• Journal of Life Science
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• v.18 no.1
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• pp.52-57
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• 2008

Xylan is a major hemicellulose component of the cell walls of monocots and hardwood, representing up to 30% of the dry weight of these plants. To efficiently hydrolyze xylan, the endoxylanase gene from Bacillus sp. was expressed in B. subtilis DB431 by introducing the plasmid pJHKJ4. The total activity of the recombinant endoxylanase reached about 857 unit/ml by batch fermentation of B. subtilis DB431/pJHKJ4 in LB maltose medium. The majority (>92%) of endoxylanase was efficiently secreted into the culture medium. The recombinant endoxylanase hydrolyzed more the birchwood xylan efficiently than the other xylans. When 4 % concentration of xylan was used, the highest production of xylooligosaccharide was observed, and xylobiose and xylotriose were the major products. Optimal amount of enzyme and reaction time for producing xylooligosaccharide were found to be 10 unit and 1 hr, respectively. In addition, the temperature of $40^{\circ}C{\sim}50^{\circ}C$ gave the highest production of xylooligosaccharide. Consequently, the optimized conditions for the production of xylooligosaccharide through the hydrolysis of xylan were determined as follows: 10 unit endoxylanase, $50^{\circ}C$, 4% birchwood xylan, 1 hr reaction.

• Journal of Life Science
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• v.23 no.5
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• pp.676-681
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• 2013

Perchlorate (${ClO_4}^-$) is an emerging contaminant found in surface water and soil/groundwater. Microbial removal of perchlorate is the method of choice since perchlorate-reducing bacteria (PRB) can reduce perchlorate to harmless end-products. A previous study [3] showed experimental evidence of autotrophic perchlorate removal using elemental sulfur granules and activated sludge. The granular sulfur is a relatively inexpensive electron donor, and activated sludge is easily available from a wastewater treatment plant. A batch test was performed in this study to further investigate the effect of various environmental parameters on the perchlorate degradation by sludge microorganisms when elemental sulfur was used as electron donor. Results of the batch test suggest optimum conditions for autotrophic perchlorate degradation by sludge microorganisms. The results also show that sulfur-oxidizing PRB enriched from activated sludge removed perchlorate better than activated sludge. Taken together, this study suggests that autotrophic perchlorate removal using elemental sulfur and activated sludge can be improved by employing optimized environmental conditions and enrichment culture.

• Cartoon and Animation Studies
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• s.41
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• pp.153-168
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• 2015

In Kyung-GI DO, An-sung, Each local autonomous entity developed the character design to inform the public relations and revitalize local economy and culture. consequently, These have got benefits with marketing local speciality related to their an-sung character; we call it name is arum, pung-mul, duck-bugi who korea traditional face characters. On the contrary, Most of old characters which have been fallen off their formative qualities became a reason that unfit (4D,3D) special film at local doom movie theater. This thesis aims to study on the character of 'namsadang' group what have been produced in ansung-si, that the show up to people about an-sung 4D Contents what local actually character and to make it more competitive through 3D,4D spacial character project developing process. It is need for new various characters, that (3D,4D-VFX) -Graphics of new an-sung'namsadang' group. that will be total different and new development. total VFX; 4-Dimantion visual graphics released in next an-sung local festival, Business prospects are bright and receiving benefits are increasing. As seen in the case of the character of in 2015, it can be a measure to earn benefits as a higher value-added business if it is managed in the local autonomous entities. In this thesis, I propose what the of character in the local special graphics; VFX style characters design. also I was show up to '4D contents character;' ; 'namsadang' group; creative processing including planing. It is possible to be ranged from special 4D series animation, a feature-length special effct cartoon film, character products, and even local doom Theme parks show up film. which are all expected high profits. These profits will be returned back to the an-sung local autonomous entity, who can use them to develop new contents, one of foundations in local economy. As a result, it is helpful to make local traits and effective tourist attractions.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.8
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• pp.1143-1150
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• 2005

