An optimum dietary carbohydrate content is important for maximum fish growth. In this study, we fed Wuchang bream (Megalobrama amblycephala) with either control diet (30.42%) or high carbohydrate diet (52.92%) for 90 d. Fish were fed to apparent satiation three times daily in an aquarium with automatic temperature control and circulated water. Growth performance, plasma biochemical parameters, hepatic morphology and enzyme activities were determined. It was shown that compared to fish fed control diet, fish fed high carbohydrate diet had higher plasma triglyceride and cortisol levels for d 90, and lower alkaline phosphatase level for d 45, lower hepatic superoxide dismutase and total antioxidative capacity for d 90, higher malondialdehyde for d 45 and glycogen content for d 45 and 90 (p<0.05). Histological and transmission electron microscopy studies showed that hepatocytes of fish fed high carbohydrate diet contained large lipid droplets, causing displacement of cellular organelles to periphery of hepatocytes. The relative level of hepatic heat shock protein 70 (HSP70) mRNA of Wuchang bream fed high carbohydrate diet was significantly higher than that of fish fed the control diet for 90 d (p<0.05). These changes led to decreased specific growth rate and increased feed conversion ratio (p<0.05). Upon hypoxia challenge, fish fed high carbohydrate diet had higher cumulative mortality than those fed the control diet (p<0.05). These results suggested that high dietary carbohydrate (52.92%) was detrimental to the growth performance and health of Wuchang bream.
Journal of Korean Society of Coastal and Ocean Engineers
/
v.25
no.2
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pp.52-62
/
2013
A stochastic Markov process (MP) model has been developed for evaluating the probability of failure of the armor unit of rubble-mound breakwaters as a function of time. The mathematical MP model could have been formulated by combining the counting process or renewal process (CP/RP) on the load occurrences with the damage process (DP) on the cumulative damage events, and applied to the armor units of rubble-mound breakwaters. Transition probabilities have been estimated by Monte-Carlo simulation (MCS) technique with the definition of damage level of armor units, and very well satisfies some conditions constrained in the probabilistic and physical views. The probabilities of failure have been also compared and investigated in process of time which have been calculated according to the variations of return period and safety factor being the important variables related to design of armor units of rubble-mound breakwater. In particular, it can be quantitatively found how the prior damage levels can effect on the sequent probabilities of failure. Finally, two types of methodology have been in this study proposed to evaluate straightforwardly the repair times which are indispensable to the maintenance of armor units of rubble-mound breakwaters and shown several simulation results including the cost analyses.
The organic phosphorus compounds have been widely used as an insecticide, since toxicity of these compounds is especially drastic to the insects than to men and other mammals. The organic phosphates are rapidly hydrolized and hence have little cumulative and ecologic effects. However, due to their acute toxic effects organophosphate have recorded rather high fatalities in men and domestic animals. The organic phosphorus compounds are powerful inhibitors to the carboxylic esterase enzymes such as acetylcholinesterase and pseudocholinesterase. As a result of firm binding characteristics of phosphate radicals to the active sites of enzyme, the activities of these enzymes are inhibited by the organophosphates. The organophosphates such as diazinon is easily observed from skin, gastrointestinal tract, conjunctivas and respiratory tract, and it is converted to more toxic form during metabolism in the liver The present study was carried out in order to investigate the hepatotoxicity of diazinon by observing the changes in the ultrastructure of cytoplasmic organelles of hepatic cells in albino mice. The animals were killed at 6, 12 and 24 hours after administration of 25mg/kg diazinon. The piece of hepatic tissue obtained from each animal was ultrathinly sectioned. The specimens stained by uranyl acetate and lead citrate double contrast methods were observed with JEM model 100B electron microscope. The results obtained were as follows: 1) A prominent dilatation and sacculation of the cisternae of rough endoplasmic reticulum associated with detachment of membrane bound-ribosomes, and disaggregation of the free ribosomes were recognized. 2) The hypertrophy of the smooth endoplasmic reticulum associated with depletion of the glycogen particles was observed. 3) The atrophy of cisternae of Golgi complex was observed. 4) A large number of secondary lysosomes (autophagic vacuoles and residual bodies) were formed. Consequently it is suggested that diazinon would induce disorganization of the cytoplasmic organelles of hepatocytes in albino mice.
