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Current status on the occurrence and management of disease, insect and mite pests in the non-chemical or organic apple orchards (무농약 유기재배 사과원의 병해충 발생 및 관리 실태)

  • Choi, Kyung-Hee;Lee, Dong-Hyuk;Song, Yang-Yik;Nam, Jong-Chul;Lee, Soon-Won
    • Proceedings of the Korean Society of Organic Agriculture Conference
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    • 2009.12a
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    • pp.45-56
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    • 2009
  • Current status on the occurrence and the management of the major disease, insect and mite pests were investigated in the organic or non-chemical pest control orchards from 2005 to 2009. Numbers of certified organic or non-chemical apple orchards were increased from 14 in 2005 to 78 in 2008. Severe damages on leaves and fruits occurred by the several diseases such as marssonina blotch, bitter rot, white rot, sooty blotch and flyspeck, and the several insect pests such as apple leaf-curling aphid, woolly apple aphid, oriental fruit moth and peach fruit moth on the almost certified organic or non-chemical pest control orchards. About 10 and 18 environmental-friendly materials were used to control diseases and insect or mite pests respectively. But, lime sulfur and bordeaux mixture to diseases and machine oil, plant oil mixed with egg yolk, and pheromone mating disruptions to insect pests were effective to control under the adequate conditions. At present, it is extremely difficult to produce organic apples in Korea. Growers must consider about and solve so many conditions on the cultivar, weather, local site, marketing and so on, before when they decide to change from conventional or IPM(Integrated Pest Management) to organic or non-chemical pest control orchards.

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Symptom and Resistance of Cultivated and Wild Capsicum Accessions to Tomato Spotted Wilt Virus (토마토반점위조바이러스에 대한 재배 및 야생형 고추 수집종의 병징과 저항성 조사)

  • Han, Jung-Heon;Lee, Won-Phil;Lee, Jun-Dae;Kim, Mi-Kyung;Choi, Hong-Soo;Yoon, Jae-Bok
    • Research in Plant Disease
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    • v.17 no.1
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    • pp.59-65
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    • 2011
  • One hundred Capsicum accessions were screened for symptomatic response and resistance to Tomato spotted wilt virus-pb1 (TSWV-pb1). Symptom and its severity rating were checked by visual observation at 9, 12, 14, and 45 days after inoculation, respectively. Enzyme-linked immune-sorbent assay was performed all tested individuals on non-inoculated upper leaves after the third rating to indentify viral infection. Leaf curling was predominant in almost susceptible individuals of each accession. Stem necrosis was most frequent in wild species while yellowing in commercial hybrids and Korean land race cultivars. Ring spot, a typical symptom of TSWV, was rarely detected in some of a few accessions. Different levels of resistance to TSWV-pb1 were observed among the tested accessions. High level of resistance was detected in 4 commercial cultivars of Kpc-35, -36, -57, and -62, and 8 wild species of PBI-11, C00105, PBC076, PBC280, PBC426, PBC495, PBC537, and PI201238 through seedling test by mechanical inoculation.

Identification of Bean Common Mosaic Virus Obtained from Seeds of Phaseolus vulgaris (강낭콩에서 종자전염된 Bean Common Mosaic Virus의 분류동정에 관한 연구)

  • Choi Y.M.;Lee S.H.;Park J.S.;Kim J.S.
    • Korean journal of applied entomology
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    • v.23 no.1 s.58
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    • pp.15-21
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    • 1984
  • The virus infecting French bean (Phaseolus vulgaris L.) was identified as Bean Common Mosaic Virus(BCMV) based on the host range, symptomatology, serology, morphology of virus particles and inclusion bodies. Isolates of BCMV were obtained from seeds of P. vulgaris collected at Suweon, Jangsu and Jinju in Korea. French bean produced vein clearing, mosaic, stunting and leaf curling. Symptom of Chenopodium quinoa was local lesions on the inoculated leaves, not on the upper leaves. The electron micrograph of the virus from French bean was flexuous approximately 750nm in length. Cylindrical and pinwheel cytoplasmic inclusion bodies were observed in French bean leaf infected by BCMV. BCMV from the French bean was transmitted through seed and green peach aphid, Myzus persicae. The thermal inactivation point was $55\~60^{\circ}C$, dilution end point was $10^{-3}\~10^{-5}$ and longevity in vitro was $2\~3$ days for BCMV from French bean. The isolates of BCMV reacted positively against BCMV antiserum. The extract of BCMV infected bean leaves, Azukibean mosaic virus (AZMV) and Cowpea aphid borne mosaic virus(CaMV) also reacted with BCMV antiserum, however, BCMV and CaMV showed the spur in agar gel diffusion test.

