• Development and Reproduction
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• v.20 no.2
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• pp.135-140
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• 2016

Genomic DNAs isolated from crucian carp of four rivers, belonging to the family Cyprinidae was amplified by seven oligonucleotides primers. In the present study, we employed hierarchical clustering method in order to reveal genetic distances and variations. Crucian carp was acquired from Hangang river (CAH), Geumgang river (CAG), Nakdonggang river (CAN) and Yeongsangang river (CAY). The primer BION-12 generated the most loci (a total of 50) with an average of 10 in the CAY population. The primer BION-10 generated the least loci (a total of 19), with an average of 3.8 in the CAG population, in comparison to the other primers used. Seven oligonucleotides primers made 16.7 average no. per primer of specific loci in the CAH population, 7.4 in the CAG population, 8.6 in the CAN population and 0.9 in the CAY population, respectively. The specific loci generated by oligonucleotides primers revealed inter-individual-specific characteristics, thus disclosing DNA polymorphisms. The dendrogram obtained by the seven oligonucleotides primers indicates four genetic clusters. The genetic distance that displayed significant molecular differences was between individuals no.06 and no.08 from the CAG population (genetic distance = 0.036), while the genetic distance among the five individuals that displayed significant molecular differences was between individuals no.08 and no.09 from the CAG population (genetic distance = 0.088). With regard to average bandsharing value (BS) results, individuals from CAY population ($0.985{\pm}0.009$) exhibited higher bandsharing values than did individuals from CAH population ($0.779{\pm}0.049$) (P<0.05). Relatively, individuals of CAY population were fairly closely related to that of CAN location (genetic distance between two populations<0.016).

• Korean Journal of Ecology and Environment
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• v.42 no.3
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• pp.394-403
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• 2009

The fish fauna and community structures were investigated at 15 stations in the midupper region of the Seomjin River watershed from August in 2008 to April in 2009. During the studying period, 42 species belonging to 11 families were collected. Dominant family in the all sites was Cyprinidae (28 species, relative abundance: 66.7%) during the study. Total of 42 species (primary freshwater: 40 species, peripheral freshwater: 2 species) were found with the primary freshwater fishes being the highest. Dominant species was Zacco platypus (22.3%), and subdominant species was Coreoferea herzi (10.8%). Also, Pseudogobio esocinus (9.0%) and Carassius auratus (5.8%) were numerous. There were 17 Korean endemic species (40.5%), including Rhodeus ocellatus, Acheilgnathus koreensis, A. somjinensis, Coreoleuciscus splendidus and Sarcocheillichthys variegatus wakiyae. Among them, Acheilgnathus somjinensis was identified for designation required to protect under a wildlife category. In conclusion, we could define that the Seomjin River watershed has a very good water conditions as habitat of fish.

• Applied Microscopy
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• v.31 no.1
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• pp.85-90
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• 2001

The ultrastructures of the fertilized egg envelope from long nose barbel, Hemibarbus longirostris belong to Cyprinidae was studied using scanning and transmission electron microscopes. The fertilized egg was adhesive type, have a single micropyle resembling the pathway of sperm in the area of the animal pole. An outer surface of the fertilized egg envelope was arranged by adhesive structures irregularly. In section of fertilized egg, the egg envelope consists of two layers, an outer adhesive twofold layer with mushroom-like cluster and an inner lamellae layer consisting of four layers. These ultrastructural characters of fertilized egg envelope from long nose barbel can be utilized in taxonomy of teleost.

• Korean Journal of Ichthyology
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• v.32 no.3
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• pp.182-190
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• 2020

The endangered Pseudopungtungia tenuicorpa were reintroduced to the Jojongcheon Stream in the Hangang River 2011~2012 as part of the restoration project. Since then, the restoration project has been evaluated as a failure because the P. tenuicorpa were not collected from the discharge site 2014~2016. This study was conducted 2017~2019 to verify that the P. tenuicorpa restoration project failed. Six locations were selected, including the discharge site, and the fish collection method used skimming nets, cast nets, long bag set nets, and scuba diving. In the survey results, 13 individuals were collected in a 0.5 km section, including the discharge station. The P. tenuicorpa habitat was approximately at a water depth of 0.5 m to 2 m in run water flow with the boulders and rocks. The age of the collected P. tenuicorpa were 0+ to 3-years-old, and the generation alternation was occurring stably. Thus, the P. tenuicorpa restoration project was evaluated as partially-successful, as the P. tenuicorpa, re-introduced to the Jojongcheon Stream, settled in its habitat with stable generation alternation.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.5
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• pp.436-448
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• 1984

