• Asian-Australasian Journal of Animal Sciences
• /
• 제18권10호
• /
• pp.1414-1420
• /
• 2005

The purpose of this research was to determine whether or not feeding induced hypovolemia (decreases in plasma volume) and decreases in plasma bicarbonate concentration caused by loss of $NaHCO_3$ from the blood, act to suppress feed intake and saliva secretion volumes during the initial stages of feeding in goats fed on dry forage. The animals were fed twice a day at 10:30 and at 16:00 for 2 h each time. Prior to the morning feeding, the collected saliva (3-5 kg) was infused into the rumen. During the morning 2 h feeding period (10:30 to 12:30), the animals were fed 2-3 kg of roughly crushed alfalfa hay cubes. At 16:00, the animals were fed again with 0.8 kg of alfalfa hay cubes, 200 g of commercial ground concentrate and 20 g of sodium bicarbonate. In order to compensate for water or $NaHCO_3$ lost through saliva during initial stages of feeding, a 3 h intravenous infusion (17-19 ml/min) of artificial mixed saliva (ASI) or mannitol solution (MI) was begun 1 h prior to the morning feeding and continued until the conclusion of the 2 h feeding period. The physiological state of the goats in the present experiment remained unchanged after parotid gland fistulation. Circulating plasma volume decreases caused by feeding (estimated by increases in plasma total protein concentration) were significantly suppressed by the ASI and MI treatments. During the first 1 h of the 2 h feeding period, plasma osmolality in the ASI treatment was the same as the NI (non-infusion control) treatment, while plasma osmolality in the MI treatment was significantly higher. In comparison to the NI treatment, cumulative feed intake levels for the duration of the 2 h feeding period in the ASI and MI treatments increased markedly by 56.6 and 88.3%, respectively. On the other hand, unilateral cumulative parotid saliva secretion volume following the termination of the 2 h feeding period in the ASI treatment was 50.7% higher than that in the NI treatment. MI treatment showed the same level as the NI treatment. The results of the present experiment proved that the humoral factors involved in the suppression of feeding and saliva secretion during the initial stages of feeding in goats fed on dry forage, are feeding induced hypovolemia and decrease in plasma $HCO_3^-$ concentration caused by loss of $NaHCO_3$ from the blood.

• 한국수자원학회논문집
• /
• 제49권4호
• /
• pp.275-282
• /
• 2016

본 연구에서는 가뭄사상과 확률 가뭄심도를 결정하는 새로운 방법을 제안하였다. 강우자료로부터 가뭄사상을 추출하기 위해 연속이론과 누적 강우부족량을 동시에 고려하였다. 절단수준 이상의 강우사상이 발생할 경우, 그 때까지의 누적 강우부족량을 해갈할 수 있을 만큼의 강우량이 발생하였는가를 확인하여 가뭄사상의 종료여부를 최종 결정하였다. 이와 같이 추출된 가뭄사상의 지속기간과 심도의 상호 의존성 구조를 파악하여 결합분포함수를 추정하기 위해 코플라 함수를 적용하였다. 또한 이변량 코플라 함수의 조건부 함수를 이용하여 가뭄의 특정 지속기간에 대한 가뭄심도의 재현특성을 분석하였으며, 신뢰구간을 추정하여 이변량 빈도해석의 불확실성을 정량화하였다. 서울지점의 1909~2015년 강수자료에 적용한 결과 과거 극한가뭄으로 판단되었던 가뭄사상은 대부분 최소 10년에서 최대 50년 정도의 재현기간을 갖는 반면 2013년 발생하여 현재까지 지속되고 있는 2015년 가뭄은 현저히 높은 재현기간을 가지고 있는 것으로 나타났다. 이러한 연구 결과는 향후 가뭄대책을 마련하는 데 있어 빈도개념을 바탕으로 하는 신뢰성 있는 기준으로 활용할 수 있을 것으로 기대된다.

