• 한국연초학회지
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• 제26권2호
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• pp.79-84
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• 2004

During the screening of antiviral substances having inhibitory effects on tobacco mosaic virus (TMV) infection to tobacco plants, we found a bacterial isolate KTGBP10, which was identified as a Stenotrophomonas sp., strongly inhibited the infection of TMV. When the culture filtrate from KTGBP10 was applied on the upper surface of leaves of Xanthi-nc tobacco plants at the same time or 24 hours before TMV inoculation, almost complete inhibition of TMV infection was achieved. And $40\%$ inhibition was shown with application of the culture filtrate to the under surface of leaves. In field trials, transmission of TMV from diseased seedlings to the healthy ones during transplanting work was reduced by $87.1\~92.6\%$ when the culture filtrate or cell suspension was sprayed onto the tobacco seedlings, cv. NC82, 24 hours before transplanting. No toxic effect was observed on the tobacco plants. When the broth filtrate of KTGBP10 was supplied by soaking through the cut-leaves before and/or after virus inoculation, the TMV infection was also inhibited by $50.4\~65.3\%$.

• 한국연초학회지
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• 제27권2호
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• pp.219-225
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• 2005

Characterizing nicotine delivery from tobacco products is important in the understanding of their addictive potential. Most previous studies report total nicotine and have not differentiated between nicotine in its protonated or free-base form. The amount of free nicotine calculated by determining pH and nicotine contents. The pH and nicotine contents in smokeless tobacco product, tobacco products and tobacco leaves were analyzed by Health Canada-Official Method T-310 and CORESTA Recommended Method $N^{\circ}62$. The content of free nicotine was calculated according to the Henderson-Hasselbalch equation and the value of $\alpha_{fb}$(the fraction of nicotine that is in the free base form) by using a pKa value of 8.02 for nicotine. The percentage of free nicotine then was calculated by dividing the free nicotine content by total nicotine content. The pH value and percentage of free nicotine ranged from 5.01 to 5.45 and $0.10\%\;to\;0.27\%$ in cut tobacco and 5.10 to 7.10 and $0.12\%\;to\;10.73\%$ in tobacco leaves, respectively.

• 한국연초학회지
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• 제22권2호
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• pp.184-189
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• 2000

This study was conducted to develop the objective evaluation method for tobacco smoke. The evaluation was carried out by using the data of cut or blended tobacco components, smoke components, electric nose system (ENS), and sensory test. By using the statistical methods, such as cluster analysis, discriminant analysis, factor analysis, correlation analysis, and multiple regression analysis, the relationship among the data of tobacco, smoke, ENS, and sensory evaluation was studied. By the results of cluster analysis, the data from smoke analysis by GC and ENS were able to select the difference of tobacco leaf characteristics. As the results of discriminant analysis, grouping by the components of tobacco leaves and smoke was possible and the results of GC analysis of smoke could be used for discrimination of tobacco leaves. In the results of factor analysis, nicotine, tar, CO, puff No and pH in the smoke were the factors effecting on the tobacco leaf characteristics. From the correlation analysis, aroma, taste, irritation, and smoke volume of sensory test had high relation to tar, p-cresol threonolatone, levoglucosane, and quinic acid- ${\gamma}$ -lactone of smoke. The ENS data showed high efficiency for discriminant analysis and cluster analysis, but it was not good for factor analysis, and correlation analysis. It was possible to estimate tobacco leaves and their blending characteristics by the analytical data of tobacco leaves, smoke, ENS, and sensory test results. By the multiple regression analysis, some correlation among selected chemical components and sensory evaluation were found. This study strongly indicated that the some chemical analysis data was available for the objective evaluation of tobacco sensory attributes.

• 한국연초학회지
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• 제19권1호
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• pp.29-32
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• 1997

This study was carried out to develop a new stalk curing of the horizontal hanging method that would reduce the harvesting and curing labor, and improve its quality of cured leaf than the conventional stalk curing method in burley tobacco. The horizontal hanging stalk-curing method designed from 1988 was compared with the conventional method. The horizontal method shortened the curing Period for 7 days and reduced to 0.3%, while it occurred 9.4% in the vertical method. In the horizontal method, the quality of cured leaf improved 9%, but the contents of chemical components and physical properties were similar to those of vertical method. The labor of stalk-cut and loading in curing facility reduced 28% by the horizontal method. In harvesting and curing process, the horizontal method resulted 12% of labor saving as compared with the vertical method. Key words : horizontal hanging stalk-curing method, vertical method, stalk-curing.

• 한국연초학회지
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• 제20권2호
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• pp.231-237
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• 1998

This study was carried out to investigate the effects of curing methods on the concentration of ammonia during curing in burley tobacco leaves. The air-cured tobacco(KB108; Nicotiana tabacum L.) was grown at Chonju Tobacco Experiment Station in 1998 and the tenth leaves from the top on the stalk were harvested. Half of the harvested leaves were cured in normal air curing facility and the other leaves were cured in excessive curing facility. Stalk cut tobaccos were cured in horizontal curing facility. The leaves were sampled every five days from harvesting time to the end of curing(25 days). Ammonia concentration of leaves increased during curing period with a remarkable increase at yellowing stage. The concentration of ammonia was high in the primed cured leaves, while that of the excessive cured leaves was low. It is considered that the lower increase of ammonia in stalk cured leaves may be caused by the translocation from the leaves to the stalk during curing, while that of excessive cured leaves may be caused by the poor decomposition of protein and amino acid during curing by excessive moisture loss and high temperature condition.