The taxonomy of gudgeons of the subfamily Gobioninae in Korea is reviewed and keys to supraspecific taxa (subfamilies and genera) of the subfamily Gobioninae are provided, as well as the synonyms and distribution of the species or subspecies. Abbottina springeri, poorly known in Korea is redescribed with the extention of its known morphological characters and geographical distribution. Squalidus sp. occurring rivers of the Yongdokoship, the Hyongsan, and the Hoeya in the Gyongsangbugdo and in the Gyongsangnamdo differs from three previously described species from Korea in the numbers and structure of lateral line scales, color patterns on the body sides, and eye diameter etc. The genus name of Gonoprcktopterus mylodon should be transferred from Gonoproktopterus to Hemibarbus, because the species is more similar to Hemibarbus longirostris or Belligobio eristrigma than to Gonoproktopterus, an Indian genus of Barbinae. The korean gudgeons of the subfamily Gobioninae are grouped five possible phyletic series, as follow ; Hemibarbus, Pseudorasbora, Sarcocheilichthys, Gnathopogon, and Pseudogobio group. The genus Gobiobotia is not placed in the subfamily Gobioninae but in the subfamily Gobiobotinae in relation to 3 pairs of mental barbels and some osteological characters.

• Korean Journal of Ichthyology
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• v.26 no.4
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• pp.249-258
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• 2014

To examine the taxonomic status of Acheilognathus sp. specimens from the Geum River, morphological and genetical characteristics of A. sp., A. yamatsutae and A. majusculus were investigated and compared in detail. Specimens of A. sp. could be distinguished from the other two species by the combination of some morphological characters such as nuptial color, vertebrae, gillrakers and etc. Males of A. sp. had red bands on the outer margin of dorsal and anal fins and a white band on the outer margin of ventral fin in breeding season. A. sp. had larger maximum body length and somewhat more vertebrae than A. yamatsutae, and had fewer gillrakers than A. majusculus. A. sp. appeared as a monophyletic group with A. majusculus and A. cyanostigma based on genetic analysis. In addition, it had even more close relationship with other congeners than A. yamatsutae. Therefore it is presumed that A. sp. from the Geum River may be a distinct species in genus Acheilognathus.

• Korean Journal of Ichthyology
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• v.20 no.1
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• pp.7-12
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• 2008

Study on spermatid and spermatozoon of a Korean endemic freshwater fish, Microphysogobio yaluensis, has been carried out by transmission electron microscope. Spermiogenesis involves conspicuous modifications intercelluar movement such as centrioles and mitochondria migration, nuclear depression and structural changes for instance chroatin condensation, excess of cytoplasm. The mature spermatozoa are similar to those of other cyprinids as follows: a spherical nucleus with a shallow nuclear fossa, a short midpiece containing several mitochondria and encircling the basal body of the flagellum. However there are some differences in the orientation of the centrioles,the number of the mitochondria and distribution of vesicles from other cyprinids. It could be concluded that the M. yaluensis spermiogenesis belongs to type 2, and its mature spermatozoon is charaterized by a unique feature which may provide a useful systematic character.

• Korean Journal of Ichthyology
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• v.30 no.2
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• pp.71-74
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• 2018

The karyotype analysis of an endangered freshwater fish, Microphysogobio koreensis, was performed to obtain basic data for phylogenetic information. To carry out our study, 4 specimens were collected in Seomjingang River and Nakdongang River and its kidney was treated by flame-drying method. The chromosome number of this species demonstrated 50 diploid chromosomes, with two populations of M. koreensis not significantly different. The karyotype revealed 2n=26m+24sm, consisting of 26 metacentric (m) and 24 submetacentric (sm) chromosomes with the total fundamental arm number determined as FN=100. Total arm length and arm ratio of the chromosomes were $1.44{\sim}2.68{\mu}m$ and 1.27~2.27, respectively. The karyotype of M. koreensis was first reported in this study.

