• Title/Summary/Keyword: cut grafting

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Chemical Modification of Carbon Nanotubes and Preparation of Polystyrene/Carbon Nanotubes Composites

  • Ham, Hyeong-Taek;Koo, Chong-Min;Kim, Sang-Ouk;Park, Yeong-Suk;Chung, In-Jae
    • Macromolecular Research
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    • v.12 no.4
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    • pp.384-390
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    • 2004
  • Single-walled carbon nanotubes (SWNTs) have been chemically modified through the formation of carboxylic acid functionalities or by grafting octadecylamine and polystyrene onto them. We purified SWNTs with nitric acid to remove some remaining catalysts and amorphous carbon materials. After purification, we broke the carbon nanotubes and shortened their lengths by using a 3:1 mixture of concentrated sulfuric acid and nitric acid. During these purification and cutting processes, carboxylic acid units formed at the open ends of the SWNTs. Octa-decylamine and amino-terminated polystyrene were grafted onto the cut SWNTs by condensation reactions between the amine and carboxylic acid units. The cut SWNTs did not disperse in organic solvents, but the octadecylamine-grafted and polystyrene-grafted SWNTs dispersed well in dichloromethane and aromatic solvents (e.g., benzene, toluene). Composites were prepared by mixing polystyrene with the octadecylamine-grafted or polystyrene-grafted SWNTs. Each composite had a higher dynamic storage modulus than that of a pristine polystyrene. The composites exhibited enhanced storage moduli, complex viscosities, and unusual non-terminal behavior when compared with a monodisperse polystyrene matrix because of the good dispersion of carbon nanotubes in the polystyrene matrix.

A Study on Performance Improvement of Fruit Vegetables Automatic Grafting System (과채류 접목시스템 개선 연구)

  • Kang, Dong Hyeon;Lee, Si Young;Kim, Jong Koo;Park, Min Jung;Son, Jin Kwan;Yun, Sung-Wook;An, Se Woong;Jung, In Kyu
    • Journal of Bio-Environment Control
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    • v.26 no.3
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    • pp.215-220
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    • 2017
  • This study was conducted to improve the insufficiency of fruit vegetable grafting system developed by National Institute of Agricultural Sciences, Rural Development Administration. When the rotary blade cut the stem of scions and rootstocks, the grafting failure at curved cutting surfaces happened. The cutting depth of a tomato seedling by a rotated cutter was calculated 0.11 mm even when the cutting arm length and the maximum stem diameter were 50 mm and 5 mm, respectively. Mathematical analysis and high-speed photography showed that there was no problem by cutting in straight the stem of scions and rootstocks. The compression test of seedling stems to design the optimal shape of gripper showed that stems were not completely restored when they were compressed above 0.8 mm and 0.6 mm in case of rootstocks and scion, respectively. This study found that the bending angle of stem of tomato seedlings at the grafting period was 10 degree on average. The optimal gripper finger was the edge finger type which could be precisely set center point by adjusting the distance between fingers. In addition, it was found that most of seedling could be grasped without damage when the finger-to-finger distances is set to 2.5 mm for scion and 3.0 mm for rootstocks and finger are coated by 1 mm-thick flexible material.

Breeding of a Pink Rose Cultivar 'Noble Eye' with Few Prickles for Cut Flower (가시가 적은 분홍색 장미 '노블아이' 육성)

  • Gi, Gwang-Yeon;Hwang, In-Taek;Cho, Kyung-Chul;Yun, Bong-Ki;Choi, Kyong-Ju;Lee, Jae-Sin;Han, Tae-Ho
    • FLOWER RESEARCH JOURNAL
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    • v.18 no.4
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    • pp.299-302
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    • 2010
  • A new standard pink rose cultivar 'Noble Eye' was bred from the cross between pink standard cultivar 'Noblesse' and pink standard cultivar 'Pretty Woman' at the Jeollanamdo Agricultural Research and Extension Services (JARES). The cross was made in 2003 and, 'Noble Eye' was finally selected in 2009 after investigating characteristics for three times from 2006 to 2009. 'Noble Eye' a pink standard cultivar has good flower shape with few prickles. The major characteristics of this cultivar was $125.7stems/m^2/year$ in yield. 69.8 cm in length of cut flower, 6.6 cm in flower diameter, 40.5 in petal number, and 9.4 days in vase life. This cultivar can be propagated by both cutting and grafting. The consumer's preference of this cultivar was relatively higher than that of control cultivar, 'Noblesse'.

