• Proceedings of the Korean Society of Crop Science Conference
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• 2019.09a
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• pp.22-22
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• 2019

Green stem disorder (GSD) of soybean (Glycine max (L.) Merr.) is characterized by delayed senescence of stems with normal pod ripening and seed maturation (Hobbs, 2006). GSD complicates harvesting of soybeans by significantly increasing the difficulty in cutting the affected plants. There is also the potential for moisture in the stems to be scattered on the seed, reducing the grade and storability of the seed. Not only the cause of GSD is yet unknown, but also GSD cannot be evaluated until maturity, therefore the method to evaluate GSD in early growth stage with high sensitivity is necessary. In previous studies, it has been reported that vegetative storage protein (VSP) accumulates and the syndrome of GSD appears in soybean after depod treatment (Fischer, 1999). Soybean VSP is a storage protein which is abundant in young sink leaves and degraded during seed fill (Wittenbach, 1982). Hence, we have established a system to quantify VSP of high sensitivity by using standard protein made by genetically transformed E. coli and specific antibody against VSP, and studied the relationship between VSP and GSD, by depod experiment and drought/excess wet experiments. The result of depod experiment with the cultivar 'Yukihomare' was the same with the previous studies, VSP accumulated much more than control and the syndrome of GSD appeared in soybean in depod treatment. Drought and excess wet had different impact on GSD. Excess wet caused GSD of the cultivar 'Tachinagaha (GSD susceptible)', while drought caused a little syndrome of GSD in the cultivar 'Touhoku 129 (GSD resistant)'. The accumulation of VSP differed between the two cultivars over time. In conclusion, the accumulation of VSP came along with the emergence of GSD. Different cultivars showed different response to drought and excess wet. In the future, it is expected that the dynamics of VSP will be elucidated in detail, leading to the development of early diagnosis technology for green stem disorder and the elucidation of mechanism of soybean GSD.

• Journal of Ginseng Research
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• v.45 no.1
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• pp.58-65
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• 2021

Background: Panax ginseng, as one of the most widely used herbal medicines worldwide, has been studied comprehensively in terms of the chemical components and pharmacology. The proteins from ginseng are also of great importance for both nutrition value and the mechanism of secondary metabolites. However, the proteomic studies are less reported in the absence of the genome information. With the completion of ginseng genome sequencing, the proteome profiling has become available for the functional study of ginseng protein components. Methods: We optimized the protein extraction process systematically by using SDS-PAGE and one-dimensional liquid chromatography mass spectrometry. The extracted proteins were then analyzed by two-dimensional chromatography separation and cutting-edge mass spectrometry technique. Results: A total of 2,732 and 3,608 proteins were identified from ginseng root and cauline leaf, respectively, which was the largest data set reported so far. Only around 50% protein overlapped between the cauline leaf and root tissue parts because of the function assignment for plant growing. Further gene ontology and KEGG pathway revealed the distinguish difference between ginseng root and leaf, which accounts for the photosynthesis and metabolic process. With in-deep analysis of functional proteins related to ginsenoside synthesis, we interestingly found the cytochrome P450 and UDP-glycosyltransferase expression extensively in cauline leaf but not in the root, indicating that the post glucoside synthesis of ginsenosides might be carried out when growing and then transported to the root at withering. Conclusion: The systematically proteome analysis of Panax ginseng will provide us comprehensive understanding of ginsenoside synthesis and guidance for artificial cultivation.

