• Development and Reproduction
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• v.24 no.3
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• pp.225-230
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• 2020

This study reports the presence of mature and bi-sexual phase gonads in red spotted grouper, Epinephelus akaara after less than a year of cultivation in a commercial indoor tank and a net cage. In December 2018, juveniles were placed in an indoor tank and cultured for five months. In June 2019, the fish were transferred to a net cage and cultured until September. The rearing temperatures ranged from 19.86℃-24.65℃ in the indoor tank and 21.86℃-27.65℃ in the net cage. During the net cage culture period, specimens were randomly selected for histological gonad examination. The highest gonadosomatic index (GSI) value was measured in July (3.38±2.53), and dramatically decreased in August (0.44±0.21) and September (0.42±0.30). In July, some mature fish showed signs of vitellogenic stage oocyte development (vitellogenic and oil droplet stage oocytes), but immature fish were in an early developmental stage containing peri-nucleolus stage (PNS) oocytes. Bi-sexual phase gonads containing spermatocytes and spermatids were observed in the lumen and several PNS oocytes. By August and September, most specimens showed early-stage ovary development. However, mature testis (in August) and bi-sexual phase gonads (in September) were also observed. These results provide evidence for early puberty and hermaphroditism in the red spotted grouper.

• Journal of Microbiology
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• v.37 no.4
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• pp.200-205
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• 1999

7-Aminocephalosporanic acid (7-ACA) is the initial compound in preparation of cephalosporin antibiotics widely used in clinical treatment. Bacteria producing glutaryl 7-ACA acylase, which convert cephalosporin C to 7-ACA, has been screened in soil samples. A bacterial strain exhibiting high glutaryl 7-ACA acylase activity, designated KAC-1, was isolated and identified as a strain of Pseudomonas diminuta by characterizing its morphological and physiological properties. The screening procedures include culturing on enrichment media containing glutaric acid, glutamate, and glutaryl 7-aminocephalosporanic acid as selective carbon sources. To enhance enzyme production, optimal cultivation conditions were investigated. This strain grew optimally at pH 7 to 9 and in temperatures of 20 to 40 C, but acylase production was higher when the strain was grown at 25 C. Glutaric acid, glutamate and glucos also acted as inducers for acylase production. In a jar fermenter culture, P. diminuta KAC-1 produce acylase in a growth-associated manner. The substrate specificity of KAC-1 acylase by cell extract showed that this enzyme had specificity toward glutaryl 7-ACA, glutaryl 7-ADCA, but not cephalosporin C.

• Korean Journal of Agricultural Science
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• v.48 no.3
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• pp.505-514
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• 2021

Among non-timber forest products, Tricholoma matsutake is a high value added item. Many countries, including Korea, China, and Japan, are doing research and technology development to increase artificial cultivation and productivity. However, the production of T. matsutake is on the decline due to global warming, abnormal temperatures and pine tree pest problems. Therefore, it is necessary to identify trends in domestic and foreign research on T. matsutake, respond to preemptive research and development to preserve the genetic resources of T. matsutake and increase its productivity. Based on the correlation between keywords in the high frequency keywords, it was observed that microbial clusters of T. matsutake are mainly found in Korea. The main focus in China has been the pharmacology studies on the ingredients of T. matsutake. The main focus in Japan has been on preserving the genetic diversity and species of T. matsutake. Thus, future domestic studies of T. matsutake will require pharmacological studies on the ingredients of T. matsutake and on its genetic diversity and species conservation. In addition, unlike China and Japan, genetic keywords did not appear in Korea at high frequency. Therefore, Korea will have to proceed with research using modern molecular biology techniques.

• KSBB Journal
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• v.27 no.1
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• pp.21-27
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• 2012

Five yeast strains were isolated from traditional Makgeollies, Makgeollies were made by isolated yeasts after cultivation, and then property changes of Makgeollies were analyzed according to yeasts, storage temperatures and storage periods. Average pHs were shown to be 3.22~3.88 and statistically changed according to yeasts used, storage temperatures and storage periods. Total acidities were statistically changed according to storage periods. Amino-type nitrogen contents were in the ranges of 0.009~0.245% and statistically changed according to storage temperatures especially at 18 and $25^{\circ}C$ for 15 days. Average alcohol concentrations were in the ranges of 7.5~18.5% and reduced until 10 days and increased for 15 days according to yeasts used and storage periods. Consequently, Makgeollies, made by isolated yeast strains originated from traditional Makgeollies, revealed that alcohol concentrations and amino-type nitrogen contents were changed but pHs and total acidities were not dramatically changed according to yeasts used. It suggests that development of various Makgeollies would be possible using isolated yeast strains in this study, and optimal storage condition of ready-made Makgeollies to maintain its original property turned out to be at $4^{\circ}C$ for 5 days. Especially, Makgeolli made by F strain showed the best quality on its property, therefore Makgeolli which maintains its property stably until 10 days when stored at $4^{\circ}C$ could be made using this strain.

