• Korean Journal of Acupuncture
• /
• 제37권1호
• /
• pp.37-45
• /
• 2020

Objectives : Mitochondria are typically known as intracellular double membrane-bound structures that supply energy for intracellular metabolism including Krebs cycle and beta-oxidation. Also, acupuncture has been known to stimulate the flow of energy. To explore the effect of acupuncture on the mitochondrial respiratory chain activities in rat's heart and kidneys, the activities of mitochondrial respiratory chain complexes I to IV were observed. Methods : The rats were divided into 11 groups; Normal (no acupuncture treatment and under anesthesia for 10 min), heart meridian five-transport-points (acupuncture treatment at HT9, HT8, HT7, HT4 and HT3 under anesthesia for 10 min), and kidney meridian five-transport-points (acupuncture treatment at KI1, KI2, KI3, KI7 and KI10 under anesthesia for 10 min). All rats were sacrificed and the heart and kidneys were examined for the changes of respiratory chain activities. Results : Acupuncture at HT7 increased the activity of succinate dehydrogenase; acupuncture at KI2 increased the activity of ubiquinol cytochrome C oxidoreductase; and acupuncture at HT9, HT8, HT3 and KI1 increased activities of cytochrome C oxidase. Conclusions : Acupuncture assists mitochondrial repiratory chain activity via the Cytochrome C oxidase signaling pathway in heart and kidney of rats.

• BMB Reports
• /
• 제29권2호
• /
• pp.169-174
• /
• 1996

Yeast cytochrome c (cyt c) was modified at cysteine-102 with a thiol-specific spin label and its interaction with liposomes containing acidic phospholipids was studied by electron paramagnetic resonance (EPR) spectroscopy. Association of cyt c with liposomes resulted in a significant reduction in the mobility of the spin label and a fraction of cyt c even seemed to be immobilized. Based on a large spectral change upon binding and the proximity of the spin-label to lysine-86 and -87, we propose these two residues to be the potential binding site at neutral pH. The interaction is electrostatic in nature because the spectral changes were reversed by addition of anions. Dissociation of the bound cyt c by anions, however, became less effective as the lipid/protein ratio increased. This suggests a repulsive lateral interaction among the bound cyt c. Unlabeled cyt c molecules added to preformed cyt c-liposome complex displaced the bound (spin labeled) cyt c and the process was competitive and reversible.

• 한국식품영양과학회지
• /
• 제26권5호
• /
• pp.901-907
• /
• 1997

The purpose of this study was to investigate the effects of green tea catechin o n free radical generation system and peroxidative damage in the liver of streptozotocin(STZ)-induced diabetic rats. Spragu-Dawley male rats weighing 150$\pm$10gm were randomly assigned to one normal and three STZ-induced diabetic groups; diabetic groups were classified to catechin free diet(DM-oC group), 0.5% catechin diet(DM-0.5C group) and 1% catechin diet(DM-1C group) according to the levels of dietary catechin supplementation. Diabetes was experimentally induced by intravenous injection of 55mg/kg of body wt of STZ in citrate buffer(pH 4.3) after feeding of three experimental diet for 4 weeks. Animals were sacrificed at the 6th day of diabetic states. Activities of serum glutamic oxaloacetic transaminase(GPT) in DM-oC groups were higher than those of the normal group, and those in catechin supplementation group were similar to those of the normal group. Liver lipid peroxide values increased by 153%, 49%, and 27% in Dm-oC, DM-0.5C and DM-0C and Dm-1C but was not significantly different in catechin supplementation groups compared with the normal group, and liver cytochrome $P_{450}$ contents was similar to result of XOD activity. In electron microscopic examination of liver, lysosome was relatively scattered in Dm-oC and Dm-0.5C group and preserved normal shapes in DM-1C group. The present results indicate that STZ-induced diabetic rats are more sensitive to oxidative stress, leading to the acceleration of lipid peroxidation process, but this was reduced by anti-oxidative effect of high level of dietary catechin. It is concluded that dietary catechin serves as powerful antioxidant against lipid peroxidation in diabetic rats.