• ALGAE
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• v.35 no.4
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• pp.389-404
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• 2020

Red algal fertilization is unusual and offers a different model to the mechanism of intracellular transport of nuclei and polyspermy blocking. A female carpogonium (egg) undergoes plasmogamy with many spermatia (sperm) simultaneously at the receptive structure, trichogyne, which often contains numerous male nuclei. The pattern of selective transport of a male nucleus to the female nucleus, located in the cell body of the carpogonium, remain largely unknown. We tracked the movement of spermatial nuclei and cell organelles in the trichogyne after plasmogamy using time-lapse videography and fluorescent probes. The fertilization process of Bostrychia moritziana is composed of five distinctive stages: 1) gamete-gamete binding; 2) mitosis in the attached spermatia; 3) formation of a fertilization channel; 4) migration of spermatial nuclei into the trichogyne; and 5) cutting off of the trichogyne cytoplasm from the rest of the cell after karyogamy. Our results showed that actin microfilaments were involved in the above steps of fertilization, microtubules are involved only in spermatial mitosis. Time-lapse videography showed that the first ("primary") nucleus which entered to trichogyne moved quickly to the base of carpogonium and fused with the female nucleus. The transport of the primary male nucleus to the egg nucleus was complete before its second nucleus migrated into the trichogyne. Male nuclei from other spermatia stopped directional movement soon after the first one entered the carpogonial base and oscillated near where they entered trichogyne. The cytoplasm of the trichogyne was cut off at a narrow neck connecting the trichogyne and carpogonial base after gamete nuclear fusion but gamete binding and plasmogamy continued on the trichogyne. Spermatial organelles, including mitochondria, entered the trichogyne together with the nuclei but did not show any directional movement and remained close to where they entered. These results suggest that polyspermy blocking in B. moritziana is achieved by the selective and rapid transport of the first nucleus entered trichogyne and the rupture of the trichogyne after gamete karyogamy.

• Journal of Korean Society of Forest Science
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• v.100 no.4
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• pp.548-557
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• 2011

REDD is recognized as a cost-effective and the most appropriate way of managing global GHG emissions. It is negotiated in a global context under the UNFCCC. The main issues of the REDD mechanism are divided into two parts: 1) developing forest carbon verification and measurement system for reducing emissions from deforestation and forest degradation, 2) establishing an appropriate system of incentives for developing countries. REDD can provide stability in carbon offset credits. However, the most important task is to establish a clear legal framework and appropriate governance structures with relevant countries. There is a wide opportunities for Republic of Korea to take a lead in cutting off greenhouse gasses in the Post-Kyoto period.

• Geomechanics and Engineering
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• v.28 no.3
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• pp.283-298
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• 2022

Landslides triggered by the combination of heavy precipitation and anthropological disturbance in hilly areas cause severe damage to human lives, properties, and infrastructure constructions. A comprehensive investigation of the influencing factors and failure mechanisms of landslides are significant for disaster mitigation and prevention. This paper utilized the combination of detailed geological investigation, physical experimental testing as well as numerical modelling to determine the failure mechanism, and proposed a countermeasures of the Lantian landslide occurred on 2, July 2017. The results reveal that the Lantian landslide is a catastrophic reactivated slide which occurred in an active tectonic region in Southwest China. Because of the unique geological settings, the fully to highly weathered basalts in the study area with well-developed fractures favored the rainwater infiltration, which is the beneficial to slide reactivation. Engineering excavation and heavy precipitation are the main triggering factors to activate the slide motion. Two failure stages have been identified in the landslide. The first phase involves a shallow mass collapse originated at the upper slopes, which extends from the road to platform at rear part, which is triggered by excavation in the landslide region. Subjected to the following prolonged rainfall from 19 June to 2 July, 2017, the pore water pressure of the slope continually increased, and the groundwater table successively rise, resulting in a significant decrease of soil strength which leads to successive large-scale deep slide. Thereinto, the shallow collapse played a significant role in the formation of the deep slide. Based on the formation mechanisms of the landslide, detailed engineering mitigation measures, involving slope cutting, anchor cable frame, shotcrete and anchorage, retaining wall and intercepting ditch were suggested to reduce the future failure risk of the landslide.

