• Title/Summary/Keyword: cytochrome $c_3$

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The First Human Case of Thelazia callipaeda Infection in Vietnam

  • De, Nguyen Van;Le, Thanh Hoa;Chai, Jong-Yil
    • Parasites, Hosts and Diseases
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    • v.50 no.3
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    • pp.221-223
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    • 2012
  • A 26-year-old man residing in a village of Thai Nguyen Province, North Vietnam, visited the Thai Nguyen Provincial Hospital in July 2008. He felt a bulge-sticking pain in his left eye and extracted 5 small nematode worms by himself half a day before visiting the hospital. Two more worms were extracted from his left eye by a medical doctor, and they were morphologically observed and genetically analyzed on the mitochondrial cytochrome c oxidase 1 gene. The worms were 1 male and 1 female, and genetically identical with those of Thelazia callipaeda. By the present study, the presence of human T. callipaeda infection is first reported in Vietnam.

New record of limnoriid and asellote species(Crustacea: Malacostraca: Isopoda) from South Korea

  • Song, Ji-Hun;Min, Gi-Sik
    • Journal of Species Research
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    • v.6 no.3
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    • pp.237-240
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    • 2017
  • During a scientific survey of Korean isopod fauna, we discovered two marine isopods, Limnoria segnoides Menzies, 1957 and Munna japonica Shimomura and Mawatari, 2001, which are new records to South Korea. Limnoria segnoides and M. japonica were collected by SCUBA diving from the subtidal zone of Jeju Island and Yangyang-gun in South Korea, respectively. In this paper, we provide diagnosis and illustrations of the two isopods. Additionally, we provide GenBank accession numbers of partial sequences of mitochondrial cytochrome c oxidase subunit 1 (CO1) of two species.

DNA Barcoding of Boccardiella hamata (Annelida: Polychaeta: Spionidae) in South Korea

  • Lee, Geon Hyeok;Yoon, Seong Myeong;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • v.36 no.3
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    • pp.268-273
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    • 2020
  • A spionid polychaete, Boccardiella hamata (Webster, 1879) has been found from mud in crevices between the shells of oysters and adherent substrates in South Korea. The sequences of mitochondrial DNA (mtDNA) cytochrome c oxidase subunit 1 (CO1), 16S ribosomal DNA (16S), and the nuclear 18S ribosomal DNA (18S) from Korean individuals of Boccardiella hamata were determined in the present study. The molecular analysis based on the 18S rRNA gene sequences showed clear separation among the spionid polychaete species, and the sequences of Korean and Japanese individuals are completely identical. The morphological diagnosis and photographs of B. hamata are also provided.

New Record of Kellicottia bostoniensis and Redescription of Two Freshwater Rotifers from Korea (Rotifera: Monogononta)

  • Yang, Hee-Min;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • v.36 no.3
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    • pp.222-227
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    • 2020
  • In this study, we identified three monogonont rotifers from South Korea: Kellicottia bostoniensis (Rousselet, 1908), Trichocerca tenuior (Gosse, 1886), and Lepadella triptera (Ehrenberg, 1830). The distribution records of K. bostoniensis were mainly located in the Nearctic, Neotropic and Western Palearctic regions. After Japan, this is the second record of it in Asia. Trichocerca tenuior and Lepadella triptera have already been recorded in Korea, but the data of two species were insufficient in previous study. Here, we describe the morphological characteristics of the three species and the trophi structures of K. bostoniensis and T. tenuior. This study is the first to characterize the trophi structure of K. bostoniensis, observed using a scanning electron microscope. In addition, we have determined the partial cytochrome c oxidase subunit 1 (COI) and 18S rRNA gene sequences of T. tenuior and L. triptera for their DNA barcodes.

DNA Barcoding of Six Diogenid Species (Crustacea: Decapoda: Paguroidea) in Korea

  • Jung, Jibom;Kim, Won
    • Animal Systematics, Evolution and Diversity
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    • v.35 no.4
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    • pp.182-185
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    • 2019
  • In this study, cytochrome c oxidase subunit I(COI) sequences of 17 individuals from six Korean diogenid species(i.e., 2 Areopaguristes japonicus, 4 A. nigroapiculus, 3 Paguristes digitalis, 4 P. ortmanni, 3 Diogenes edwardsii, and 1 Ciliopagurus kempfi) were determined and analyzed. The DNA barcoding results of this study were consistent with the morphological identification of these six species. Interspecific variations of COI sequences within six Korean diogenid species exceeded the minimum interspecific variation of diogenid hermit crabs in previous studies. Little intraspecific variation exists except for P. digitalis. This study should facilitate further molecular taxonomy of East Asian diogenids.