Rooting and Seedling Growth of Hot Pepper (Capsicum annuum L.) Cuttings as Affected by Rootone Treatment and Pinching (적심 여부와 발근촉진제 처리가 고추묘의 발근 및 생장에 미치는 영향)

  • Lee, Hee-Ju;Lee, Sang-Gyu;Lee, Jung-Myung
    • Journal of Bio-Environment Control
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    • v.18 no.4
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    • pp.393-400
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    • 2009
  • In order to find out the feasibility of producing grafted red pepper (Capsicum annuum L.) seedlings with root-removed rootstocks, rooting response and subsequent seedling growth was also evaluated. Pinching the top of pepper seedlings for cutting significantly reduced the rooting of cuttings in 10 commercial hot pepper cultivars. Normal rooting was obtained from all commercial cultivars even though the rooting response varied considerably among cultivars. Poor rooting was observed in 'Gukbo' and 'Chungyang'. Rootone treatment significantly promoted rooting in all tested cultivars and the decrease of rooting response caused by pinching could be fully recovered by rootone treatment. Among the ten commercial rootstocks developed for hot pepper grafting, 'Tantan' showed the best rooting response followed by 'Konesian Hot' and 'Wonkwang 1'. Most of these rootstocks, however, showed higher rooting response as compared to the popular commercial cultivars, 'Manit', 'Chungyang', and 'Nokkwang'. Pasting of IBA at cut surface promoted the rooting of pepper cuttings, but the effect was not quite pronounced as compared to rootone treatment.

Easy Reproducible Tie-over Dressing using Silastic$^{(R)}$ Drainage (실라스틱(Silastic$^{(R)}$) 배액관을 이용한 간편한 봉합고정 드레싱)

  • Kim, Peter Chan Woo;Park, Sang-Soon;Lee, Yong-Jig;Shim, Jeong-Su;Park, Dae-Hwan
    • Archives of Plastic Surgery
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    • v.37 no.3
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    • pp.313-316
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    • 2010
  • Purpose: Tie-over dressing is widely used to secure skin grafting on face, body, or extremities. It can be a rather complicated task and is not easy to make compressive dressing again if performed in a conventional method. So, we hereby introduce an easy reproducible tie over dressing method. Methods: After completing the skin graft, Cut the silastic drainage longitudinally in half and spread to the grafted skin margin. Drainage is fixed by using the stapes or sutures. A fluffy gauze bolus dressing is placed over a furacin impregnated gauze and wrapped around. After suturing the distal margin of silastics with opposite side using the silk thread either 5 - 0 or 3 - 0, knot of suturing, which is pressed down against the dressing while the threads are tightened, is made into center of each sides. Results: It can make dressing again after observing the grafted skin, and it can also make pressure on the grafted area evenly until the grafted skin is taken. Conclusion: This dressing method makes the surgeons and patients comfortable. To surgeons, it provides more rapid and easier way to do dressing, and to patients, it eliminates pain caused by redressing.

Evapotranspiration Rate of Grafted Seedlings Affected by Relative Humidity and Photosynthetic Photon Flux under Artificial Lighting (인공광하에서 접목묘의 증발산속도에 미치는 상대습도와 광합성유효광량자속의 효과)

