• Title/Summary/Keyword: culture-independent

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Interrelationship between Organizational Culture, Empowerment and Trust (조직문화, 임파워먼트와 신뢰와의 관계)

  • Kim, Young-Chun;Chung, Min-Suk
    • The Journal of the Korea Contents Association
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    • v.12 no.2
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    • pp.292-300
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    • 2012
  • This study was carried out in order to verify the influence that social welfare trust organizational culture and level of empowerment. The study object 656 copies of guns were used to analysis to social worker engaged in to the community welfare agency, old man welfare agency, disabled welfare agency which was social welfare use facilities. Organizational culture and empowerment as it was for an independent variable analyzed correlation to trust and executed regression analysis to grasp a degree of an influence to be mad to trust. Research result, first, Organizational culture appeared to what I affected to trust social group culture, development cultural artist and second, Level of empowerment of social worker appeared to what I affected to trust own a task meaning result and self-determination. There's social welfare is organized in order to improve social welfare gratitude confidence, and it is group culture and development culture, and an level of empowerment to recognize a social welfare private house gives meaning anger to a task and self-determination had to heighten it.

Nitric Oxide and Embryo Development

  • Lim, Jeong M.
    • Proceedings of the KSAR Conference
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    • 2000.10a
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    • pp.5-6
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    • 2000
  • Nitric oxide (NO) is a simple combined molecule of oxygen and nitrogen, and has a wide variety of action on the physiological and pathophysiological function of the body. It is a key transducer of the vasodilator message from the endothelium to vascular cells. However, its different roles have been elucidated by numerous researches, which was undertaken in the 80's and 90's. Three types of NO synthase were involved in synthesizing NO and they are identified in different tissues and cells including macrophage, endothelial cells and even tumor cells. In the late 90's, we undertook a number of researches for elucidating the effect of NO on embryo development, since developmentally arrested bovine embryos contained large amount of NO metabolites in their cytoplasm. Subsequently, we found that the addition of a spontaneous NO donor to culture medium markedly inhibited embryo development and that its inhibitory role was independent of embryonic genome activation. Research was focused to find a way to prevent the inhibitory action of NO on embryo development and demonstrated that the addition of hemoglobin, a NO scavenger, to embryo culture medium greatly stimulated in vitro-development of bovine and mouse embryos. Based on these research outcomes, we developed a NO action-free culture system for embryos and other tissues. The efficacy of such system has subsequently been confirmed by achieving the high rates of preimplantation development and blastocyst formation in the NO action-free culture of mouse and bovine embryo. In this article, we briefly introduced the nature of NO and our research outcomes on the role of NO in embryo development.

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Medical Students' Perceptions and Intentions Regarding Patient Safety (의과대학 학생들의 환자안전에 대한 인식)

  • Lee, Hoo-Yeon;Lee, Sang-Gyu
    • Quality Improvement in Health Care
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    • v.24 no.1
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    • pp.23-29
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    • 2018
  • Background: The purpose of this study was to examine undergraduate medical students' perceptions and intentions regarding patient safety during clinical clerkships. Methods: Cross-sectional and self-administered questionnaire survey was conducted on 34 students from one medical school using a modified version of the Medical Student Safety Attitudes and Professionalism Survey (MSSAPS). We assessed $4^{rd}-year$ medical students' perceptions of the cultures ('safety', 'teamwork', and 'error disclosure'), 'behavioural intentions' concerning patient safety issues and 'overall patient safety'. The overall response rate was 66.4%. Results: Among safety domains, "teamwork culture" was rated highest. "Error disclosure culture" received the lowest ratings. Regarding the error disclosure domain, only 10% of respondents reported that they have received education or training on how to disclose medical error to patients. Independent of survey domains, when students were asked "Overall, do you think your hospital is safe based on your clinical rotation?", 61.8% reported that the hospital was safe. Conclusions: Assessing students' perceptions of safety culture can provide clerkship directors and educators with information that enhances the educational environment and promotes patient safety. Discussions of medical errors, patient safety, and how best to incorporate an analysis of these issues into the existing curriculum are needed.