Three Feather Mites (Acari: Sarcoptiformes) Isolated from Black-Tailed Godwit, Limosa limosa in Korea

  • Han, Yeong-Deok;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • v.35 no.3
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    • pp.105-113
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    • 2019
  • Feather mites comprise two superfamilies(Analgoidea and Pterolichoidea) and are highly specialized ectosymbionts of birds. To date, this group contains more than 2,500 species worldwide. Fifty-five feather mite species have been reported in Korea, and only one species of genus Alloptes has been recorded from black-tailed godwit Limosa limosa. Three new records of feather mites from the L. limosa in Korea are added in this study: Avenzoaria punctata Gaud, 1972, Bregetovia limosae (Buchholz, 1869), and Montchadskiana buchholzi (Canestrini, 1878). The genus Bregetovia Dubinin, 1951 is also new report for this country. In this paper, we provide the morphological descriptions and illustrations based on the present specimens. Additionally, we determined partial sequences of the mitochondrial cytochrome c oxidase subunit I(COI) from three feather mites as DNA barcodes.

First Record of the Brackish Water Amphipod Jesogammarus (Jesogammarus) hinumensis (Amphipoda: Anisogammaridae) from Korea with DNA Barcode Analysis among Jesogammarus Species

  • Lee, Chi-Woo;Tomikawa, Ko;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • v.35 no.3
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    • pp.151-155
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    • 2019
  • Jesogammarus (Jesogammarus) hinumensis Morino, 1993 was discovered firstly from a brackish water region in Jeju Island, Korea. To identification of the specimens we conducted both of morphological and molecular analyses. This species is characterized by having large eyes and a robust seta on the mandibular palp article 1. The morphology of this Korean specimens was well matched with the original description without variation. The mitochondrial cytochrome c oxidase subunit I(COI) sequences of the present specimens were also completely identical to the sequences of J. (J.) hinumensis collected from the type locality of the species. Thus, we concluded that the Jesogammarus species from Jeju Island is J.(J.) hinumensis, based on both morphological and molecular data.

DNA Barcoding of Scolelepis (Scolelepis) sagittaria (Annelida, Spionidae) in Korea, with a Morphological Variability of the Species

  • Lee, Geon Hyeok;Yoon, Seong Myeong;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • v.38 no.3
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    • pp.144-147
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    • 2022
  • The polychaete Scolelepis (Scolelepis) sagittaria was originally described from Japanese waters and subsequently reported from Korean waters. In this study, we determined for the first time the mitochondrial cytochrome c oxidase subunit I (COI), 16S ribosomal DNA (16S rDNA), and nuclear 18S ribosomal DNA (18S rDNA) sequences of Korean specimens of S. (S.) sagittaria. We also assessed intraspecific variation in the shape of the prostomium of this species based on an examination of 247 individuals. All materials were collected from intertidal sandy beaches of the Korea Strait. The molecular data and morphological observations reported herein will contribute to gaining a better understanding of the taxonomic relationships among members of the genus Scolelepis.

New Records of Four Feather Mites (Acariformes: Astigmata) from Waders (Charadriiformes: Scolopacidae) in Korea

  • Han, Yeong-Deok;Mironov, Sergey V.;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • v.38 no.3
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    • pp.130-139
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    • 2022
  • Four feather mites, Dichobrephosceles actitidis(Canestrini, 1878)(Alloptidae), Limosilichus limosae Vasyukova and Mironov, 1986, L. numeni Vasyukova and Mironov, 1986 (Syringobiidae), and Pomeranzevia numenii (Canestrini, 1878) (Avenzoariidae), have been reported in Korea for the first time. Specimens of D. actitidis and L. limosae were obtained from the common sandpiper, Actitis hypoleucos (Linnaeus, 1758), and the black-tailed godwit, Limosa limosa (Linnaeus, 1758), respectively, while L. numeni and P. numenii were collected from the Eurasian whimbrel, Numenius phaeopus (Linnaeus, 1766). The genera Dichobrephosceles Peterson and Atyeo, 1968, Limosilichus Vasyukova and Mironov, 1986, and Pomeranzevia Dubinin, 1951 are also new records for the fauna of Korea. This paper provides morphological descriptions, illustrations, and partial sequences of mitochondrial cytochrome c oxidase subunit I(COI) for all the recorded species based on the specimens collected in Korea.

First Record of Scolelepis (Scolelepis) daphoinos (Annelida: Polychaeta: Spionidae) in South Korea

  • Lee, Geon Hyeok;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • v.37 no.3
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    • pp.229-234
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    • 2021
  • Scolelepis (Scolelepis) daphoinos is newly reported in Korean fauna. This species can be distinguished from its congeners by the following characteristics: the presence of reddish pigment patches on the posterior part of the prostomium, notopodial postchaetal lamellae that are partially fused to the branchiae, and the presence of only the bidentate hooded hooks. The morphological diagnosis and photographs of S. (S.) daphoinos are provided. The partial mitochondrial cytochrome c oxidase subunit I (COI), 16S ribosomal DNA(16S rDNA), and the nuclear 18S ribosomal DNA (18S rDNA) sequences from Korean specimens of S. (S.) daphoinos were determined. Species identification was supported by a comparison of DNA barcode sequences of COI and 16S rDNA with morphological examination from the specimens of type locality, China.