  • 김용현;박현수
    • Journal of Biosystems Engineering
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    • v.26 no.4
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    • pp.379-384
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    • 2001
  • To enhance the smooth graft-taking of grafted seedlings, the excessive evapotranspiration from grafted seedlings just after grafting should be avoided. A measurement system of the evapotranspiration rate of grafted seedling under artificial lighting was set up to investigate the effect of physical environment on the evapotranspiration and graft-taking characteristics of grafted seedlings quantitatively. The evapotranspiration rate of grafted seedlings affected by relative humidity and light intensity were analyzed using the measurement system. The hypocotyl of watermelons (Citrullus vulgaris cv. Sweetdew, Hungnong Seed Co.) was slantly cut and then inserted into a hole on the stem of rootstock (Lagenaria siceraria cv. FR-King, Hungnong Seed Co.). Grafted seedlings were healed and joined for 5 days under cool-white fluorescent lamps (FL20SEX-d/18, Keumho Electric Co.) with photoperiod of 12h$.$d$\^$-1/ except dark period for one day after grafting in a closed graft-taking enhancement system developed by Kim(2000). The evapotranspiration rate and graft-taking of grafted seedling at air temperature of 23$\^{C}$ and air current speed of 0.1m$.$s$\^$-1/ was highly affected by relative humidity. But light intensity showed higher effect on the stem length of scion than relative humidity. In conclusion, it was suggested that relative humidity should be controlled at higher than 90% with photosynthetic photon flux of 50$\mu$mol$.$m$^2$$.$s$\^$-1/ to increase the survival of grafted seedlings and to produce healthy seedlings.

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Guided Tissue Regeneration Using Barrier Membrane and Osseous Grafts in Surgically Created Furcation Defects in Dogs (성견의 외과적 치근이개부 골결손에 차폐막과 골이식재를 이용한 조직유도재생술시 치유양상)

  • Chung, Eun-Hee;Chung, Hyun-Ju
    • Journal of Periodontal and Implant Science
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    • v.26 no.4
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    • pp.967-987
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    • 1996
  • The present study was to evaluate the healing patterns of guided tissue regeneration( GTR) using resorbable $Vicryl^{(R)}$(polyglactin 910) mesh and nonresorbable expanded polytetrafluoroethylene(ePTFE) membrane with or without bone grafting using autogeneous bone and demineralized freeze-dried bone allograft(DFDBA) in the grade II furcation defects. Mucoperiosteal flaps were reflected buccally in the mandibular 2nd, 3rd and 4th premolar areas and furcation defects were created surgically by removing $5{\times}6mm$ alveolar bone in 4 dogs. Root surfaces were thoroughly debrided of periodontal ligament and cementum, and notches were placed on root surface at the most apical bone level. In the right and left mandibular quadrant, each tooth was received $Vicryl^{(R)}$ mesh(ACE Surgical Supply Co., USA) only, $Vicryl^{(R)}$ mesh with DFDBA, $Vicryl^{(R)}$ mesh with autogeneous bone grafts, ePTFE membrane($Core-tex^{(R)}$ membrane, W.L. Gore & Associates Inc., USA) only, ePTFE membrane with DFDBA or ePTFE membrane with autogeneous bone grafts. For the fluorescent microscopic examination, fluorescent agents were injected at 2, 4 and 8 weeks after surgery. Four weeks after surgery, 2 dogs were sacrificed and ePTFE membranes were removed from remaining 2 dogs, which were sacrificed at 12 weeks after surgery. Undecalcified tissues were embedded in methylmethacrylate and $10{\mu}m$ thick sections were cut in a buccolingual direction. These sections were stained with hematoxylin-eosin stain and Masson's trichrome stain, and evaluated by descriptive histology and linear measurements. The results were as follows : 1) $Vicryl^{(R)}$ mesh group showed less connective tissue attachment than ePTFE membrane group. 2) The combination of GTR using $Vicryl^{(R)}$ mesh and osseous grafts resulted in new attachment and new bone formation more than GTR using $Vicryl^{(R)}$ mesh only. 3) GTR using ePTFE membrane, with or without osseous grafts, enhanced periodontal regeneration. 4) Root resorption and dentoalveolar ankylosis were observed in the areas treated with the combination of GTR and DFDBA. It was suggested that the effect of adjunctive bone grafting in GTR procedure depends on the materials and the physical properties of barrier membranes. $Vicryl^{(R)}$ mesh performed a barrier function and the use of adjunctive bone grafting may enhance the periodontal regeneration.