• Journal of Chest Surgery
• /
• 제54권2호
• /
• pp.106-116
• /
• 2021

Background: We evaluated the mid-term outcomes and angiographic patency of redo coronary artery bypass grafting (CABG). Methods: Of 2,851 patients who underwent isolated CABG at Seoul National University Hospital from 2000 to 2017, 88 underwent redo CABG. Patients' mean age at redo CABG was 66.0±8.0 years. The mean interval between the first-time and redo CABG was 113.0±62.4 months. The mean follow-up duration was 86 months. Early and mid-term clinical outcomes were evaluated. Angiographic patency rates were evaluated early (1-2 days), 1 year, and 5 years after surgery. Comparative analyses between on-pump and off-pump CABG were also performed. Results: The culprits for reoperation were previous grafts (65.6%), native coronary vessels (17.8%), and both (16.7%). Off-pump CABG was performed in 75 cases (85.2%), and the mean number of distal anastomoses was 1.8±0.8. The saphenous vein (39.7%) was used most frequently, followed by the right internal thoracic artery (28.4%), right gastroepiploic artery (21.3%), left internal thoracic artery (7.8%), and radial artery (2.8%). Operative mortality was 1.1%. The overall survival, cumulative incidence of cardiac death, and cumulative incidence of major adverse cardiac events were 71.3%,12.0%, and 23.3% at 5 years after surgery, respectively. The overall angiographic patency rates were 95.7%, 90.1%, and 92.2% on early, 1-year, and 5-year angiograms, respectively. The angiographic patency rates of saphenous vein grafts were 93.1%, 85.6%, and 91.3% on early, 1-year, and 5-year angiograms, respectively. No significant differences in clinical outcomes or angiographic patency rates were observed between the on-pump (n=13) versus off-pump (n=75) groups. Multivariable analysis revealed that age (hazard ratio [HR], 1.07; p=0.005) and chronic kidney disease (HR, 3.85; p=0.001) were risk factors for all-cause mortality. Conclusion: Redo CABG could mostly be performed using the off-pump technique and did not show increased operative mortality and morbidities.

• 한국해양학회지:바다
• /
• 제28권4호
• /
• pp.121-132
• /
• 2023

표층 수온의 이상 고수온 현상인 해양열파는 해양생태계, 수산업, 기상 현상 등 인간 활동 및 해양 생물들에게 광범위하게 영향을 미치기 때문에 많은 해양학자들의 관심을 받고 있다. 본 연구에서는 여름철 동중국해의 해양 물성 변화를 유발하는 중요한 원인인 양자강 희석수 분포가 해양열파에 미치는 영향에 대해서 살펴보았다. 동중국해 표층 염분 분포는 6~8월에 양자강 희석수의 동쪽 확장으로 인해 제주도 및 대한해협까지 희석수의 영향을 받는 것을 알 수 있었고, 9월에는 양자강 희석수가 양자강 하구역에 제한적으로 존재하는 형태를 보여주었다. 여름철 해양열파 누적강도를 Empirical Orthogonal Function (EOF) 분석을 통해 분석하였다. EOF 첫번째 모드의 고유벡터와 주성분 시계열을 추출하여 양자강 희석수와 상관관계 분석을 실시한 결과, 양자강 희석수로 인해 염분이 낮은 해역과 해양열파 누적강도가 강하게 나타나는 해역의 공간 상관성이 매우 높은 음의 상관관계를 보임을 알 수 있었다. 이는 희석수로 인한 성층 강화가 해양열파의 강도 및 지속성을 증가시킬 수 있음을 보여준다. 본 연구결과는 한반도 연근해 해양열파 발생에 있어서 대기변화와 해양 환경이 모두 중요한 요인이 될 수 있음을 시사한다.