• Korean Journal of Agricultural and Forest Meteorology
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• v.23 no.4
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• pp.349-355
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• 2021

The effect of night temperature was investigated on mango (Mangifera indica cv. Irwin) for setting appropriate night temperature and managing mango cultivation in a greenhouse. Different night temperatures (10, 15, and 20℃) were treated from budding to fruit development in mango trees. As night temperature increased, the initiation of flowering, full blooming, fruiting, and fruit development tended to be accelerated. There were no significant differences in the growth of shoots flushed after the fruit harvest among trees treated with different night temperatures. The lengths of the flower stalk were the shortest at 10℃ of night temperature; however, there were no significant differences between 15℃ and 20℃ of the night temperature. The length, diameter, and weight of mango fruits were higher at 15℃ and 20℃ than 10℃. Soluble solid contents and acidities showed no difference; the firmness and skin color were better as the night temperature increased. Accordingly, setting the minimum night temperatures to 15℃ or 20℃ can increase the number of fruits and produce high-quality fruits when cultivating mango in the greenhouse. Even the minimum night temperature to 15℃ can reduce heating costs in the greenhouse.

• Korean Journal of Agricultural and Forest Meteorology
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• v.20 no.2
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• pp.190-204
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• 2018

The probability of safe cropping and the major phenological stages in rice were assessed using daily mean temperature data from 1981 to 2016 at 27 sites in North Korea. The threshold temperatures for early marginal transplanting date (EMTD), marginal harvesting date (MHVD), safe marginal heading date (SMHD), and cumulative temperature-based heading date (CTHD) were set to be $14^{\circ}C$, $13^{\circ}C$, $22^{\circ}C$ for 40 days after heading, and cumulative temperature of $1200^{\circ}C$ to MHVD, respectively. The safe heading date (SHD) was assumed to be either SMHD or CTHD whichever was earlier. It was also assumed that the minimum requirement for the suitability of safe rice cropping was met when both SMHD and CTHD appeared along with the time period of 60 days or more from EMTD to SHD. It was analyzed that 17 sites (Kaesong, Haeju, Yongyon, Singye, Sariwon, Nampo, Pyongyang, Anju, Kusong, Sinuiju, Changjon, Wonsan, Hamhung, Pyonggang, Huichon, Supung, Kanggye) had 90% or higher probability, two sites (Yangdok, Sinpo) had 80-90% probability, and eight sites (Kimchaek, Chunggang, Chongjin, Sonbong, Changjin, Pungsan, Hyesan, Samjiyon) had less than 80% probability of the safe rice cropping. For each region, the representative EMTD, SHD, and MHVD were analyzed using the 80 percentile of total years tested. The ranges for EMTD, SHD, and MHVD were May 4 in Sariwon~May 24 in Sinpo, June 21 in Kanggye~August 11 in Haeju, and September 17 in Kanggye~October 16 in Haeju and Changjon, respectively. Time durations from EMTD to SHD and from SHD to MHVD were 67~97 days and 57~72 days, respectively, depending on the regions. This study would facilitate modeling efforts for rice yield simulation in future studies. Our results would also provide basic information for practical researches on the rice cropping system in North Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.4
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• pp.304-310
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• 2017

This study was conducted over a 3-year period from 2013 to 2015 in the mid-northern inland, Cheolweon, Korea, to investigate changes in flowering date, daily mean temperature during grain filling, and yield characteristics affected by transplanting date in an early-maturing rice variety, 'Joun'. Thirty-day-old seedlings were transplanted at four different dates at 15-day interval from May 5 to June 19. Flowering dates were July 16, July 21, July 31, and August 14 when transplanting was performed on May 5, May 20, June 4, and June 19, respectively. Late transplanting resulted in higher daily mean temperature before flowering but late-transplanted rice required fewer days and lower cumulative temperature to reach flowering from transplanting. As transplanting was delayed, daily mean temperature for 40 days after flowering decreased, whereas daily sunshine hours for the same period increased, with a temperature of $24.8^{\circ}C$ and sunshine for 5.8 hours being recorded at the transplanting on May 5, and with a temperature of $21.0^{\circ}C$ and sunshine for 7.7 hours at the transplanting on June 19. With late transplanting, panicles per square meter significantly decreased, whereas spikelets per panicle showed an increasing trend. Regression analysis showed that maximum head rice yield was attained from the transplanting on May 18, for which the flowering date was July 21, and daily mean temperature for 40 days from that flowering date was $24.6^{\circ}C$. A decrease in head rice yield by 5% and 10% of the maximum was observed for rice transplanted on June 6 and June 15, which resulted in flowering dates of August 2 and August 11, respectively, and the daily mean temperatures for 40 days from flowering were 23.2 and $21.7^{\circ}C$, respectively. Therefore, in mid-northern inland, it is recommended to transplant 'Joun' on May 18 to induce flowering on July 21, when grain filling is subjected to a daily mean temperature of $24.6^{\circ}C$ during active filling stage.