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Deformation and Stress Distribution of Discontinuous Precast Concrete Track Slab : I. Initial and Temperature Deformation (불연속 프리캐스트 콘크리트궤도 슬래브의 변형과 응력 분포 : I. 초기 및 온도 변형)

  • Lee, Dong Hoon;Kim, Ki Hyun;Jang, Seung Yup;Zi, Goangseup
    • Journal of the Korean Society for Railway
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    • v.20 no.5
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    • pp.625-636
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    • 2017
  • This study looked into the behavior of precast concrete track due to temperature variation and initial track deformation and examined the effect of initial deformation and deformation caused by temperature gradient on the stress distribution of slab under train load. In this paper, one of two papers in a series, a finite element analysis model for calculating deformation and stress of precast concrete track was proposed; the temperature distribution and displacements measured at the precast concrete track in the field were compared with the analytical results. The results show that the slab always curled up due to initial deformation; by comparing the measured displacements with the displacements calculated by taking measured temperatures at each depth as input, the effective built-in temperature (EBITD), the temperature difference between the top and bottom of the slab corresponding to the initial deformation, can be estimated. If EBITD is relevantly assumed, the calculated displacements correlate well with the measured ones.

Occurrence Monitoring and Population Growth of Tomato Russet Mite, Aculops lycopersici Massee (Acari: Eriophyidae) Using Green Label Sticker (시설토마토에서 녹색 라벨 스티커를 이용한 토마토녹응애의 발생예찰과 개체군 생장)

  • Choi, Yong Seok;Nam, Yun Gyu;Whang, In Su;Park, Hong Hyun;Kim, Hyeong Hwan;Park, Deok Gee
    • Korean journal of applied entomology
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    • v.51 no.4
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    • pp.405-410
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    • 2012
  • This study is conducted to develop a new method of early occurrence monitoring for Aculops lycopersici Massee (Acari: Eriophyidae), a major pest in tomato. A. lycopersici causes damage to plants such as curling of leaflet edges, followed by leaflet bronzing. As feeding continues by the mites, the plant takes on a russeted, wilted appearance, eventually culminating in leaflet desiccation and finally plant death. Fruit damages in tomato were shown when the density of mites was very high. Also, A. lycopersici caused more damage to unripe fruits than ripe fruits. The time taken to detect A. lycopersici on green label sticker was 7.0 seconds, the shortest. Blue, orange and white label stickers took 17.1, 19.8 and 12.3 seconds respectively. The permeable microscope with side illumination was useful in observing A. lycopersici on label stickers. A. lycopersici began to occur in late-April and the density of the mites increased rapidly after mid-May. The density peaked in mid to late-June, and decreased after late-June. A. lycopersici was observed on lower stems 20 days after the release of mites and observed on higher stems 60 days after. The peaked densities were observed on stems 40 and 60 days after and on leaf 80 days after. The label sticker is enough to monitor early occurrence of A. lycopersici in tomato cultivation facilities.

Modeling of Differential Shrinkage Equivalent Temperature Difference for Concrete Pavement Slabs (콘크리트 포장 슬래브 부등 건조수축 등가 온도차이의 모형화)

