found that KP-F did not exhibit the same biotoxicity as LPS in intraperitoneal injection, and that it could suppress the neutrophil migration induced by LPS administration. In normal mice, the body weight, tissue weight, and amount of nitrite and pro-inflammatory cytokines in serum showed no significant changes with KP-F diet for 2 weeks, confirming that administration of KP-F in normal mice did not lead to over activation of immune response and biotoxicity. In the mouse model of endotoxemia induced by LPS and D-galactosamine(D-GalN) in sub-lethal dose, the diet of KP-F effectively inhibited the amount of nitrite and cytokines in the blood, and thus was found to be able to relieve the hepatic and kidney injury. In addition, in the endotoxemia mouse model induced by LPS and D-GalN of lethal dose, the survival rate was increased by KP-F diet in a dose-dependent manner.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.626-629
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• 2003

The water extracts of Hovenia dulcis Thunb. showed significant reducing alcohol concentration in blood and hepatoprotective acitivity against D-galactoseamine/LPS and CCl4-toxicity. The concentration of blood alcohol was decreased from 2hr after injected in rats with Hovenia dulcis extracts. The water extracts of Hovenia dulcis significantly inhibited the elevation of serum ALT, AST and LDH levels. In this study, we investigated the effect of Hovenia dulcis extract on reducing alcohol concentration in blood. Also the efficacy and toxicity of Hovenia dulcis extract were evaluated by in vivo and in vitro tests.