• Korean Journal of Environmental Biology
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• v.32 no.1
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• pp.58-67
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• 2014

The outdoor mass cultivation is not possible for microalgae in Korea all year round, due to cold winter season. It is not easy to maintain proper level of productivity of microalgae even in winter. To prevent a drastic decrease of temperature in a greenhouse, two layers were covered additionally, inside the original plastic layer of the greenhouse. The middle layer was made up of plastic and the inner layer, of non-woven fabric. Acrylic transparent bioreactors were constructed to get more sunlight, not only from the upper side but also from the lateral and bottom directions. In winter at freezing temperatures, six different culture conditions were compared in the triply covered, insulated greenhouse. Wastewater after anaerobic digestion was used for the cultivation of microalgae to minimize the production cost. Water temperature in the bioreactors remained above $10^{\circ}C$ on average, even without any external heating system, proving that the triple-layered greenhouse is effective in keeping heat. Algal biomass reached to 0.37g $L^{-1}$ with the highest temperature, in the experimental group of light-reflection board at the bottom, with nitrogen and phosphorus removal rate of 92% and 99%, respectively. When fatty acid composition was analyzed using gas-chromatography, linoleate (C18 : 3n3) occupied the highest proportion up to 61%, in the all experiment groups. Chemical oxygen demand (COD), however, did not decrease during the cultivation, but rather increased. Although the algal biomass productivity was not comparable to warm seasons, it was possible to maintain water temperature for algae cultivation even in the coldest season, at the minimum cost.

• Journal of Food Hygiene and Safety
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• v.33 no.6
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• pp.447-452
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• 2018

Water is an important component in the production of fresh produce. It is mainly used for irrigation and application of pesticides and fertilizers. Several outbreaks cases related to fresh produce have been reported and water has been identified as the most likely source. On the other hand, wildlife has been identified as a possible source of the waterborne pathogens. The purpose of this study was to investigate the effect of wildlife access on irrigation water used in the cultivation of Chinese cabbage in Goesan. The frequency of wild animals access to upstream water source and the contamination level of bacteria such as Escherichia coli and Enterococci of irrigation water used in Chinese cabbage farm was examined. A total of 37 wildlife including the wild bear and water deer were observed in upstream of water source during the cultivation of Chinese cabbage. The result indicated the presence of hygienic indicator bacteria from the upstream where there is no human access. The contamination range of coliforms, E. coli, and Enterococcus spp. Detected in the irrigation water were 2.13~4.32 log MPN / 100 mL, 0.26~2.03 log MPN / 100 mL, and 1.43~3.49 log MPN / 100 mL, respectively. Due to low water temperatures, the contamination levels of coliform bacteria and E. coli in the irrigation water during harvesting time was lower compared to those recorded during transplanting of Chinese cabbage. However, no significant difference was detected in the number of Enterococci during the cultivation of Chinese cabbage. The results indicated the need to manage the microbial risk in irrigation water to enhance safety in cultivation of Chinese cabbage.

• Korean Journal of Plant Resources
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• v.34 no.1
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• pp.64-72
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• 2021

This study aimed to identify regions with a suitable growth environment for Ligusticum chuanxing Hort. and use basic data to identify appropriate cultivation and stable production strategies. Four main areas of cultivation were selected and the relationship between growth characteristics (aboveground and underground parts) and weather and soil environment was analyzed. Overall growth was found to be significantly higher in Pyeongchang. Atmospheric and soil temperatures showed a significant negative correlation with overall height from the ground, stem diameter, and growth characteristics of the underground part; leaf length and width were positively correlated. As insolation increased, the growth characteristics, excluding leaf size, showed a positive correlation. Soil characteristics such as organic matter (OM), N, P, and K showed negative correlations with the overall height of the upper part and growth characteristics of the underground part, including stem diameter. Analysis of roots indicated that OM, N, P, and K were essential and were absorbed through the soil. The OM, N, P, and K values in the Pyeongchang area, which showed the optimal growth, were lower than those in other areas. It is believed that these results can be used to select cultivation sites for L. chuanxing and establish cultivation technology in future.