Doxorubicin Productivity Improvement by the Recombinant Streptomyces peucetius with High-Copy Regulatory Genes Cultured in the Optimized Media Composition

  • PARK, HEE-SEOP;KANG, SEUNG-HOON;PARK, HYUN-JOO;KIM, EUNG-SOO
    • Journal of Microbiology and Biotechnology
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    • v.15 no.1
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    • pp.66-71
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    • 2005
  • Doxorubicin is a clinically important anticancer polyketide compound that is typically produced by Streptomyces peucetius var. caesius. To improve doxorubicin productivity by S. peucetius, a doxorubicin pathway-specific regulatory gene, dnrI, was cloned into a high-copy-number plasmid containing a catechol promoter system. The S. peucetius containing the recombinant plasmid exhibited approximately 9.5-fold higher doxorubicin productivity compared with the wild-type S. peucetius. The doxorubicin productivity by this recombinant S. peucetius strain was further improved through the optimization of culture media composition. Based on the Fractional Factorial Design (FFD), cornstarch, $K_2HPO_4$, and $MgSO_4$ were identified to be the key factors influencing doxorubicin productivity. The Response Surface Method (RSM) results based on 20 independent culture conditions with varying amounts of key factors predicted the highest theoretical doxorubicin productivity of 11.1 mg/l with corn starch of 46.33 g/l, $K_2HPO_4$ of 4.63 g/l, and $MgSO_4$ of 9.26 g/l. The doxorubicin productivity of the recombinant S. peucetius strain with the RSM-based optimized culture condition was experimentally verified to be 11.46 mg/l, which was approximately 30.8-fold higher productivity compared with the wild-type S. peucetius without culture media optimization.

Effects of Perceived Patient Safety Culture on Safety Care Activities among Nurses in General Hospitals (지방 중소병원 간호사의 환자안전문화 인식이 안전간호활동에 미치는 영향)

  • Kim, Hye Young;Lee, Eun Sook
    • Journal of East-West Nursing Research
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    • v.19 no.1
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    • pp.46-54
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    • 2013
  • Purpose: An objective of this study was to investigate nurses' perceptions toward patient safety culture and to examine the factors affecting safety care activities. Methods: The participants were 429 nurses, at 6 hospitals located in regions, which have 150 to 300 beds, and HSOPSC (AHRQ, 2009) and questionnaire on safety care activities were used as measurement tools. Descriptive statistics, independent t-test, one way ANOVA, and stepwise multiple regression with SPSS/WIN version12.0 were used to analyze the data. Results: Supervisor manager expectations and actions promoting patients safety and frequency of events reported were the highest as positive responses, whereas staffing and nonpunitive response to errors showed the lowest scores as positive responses. Scores of medication surveillance is the highest while firefighting surveillance is the lowest in terms of safety care activities. Significant predictors influencing safety care activities were frequency of events report, handoffs and transitions, work unit a patient safety grade, organizational learning-continuous improvement, and teamwork across units. These predictors account for 23% of the variance. Conclusion: These results suggest that hospital policies and systems should be built to settle patient safety culture effectively. Development of standard manuals for safety care activities is another critical element for promoting patient safety.

The Acute Toxicity Effect of Triotganotin on the Growth of Microalgae and Shellfish and A Design of A Chemostat System for the Chronic Toxicity Experiment (미세조류와 패류의 성장에 미치는 Triorganotin의 급성 독성영향 및 만성독성 실험을 위한 Chemostat System의 설계)

  • Tak, Keon-Tae;Lee, Hyong-Ho;Hong, Yoog-Ki;Kim, Joong Kyun
    • Journal of Life Science
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    • v.7 no.4
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    • pp.377-383
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    • 1997
  • The acute toxicity effect of triorganotin of trioganotin on the growth of microalgae and shellfish was investigated through flask culture. The value of 120 hr-LC$_{50}$ that is the median lethal concentration of TBTO on the shellfish (R. philipinarum) was found to be 6 $\mu$g/L. The acute toxicity effect of TBTO on T. suecica was obviously shown even at the concentration of 0.5 $\mu$g/L, and the effect diminished as the initial cell density increased. The effect also diminished less in the experiment done under aeration than in that done under non-aeration. To design a chemostat system for the test of chronic toxicity, the culture of T. suecica was executed in photobioreactor. In batch culture, the profiles of chlorophyII a and D.C.W. showed the growth of T. suecica very well, and the maximum specific growth rate was estimated to be 0.54 d$^{-1}$. with this value, as a dilution rate in contimuous culture, pH was nicely maintained between 7 and 9 when air was supplied with 3% CO$_{2}$. From all results and the natural environment of clam, a novel chemostat system was invented. Through this system, we can observe each independent toxicity effect of TBTO and plankton and combined toxicity effect as well.

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Microbiological Analysis of Dongchimi, Korean Watery Radish Kimchi, at the Early and Mid-phase Fermentation

  • Park, Sun-Jung;Chang, Jin-Hee;Cha, Seong-Kwan;Moon, Gi-Seong
    • Food Science and Biotechnology
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    • v.17 no.4
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    • pp.892-894
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    • 2008
  • During dongchimi fermentation at 5 and $25^{\circ}C$, the pH lowered slowly and reached 4.03 at $5^{\circ}C$ after 30 days, whereas it lowered dramatically and reached 3.59 at $25^{\circ}C$ after 2 days. The predominant bacteria were Leuconostoc (Leu.) mesenteroides at $25^{\circ}C$ until day 2 which changed into Lactobacillus (Lb.) plantarum at day 3, analyzed by a culture dependent method with partial 16S rRNA gene sequencing, whereas Leu. mesenteroides occupied predominantly at $5^{\circ}C$ until day 7. In a culture-independent method using a polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) with partial 16S rRNA gene sequencing, Lb. algidus was predominant at $5^{\circ}C$ until day 7 and Lb. plantarum occupied predominantly at $25^{\circ}C$ until day 3, which is different from the results of the culture based method, indicating the both methods need to be combined for accuracy. Based on the culture-dependent method, Leu. mesenteroides might be responsible for the early and mid-phase of dongchimi fermentation.

