• Journal of Microbiology and Biotechnology
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• v.18 no.5
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• pp.897-900
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• 2008

Various sequences of pH change were applied in a batch bioreactor to investigate pH shock effects on geldanamycin production by Streptomyces hygroscopicus subsp. duamyceticus JCM4427. In the control culture where the pH was not controlled, the maximum geldanamycin concentration was 414 mg/l. With the pHS1 mode of pH shock, that is, an abrupt pH change from pH 6.5 to pH 5.0 and then being maintained at around pH 5.0 afterward, 768 mg/l of geldanamycin was produced. With pHS2, in which the pH was changed sequentially from pH 6.7 to pH 5.0 and then back to pH 6.0, 429 mg/l of geldanamycin was produced. With pHS3 having a sequential pH change from pH 6.0 to pH 4.0 and then back to pH 6.5 followed by the third pH shock to pH 5.5, no geldanamycin production was observed. Considering that the productivity with pHS1 was about two-fold of that of the control culture with no pH control, we concluded that a more sophisticated manipulation of pH would further promote geldanamycin production.

• The Journal of the Convergence on Culture Technology
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• v.9 no.1
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• pp.463-469
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• 2023

The aim of this study is to provide basic data that can contribute to improving successful clinical adaptation and reducing turnover of new graduate nurses by analyzing research related to reality shock experienced by new graduate nurses using text network analysis. The topics of reality shock experienced by new graduate nurses were extracted from 115 papers published in domestic and foreign journals from January 2002 to December 2021. Articles were retrieved from 6 databases (Korean DB: DBpia, KISS, RISS /International DB: Web of science, Springer, Scopus). Keywords were extracted from the abstract and organized using semantic morphemes. Network analysis and topic modeling for subject knowledge structure analysis were performed using NetMiner 4.5.0 program. The core keywords included 'new graduate nurses', 'reality shock', 'transition', 'student nurse', 'experience', 'practice', 'work environment', 'role', 'care' and 'education'. In recent articles on reality shock experienced by new graduate nurses, three major topics were extracted by LDA (Latent Dirichlet Allocation) techniques: 'turnover', 'work environment', 'experience of transition'. Based on this research, the necessity of interventional research that can effectively reduce the reality shock experienced by new graduate nurses and successfully help clinical adaptation is suggested.

• The Journal of the Convergence on Culture Technology
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• v.9 no.5
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• pp.245-253
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• 2023

The purpose of this study was to investigate the effect of pre-service early childhood teachers' reality shock and school burnout on career search self-efficacy. The study participants were 195 pre-service early childhood teachers. The results of the study were as follows: First, it was found that pre-service early childhood teachers had relatively high level of reality shock, school burnout, and career search self-efficacy. Second, there was a significant positive correlation between reality shock and school burnout and negative correlation between reality shock, school burnout, and career search self-efficacy of pre-service early childhood teachers. Third, pre-service early childhood teachers' reality shock and school burnout significantly influenced career search self-efficacy. The result of this study provides educational basic data on an early childhood teacher training program in improving career search self-efficacy.

• Journal of the Korea Safety Management & Science
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• v.9 no.3
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• pp.1-7
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• 2007

A roadmap for Electrical Safety Technology has been designed based on the study that has been contributed by the specialists, professors and researchers in electral safety field. The roadmap is divided into five major fields including, for instance, the electrical fire accidents roadmap. The other four roadmaps are for electrical shock accidents, electrical facilities accidents, electrical safety policies and electrical safety culture-education, respectively. Each roadmap has a purpose, a scope, and a technical characteristic. Following the roadmaps can result in significant reduction of electrical fire accidents, electrical shock accident victims and so forth. The result can also be utilized in guiding the planning and development of the future electrical safety technology.

• Journal of Microbiology and Biotechnology
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• v.8 no.4
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• pp.361-368
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• 1998

The E2 protein of HCV (hepatitis C virus) is thought to have a potential role in the development of subunit vaccines and diagnostics. To express it by the insect cell/baculovirus expression (Bacu) system, we constructed a recombinant Autographa californica nuclear polyhedrosis virus (AcIL3E2), determined the most appropriate expression conditions in terms of host cell line and culture medium, and characterized the expressed HCV E2 protein. A culture system using Trichoplusia ni BTI-TN5Bl-4 cells and SF 900IISFM medium expressed a relatively high level of HCV E2 protein. It was revealed that its glycosylation properties and subcellular localization were almost the same as the ones in the mammalian cell expression system previously reported, suggesting the recombinant HCV E2 protein derived from our Bacu system can be utilized for development of a subunit vaccine and diagnostics. Interestingly, HCV E2 protein was not degraded at all even at 43 h post-heat shock in the heat shock-induced necrotic cells, probably due to its integration into the microsomal membrane, indicating that heat shock can be employed to purify HCV E2 protein.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.30 no.1
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• pp.11-21
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• 2008

