• Journal of Embryo Transfer
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• v.30 no.4
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• pp.315-317
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• 2015

In vitro culture of murine embryos is an important step for in vitro production systems including in vitro fertilization and generations of genetically engineered mice. M16 is widely used commercialized culture media for the murine embryos. Compared to other media such as potassium simplex optimization medium, commercial M16 (Sigma) media lacks of amino acid, glutamine and antibiotics. In the present study, we optimized M16 based embryo culture system using commercialized antibiotics-glutamine or amino acids supplements. In vivo derived murine zygote were M16 media were supplemented with commercial Penicillin-Streptomycin-Glutamine solution (PSG; Gibco) or MEM Non-Essential Amino Acids solution (NEAA; Gibco) as experimental design. Addition of PSG did not improved cleavage and blastocyst rates. On the other hand, cleavage rate is not different between control and NEAA treated group, however, blastocyst formation is significantly (P<0.05) improved in NEAA treated group. Developmental competence between PSG and NEAA treated groups were also compared. Between two groups, cleavage rate was similar. However, blastocyst formation rate is significantly improved in NEAA treated group. Taken together, beneficial effect of NEAA on murine embryos development was confirmed. Effect of antibiotics and glutamine addition to M16 media is still not clear in the study.

• Journal of Plant Biology
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• v.26 no.4
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• pp.191-196
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• 1983

The isolatin and culture of protoplasts from hypocotyl originated callus of Phaseolus vulgaris cv. Damyang were carried out. The maximum protoplast yield of 4.6$\times$105 per gram fresh callus, using the 13-day-old callus, was obtained by digeston for 6 hours in the enzyme solution. After 10 day-culture of the isolated callus protoplsts, plating efficiency was 50%. Thereafter, cell cluster medium, and followed by leading to callus formation on an agar medium after 3 weeks of the liquid culture.

• Journal of Bio-Environment Control
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• v.16 no.1
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• pp.7-12
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• 2007

This study was conducted to clarify the effects of the different concentration of the nutrient solution on the early growth and the nutritional contents of hydroponically grown Aster koraiensis, Hemerocallis fulva and Plantago asiatica at 70 days after transplanting in perlite culture. Balanced nutrient solution formulated by Japanese Horticultural Experiment Station was used as the standard concentration of the nutrient solution. Overall plant growth of Aster koraiensis and Hemerocallis fulva such as plant height, stem diameter, number of loaves, fresh and dry shoot and root weight were significantly increased in 1.5 times concentration of nutrient solution. Shoot and root fresh and dry weight of hydroponically grown Plantago asiatica were significantly increased in the higher concentration of the nutrient solution, however, number of loaves and root length were significantly increased in the standard and the lower concentration of the nutrient solution. The highest contents of calcium, magnesium and sodium in plants were shown in Aster koraienxis which were grown in the 1.5 times concentration of nutrient solution, and Hemerocallis fulva and Plantago asiatica in 0.25 times of that. The contents of potassium in Aster koraiensis and Hemerocallis fulva were significantly increased when the plants were grown in the 0.5 times concentration of the nutrient solution, and that in Plantago asiatica in the 1.5 times concentration of the nutrient solution. The contents of phosphoric acid in plants as affected by the different species of Namul and the different concentration of the nutrient solution were not significant.

• Korean Journal of Plant Tissue Culture
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• v.25 no.5
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• pp.335-339
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• 1998

The factors affecting the isolation and culture of the protoplast of embryogenic callus, derived from immature ovule in Citrus junos, were examined. An incubation time in enzyme solution of 16 hrs was preferable for protoplast isolation. Efficient protoplast yields were obtained from the treatment of equal concentration of 0.7 M $\textrm{BH}_{3}$ to the enzyme solution containing 1.0% cellulase, 1.0% macerozyme and 0.2% pectolyase. Protoplast cultured in MT medium with 0.1 mg/L 2,4-D showed vigorous division and some of them formed callus. Induced callus was subcultured on solid MT medium but the callus showed very slow growth. The above results show the possibility to culture from protoplast fusion in Citrus genera.

