• 한국환경보건학회지
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• 제41권1호
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• pp.17-23
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• 2015

Objectives: The cytotoxcities of Au, Ag, SWCNT, $SiO_2$, and ZnO nanomaterials were evaluated in order to assess their potential toxicological effects in in vitro cell models using colony forming efficiency (CFE) assay. Methods: The CFE assay of the test materials was carried out on Hep G2 cells. The size distribution of nanomaterials was studied by transmission electron microscopy (TEM). Changes in cell viability after treatment with a toxicant will result in a decreased number of colonies formed in comparison to solvent. Results: The TEM images show that all the particles except SWCNT and ZnO can be considered approximately spherical. The gold and $SiO_2$ nanoparticles show no response (no toxicity) in concentration response experiments. A statistically significant toxic effect was found in Hep G2 cells treated with Ag, SWCNT and ZnO nanomaterials. Conclusion: In this study, we considered CFE assay to be a promising test for screening studies for cytotoxicity with physicochemical analysis.

• 생명과학회지
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• 제19권7호
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• pp.963-967
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• 2009

본 연구에서는 glutamate로 유도된 세포독성으로부터 신경세포를 보호하는 상백피(Morus alba) 추출물의 활성을 확인하기 위하여 N18-RE-105 세포주를 이용하여 MTT reduction assay, LDH release assay 및 광학 현미경을 이용하여 형태학적인 변화를 관찰하였다. 그 결과, 상백피 methanol 추출물에서 농도 의존적으로 신경세포 보호효과가 나타났으며, 50 본 연구에서는 glutamate 로 유도된 세포독성으로부터 신경세포를 보호하는 상백피 (Morus alba) 추출물의 활성을 확인하기 위하여 NI8-RE-I05 세포주를 이용하여 MTT reduction assay, LDH release assay 및 광학 현 미 경 을 이 용하여 형태학적인 변화를 관찰하였다. 그 결과y 상백피 methanol 추출물에서 농도 의존적으로 신경세포 보호효과가 나타났으며, 50 ${\mu}g$/ml 농도에서는 80% 이상의 세포생존율을 확인할수 있었다. 이 결과는 N18-RE-105 세포주의 LDH release assay와 형태학적 변화에서도 일치하는 결과를 확인하였다. 가장 높은 활성을 보인 상백피 methanol 추출물을 hexane, diethyl ether, ethyl acetate, water 층으로 분획하여 각 각 1, 5, 10 ${\mu}g$/ml 농도로 처리 시 hexane 층에서 48.0%, 65.6%, 71.5%로 가장 높은 신경세포 보호효과를 확인할 수 있었다. 따라서 상백피 추출물은 glutamate 에 의한 세포독성으로부터 신경세포 손상을 억제하며 신경세포를 보호하는 효과가 있다는 것을 알 수 있었다.

• 한국식품조리과학회지
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• 제28권2호
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• pp.175-182
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• 2012

In this study, antibacterial activity and cytotoxicity of the extracts from pericarp and seed of $Vitis$ $coignetiea$, which were extracted with 0.1% HCl-60% ethanol, were analyzed. The antibacterial activity of the extracts was determined by paper disc diffusion method against food spoilage and food-borne pathogens. The pericarp extract showed high antibacterial activity against $Bacillus$ $cereus$, $Escherichia$ $coli$ O157:H7, and $Pseudomonas$ $aeruginosa$, and the seed extract represented the high antibacterial activity against $B.$ $cereus$, $E.$ $coli$ O157:H7, and $Staphylococcus$ $aureus$. The cytotoxicity of the $Vitis$ $coignetiea$ extract against human cancer cells was determined using the MTT assay and SRB assay. The pericarp extract represented strong growth-inhibition activity against G361 and Hep3B cells and the seed extract greatly inhibited the growth of HeLa and G361 cells in the MTT assay. In addition, the pericarp extract displayed a high inhibition activity against the growth of AGS cells and the seed extract greatly inhibited the growth of HeLa, Hep3B, and MCF7 cells in the SRB assay. Especially, the cytotoxicities of the seed extract against HeLa were significantly higher than those of the extract against other cancer cells at all test concentrations. This study demonstrates that the extract from pericarp and seed of $Vitis$ $coignetiea$ possess high antibacterial activity and cytotoxicity.