• 벤처창업연구
• /
• 제18권6호
• /
• pp.133-145
• /
• 2023

본 연구에서는 제2벤처붐에 따른 VC의 스타트업 투자와 해당 주식에 대한 투자자들의 반응을 살펴보고자 하였다. VC는 비상장기업에 투자 후 기업가치를 제고하여 주식시장에 상장하고, 의무보유 기간 이후 매각함으로써 수익을 창출한다. 주식시장의 투자자들이 VC들의 투자방식에 적응하였는지 여부를 검증하기 위해 의무보유 기간 종료 전후 주식가격 변화에 차이가 존재함을 가정하였으며, 2017년 하반기 이후 기반이 마련된 제2벤처붐의 영향에 따른 차이를 살펴보기 위해 과거(2015년부터 2017년까지)와 최근(2020년부터 2022년까지)의 주가 영향을 비교 분석하였다. 본 연구의 주요 분석결과는 다음과 같다. 첫째, 의무보유 기간 종료에 따른 주가 변화는 과거에 종료일 전후 초과수익을 올릴 수 있었던 것과 달리 최근에는 시장보다 낮은 수익을 갖는 것으로 나타났다. 둘째, 의무보유 기간별 주가에 미치는 영향을 분석한 결과, 1개월과 12개월에서만 유의한 영향을 보여주었다. 특히, 제2벤처붐 이후 의무보유 기간이 1개월인 벤처금융 및 전문투자자의 주식은 의무보유에 대한 정보에 미리 반응하는 것을 확인할 수 있었다. 마지막으로 VC투자 여부에 따른 주가 영향에서는 제2벤처붐 이전과 달리 이후에 VC투자여부에 따라 누적초과수익률에서 차이가 존재하였다. 시사점으로는 첫째, VC들은 회수 전략을 변경할 필요성이 존재한다. VC들의 투자 및 회수 방식은 정보비대칭을 기반으로 하고 있는데, 분석결과 해당 정보로는 시장초과수익률을 달성하기 어렵기 때문이다. 둘째, 의무보유 권장기간 변경에 대한 고민이 필요하다. 상장 후 갑작스러운 주가 하락을 방지하기 위한 제도의 목적과 달리 의무보유 대상 주식은 오히려 가격 하락위험에 노출될 수 있기 때문이다. 따라서 의무보유 대상 및 권장기간을 재고하여 상장 후 투자자 보호에 대한 논의가 필요하다.

• 한국수자원학회논문집
• /
• 제49권6호
• /
• pp.565-577
• /
• 2016

본 연구에서는 인위적 수공시설물을 고려하지 않는 자연상태의 가뭄해석을 위해 자연가뭄지수(NDI)를 산정하고 활용성을 평가하였다. 자연가뭄지수는 주성분 분석을 이용하여 산정하였으며, 입력자료는 3개월 누적강수량, 자연유량, 토양수분량 자료이다. 강수량은 ASOS 59개 지점 자료이고, 자연유량 및 토양수분량은 지표수문해석모형의 결과를 이용하였다. 가뭄지수의 평가기간은 1977~2012년이며, 활용성 평가를 위해 시계열 분석, 지역별 분석 및 가뭄특성인자 분석을 수행하였다. 시계열 분석결과, 자연가뭄지수는 가뭄의 시작과 끝에 대한 평균절대오차는 0.85로 가장 정확하게 나타났다. 과거 가뭄사례와 가뭄특성인자를 분석한 결과, 자연가뭄지수의 지속기간과 발생간격의 평균절대오차는 각각 1.3, 1.0으로 가뭄의 지속기간 및 발생간격을 적절히 반영하는 것을 확인하였다. 지역별 분석결과, 자연가뭄지수는 단일변량 가뭄지수의 지역별 가뭄상황을 적절히 반영하여 활용성이 높은 것으로 나타났다. 또한, 가뭄의 시작, 끝, 지속기간, 발생간격에 대한 자연가뭄지수, 표준강수지수, 표준유출지수, 표준토양수분지수의 순위를 분석한 결과 자연가뭄지수가 가장 높은 순위로 산정되었다. 자연가뭄지수는 기존 단일변량 가뭄지수의 상이한 가뭄상황을 종합적으로 반영하며, 가뭄의 특성을 정량적으로 제시한다는 점에서 활용성이 높다고 판단된다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제13권6호
• /
• pp.761-773
• /
• 2000