This experiment was carried out to examine ecological response and soybean quality as affected by environmental cultivation for producing high seed quality in domestic soybean variety. The results are as follows: Under equal cumulative temperature condition, soybean plants grown in Muju showed longer days to flowering, which was an effect of the long day-length on high latitudes, and longer duration of reproductive stage as a result of low temperature within that period. Considering apparent seed quality, 100 seed weight of soybeans grown in Muju was heavier than Miryang. Ratio of seed crack and disease-damaged seeds was lower in Muju, and these parameters decreases as planting was delayed. The protein contents did not show significant difference in terms of altitude and planting date, however, crude oil contents were higher in Miryang. An opposite trend was observed in C18:1 and C18:3. In the fatty acid composition, the proportion of C18:1 decreased as seeding date was delayed, and was higher in Miryang. Opposite observations were obtained from C18:3. The anthocyanin contents were highest on June 10 planting and higher in Muju than in Miryang. Isoflavone content was higher as seeding date was delayed and is similar accross seeding dates in Muju. As a summary, for high seed quality production the optimum planting date was June 10, and Muju was more suitable region than Miryang.
Journal of the Korean Society for Aeronautical & Space Sciences
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v.36
no.12
/
pp.1186-1192
/
2008
The reliability of gas turbine engine blades was studied. Yield strength, Young’s modulus, engine speed and gas temperature were considered as statistically independent random variables. The failure probability was calculated using five different methods. Advanced Mean Value Method was the most efficient without significant loss in accuracy. When random variables were assumed to have normal, lognormal and Weibull distributions with the same means and standard deviations, the CDF of limit state equation did not change significantly with the distribution functions of random variables. The normalized sensitivity of failure probability with respect to standard deviations of random variables was the largest with gas temperature. The effect of means and standard deviations of random variables was studied. The increase in the mean of gas temperature and the standard deviation of engine speed increased the failure probability the most significantly.
Objectives: There are at least three conceptual models for the effects of the childhood social environment on adult health: the critical period model, the social mobility model, and the cumulative risk model. However, few studies have investigated all three different models within the same setting. This study aims to examine the impact of childhood and adulthood socioeconomic positions and intergenerational social mobility over the life course on the health in adulthood based both on the critical period model and the social mobility model. Methods: This study was conducted on 9583 adults aged between 25 and 64 years old and they were the respondents to the Korea Welfare Panel Study (2006). A multivariate logistic regression analysis was carried out, using the critical period model and the social mobility model out of the life course approaches, to look into the impact of childhood and adulthood socioeconomic positions and intergenerational social mobility on the health status in adulthood. Results: Household income and occupation out of the adulthood socioeconomic position indicators had an independent influence on the adulthood health status. The childhood socioeconomic position indicators, except for the place of childhood residence, affected the adulthood health status even after adjustment for the adulthood socioeconomic position. The effect of intergenerational social mobility was also statistically significant even after adjusting for the adulthood socioeconomic position, but it became insignificant when the childhood socioeconomic position was additionally adjusted for. Conclusions: Adulthood health is indeed affected by both the childhood and adulthood socioeconomic positions as well as intergenerational social mobility. This result shows that a life course approach needs to be adopted when dealing with health issues.
Recently, the demand for pollution-free demolition work of old reinforced concrete and steel structure has rapidly increased as the redevelopment of urban area has been accelerated. This study deals with linear shape charges for explosive jet cutting on steel structure. We have tested material and shape of steel structure, characteristics of thickness and strength, shape of linear shape charges, type of shape charges, cumulative charges, type of liner, stand-off distance, detonation method. effect of sound and vibration by air blast in explosive jet cutting method. So, We developed linear shape charges in order to take advantage of optimum explosive jet cutting condition. Shape charges were made of PETN explosives. We obtained the experimental formula to decide the amount of explosive needed for thickness of steel structure plate. There are prospects for application of the explosion curving technology under the open space conditions for dismantling the steel structure and steel bridge, scrapped old boats, which are going out of service.