Comprehensive Study on the Origins and Changes in Kimchi Recipe (김치의 기원과 제조변천과정에 대한 종합적 연구)

  • Park, Chae-Lin
    • Journal of the Korean Society of Food Culture
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    • v.34 no.2
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    • pp.93-111
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    • 2019
  • This paper provides a comprehensive description of previous studies of making and developing kimchi in terms of the origins of kimchi and the changes in the history of kimchi, along with newly discovered data, including the collection of works in Joseon Dynasty as well as old cookbooks discovered after the year 2000, and latest research in related disciplines, e.g., ancient history studies, archeology, and linguistics. Because new ruins and relics, such as the Liao-ho Civilization in the Northeast Asia continent, which was closely related to Dongyi tribes, have been discovered and studied, it is important to determine how to reflect the outcome of archeological studies on the origin of preserved vegetables. In addition, to describe the background and changes in the independent formation of making Korean kimchi chronologically, they were divided into the following: development from jjanji to singunji; formation of the basis for seokbakji dressed with fermented fish sauce; settlement of seokbakji culture for dressing seokbakji with fermented fish sauce and spices, including chili varieties when they were introduced; and establishment of the method for making whole cabbage kimchi, which is currently typical kimchi made by adding the seokbakji as the stuffing of the cabbage kimchi, to examine the time and specific details of the change.

Impacts of the Hydraulic Loading Rate and C/N Ratio on Nitrification in a Trickling Filter with Styrofoam Bead Media in Seawater (살수식 여과조의 질산화작용에 대한 수리학적 부하량과 C/N 비의 영향)

  • Choi, TaeGun;Kim, Pyong-kih;Park, JeongHwan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.52 no.3
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    • pp.256-267
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    • 2019
  • Styrofoam beads, which are relatively inexpensive and can provide a large specific surface area, were tested as filter media. Styrofoam beads with a diameter of $3{\pm}0.5mm$ were used; the specific surface area of the beads was $1,034m^2{\cdot}m^{-3}$. Five independent recirculating culture systems were used in the experiment. Each system consisted of one culture tank and three trickling bio-filters. Using the systems, nitrification efficiency was evaluated with respect to hydraulic loading rate (HLR) and carbon/nitrogen (C/N) ratio. The lowest ammonia and nitrogen concentrations were $0.84mg{\cdot}L^{-1}$ and $1.30mg{\cdot}L^{-1}$, respectively, observed at an HLR of $50.9m^3{\cdot}m^{-2}{\cdot}h^{-1}$. Nitrification efficiency in the culture tank was highest at a C/N ratio of 0, with ammonia and nitrite nitrogen concentrations of $0.32mg{\cdot}L^{-1}$ and $0.90mg{\cdot}L^{-1}$, respectively. Ammonia and nitrite nitrogen concentrations in the culture tank abruptly changed at C/N ratios ${\geq}3$.

Personality-Culture Interaction as a Predictor of Emotion Suppression on Facebook

  • Kim, Jinhee;Stavrositu, Carmen D.
    • Science of Emotion and Sensibility
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    • v.24 no.4
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    • pp.91-106
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    • 2021
  • Although personality and culture have been employed as independent predictors of emotion regulation, less is known about the interplay between them. Thus, the present study tests their interaction by focusing on the match between personality (public self-consciousness) and culture (valuing independence vs. interdependence) in modulating an emotion regulation strategy, namely, emotion suppression, on Facebook. Furthermore, relationship concern related to the expression of positive and negative emotions on Facebook is explored as a potential underlying mechanism. An online survey on Facebook users in the United States (n = 320) and South Korea (n = 336) was conducted through two professional survey companies. The results revealed that the positive association between public self-consciousness and emotion suppression was stronger among respondents who value interdependence (vs. independence), which led to a significant interaction between the two predictors. Furthermore, public self-consciousness was associated with emotion suppression through relationship concern for the expression of positive, but not negative, emotions. Furthermore, this mediated relationship was stronger among respondents who value interdependence (vs. independence). Lastly, the study discussed the importance of exploring the interplay between personality and culture and the implication of dialectic emotions.