• Research in Plant Disease
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• v.13 no.1
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• pp.40-44
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• 2007

Powdery mildew of pepper is one of the most devastating diseases which is occurring all the year under greenhouse condition. In this study, control efficacy against powdery mildew was evaluated by mixed culture solutions of two chitinolytic bacteria, Lysobacter enzymogenenes strain C-3 and Chrornobacterium sp. strain C-61, cultivated in the chitin-supplemented medium. In all experiments, white powder on the reverse side of pepper leaves perfectly disappeared 3 days after application of mixed culture solutions. However, periods required for formation of new white powder on the same sites after application (control-lasting period) were largely differed according to environmental conditions. In particular, the control-lasting period was much longer when sprayed on 6 PM than 9 AM and especially, on rainy days than sunny days. This indicates that control efficacy of culture solution may be largely affected by environmental conditions after application. The undiluted culture solution resulted in a perfect control with control value more than 95% by application of 5-day-intervals under severely diseased field and 7-day-intervals under disease-started field. A ten-fold diluted product also showed control value more than 81% by application of the same method. These results suggest that this culture solution can be practically used to control powdery mildew disease in pepper plants.

• KSBB Journal
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• v.19 no.3
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• pp.241-244
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• 2004

An automated flow injection analysis apparatus manufactured economically was used in the automatic measurement of E. col growth in shake-flask culture of nutrient broth. The detailed measurement of whole growth was possible at intervals of 10 minutes by the automatic measurement system which adopted the sterilized nutrient broth as a carrier solution. Using distilled water as a carrier solution showed less accurate results than nutrient broth.

• Journal of Bio-Environment Control
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• v.5 no.1
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• pp.79-88
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• 1996

The purpose of this research was to develop labor-saving cultural practices of cherry tomatoes in the deep flow technique (DFT) by omitting the process of raising seedling. Three cultivars, ‘Pepe’, ‘Pico’ and ‘Koko’ were propagated by the immediate setting of cuttings(lateral shoots) to the culture bed and by setting seedlings obtained by raising cuttings for 15 days, and grown in the standard and half - strength solution. 1. The immediate setting resulted in higher plants, larger number of leaves and larger leaf area during the early growth stage. And the harvest time was hastened by six days in the direct setting than that in the seedling setting. It was also earlier in ‘Pepe’, but not significant by different between two strengths of nutrient solution. 2. The average fruit weight was affected only by cultivars, but not by the type of cutting and the strength of nutrient solution. The highest yields were obtained from the immediate setting of ‘Pepe’ in other cultivars grown in the nutrient solution of standard strength. 3. Brix degree of treated cherry tomato juice was higher in ‘Pepe’ tomato cultured by immediate setting than others.

• The Research Journal of the Costume Culture
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• v.11 no.3
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• pp.441-446
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• 2003

Water-insoluble chitosan with molecular weight of 2,000,000, 500,000, 80,000, and 40,000 and more than 90% of degree of deacetylation were used to test antibacterial activity of chitosan against a pathogenic bacteria, methicillin resistant Staphylococcus aureus(MRSA), which is being issued in the world. As experimental method, Agar plate Smear Method and Agar plate Contact Method were used. The moleculur weight of chitosan didn't exert significant influences on its antibacterial activity against MRSA but chitosan having molecular weight 40,000, 80,000 and 150,000 showed the excellent antibacterial activity. The antibacterial efHciency was excellent in applying it after chitosan was dissolved in acetic acid solution, while the antibacterial efficiency was not expressed nearly in case of applying after chitosan was dissolved in neutral water. Therefore, it is considered that chitosan can show the antibacterial efficiency only if a positive ion status of -NH₃/sup +/ is maintained. MIC of chitosan/acetic acid solution and cotton fabrics finished with chitosan/acetic acid solution showed in concentration of 0.05%.

• The Research Journal of the Costume Culture
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• v.12 no.5
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• pp.781-791
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• 2004

Currently, as mordants, heavy metals are being used in dyeing process in order to improve dye uptake and dye fastness. These chemicals, however, are mostly harmful to human body and the environment along with the pollution by effluent. Traditionally, rice straw ash solution, lime, etc. have been used as mordants in order to improve the effects on dye uptake and color fastness of dyestuffs. The study of natural mordants would, therefore, be vital to the development and succession of our traditional dyeing methods. In this study, as a preliminary step toward the use of rice straw ash solution for the nautral dyeing using Caesalpinia sappan, several aluminum compounds were employed as mordants. When the purified aluminum compounds were used, on the basis of unmordanted cotton fabric, the K/S values of the pre-mordanted and simultaneously mordanted fabrics increased by 2 or 3 times, while those of the post-mordanted fabrics decreased.

• Journal of agriculture & life science
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• v.44 no.6
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• pp.23-26
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• 2010

Commercial fibers such as two kinds of micro-fiber, flannel, and cotton were analyzed for their nutrient solution absorption capacity to select hydrophilic mat used for the irrigation management by drainage level sensor in perlite bag culture. The selected mat was evaluated in terms of absorption capacity. Cotton had the highest absorption capacity and was revealed to be the most appropriate for the control system.