• 생명과학회지
• /
• 제8권6호
• /
• pp.695-701
• /
• 1998

한국산 꽃뱀(Natrix tigrina Lateralis)의 간 마이크로좀에 존재하는 mixed function oxidase system 구성 성분들의 함량과 P45O 의존성 monooxygenase의 활성도를 조사하고 이들을 흰쥐(Sp. D)의 것과 상호 비교하였다. 꽃뱀에서의 P45O, b5 함량 및 NADPH-cytochrome c reductase 활성도는 흰쥐에서 보다 낮았으며, 7-ethoxycoumarin O-deethylase와 benzphetamine N-demethylase 활성도 역시 흰쥐에서 보다 상당히 낮았다. 그러나 aryl hydrocarbon hydroxylase와 testosterone hydroxylase 활성도는 흰쥐와 비교할 때 거의 비슷하거나 오히려 높았다. Testosterone의 수산화 반응에 대한 선택특이성을 조사한 결과, 꽃 뱀은 7$\alpha$ 위치에서, 흰쥐는 6$\beta$ 위치에서 가장 높은 수산화 반응물을 생성했다. 그러나 testosterone의 C2와 C3 위치에서의 수산화 반응에 대한 선택특이성은 꽃뱀과 흰쥐에서 비슷하였다. Radioimmunoassay (RlA)를 실시하여 5종 (CYP2B, CYP1A1, CYP1A2, CYP3A 및 CYP2El)의 P45O 동위효소의 구성비를 비교한 결과, 꽃뱀에서는 CYP1A1/1A2가, 흰쥐에서는 CYP2El이 각각 비교적 많이 존재하였다. 부분정제한 P45O을 SDS-PAGE와 RIA로 분석한 결과, 꽃뱀의 간 마이크로좀에 존재하는 P45O중에는 흰쥐와는 다른 종류의 P45O 동위효소가 존재함을 시사하였다.

• Applied Biological Chemistry
• /
• 제44권3호
• /
• pp.153-161
• /
• 2001

Ecdysteroid 생합성에 관련된 식물 유전자를 분리할 목적으로 ecdysteroid 생성능이 확인된 쇠무릎(Achyranthes japonica Nakai)으로부터 RNA를 분리하고, ecdysteroid 생합성에 관여한다고 알려진 cytochrome P45O family 유전자 4개와 곤충의 ecdysone 20-hydroxylase 유전자의 다중정렬 분석결과를 토대로 상동성이 높은 부위의 염기서열에 상응하는 degenerate primer를 사용하여 RT-PCR을 행하고, 고유한 염기서열을 가진 partical cDNA clone 14개를 선발했다. 기존에 ecdysteroid 생합성에 관여한다고 알려진 cytochrome P450 family 유전자들과의 염기 및 아미노산 서열의 상동성을 분석한 결과, 선발된 cDNA 중 6개가 cytochrome P45O fumily 유전자들과 상동성이 있는 것으로 나타났다. 이 중에서 4개의 clone은 곤충의 ecdysone 20-hydroxylase 유전자와도 상동성이 높아 ecdysteroid 생합성에 관련된 유전자로 추정되었다.

• 한국식품영양과학회지
• /
• 제27권2호
• /
• pp.319-325
• /
• 1998

The purpose of this study was to investigate the effect of green tea catechin on microsomal mixed function oxidase(MFO) system of kidney and brain in streptozotocin(STZ) induced diabetic rats. Sprague-Dawley male rats weighing 140$\pm$10g were randomly assigned to one control and three STZ-diabetic groups. Diabetic groups wer classified to DM-0C(catechin 0%/kg diet), DM-0.5C (catechin 0.5%/kg diet), and DM-1.0C(catechin 1%/kg diet) according to the level of catechin supplementation. Diabetes were experimentally induced by intravenous administration of 55mg/kg body weight of STZ in citrate buffer(pH 4.3) after 4 weeks feeding of three experimental diets. Animals were sacrificed at the sixth day of diabetic state. The contents of cytochrome P450 in kidney were increased by 77, 42, 49% in DM-0C, DM-0.5C and DM-1.0C groups, respectively, than normal group. The contents of cytochrome P450 in brain were increased by 43% in DM-0C group than normal group, but those of DM-0.5C and DM-1.0C groups were similar to that of normal group. The contents of cytochrome b5 in kidney were increased by 78, 38, 49% in DM-0C, DM-0.5C and DM-1.0C groups, respectively, than normal group. Meanwhile, the contents of cytochrome b5 in brain were not significantly different among all groups. The activities of NADPH-cytochrome P450 reductase in kidney of DM-group were increased by 27% than normal group, but those of DM-0.5C and DM-1.0C groups were 13 and 15% lower than that of DM-0C group. The activities in brain were also increased by 31% in DM-0C group, but those of DM-0.5C and DM-1.0C groups were similar to than of normal group. Levels of TBARS (thiobarbituric acid reactive substance) in kidney were increased by 147, 60 and 59% in DM-0C, DM-0.5C, and DM-1.0C groups, respectively, compared with normal group, but those of DM-0.5C and DM-1.0C groups were 36, 35% lower than that of DM-0C group. Meanwhile, the levels of TBARS in brain were not significantly different among four groups. These results indicate that dietary catechins in green tea play a powerful antioxidant role in reducing the lipid peroxidation enhanced by activation of MFO system in STZ-induced diabetes.