• Atmosphere
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• v.33 no.2
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• pp.125-154
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• 2023

Since the Korean Meteorological Society was organized in 1963, the climate dynamics fields have been made remarkable progress. Here, we documented the academic developments in the area of climate dynamics performed by members of Korean Meteorological Society, based on studies that have been published mainly in the Journal of Korean Meteorological Society, Atmosphere, and Asia-Pacific Journal of Atmospheric Sciences. In these journals, the fundamental principles of typical ocean-atmosphere climatic phenomena such as El Niño, Madden-Julian Oscillation, Pacific Decadal Oscillation, and Atlantic Multi-decadal Oscillation, their modeling, prediction, and its impact, are being conducted by members of Korean Meteorological Society. Recently, research has been expanded to almost all climatic factors including cryosphere and biosphere, as well as areas from a global perspective, not limited to one region. In addition, research using an artificial intelligence (AI), which can be called a cutting-edge field, has been actively conducted. In this paper, topics including intra-seasonal and Madden-Julian Oscillations, East Asian summer monsoon, El Niño-Southern Oscillation, mid-latitude and polar climate variations and some paleo climate and ecosystem studies, of which driving mechanism, modeling, prediction, and global impact, are particularly documented.

• KEPCO Journal on Electric Power and Energy
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• v.6 no.3
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• pp.305-313
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• 2020

The use of cable tunnels for electric power transmission as well as their construction in difficult conditions such as in subsea terrains and large overburden areas has increased. So, in order to efficiently operate the small diameter shield TBM (Tunnel Boring Machine), the estimation of advance rate and development of a design model is necessary. However, due to limited scope of survey and face mapping, it is very difficult to match the rock mass characteristics and TBM operational data in order to achieve their mutual relationships and to develop an advance rate model. Also, the working mechanism of previously utilized linear cutting machine is slightly different than the real excavation mechanism owing to the penetration of a number of disc cutters taking place at the same time in the rock mass in conjunction with rotation of the cutterhead. So, in order to suggest the advance rate and machine design models for small diameter TBMs, an EPB (Earth Pressure Balance) shield TBM having 3.54 m diameter cutterhead was manufactured and 19 cases of full-scale tunneling tests were performed each in 87.5 ㎥ volume of artificial rock mass. The relationships between advance rate and machine data were effectively analyzed by performing the tests in homogeneous rock mass with 70 MPa uniaxial compressive strength according to the TBM operational parameters such as thrust force and RPM of cutterhead. The utilization of the recorded penetration depth and torque values in the development of models is more accurate and realistic since they were derived through real excavation mechanism. The relationships between normal force on single disc cutter and penetration depth as well as between normal force and rolling force were suggested in this study. The prediction of advance rate and design of TBM can be performed in rock mass having 70 MPa strength using these relationships. An effort was made to improve the application of the developed model by applying the FPI (Field Penetration Index) concept which can overcome the limitation of 100% RQD (Rock Quality Designation) in artificial rock mass.

• Journal of Environmental Policy
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• v.6 no.1
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• pp.41-60
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• 2007

Attempts were made to define the elements of debates, impact of decisions of the Access to Genetic Resources and Benefit-sharing(ABS) of the Conference of the Parties(COP) to the Convention on Biological Diversity(CBD) In Korea. Providing policy suggestions to cope with ABS, a cross-cutting issue of the meetings of the COP, was also undertaken. Meetings concerning ABS deal with several key matters such as an international regime, which is a legally binding implementation tool of the Bonn Guidelines, an international certificate of genetic resources' origin/source/legal provenance, and disclosure of origin of genetic resources, compliance measures with prior informed consent of the Contracting Parties providing such resources and with mutually agreed terms on which access was granted. Developing countries, rich in biodiversity and genetic resources, use the CBD as a major tool to maximize their national profits. They demand for national sovereign rights for the genetic resources and indigenous communities providing associated traditional knowledge. At the meetings of the COP, in addition, they requested that developed countries should transfer technologies and provide a financial mechanism for resource conservation to them. On the contrary, the developed countries argue that facilitating access to genetic resources is essential for scientific research and development, and that both Intellectual Property Rights and biotechnology using genetic resources should be protected to maximize their national benefits. Decisions of the COP concerning the Bonn Guidelines and compliance measures with ABS will affect on various socioeconomic fields of Korea, a country which is short of genetic resources. Especially, the importation of genetic resources and land development which might damage genetic resources will be limited seriously. Consequently, overall expenses will increase for the securing genetic resources from the foreign countries and developing biotechnology for conservation and sustainable uses of genetic resources. To minimize the adverse impacts, we endeavor to establish our clear standpoint and to lead the international trends, which are favorable for us. In order to achieve these objectives, government needs i) to proceed researches to lead the international ABS debates actively and to prepare the expected decisions of the future meetings of the COP, ii) to establish a national implementation plan to cope with the ABS and its related decisions, iii) to examine and improve the efficiencies of the national implementation plan with a proper monitoring system, and iv) cope with the other international meetings including the meetings of Trade Related Intellectual Properly Rights and International Treaty on Plant Genetic Resources for Food and Agriculture actively.