Eighteen growing male Murrah buffalo (Bubalus bubalis) calves were divided into three groups consisting of six animals each and fed three urea ammoniated wheat straw (UAS) -based rations supplemented with concentrate mixtures (roughage: concentrate ratio 58:42) containing deoiled ground nut cake, GNC (8%), formaldehyde treated GNC (8%) or fish meal (8%) to undertake comparative evaluation of these rations in terms of their $CH_4$ production and growth (285 d duration) potential. A digestibility trial (10 d duration) was followed by a comparative calorimetric study in respiration chamber. Dry matter (DM) intake (84.3 to $89.3g/kg\;W^{0.75}d^{-1}$) did not differ between treatments. The digestibility coefficient of DM, organic matter (OM), crude protein (CP), neutral and acid detergent fiber did not differ significantly in different diets. Urinary energy loss as a percent of gross energy (GE) was not affected by diets. Average values of $CH_4$ production were 84.3, 77.6 and 99.1 g/d and $CH_4$ energy losses as percent of gross energy were 5.7, 5.2 and 6.1 percent on .GNC, formaldehyde treated GNC and fishmeal, respectively, and did not differ significantly. When expressed per unit of digestible OM intake, $CH_4$ production (g) was lower (p<0.05) on formaldehyde treated GNC (30.6) than on untreated GNC (30.6) and fish meal (31.9). Total ME intake and heat production were similar and hence the energy balances on different diets were similar. Nutritive value of rations in terms of digestible CP and ME were similar. Average daily gain calculated on the basis of regression of fortnights on cumulative liveweight gain in calves fed on concentrate containing unprotected GNC, protected GNC and fish meal were 437.1, 483.9 and 481.6 g, respectively. This indicated that the intake of energy was sufficient to meet the requirement of calves growing at 400 g per d. However, CP intake was around 150% of the stipulated standard (Kearl, 1982). Feed conversion ratios on unprotected GNC, protected GNC and fish meal were 11.60, 11.10 and 10.4 respectively. It was concluded that because significantly (p<0.05) low $CH_4$ is produced on protected GNC (8%), it is very good and sustainable protein source in comparison to poor quality fish meal and untreated GNC to be used in concentrate mixture for supplementing UAS-based diets.

• 대한토목학회논문집
• /
• 제40권1호
• /
• pp.25-33
• /
• 2020

가뭄사상은 지속기간, 심도, 피해면적 등으로 특성화 할 수 있다. 일반적으로 가뭄사상은 관측소별로 구축된 시계열 자료를 이용하여 가뭄지수를 산정한 후, 연속이론에 따라 가뭄의 시작과 종료 시점을 파악하여 정의된다. 하지만 이와 같은 1차원적 분석방법은 가뭄의 시공간적인 발생특성 및 이동경로를 분석하는 데에는 한계가 있다. 따라서 본 연구에서는 단순 클러스터링 알고리즘을 활용하여 3차원(경도, 위도, 시간)적 가뭄사상을 정의하고, 가뭄의 시공간적 확장에 따른 가뭄상황을 파악할 수 있는 가뭄지도를 개발하였다. 이러한 가뭄지도는 가뭄지수를 공간적으로 표출하는 2차원적 가뭄 모니터링 정보와 비교하여 3차원적 가뭄사상에 대한 특성(지속기간, 공간적 누적심도, 가뭄의 중심)을 모두 표출하는 것이다. 그 결과 가뭄 지속기간 내 가뭄 발생면적이 최소 10 % 미만인 국소면적인 경우도 있는 반면, 최대 90 % 이상으로 확장되는 비율도 44%(25개 사상 중 11개 사상)로 확인되었다. 이는 3차원적으로 해석한 다양한 가뭄 지속기간 변화에 따른 공간적인 가뭄의 면적 변화와 심도(강도) 변화에 대한 관계는 매우 중요하다는 것을 재확인 하는 것이다. 3차원 시공간적 가뭄분석을 통한 가뭄지도 개발을 위한 연구는 미래 극한가뭄 대응 방안을 마련함에 있어서는 지역적 가뭄의 시공간적 발생특성 및 패턴을 해석하는 데 활용가능성이 있을 것으로 기대된다.

• Journal of Preventive Medicine and Public Health
• /
• 제21권1호
• /
• pp.89-98
• /
• 1988