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Rat Peripheral Nerve Regeneration Using Nerve Guidance Channel by Porcine Small Intestinal Submucosa

  • Yi, Jin-Seok;Lee, Hyung-Jin;Lee, Hong-Jae;Lee, Il-Woo;Yang, Ji-Ho
    • Journal of Korean Neurosurgical Society
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    • v.53 no.2
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    • pp.65-71
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    • 2013
  • Objective : In order to develop a novel nerve guidance channel using porcine small intestinal submucosa (SIS) for nerve regeneration, we investigated the possibility of SIS, a tissue consisting of acellular collagen material without cellular immunogenicity, and containing many kinds of growth factors, as a natural material with a new bioactive functionality. Methods : Left sciatic nerves were cut 5 mm in length, in 14 Sprague-Dawley rats. Grafts between the cut nerve ends were performed with a silicone tube (Silicon group, n=7) and rolled porcine SIS (SIS group, n=7). All rats underwent a motor function test and an electromyography (EMG) study on 4 and 10 weeks after grafting. After last EMG studies, the grafts, including proximal and distal nerve segments, were retrieved for histological analysis. Results : Foot ulcers, due to hypesthesia, were fewer in SIS group than in Silicon group. The run time tests for motor function study were 2.67 seconds in Silicon group and 5.92 seconds in SIS group. Rats in SIS group showed a better EMG response for distal motor latency and amplitude than in Silicon group. Histologically, all grafts contained some axons and myelination. However, the number of axons and the degree of myelination were significantly higher in SIS group than Silicon group. Conclusion : These results show that the porcine SIS was an excellent option as a natural biomaterial for peripheral nerve regeneration since this material contains many kinds of nerve growth factors. Furthermore, it could be used as a biocompatible barrier covering neural tissue.

Breeding of Orange Rose "Peach Pudding" with Good Color and Shape (화형, 화색이 우수한 오렌지색 절화용 스탠다드 장미 "피치푸딩" 육성)

  • Kim, Won-Hee;Kim, Seung-Tae;Huh, Kun-Yang;Lee, Eun-Kyung;Park, Pil-Man;Kim, Young-Jin
    • Korean Journal of Breeding Science
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    • v.41 no.1
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    • pp.40-43
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    • 2009
  • A new standard rose cultivar "Peach Pudding" was bred from the cross between deep red standard cultivar 'Red Sandra' and light pink standard cultivar 'Dolores' at the National Horticulture Research Institute. The cross was made in 2000 and "Peach Pudding" was finally selected in 2006 after investigating characteristics for three years from 2002 to 2006. 'Peach Pudding', a pink standard cultivar has good flower shape and fashionable flower color. The major characteristics of this cultivar are 113.1 stems/$m^2$/year in yield, 60.6 cm in length of cut flower, 8.7 cm in flower diameter, 38.6 in petal number, and 12.8 days in vase life. This cultivar can be propagated by both cutting and grafting. The consumer's preference of this cultivar is relatively higher than that of control cultivar, 'New Miracle'.

Breeding of Pink Rose 'Happy Day' with Powdery Mildew (Sphaerotheca pannosa var. rosae) Resistant (흰가루병에 강한 분홍색 장미 'Happy Day' 육성)

  • Kim, Seung-Tae;Kim, Won-Hee;Kim, Young-Jin;Huh, Kun-Yang;Lee, Eun-Kyung;Park, Pil-Man
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.3
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    • pp.190-193
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    • 2009
  • A new standard rose cultivar 'Happy Day' was bred from the cross between pink standard cultivar 'Vivaldi' and pink standard cultivar 'Medeo' at the National Institute of Horticulture & Herbal Science. The first cross was in 2003 and 'Happy Day' was finally selected in 2008 after investigating characteristics for three years from 2006 to 2008. 'Happy Day', a pink standard cultivar grows vigorously and has powder mildew resistance. The major characteristics of this cultivar are $160stems\;m^{-2}$ per year in yield, 79.2 cm in length of cut flower, 9.9 cm in flower diameter, 28.8 in mean petal number, and 11.8 days in vase life. This cultivar can be propagated by both cutting and grafting. The consumers' preference of this cultivar is relatively higher than that of control cultivar, 'Nobleless'.