• 한방안이비인후피부과학회지
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• 제20권1호통권32호
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• pp.145-153
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• 2007

Objecgtive : The aim of present study was to investigate inhibition effect of Whakijogyung-Tang(WJT) on the tumor cell lines. This study estimated the cytotoxicity of WJT about viability of S-180 and NlH3T3. Methods : The cytotoxicity of WJT about viability of cells were tested using a colorimetric tetrazoliun assay(MTT assay) Results and Conclusion : 1. Water extract of WJT had $IC_{50}$ of 863 ${\mu}g/ml$ in S-180 cell lines, but cytotoxicity of NIH3T3 was not significant difference compare with S-180. 2. n-Hexane fraction of WJT had similar cytotoxicity between S-180 and NIH3T3, but that could not have $IC_{50}$ in S-180 cell lines. 3. Ethyl acetate fraction of WJT had low degree cytotoxicity both S-180 and NIH3T3 cell lines. 4. Significantly, Butanol fraction of WJT had differenct citotoxicity between S-180 and NIH3T3. 5. $H_2O_2$ fraction of WJT had no cytotoxicity both S-180 and NIH3T3.

• 한방안이비인후피부과학회지
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• 제20권1호통권32호
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• pp.169-176
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• 2007

Objectives : The purpose of this study was to investigate inhibitory effect of Gagamtongsung-San(GTS) on the cancer. Methods : This study estimated the cytotoxicity of GTS about L1210 and NIH3T3. We used GTS extract distilled with water, n-Hexane, Ethyl acetate and Butanol. The cytotoxicitys of GTS about cancer cells and normal cells were tested using a colorimetric tetrazoliun assay(MTT assay). Results : The results of this study were obtained as follow ; l. Cytotoxicity of water extract of GTS in L1210 cell lines was significantly increased, compared with NIH3T3. 2. n-Hexane fraction of GTS had similar cytotoxicity between L1210 and NIH3T3, and that have similar $IC_{50}$ of water extract of GTS at 276 ${\mu}g/ml$ 3. Ethyl acetate fraction of GTS had low degree cytotoxicity both L1210 and NIH3T3 cell lines. 4. Butanol fraction of GTS had cytotoxicity between L1210 and NIH3T3. Significantly, Cytotoxicity of GTS in L1210 cell lines was significant increased. 5. $H_2O$ fraction of GTS had no cytotoxicity both L1210 and NIH3T3.

• 대한치과보철학회지
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• 제40권4호
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• pp.309-322
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• 2002

The purpose of this study was to investigate the cytotoxicity and mutagenicity of denture base resins. According to manufacturer's instructions, resin specimens were made. Group 1 : heat-polymerizing acrylic resin (Luciton $199^{(R)}$) Group 2 : heat-polymerizing acrylic resin containing polyhedraloligosilsesquioxane(POSS resin) Group 3 : auto-polymerizing acrylic resin (Repair $Acrylic^{(R)}$) Group 4 : direct relining auto-polymerizing acrylic resin (Tokuso $Rebase^{(R)}$). Fresh specimens 24 hrs. and 72 hrs. soaked specimens in distil)ed water were made. Responses with metabolic assay and mutagenesis assay to eluates from resin specimens were measured. Cultures with medium alone provided controls. Cytotoxicity was assessed with agar overlay test. The results were as follows; 1. Group 4 showed higher cytotoxicity than Group 1, Group 2 and Group 3 in fresh, 24-an4 72-hour immersion caries (p<.05). Group 3 showed higher cytotoxicity than Group 2 in fresh cases and showed higher cytotoxicity than Group 1 and Group 2 in 24-and 72-hour immersion cases (p<.05) . Group 1 and Group 2 showed no significant difference. 2. All acrylic denture base resins skewed significant increase of cell activity as immersion time increased (p<.05). 3. Auto-polymerizing acrylic denture base resins skewed higher cytotoxicity than heat-polymerizing acrylic denture base resins (p<.05). 4. All acrylic denture base resins showed lower mutagenicity than controls (p<.05).

• Toxicological Research
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• 제14권3호
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• pp.315-320
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• 1998

In vitro skin iritation of anti-inflammatory drugs was investigated in terms of the cytotoxicity method to human skin fibroblast cells. Five anti-inflammatory drugs (Diclofenac, Naproxen, Meclofenamic acid, Ibuprofen and Fnoprofen) which are commercially available as oral preparations or injections were tested. The cytotoxicity of 5 chemicals was evaluated by using MTT[tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. NRU (neutral red uptake) assay and Alamar Blue assay after fibroblast cells had been exposed to the chemicals for 24 hours or 489 hours. The $IC_{50}$ values of the chemicals showed the comparative strength of cytotoxicity as following order of Meclofenamic acid>Diclofenac>Fenoprofen>Ibuprofen>Naproxen. The values of $IC_{50}$ determined by Alamar Blue assay were lower than those of MTT and NRU assay. These data suggest Alamar Blue assay can be useful method for assessing in vitro skin irritation potential of anti-inflammatory drugs.