Aim of the study: Thermal stress is a central determinant of osseous surgical outcomes. Interestingly, the temperatures measured during endosseous surgeries coincide with the temperatures that elicit the heat shock response of mammalian cells. The heat shock response is a coordinated biochemical response that helps to protect cells from stresses of various forms. Several protective proteins, termed heat shock proteins (hsp) are produced as part of this response. To begin to understand the role of the stress response of osteoblasts during surgical manipulation of bone, the heat shock protein response was evaluated in osteoblastic cells. Materials & methods: With primary cell culture studies and ROS 17/2.8 osteoblastic cells transfected with hsp27 encoding vectors culture studies, the thermal stress response of mammalian osteoblastic cells was evaluated by immunohistochemistry and western blot analysis. Results: Immunocytochemistry indicated that hsp27 was present in unstressed osteoblastic cells, but not fibroblastic cells. Primarily cultured osteoblasts and fibroblasts expressed the major hsp in response to thermal stress, however, the small Mr hsp, hsp27 was shown to be a constitutive product only in osteoblasts. Creation of stable transformed osteoblastic cells expressing abundant hsp27 protein was used to demonstrate that hsp27 confers stress resistance to osteoblastic cells. Conclusions: The demonstrable presence and function of hsp27 in cultured bones and cells implicates this protein as a determinant of osteoblastic cell fate in vivo.

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.369-373
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• 2003

In spite of potential benefits of anther culture, low productivity of plant regeneration in some genotypes; e.g. tonsil and indica rice, is one of the major obstacles for practical use of anther culture. This study was conducted to improve cold shock method and carbohydrate source for increasing the efficiency of anther culture in rice. The most common carbon source, sucrose was replaced to maltose, which has two molecules of glucose. Maltose increased callus induction 1.4-to 1.8-fold higher in japonica rice, 3.2-to 11.6-fold in tongil types and 2.7-fold in indica rice IR50. Callus induction was increased from 0.2％ to 12.5％ in maltose medium compared to the medium supplemented with sucrose plus glucose in indica rice "Tetep". A simple procedure of vacuum packaging of panicles during cold shock treatment prolonged not only anther viability more than 15 days but also increased callus induction more than 2-fold compared to open-air storage (conventional method). Combining of above two methods, callus induction was increased 28 to 56％ in japonica, 13 to 33％ in tonsil type and 12 to 31％ in indica rice. Plant regeneration was increased 14 to 35％ in japonica, 10 to 20％ in tonsil and 4 to 15％ in indica rice, respectively.

• Convergence Security Journal
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• v.12 no.5
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• pp.51-59
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• 2012

Purpose of this study analyzes deeply and examine closely effect that incident shock of private guards gets in job stress. Used participation observation and In-depth analytical method to 10 common people guards inhabiting in capital region in 2012 to achieve purpose of this study. Collected interview data encoded using computer and analyzed by incident shock, job effect, job stress, organizational life. Conclusion proved in this study is as following. First, private guards appeared that incident shock such as suicide, terror threat, irrationality of superior that is produced in business spot gives great psychological fear. Second, private guards appeared suffer important shock are influenced in fear about business from shock that suffered in the past, and sociophobia appeared by thing which exert important effect changing by other business territory. Third, most private guards appeared that oneself experienced job stress from experiencing incident shock in the past. That is, incident shock exerts direct influence on customer confrontation in the past. Fourth, private guards appeared by thing which individual's organization life reduces when do not overcome incident shock that occur in the past. That is, incident shock decreases trust development and teamwork between superior and subordinate in organizational culture of private security.

• Korean Journal of Plant Tissue Culture
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• v.24 no.5
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• pp.295-303
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• 1997

To investigate the response on feeder cell cultures, protoplasts isolated from cell suspensions initiated from mature seed scutellum-derived callus of the Japonica rice variety Taipei 309, were cultured on filter membranes under various conditions. The effects of various factors, such as gelling agents, feeder cell and protoplast densities, species of feeder cells and heat shock treatment, have been investigated to improve protoplast plating efficiencies on filter membranes. Maximum protoplast plating efficiencies were obtained when protoplasts were cultured on KPR medium semi-solidified with Sea Plaque agarose at a density of $5\;\times\;10^{5}\;ml^{-1}$ protoplasts in the presence of Lolium multiflorum as feeder cells (0.5 ml pcv per 10 ml of protoplast culture medium). Pre-culture heat shock treatments for 1 min. and 5 min. to the protoplasts did not give any appreciable increase on the plating efficiency of protoplasts in the presence of feeder cells. Maltose-supplemented medium was superior for plant regeneration from protoplast-derived colonies compared with medium containing only sucrose. The plants were transferred to the glasshouse, flowered and were fertile.

• Journal of Veterinary Clinics
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• v.28 no.1
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• pp.75-80
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• 2011

Proteomics is a useful approach to know protein expression, post-translational modification and protein function. We investigated the protein expression pattern and identity in pigs fed with the tissue culture medium waste after harvest of Korean wild ginseng (TCM-KWG) (Panax ginseng). Two groups (n = 30/group) of pigs were administered with 0 (control) and 16 ml/L (treatment) TCM-KWG through drinking water. After 4 weeks, we examined the protein expression pattern of longissimus dorsi muscle by Two-dimensional electrophoresis analysis. TCM-KWG treatment significantly increased two spot's density, and markedly reduced one spot's density in the muscles. We identified 3 proteins (heat shock protein 90-alpha, myosin binding protein and cofilin 2) by the ESI-MS/MS (Q-TOF2, Micromass). These results demonstrate that TCM-KWG treatment may play a protection role against physiological stress in pigs, like as increased heat shock protein 90-alpha.