  • Lim, Jin-Sun;Choi, Ki-Hyo;Lee, Chang-Joon;Jeong, Jin-Hoon
    • International Journal of Highway Engineering
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    • v.11 no.4
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    • pp.59-68
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    • 2009
  • Torsional behavior of concrete pavement slabs due to temperature and moisture effects is constrained by self weight and friction etc, and causes stress as the result. The stress due to humidity variation in the slab is difficult to calculate while that due to temperature variation can easily be calculated by a commercial structural analysis program. Thus, the slab behavior can be predicted more accurately if the humidity effect is converted to equivalent temperature and is used as an input of structural analysis. In this study, a concrete pavement slab was constructed and strains of the slab due to environmental loadings were measured for long-term period. Thermal strains were subtracted from the measured strains by using thermal expansion coefficient of the concrete measured in a laboratory. Shrinkage strains, the remained strains, was supposed as additional thermal strains to calculate imaginary temperature with equivalent effect of the shrinkage by dividing the shrinkage with the thermal expansion coefficient. An existing shrinkage model was modified by considering the self weight and friction to be used in another model which can convert differential shrinkage between top and bottom of the slab to equivalent temperature difference. Addition research efforts on tensile stress reduction according to steady increase in the compressive strains are warranted for more accurate stress calculation.

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Current Status on the Occurrence and Management of Disease, Insect and Mite Pests in the Non-chemical or Organic Cultured Apple Orchards in Korea (무농약 유기재배 사과원의 병해충 발생과 관리 실태)

  • Choi, Kyung-Hee;Lee, Dong-Hyuk;Song, Yang-Yik;Nam, Jong-Chul;Lee, Soon-Won
    • Korean Journal of Organic Agriculture
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    • v.18 no.2
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    • pp.221-232
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    • 2010
  • During 2005~2009, current status on the occurrence and the management of the major disease, insect and mite pests were investigated in the non-chemical or organic cultured apple orchards in Korea. Numbers of certified organic or non-chemical apple orchards increased from 14 in 2005 to 78 in 2008. Severe damages on leaves and fruits were caused by the several diseases such as marssonina blotch, bitter rot, white rot, sooty blotch and flyspeck, and the several insect pests such as apple leaf-curling aphid, woolly apple aphid, oriental fruit moth and peach fruit moth on the almost certified organic or non-chemical pest control orchards. About 10 and 18 environmental-friendly materials were used to control diseases and insect or mite pests, respectively. But, lime sulfur and bordeaux mixture to diseases and machine oil, plant oil mixed with egg yolk, and pheromone mating disruptions to insect pests were effective under the adequate conditions.

Incidence and Visual Symptoms of Chilling Injury in Greenhouse Watermelons (저온환경이 수박이 냉해발생과 형태적 증상에 미치는 영향)

  • 권성환;전형권;최동칠;김채철
    • Journal of Bio-Environment Control
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    • v.10 no.1
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    • pp.36-41
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    • 2001
  • This experiment was carried out to investigate temperature distribution in the double layer plastic greenhouse and chilling injury to watermelons grown during a cold season. Temperatures on eastern and western sides were about 6.2% and 14.7%, respectively, lower than that of central section in a south-north oriented greenhouse. Daily mean temperature in the northern part was about 1-2$^{\circ}C$ higher than that in the southern part of the greenhouse. In terms of vertical temperature distribution inside the greenhouse, temperature at ground surface was approximately 1$^{\circ}C$ lower during the day and 0.5$^{\circ}C$ higher during the night than that in the upper part, 2m from the ground surface. Leaf mould medium kept higher ground temperatures as compared to sandy soil, red clay soil, and in the northern and southern sides as compared to the central part of the greenhouse. A symptom of chilling injury on leaves was upward curling, followed by chlorosis and necrosis. A severe symptom of chilling injury to plants was the breakdown of vascular bundles. Root growth was more susceptible than stem or leaf growth to low temperatures. At 3$0^{\circ}C$, main and lateral roots grew vigorously, while lateral root growth was inhibited at 22$^{\circ}C$ and root growth was stopped at 14$^{\circ}C$ and 6$^{\circ}C$. Small and puffy fruits with dark green surface were produced at low temperatures. In cold season cultivation of watermelons, it is suggested that plants be transplanted in the central part and train to sides of the greenhouse in order to reduced chilling injuries.