• Korean Journal of Ichthyology
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• v.31 no.4
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• pp.241-248
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• 2019

The freshwater fish fauna for the whole water system of Geogeumdo Island was surveyed at a total 10 sites from March 2018 to February 2019. During the surveyed period, 13 species belonging to 5 families and 4 orders were collected. Cyprinidae (68.84%) and Gobiidae (18.21%) were the dominant family, and the dominant species was Zacco temminckii (53.71%), and the subdominant species was Rhynchocypris oxycephalus (10.78%). Kichulchoia brevifasciata (1.27%) that endangered species were found in this study, but the habitat was confirmed in one site in Sinpyeongcheon compared to previous literature. Three endemic species of Korea were found in this island such as Iksookimia hugowolfeldi (5.07%), Liobagrus somjinensis (2.99%) and K. brevifasciata (1.27%). According to analysis of fish community structure based on average diversity, evenness index, dominance and richness indices in Geogeumdo Island were 1.666, 0.649, 0.645 and 1.713, respectively.

• The Korean Journal of Zoology
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• v.34 no.4
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• pp.571-584
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• 1991

잉어과(Gyprinidae)의 Zocco속 어류 4종과 Candidia속 어류 1종에 대한 종간 유연관계와 종분화 연대측정 및 이들의 지질학적 분포경로를 밝히기 위하여 한국, 일본 및 대만에서 채집된 개체를 대상으로 전기영동법에 의한 유전자 분석을 하였다. 각 종의 지역별(한국, 일본, 대만) 집단간 유전적 유연관계를 분석한 결과 평균 유전적 근연치는 90% 이상이었다. Z. temmincki의 경우 일본집 단들은 한국의 A-type 집단과는 유연관계가 가까왔으나 한국의 B-type과는 유전적 차이가 현저하러다. Z. PlaD여5의 경우 한국집단과 일본집단사이의 유연관계는 S = 0.852였고, 한국집단과 대만집단 사이는 5 = 0.672, 일본집단과 대만집단 사이는 S = 0.751로서 지리적으로 현저한 차이를 업였다. Z. pochycephalus 3개 집단간의 유전적 근인치는 S = 0.963이었고 Candidia borbota 2개 집단간은 S = 0.946이었다. 종간의 유전적 근연환계를 비교한 결과 Candidia borbota와 Z. temmincki사이는 S = 0.608, Z. pluDpus와 1. pachycephalus사이는 S = 0.612였으나, Z. temmincki와 Z. platypus사이는 S = 0.441, Z. temmincki 와 Z. pochycepholus 사이는 S = 0.350이었고, Z. plotpus와 Condfda barbata사이는 S = 0.328로서 이들 사이에는 현저한 유전적 차이가 있었다. 각 종간의 롱분화 연대를 추산한 결과 이들은 약 480만년 전인 Pliocene 초기에 공통 조상종에서 분화하여 Z. temmincki, Candidia borbato group과 Z. plotypus, Z. pochycepholus group으로 분리되었고 약 260만년 전인 Pliocene 후기에 Z. temminc소와 Candidia borbota로 분화되었다고 추산되며 약 80만년 전인 Pleistocene시기에 남 temmincki B-type에서 h-type이 분화되었다짙 여겨진다. 한편 또 다른 한 단opP은 약 230만년 전인 열iocene후기에 대만 지역의 Z. plotypas에서 Z. pochvcepholus가 분화된 후 현재에 이르렀다고 추정된다. Z. platypus는 약150만년 이전인 초기 Pleistocene시기에 대만지역에서 한국 및 일본집단으로 분리되었다고 보며 이들 한국집단과 일본집단은 약 50만년 전 Pleistocene의 Middle기에 고황하 수계를 거쳐 현재의 분포 상황에 이르렀다고 여겨진다. 한편 대. temmin체과의 B-type에서 저온 적응으로 분화되었다고 추측되는 A-type은 약 20만년 전인 Pleistocene의 Riss기에 역시 고황하 수계를 통하여 한국과 일본으로 분포하여 현재에 이르렀다고 사료된다.