While there have been not a few reports on the seroepidemiological characteristics of hepatitis B virus (HBV) infection in Korea, most of them, however, have had several limitations; operational definition of HBV infection, validity of detection methods of HBV serologic markers, size of the study population, and confirmation of the vaccination history against HBV, etc. In order to avoid such limitations, authors randomly selected 1,495 healthy adults among the 217,511 insured (target population) of Korean Medical Insurance Corporation, living in seoul, and tested HBV serologic markers by RIA method and conducted direct interview to them. Although HBV serologic markers (HBsAg, anti-HBs and anti-HBc) of all the subjects were tested, 392(26.2%) of interview failure cases and 361 vaccinee were excluded from the actual population. Finally, the serologic markers tested of 742 nonvaccinee (study population) only were analysed for the seroepidemiologic observation of the natural infection of HBV. The seroepidemiological characteristics of HBV infection in Korea were as follows ; 1. Point prevalence of HBs antigenemia was 11.7(9.1{\sim}14.3)% in male, which was slightly higher than that of female, 9.5($3.7{\sim}15.3$)%. This level was one of the highest among those of Asian-Pacific countries. Decreasing tendency of HBsAg prevalence alter the age of 50 was observed, which seems to be due to selective attrition of HBV chronic carriers among the healthy adults and/or to the limited-lasting duration of the HBs antigenemia, in part. 2. Point prevalence of anti-HBc(78.8% in male,50.9% in female) was higher than that of anti-HBs(65.2% in male,46.6% in female), respectively. And both of them were higher in male than in female. Increasing tendency of the prevalence of both antibodies was observed by age, which seems to be largely due to recurrent infection in adults and to some cumulative effect, in part, of their relatively longer-lasting duration. 3. The level of HBV infection defined by positive for at least one of the 3 serologic markers of HBV by RIA method was 84.7($81.8{\sim}87.6$)% in male and 61.2($51.9{\sim}70.5$)% in female, which was also one of the highest among those of Asian-Pacific countries. The proportion of susceptible population to HBV infection among healthy adults was 15.3% in male and 38.8% in female. 4. The relative frequency of current or past infection and chronic carrier among HBV infected person was estimated. The currently or past infected was estimated 75.7% in male and 71.8% in female, and chronic carrier state, 13.8% in male and 14.1% in female. The analysis of the geometric mean of the antibody titer in anti-HBs positive sera indicated also to be compatible with the above findings, suggesting that active, even though inapparent, infection of HBV occur so frequently among healthy adults in Korea.

• Clinical and Experimental Reproductive Medicine
• /
• 제29권2호
• /
• pp.139-147
• /
• 2002

Objective : This study was to establish a reproducible differentiation system from the parthenogenetic mouse embryonic stem (P-mES02) cells into functional cardiomyocytes like as in vitro fertilization mouse embryonic stem (mES01) cells. Materials and Methods: To induce differentiation, P-mES02 cells were dissociated and aggregated in suspension culture environment for embryoid body (EB) formation. For differentiation into cardiomyocytes, day 4 EBs were treated with 0.75% dimethyl sulfoxide (DMSO) for another 4 days (4-/4+) and then were plated onto gelatin-coated dish. Cultured cells were observed daily using an inverted light microscope to determine the day of contraction onset and total duration of continuous contractile activity for each contracting focus. This frequency was compared with the results of DMSO not treated P-mES02 group (4-/4-) and mES01 groups (4-/4+ or 4-/4-). For confirm the generation of cardiomyocytes, beating cell masses were treated with trypsin-EDTA, dispersed cells were plated onto glass coverslips and incubated for 48 h. Attached cells were fixed using 4% paraformaldehyde and incubated with specific antibodies (Abs) to detect cardiomyocytes (anti-sarcomeric ? -actinin Ab, 1 : 100; anti-cardiac troponin I Ab, 1 : 2000) for 1 h. And the cells were finally treated with FITC or TRITC labelled 2nd Abs, respectively, then they were examined under fluorescence microscopy. Results: Rhythmically contracting areas in mES01 or P-mES02 cells were firstly appeared at 9 or 10 days after EBs plating, respectively. The highest cumulative frequency of beating EBs was not different in both treatment groups (mES01 and P-mES02, 4-/4+) with the results of 61.3 % at 13 days and 69.8% at 15 days, respectively. Also, the contracting duration of individual beating EBs was different from minimal 7 days to maximal 53 days. However, DMSO not treated groups (mES01 and P-mES02, 4-/4-) also had contracting characteristics although their frequency was a few compared to those of DMSO treated groups (6.0% and 4.0%). Cells recovered from the spontaneously contracting areas within EBs in both treated groups were stained positively with muscle specific anti-sarcomeric ? -actinin Ab and cardiac specific anti-cardiac troponin I Ab. Conclusion: This study demonstrated that the P-mES02 cell-derived cardiomyocytes displayed similarly structural properties to mES01 cell-derived cardiomyocytes and that the DMSO treatment enhanced the cardiomyocytes differentiation in vitro.