Antioxidative activities of 5 common edible seaweeds in Korea, three brown algae (seaweed fusiforme, sea mustard, sea tangle), one green algae (sea lettuce) and one red algae (laver), were examined. The antioxidative activities of ethanol extracts from these seaweeds were examined by measuring of inhibition rates against iron-induced linoleate peroxidation, DPPH (1,1-diphenyl -2-picrylhydrazyl) radical generation and MDA-BSA (malondialdehyde-bovine serum albumin) conjugation. Sea lettuce ethanol extract showed the strongest anti-oxidative activity among them, especially in inhibition against conjugation of lipid peroxide and protein. Second to sea lettuce, laver and sea tangle ethanol extracts showed high DPPH radical scavenging activity and inhibition against MDA-BSA conjugation. However, seaweed fusiforme and sea mustard ethanol extracts did not show antioxidative activities. Sea mustard contained the highest total flavonoids (11.33 mg/g dry wt) and sea lettuce contained the highest total polyphenol (8.97 mg/g dry wt) among these seaweeds. In addition, there was strong positive correlation between the antioxidative activity and total polyphenol content in these seaweeds, suggesting polyphenol compounds may contribute to antioxidative effect of seaweeds. From these data, it is suggested to consume much of seaweeds such as sea lettuce, laver and sea tangle to prevent age-related chronic diseases, and also develope neutraceutical products using polyphenol rich fraction from sea lettuce.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.7
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• pp.1084-1089
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• 2015

To develop high quality citrus products, seven lactic acid bacteria were innoculated onto ground citrus (Citrus unshiu) and cultured for 10 days. On culture days 0, 3, 5, 7, and 10, citrus ferments were withdrawn, and their antimicrobial and angiotensin-I converting enzyme (ACE) inhibitory activities were evaluated. Citrus ferments innoculated with CL-1 and CL-2, which were isolated from kimchi, showed relatively higher antimicrobial activities against food poisoning bacteria. Citrus ferments innoculated with CL-1 and CL-2 also showed stronger ACE inhibitory activities than other ferments. CL-1 and CL-2 showed more than 99% homogeny with Pediococcus acidilactici and Lactobacillus sakei, respectively, by 16S rRNA gene analysis. These results indicate that fermentation with P. acidilactici and L. sakei might contribute to the increased antimicrobial and anti-hypertensive activities of citrus.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.9
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• pp.1321-1327
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• 2011

The microorganism producing an invertase (E.C. 3.2.1.26) was isolated from wine and tentatively identified as Saccharomyces cerevisiae by cellular fatty acid analysis. The invertase was purified to homogeneity by ammonium sulfate precipitant, dialysis, ion-exchange chromatography on DEAE-Sephadex A-50, and gel chromatography on Sephadex G-200 from the culture supernatant of Saccharomyces cerevisiae JS59. The specific activity and the purification fold of the purified invertase were 7620.9 unit/mg protein and 13.9, respectively. The molecular weight of the purified invertase was estimated to be 38.5 kDa by SDS-PAGE. The optimum pH and temperature for the invertase activity were pH 5 and $55^{\circ}C$, respectively. The invertase activity was relatively stable at pH 4~6 and temperature $55^{\circ}C$. The activity of invertase was inhibited by $Ag^{2+}$ and $Hg^{2+}$, but on the contrary, activated by $Co^{2+}$ and $Mn^{2+}$. Michaelis constant ($K_m$) for invertase reaction in sucrose solution was 11.5 mM. TLC analysis of the products produced in sucrose solution during invertase reaction showed the progressive presence of glucose and fructose in accordance with sucrose hydrolysis.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.37 no.3
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• pp.219-226
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• 2011

The Echinacea, which has been commonly known as a species of composite herb of dicotyledonous plant, has been used in native American traditional medicine for the treatment of diseases like colds or other infections in North America. We artificially cultured the adventitious roots of Echinacea angustifolia using the bioreactor culture system from Echinacea angustifolia and evaluated the efficacy as a cosmetic ingredient for skin care. Several studies previously have reported neogenesis, wound healing and inflammatory inhibition effect of Echinacea angustifolia but other efficacies were not well known. In the present study, we investigated the cosmetic efficacy to know applicable value of adventitious roots cultured from Echinacea angustifolia as a cosmetic ingredient. The adventitious roots extract of Echinacea angustifolia has superior anti-oxidant effect and matrix metalloproteinase-2 inhibitory effect, compared to natural Echinacea angustifolia. These results indicate that the adventitious roots extract cultured from Echinacea angustifolia presents a new possibility of being applicable to skin care and anti-wrinkle products as a cosmetic ingredient.