• Journal of Korean Society of Forest Science
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• v.105 no.1
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• pp.42-47
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• 2016

To examine the natural regeneration in the natural mixed-broadleaved forest, flowering, fruiting, seed-fall, and seed viabilities of Acer pseudosieboldianum (Ap) were investigated in Mt. Jungwang, Gangwon-do, from 2009 to 2015. The flower of Ap consisted many male and bisexual flowers on the corymb. Flowering dates are differed between sex morph in the same inflorescence. Stamens are stop growing and disappeared after pollinated pistil begin to grow in bisexual flowers, and male flowers have vestial pistil. The flowers of Ap might be pollinated by Apis mellifera, Andrenidae spp. and Syrphinae spp. Ap had some mechanism to prevent from self pollination with heterodichogamy. Mean annual seedfall of Ap was 70,780 ea/ha (ranged 310~234,840 ea/ha). Annual seedfall of Ap varied severely, and the maximum was about 760 times the minimum. Annual seed production of Ap might be to a normal distribution. Rates of damaged or decayed seeds are highest 59.3%, and those of sound seeds are 23.9%, Those of undeveloped and empty seeds are 9.2% and 7.6%, respectively. The most important factors influencing sound seed production might be the density and activities of insect pollinators and sucking pest in the flowerwing period, middle-late May. Successful regeneration of Ap might be in masting year and on the gap sites with proper conditions to germinate and grow. To understand the natural regeneration of deciduous hardwoods, further study on the characteristics of flowering and fruiting, pre- and post-dispersal seed predation, and annual variation on these factor should be needed.

• Journal of Korean Society of Forest Science
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• v.103 no.2
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• pp.153-158
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• 2014

To examine the natural regeneration in the natural mixed-broadlived forest, flowering, fruiting, seed-fall, and seed viabilities of Acer ukurunduense Trautv. & C.A. Mey. (AU) were investigated in Mt. Jungwang, Gangwon-do, from 2009 to 2013. The flower of AU consisited many male and bisexual flowers on the raceme (unusual panicle) and the arrangement of two sex morphs are differed for each inflorescence. Flowering dates are differed between sex morph in the same inflorescence. Stamens are stop growing and disappeared after pollinated pistil begin to grow in bisexual flowers, and male flowers have vestial pistil. The flowers of AU might be pollinated by Apis mellifera, Thyris fenestrella seoulensis, Cerambycidae sp., Andrenidae sp. and Ctenophora sp., and had some mechanism to prevent feom self pollination. The number of flower buds per inflorescence is 189 on June 8, and that of young samaras per inflorescence is 41.2 on June 21. At last ripened samaras per inflorescence is reduced 33.4 on September 5. Mean annual seedfall of AU was 6,720 ea/ha (ranged 670~17,930). Rates of sound seeds are highest 43.2%, and those of damaged or decayed seeds are 41.8%,. Those of undeveloped and empty seeds are 10.2% and 4.8%, respectively. Successful regeneration of AU might be in masting year and on the gap sites with proper conditions to germinate and grow. To understand the natural regeneration of the species, Genus Acer, further study on the fruiting habit, pre- and post-dispersal seed viability, and annual variation on these factor should be needed.