• 통합자연과학논문집
• /
• 제15권2호
• /
• pp.63-72
• /
• 2022

To elucidate the neurochemical characteristics of the midbrain periaqueductal gray (PAG), the distribution patterns of several neuroanatomical markers within the PAG were compared. Immunohistochemical staining for the intracellular calcium binding proteins including calbindin, calretinin, and parvalbumin and histochemical staining for cytochrome oxidase, acetylcholinesterase, and NADPH-diaphorase were performed in. Each chemical substance were localized in the specific subregions within PAG. Calbindin- immunoreactivity were selectively distributed in the dorsolateral PAG, the ventral half of lateral PAG, the ventralateral PAG, and supraoculomotor cap (Su3C) nucleus. Distribution of calretinin-immunoreactivity were generally similar with that of clabindin, but showed relatively low subregional selectivity. Parvalbumin-immunoreactivity was very poor within the PAG. High reactivity of cytochrome oxidase were found in the dorsomedial PAG and the lateral half of lateral PAG, in which calbindin- and calretinin-immunoreactive perikarya were scarcely observed. Acetylcholinesterase distribution was similar with that of cytochrome oxidase, and the difference was in the additional marking of of Su3C with acetylcholinesterase. Results of the present study provides data for the further subdivisions of the territory of the PAG compared to the presently accepted subregions within the PAG.

• Archives of Pharmacal Research
• /
• 제25권6호
• /
• pp.940-945
• /
• 2002

The present study was done to determine the effect of trolox C, a hydrophilic analogue of vitamin E, on hepatic injury, especially the alteration in cytochrome P-450 (CYP)-dependent drug metabolism during ischemia and reperfusion (I/R). Rats were subjected to 60 min of hepatic ischemia and 5 h of reperfusion. Rats were treated intravenously with trolox C (2.5 mg/kg) or vehicle (PBS, pH 7.4), 5 min before reperfusion. Serum alanine aminotransferase and lipid peroxidation levels were markedly increased after I/R. This increase was significantly suppressed by trolox C. Cytochrome P-450 content was decreased after I/R but was restored by trolox C. There were no significant differences in ethoxyresorufin O-dealkylase (CYP 1A1) and methoxyresorufin O-dealkylase (CYP 1A2) activities among any of the experimental groups. Pentoxyresorufin O-dealkylase (CYP 2B1) activity was decreased and aniline p-hydroxylase (CYP 2E1) activity was increased after I/R. Both these changes were prevented by trolox C. Our findings suggest that trolox C reduces hepatocellular damage as indicated by abnormalities in microsomal drug-metabolizing function during I/R, and that this protection is, in part, caused by decreased lipid peroxidation.

• 한국어류학회지
• /
• 제18권1호
• /
• pp.12-19
• /
• 2006

본 연구에서는 극동지역 Oryzias latipes의 지리적 분포와 미토콘드리아 cytochrome b (cyt b)의 유전적 다양성과의 관계를 밝히기 위해 전국 9개의 지역에서 53개체를 채집하여 얻은 cyt b 염기서열과 GenBank에 등록되어있던 117개의 데이터를 종합하여 이를 142개의 haplotype으로 새로이 변환하였고, 이의 계통수를 작성 분석하였다. 분석 결과 극동지역 송사리는 3개의 haplogroup (A, B, C)으로 나뉘어지며, haplogroup A는 남한의 남한아지역을 중심으로 남한 전역에 분포하며, haplogroup B는 중국와 남한의 서한아지역에 걸쳐 분포하며, haplogroup C는 일본에만 분포하였다. Haplogroup A는 haplogroup B와 한반도내에서 지리적으로 가깝게 분포하고 있으나 계통적으로는 상당한 거리를 가지고 뚜렷하게 구별되었다. haplotype의 분지양상은 선행된 연구결과와 거의 일치하였다.

• Journal of the Korean Wood Science and Technology
• /
• 제38권2호
• /
• pp.135-139
• /
• 2010

Reticulitermes speratus is commonly found in Asia, including Korea and Japan. We recently analyzed the 5' region of mitochondrial cytochrome c oxidase subunit I to perform a phylogenetic analysis of R. speratus KMT1, isolated in Seoul, Korea. Our results, using COXI, suggest that the taxonomy of R. speratus should be reconsidered with regard to the subgenus group. A similar phylogenetic analysis by COXI and COXII demonstrated the reliability of COXI genetic information in a molecular phylogenetic analysis of termites.