• Applied Biological Chemistry
• /
• v.48 no.1
• /
• pp.93-97
• /
• 2005

Three antioxidants, Sa-1, Sa-2 and Sa-3, were isolated from the MeOH extract of the mushroom Sarcodon aspratus through EtOAc extraction, silica gel and Sephadex LH-20 column chromatography, and $C_{18}$ HPLC. The structures of the compounds were determined mainly by NMR and mass spectroscopic data analyses. Sa-1, Sa-2 and Sa-3 were identified as diketopiperazines, and their structures were determined to be cyclo(prolyl-valyl), cyclo(prolyl-leucyl) and cyclo(prolyl-isoleucyl), respectively. This is the first time that these compounds were isolated from this mushroom. They showed antioxidant activity by scavenging DPPH radical $(EC_{50}\;0.15{\sim}0.18\;mM)$ and superoxide radical $(EC_{50}\;0.21{\sim}0.24\;mM)$.