• 대한치과교정학회지
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• 제34권4호
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• pp.351-362
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• 2004

고정식 교정 치료시 교정용 밴드가 장착된 치아의 치주조직 손상은 접착된 치아에서 보다 자주 발생한다. 일반 치과치료에 사용되는 시멘트는 세포독성을 지닌 것으로 알려져 있으므로 치주조직과 직접 접촉하거나 구강 내에서 밴드시멘트의 성분들이 용출되는 교정용 밴드 시멘트는 치주조직의 손상을 일으키는 원인이 될 수 있다 그러므로 교정용 밴드 시멘트의 생체 친화성은 재료의 물성과 함께 중요하게 고려되어야 할 사항이다. 이 연구의 목적은 교정용 밴드 시멘트의 세포독성을 평가하는 것으로 현재 임상에서 널리 사용되고 있는zinc phosphate cement (ZPC), glass ionomer (GI), resin modified glass ionomer (RMGI), compomer의 생체 친화성을 평가하기 위해 human gingiva fibroblast를 배양하고 이 세포에 대한 세포수 산정 및 세포 형태관찰, MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, 한천중층시험 등의 세포독성 검사를 시행하여 다음과 같은 결과를 얻었다. 1. 세포수 산정 및 형태 관찰 시험에서 Gl>ZPC, RMGI, RMG124, GI24>compomer24, ZPC24, compomer 순으로 세포독성이 높게 나타났다. 2. MTT assay 에서는 GI>ZPC, RMGI>GI24>ZPC24, compomer, RMGI24, compomer24 순으로 세포독성이 높게 나타났다. 3. 한천중층시험에서 는 GI>GI24, ZPC, ZPC24, RMGI>RMG124, compomer, compomer24 순으로 세포독성 이 높게 나타났다.

• Journal of Nutrition and Health
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• 제35권4호
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• pp.439-445
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• 2002

본 연구에서는 예로부터 민간과 한방에서 널리 이용되어 온 오매를 이용하여 오매 추출물이 간암 및 자궁암 세포주 증식에 미치는 효과를 알아보았다. 오매를 hexane. chloroform, ethylacetate, methanol 및 열수로 순차 추출하여 분획물을 농도별로 조제하였으며, MTT assay, cytotoxicity test 및 현미경 관찰 등을 통하여 암세포의 성장 억제 효과를 알아보았다. Hep G2의 경우 오매의 ethylacetate추출물이 가장 큰 성장 저해 효과를 나타내었으며 MTT assay결과 250 $\mu\textrm{g}$/ml 농도 이상에서부터 유의적인 증식 억제 효과를 보였다. 또한 cytotoxicity test결과 오매의 ethylacetate 추출물 1000$\mu\textrm{g}$/ml농도에서 72시간 배양시 75%의 세포 파괴율을 나타내었으며 현미경 관찰 결과 세포의 모양이 변화되고 죽은 세포가 배양액위에 떠있음을 관찰할 수 있었다. HeLa cell에서는 오매의 hexane 추출물이 가장 큰 저해 효과를 나타내었으며 MTT assay 결과 250 $\mu\textrm{g}$/ml 농도 이상에서부터 유의적인 증식 억제 효과를 보였다 또한 cytotoxicity test 결과 오매의 hexane추출물 1000 $\mu\textrm{g}$/ml농도에서 72시간 배양시 HeLa cell의 68%가 파괴되었으며 현미경 관찰 결과 세포 모양의 형태학적 변화가 일어났다. 정상세포주인 fibroblast에는 오매의 ethylacetate 및 hexane 추출물이 아무런 유의적 영향을 미치지 않았으며 현미경 관찰 시에도 변화가 일어나지 않았다

• 한국미생물·생명공학회지
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• 제25권5호
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• pp.477-482
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• 1997

To investigate the cytotoxicity of Korean traditional fermented soybean products using the MTT assay, we extracted soybean, Kanjang, Doenjang, Kochujang, and Chongkukjang with water, methanol, and hexane. Primary testing of cytotoxicity of 14 extracts was done for P388D1(mouse lympoid neoplasm) and L1210(mouse leukemia) cell lines. Doenjang methanol extract, Kochujang hexane extract, Chongkukjang methanol extract, and Chongkukjang hexane extract showed cytotoxicity of 86.1, 94.3, 83. 6, and 81.1%, respectively against P388D1, and showed cytotoxicity of 69.4, 96.9, 51.4, and 95.1%, respectively against L1210. All the other extracts showed less than 50% cytotoxicity. Methanol extracts of Doenjang and Chongkukjang showed dose-dependent cytotoxicity against P388DI, L1210, SNU-16 (human stomach cancer), HepG2(human hepatic cancer), WiDr(human colon cancer) cell lines, and IC$_{50}$ of Doenjang methanol extract was 67.7, 90.4, 1338.0, 706.4, and 371.2 $\mu$g/ml, respectively, and IC$_{50}$ of Chongkukjang methanol extract was 107.1, 228.3, 756.2, 1346.0, and 327.0 $\mu$g/ml, respectively.