Kim, Hye-Jin;Song, Jin-A;Lim, You-Jin;Chung, Doug-Young
Korean Journal of Soil Science and Fertilizer
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v.45
no.1
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pp.112-117
/
2012
The Revised Universal Soil Loss Equation (RUSLE) is a revision of the Universal Soil Loss Equation (USLE). However, changes for each factor of the USLE have been made in RUSLE which can be used to compute soil loss on areas only where significant overland flow occurs. RUSLE which requires standardized methods to satisfy new data requirements estimates soil movement at a particular site by utilizing the same factorial approach employed by the USLE. The rainfall erosivity in the RUSLE expressed through the R-factor to quantify the effect of raindrop impact and to reflect the amount and rate of runoff likely is associated with the rain. Calculating the R-factor value in the RUSLE equation to predict the related soil loss may be possible to analyse the variability of rainfall erosivity with long time-series of concerned rainfall data. However, daily time step models cannot return proper estimates when run on other specific rainfall patters such as storm and daily cumulative precipitation. Therefore, it is desirable that cross-checking is carried out amongst different time-aggregations typical rainfall event may cause error in estimating the potential soil loss in definite conditions.
Kim, Chang-Gon;Choe, Hyeon-Seung;Jang, Hak-Jin;Yun, Seok-Yeong;Kim, Tae-Ok
Korean Journal of Materials Research
/
v.11
no.11
/
pp.972-977
/
2001
It was investigated the effect of ultrasonic treatment on magnetic property of ultra fine magnetic Fe powders ($\alpha$-Fe) for magnetic tape. The properties were characterized with vibrating sample magnetometer (VSM), particle size analyzer (PSA) and scanning electron microscope (SEM). At 4hours ultrasonic treatment of 70 kHz, magnetic properties such as squareness ratio (S.Q. : 0.8868), orientation ratio (O.R. : 2.45) and switching field distribution (S.F. D. : 0.394) before taping were relatively enhanced. Cumulative particle size distribution of less than 5$\mu\textrm{m}$ fine powder was above 90% below 1 hour leaving time after ultrasonic treatment and it was supposed that ultrasonic treatment prevent aggregation. The magnetic values of S.Q. and S.F.D. of tape manufactured at 70 kHz and 4hour ultrasonic treatment were improved from 0.7747, 0.3818 to 0.8037, 0.3706, respectively. Electro-magnetic property used as in-output signal characteristic was improved, which showed that ultrasonic treatment developed the magnetic properties.
Background: Peripheral nerve injury sometimes leads to chronic neuropathic pain such as causalgia. A subset of patients with causalgia have a sympathetically maintained pain which is often evoked by cooling stimuli. However, our knowledge on adrenergic receptor types responsible for cold-evoked pain that is sympathetically dependent is lacking. The present study was conducted to investigate subtypes of adrenoceptors involved in mediating cold-evoked pain that developed following peripheral nerve injury. Methods: Neuropathic surgery was performed by a unilateral ligation of L5 and L6 spinal nerves of rats. Behavioral sign of cold-evoked pain was examined for 5 min by measuring cumulative duration of time that the rat lifted its foot off a metal plate held at cold temperature ($5^{\circ}C$). Whether cold-evoked pain behavior was affected by antagonists of various subtypes of adrenoceptors, which were administered intraperitoneally before and after the ligation, was investigated. Results: After ligation, duration of foot lifting on the ligated side at cold temperature increased as compared to the pre-operative period. This increase maintained for the entire 40-day test period. Pretreatment with alpha-antagonist phentolamine produced a suppression of cold-evoked pain behavior that was not affected by beta-antagonist propranolol pretreatment. Prazosin, alpha-1 antagonist, suppressed cold- evoked pain behavior when treated either before or after nerve ligation. On the other hand, alpha-2 antagonist yohimbine was without effect on cold-evoked pain behavior whether it was treated before or after the ligation. Conclusions: The results suggest that peripheral nerve injury develops cold-evoked pain that is sympathetically dependent, and that alpha-1 adrenoreceptor plays a critical role for the generation of this type of pain in its initiation as well as maintenance.
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