• 한국연초학회지
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• 제1권1호
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• pp.71-77
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• 1979

제조담배의 중량을 엽배합 표준과 잎담배의 팽숭성, 제품제제 그리고 외관품질을 인자로 계산하였다. 계산에 의해 새로이 정한 제품의 중량은 tobacco rod가 60mm인 제품이 760mg으로서 현재의 표준중량 790mg∼830mg보다 5.1 %∼6.8%가 적었으며, 64mm 제품은 820mg으로서 870mg∼900mg보다 5.9%∼9.1%를 줄일 수 있고 56mm 제품은 720mg으로서 770mg∼ 780mg에 비하여 6.9%∼8.6%가적은 값으로서 지금의 제품담배의 중략보다 5∼ 9%를 줄일 수 있음을 보여 주고 있다.

• 한국연초학회지
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• 제5권1호
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• pp.79-85
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• 1983

The greenhouse bulk curing and drying system utilization of the direct solar energy was tested to evaluate that how much fuel could be saved for curing flue-cured tobacco at the Dae Gu Experiment Station (North latitude : $35^{\circ}$49'), in 1979-1982. The air temperature and total radiation were 19.0 to 38.5$^{\circ}C$ and 1311.0 to 1412.7 cal/$\textrm{cm}^2$/day during the 4 replicated curing test, respectively. The greenhouse bulk curing and drying system was able to cut fuel consumption by 32% compared with the conventional bulk curing barn. We could obtain almost same utilization efficiency of solar energy in 1982 compare with normal year, mainly increasing the heat receiving area.

• 한국연초학회지
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• 제31권2호
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• pp.95-106
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• 2009

Semiquantitative analysis by ICP-MS has proven to be a powerful tool for fast screening, in addition, it does not require the element of interest to be present in the calibration standard, making it especially useful for the analysis of unknown samples. In this study, seven cigarette samples were analyzed by the rapid semi-quantitative analysis method based on the ICP-MS. For each cigarette sample, cut tobacco, cigarette paper, filter (before and after smoking), and smoke condensate were analyzed. The accuracy of the analysis technique was evaluated by comparing results obtained from Calibration Check Standard(CCS) and calibration method. Relative Percentage Error(RPE) value of all elements measured for three CCS showed a stable result of less than ${\pm}20%$. Compared to full quantitative analysis by calibration method, the results for cigarette samples showed average error within ${\pm}15%$.

• 한국연초학회지
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• 제1권2호
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• pp.127-137
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• 1979

담배의 중량변화에 영향을 주는 인자중에서 刻草(각폭 0.9mm)의 길이와 수분함량 그리고 이 두 인자의 변화에 따른 팽숭성 변화의 상호관계로부터 우리나라 제조담배의 중량변화관계를 분석하였다. 1. 수분함량이 1%씩 변화할 때 각초의 팽숭성은 3.2~7.9%가 변화하였고, 제품의 중량은 5.7~7.6%의 변화를 보였으며, 이 변화율은 각초의 길이가 클수록 크게 나타나고 길이가 작을수록 작게 나타났다. 2. 일정한 수분함량( 12% )에서 각초의 길이변화에 따른 팽숭성의 변화는 3.8~22.8%로 나타났고, 제품의 중량은 2.9~29.4%의 변화를 보였으며, 이 변화폭은 수분함량이 감소할수록 커지고 증가할수록 작아지는 경향을 나타내었다. 3. 길이가 서로 다른 각초를 일정량씩 임의로 배합하였을 때의 제품중량의 변화는 각초의 길이가 2mm이상은 3.5%가 증가하는 반면 2mm이하는 3.5%가 감소하였으면 이 변화는 배합률이 25~30%에서 가장 적게 나타났다. 4. 우리나라 제조담배의 표준중량은 본 실험의 결과 분석된 적정중량에 비해서 1~9%가 높은 것으로 나타났다.

• 한국연초학회지
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• 제29권2호
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• pp.140-145
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• 2007

Acrylamide has been found in many foods. Acrylamide in foodstuffs were analyzed by a GC/MS after bromination of acrylamide or by a LC/MS for underivatized acylamide. Time consuming and laborious clean up procedures is applied for the purification of the extract, in these methods. In this study, a simple and fast method without clean up step for the analysis of acrylamide in mainstream cigarette smoke was developed by using liquid chromatography-tandem mass spectrometry (LC/MS/MS) and the effects of tobacco leaf constituents on acrylamide content was observed. The analysis of acrylamide in mainstream cigarette smoke started to collect TPM (total particulate matter) from smoking and to extract by 0.1 % acetic acid solution and then to detect by liquid chromatography tandem mass spectrometry using electrospray in the positive mode. The recovery of acrylamide in 2R4F reference cigarette was 98 % and the reproducibility was 2.5 % and the limit of detection was 1.6 ng/mL. Reducing sugars and amino acids are considered to be main precursors of acrylamide in foodstuffs. Cut tobacco contain substantial amounts of reducing sugars and amino acid which may be explained the occurrence of acrylamide in mainstream cigarette smoke. The effects of reducing sugars and total nitrogen studied in an experiment with a various tobacco types. This result indicated that reducing sugars are not limiting factor for acrylamide formation, but the level of acrylamide in cigarette smoke was significantly correlated with the total nitrogen contents.