• KOMUNHWA
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• no.70
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• pp.35-62
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• 2007

Songguk-ri site gained academic recognition by the excavation of stone coffin tomb with a bronze dagger in 1974. And it is confirmed that this site is epoch-making in Korean bronze age through the following excavation started in 1975. But the excavation reports published until now do not have even overall view of this site, thus it is difficult to get the whole picture of this site. Thus, in this paper the author reexamined all reports on this site and by making the map of overall view and distribution of archaeological features as a basic research. Moreover, I analyzed also artifacts from this site, and compared with the recent papers written by other researchers about the chronology and character. Songguk-ri site has livelihood domain which consists of dwelling pits, attached features, storage pits, pot-firing features, wood fence, abatises, buildings above ground, as well as cemetery which consists of stone-coffins, jar-coffins, pit tombs. Trace of making large terrace was also excavated. These features seem to belong to the same archaeological stage, dated about B.C.850-550, according to C14 dating. On the other hand, the intensification of wet-rice cultivation made this group more productive. Based on this financial strength, some influential group emerged they constructed defensive settlement to protect their products safely. Besides it seems that there were frequent occurrence of conflicts. However, we can know that they kept their stable life, through the expansion of living space. Consequently, Songguk-ri site played a role of the summit among some settlement in this area.

• KSBB Journal
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• v.19 no.2
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• pp.118-124
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• 2004

Melanocytes synthesize melanin within discrete organelle termed melanosomes which are transferred to the surrounding keratinocytes and can be produced in varying sizes, numbers and densities. Skin whitening products have become increasingly popular in the past few years. The most successful natural skin whitening agents are: Arbutin, Vitamin C, Kojic acid, Mulberry, which are all tyrosinase inhibitors. In this work, melanoston, a melanogenesis inhibitor isolated from yeast was studied to understand its mechanism of melanogenesis inhibition. It was found that melanoston was not a tyrosinase inhibitor, while when melanoston was applied to the B16 melanoma cell culture media, the intracellular tyrosinase activity was decreased by more than 30％, When B16 melanoma was stimulated with ${\alpha}$-MSH, cell morphololgy was dramatically changed to have lots of dendrites on the cell membrane surface. On the other hand, B16 was treated with ${\alpha}$-MSH and melanoston, simultaneously, the change of cell morphology was not so great. This inhibition effect of melanoston was found to be related to the inhibition of intracellular activation and transportation of tyrosinase, which was observed by immunostaining of B16 melanoma using anti-tyrosinase antibody. From these results, melanoston was regarded as an inhibitor to the differentiation of melanoma cells.

• Management & Information Systems Review
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• v.31 no.3
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• pp.47-73
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• 2012

SNS allowed users to serve not only as the content and message producer but as the consumer, with development into various types of SNS platforms. Instead of a traditional media structure of media-to-many and unspecified users, it also made it possible to achieve one-to-one or one-to-many interactions, regardless of time and space, through SNS platforms. Moreover, according to development of digital communication technology, IT, media contents and communication network have been mutually connected, though they were once separate. The changes in communication environments have caused rapid disorganization and reorganization in popular culture led by specific expert groups. Such trend has a greater influence on marketing strategies of enterprises. In other words, it will lead them to mostly introduce new information technologies, based on consumer market, and to strategically participate in SNS for promotion and marketing for their products and brands. Likewise, SNS has currently appeared as the main media affecting consumers' behaviors. In consideration of the importance of SNS features, which can stimulate responses of other users, analysis of main features affecting SNS user acceptance decision is required, as well as its utilization strategies. Accordingly, this study conducted division of SNS features into openness, quickness, interactiveness and economical efficiency to derive strategies for increasing the usage frequency of SNS and ultimately maximizing the expectation effect, in addition to an empirical analysis of effects of SNS features on usefulness, easiness and pleasure perceived in SNS, and SNS user intention.