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Occurrence of Viral Diseases in Barley Fields and Responses of Resistant Genes to BaYMV-Ik and BaMMV (맥류 바이러스병 발생 현황 및 BaYMV-Ik와 BnMMV에 대한 저항성 유전자의 반응)

  • Park Jong-Chul;Seo Jae-Hwan;Kim Yang-Kil;Kim Jung-Gon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.3
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    • pp.197-204
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    • 2005
  • The major symptom such as yellowish and mosaic spots in overwintering barley were mostly caused by viruses such as Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV) in the nation-wide for four years. The result showed that more than $78\%$ collected samples were infected by the viruses. The incidence of Ba YMV was more than $70\%$, and relatively uniformly distributed in the southern regions of barley fields in Korea. However the incidence of BaYMV in Gyeonggi Province was as low as $19\%$ compared to $65\~85\%$ in the rest of regions. Occurrence of BaMMV varied depending on investigated regions such as $20\~40\%$ in Jeonbuk, Jeonnam, Gangwon and Gyeongnam, and a lower infection in Gyeongbuk, Chungnam and Gyeonggi Provinces. In this result, $60\%$ of BaMMV was found to be in the southwest regions of Korea such as Jeonbuk and Jeonnam Provinces. Over all, both BaYMV and BaMMV were thought to be dominantly casual agents in overwintering barley by either solely or mixed infections. Soil-borne wheat mosaic virus(SBWMV) occurred at most $14\%$ in Gyeonggi and Barley yellow dwarf virus-MAY (BYDV­MAV) was found only one place in Jeonbuk, suggesting that SBWMV and BYDV-MAV were not significant diseases in Korea. Exotic genetic resources that possess different resistant genes to BaYMV and BaMMV were tested to identify the responses to the viruses occurred in Iksan. According to the ELISA results, BaYMV and BaMMV were infected in some plant materials but SBWMV was not identified. Any resistant gene was not effective to BaYMV-Ik (Insan strain) and BaMMY. Ishukushirazu (rym 3) and Chosen (rym 3), Tokushima Mochi Hadaka (rym 4y) and Hakei 1-41 (rym 5a) showed resistant response with little symptoms to BaYMY. The other five accessions possessing rym 1+5, rym 2, rym 4m, rym 5 and rym 9, respectively, were resistant to BaMMV. Various symptoms were observed in the tested plant materials such as not only yellowish and mosaic symptoms mostly but also necrotic spot, tissue necrosis, leaf stripe and leaf curling. However, it was difficult to find any relationship between resistant genes and specific symptoms.

Detection of Lily symptomless virus, Lily mottle virus, and Cucumber mosaic virus from Lilium Grown in Korea by RT-PCR (RT-PCR법을 이용한 백합 바이러스 LSV, LMoV, CMV의 검출)

  • Lim, Ji-Hyun;Bae, Eun-Hye;Lee, Yong-Jin;Park, Sung-Han;Lee, Kyu-Jun;Kim, Sae-Ro-Mi;Jung, Yong-Tae
    • Korean Journal of Microbiology
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    • v.45 no.3
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    • pp.251-256
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    • 2009
  • Leaf samples and bulbs showing characteristic symptoms of virus infection were collected from Gang-won, Chung-nam, and Jeju Province of Korea in 2008-2009. Three viruses, Lily symptomless virus (LSV), Lily mottle virus (LMoV), and Cucumber mosaic virus (CMV) were detected by RT-PCR. Virus-infected plant samples were identified; 12 plants with LSV, 20 plants with LMoV, and 1 plant with CMV. Of the twelve LSV infected samples, seven samples were found to be mix-infected with LMoV and LSV. Symptoms of LMoV and LSV mixed infection were fairly severe, like as vein clearing, leaf curling, leaf mottling, leaf mosaic, and yellow streaking. Mixed infection with LMoV and LSV was also found in lily bulbs which have been stored under unfavorable environmental conditions. LMoV predominated in our tests, whereas spread of Lilyvirus X (LVX) was not found. The nucleotide sequences of coat protein (CP) region of seven isolates (4 LMoV, 2 LSV, and 1 CMV) were compared with the corresponding regions of LMoV (AJ564636), LSV (AJ516059) and CMV(AJ296154). The nucleotide sequence homologies between reference viruses and seven isolates were 95-99%. Complete sequencing of seven isolates is necessary to obtain more information on the molecular characteristics of these viruses as well as to increase sensitivity and rapidity of viral detection.