• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.39-39
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• 2017

In Asia, sweet potato (Ipomoea batatas) is a very important crop for starch production. Approximately 74.3% of the total sweet potato production quantity is produced in Asia (FAO, 2014) and China is the largest producer of sweet potato. Post-harvest management is particularly important because it is difficult to maintain the quality as well as quantity of sweet potatoes. Despite the importance of post-harvest management, researches on sweet potato have been focused on production-related study such as breeding of new variety, improved techniques of cultivation, so there is limited research on storage after harvest. Curing is a normal practice after sweet potato harvest to promote wound healing and extend postharvest storage life. In Korea, harvested sweet potatoes are usually cured for 4 to 7 days at $30-33^{\circ}C$ and 80-95% relative humidity within one week. Since the optimum storage temperature of sweet potato is regarded as $15-20^{\circ}C$, additional facilities and costs are required to raise the temperature for curing. However, the majority of small farmers do not have the capacity to provide additional facilities and costs. This study was initiated to suggest a new curing method to accelerate the wound healing by applying chemical oxidation to the wound surface of sweet potato. Oxidative stress is known to play an important role in the synthesis of secondary metabolites including lignin. In addition, chemical oxidation can be applied to prevent spoilage caused by microorganisms. Powerful gaseous oxidant with excellent penetration ability and superior sterilization effect was selected for this study. Lignification, weight loss, and spoilage rate of artificially wounded sweet potatoes were investigated after oxidant fumigation. There were clear differences in morphological analysis such as lignification pattern, lignin deposition color, and continuity of lignified cell layers between oxidant-fumigated sweet potatoes and control. These results show that gaseous oxidant can be used to supplement or replace the curing practice, to improve shelf-life as well as curing cost reduction.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.6
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• pp.53-59
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• 2012

This this study was conducted with the aim of doing experiment on the effect of water purification by using an artificially built plant island, which is one of the eco-techniques, and aquatic plants as a plan for the reuse of water for obtaining water resources, thereby analyzing the removed quantity, and applying the experimental results to the reuse of water. As a result of doing experiments, this study obtained a good measured value of BOD (biochemical oxygen demand) 4.7mg/L, and COD (chemical oxygen demand) 7.2mg/L below the heavy water standard of BOD 10mg/L and COD 20mg/L, respectively. The chromaticity showed 89.2% removal efficiency, but final treated wastewater was found to show chromaticity 58 degrees exceeding chromaticity 20 degrees which are the water quality standard of the reuse of water. The results revealed that T-N produced 27% removal efficiency on an average while T-P produced 38% removal efficiency on an average, showing that the removal effect of N & P wasn't big. According to the currently enforced "Water Quality Standard of Heavy Water by Use", the use of water for sprinkling and landscaping was found to be available. Accordingly, this study suggested a nature-friendly, economically-efficient, and eco-technological water treatment technique which will make it possible to overcome the limit of the existing physio-chemical water treatment technology, reduce the costs for maintenance and facilities, and also reduce the limit of space restraint for installation of facilities.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.1
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• pp.43-51
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• 2002

The cloning and expression of $\beta-glucosidase$ II, encoded by the gene ${\beta}glu2$, from thermotolerant yeast Pichia etchellsii into Escherichia coli is described. Cloning of the 7.3 kb BamHI/SalI yeast insert containing ${\beta}glu2$ in pUC18, which allowed for reverse orientation of the insert, resulted in better enzyme expression. Transformation of this plasmid into E. coli JM109 resulted in accumulation of the enzyme in periplasmic space. At $50^{\circ}C$, the highest hydrolytic activity of 1686 IU/g protein was obtained on sophorose. Batch and fed-batch techniques were employed for enzyme production in a 14 L bioreactor. Exponential feeding rates were determined from mass balance equations and these were employed to control specific growth rate and in turn maximize cell growth and enzyme production. Media optimization coupled with this strategy resulted in increased enzyme units of 1.2 kU/L at a stabilized growth rate of $0.14\;h^{-l}$. Increased enzyme production in bioreactor was accompanied by formation of inclusion bodies.

• Food Science of Animal Resources
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• v.32 no.2
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• pp.241-246
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• 2012

This report outlines the development of a rapid, simple, and sensitive detection system for pathogenic bacteria using a capillary electrophoresis-based, single strand conformation polymorphism (CE-SSCP) combined with PCR. We demonstrate that this method, used with primers targeting the V4 region of the16S rRNA gene, is capable of the simultaneous detection of 10 microbes that could be associated with foodborne illness, caused by animal-derived foods: Salmonella enterica, Listeria monocytogenes, Escherichia coli O157:H7, Campylobacter jejuni, Staphylococcus aureus, Bacillus cereus, Clostridium perfringens, Yersinia enterocolitica, Vibrio parahaemolyticus, and Enterobacter sakazakii. The traditional detection techniques are time-consuming and labor-intensive, due to the necessary task of separate cultivation of each target species. As such, the CE-SSCP-PCR method, that we have developed, has the potential to diagnose pathogens rapidly, unlike the traditional technique, in order to prevent foodborne illness in a much more efficient manner.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.40 no.5
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• pp.315-322
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• 2007

Kjellmaniella crassifalia Miyabe is one of the valuable seaweed crops cultured in the Korean coastal waters of the East Sea. Unfortunately recent environmental changes have adversely influenced its growth, prompting the need to develop techniques for species restoration. To obtatin biological information, the growth of the species was monitored for one year. The maximum blade length, $110{\pm}45cm$, was attained in July, and zoospores were released in November. The optimum culture conditions allowing juvenile sporophytes to grow to young blades consisted of $20{\mu}mol/m^2s\;at\;10^{\circ}C$ under which the blade length grew to $1,732.5{\pm}143.3{\mu}m$ in 42 days. Sporangial sori started to form in September, reaching 85% maturity in November and 89% maturity in December. Examining the growth of the species according to depth, the individuals growing at 5 meters grew the most, reaching a blade length of $75.9{\pm}18.9cm$ in August.

• Journal of Mushroom
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• v.3 no.4
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• pp.159-171
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• 2005

The supply and demand of mushrooms has increased dramatically as the standard of living has increased and the nutritional and medical value of the mushrooms has been discovered. However, in meeting the demand, the profitability of the mushroom is being reduced. Even though there are many different types of and uses for mushrooms, the overall output production for the last ten years has consistently increased 5.9% per year, and for the last five years('00~'04) 5.2 % per year, and for the last 3 years('02~'04) 2.9% per year- the rate of increase has slowed. Looking at the standard gross income from mushroom production, it has increased 5.2% over the last nine years('95~'03), 1.6% over the last 4 years('00~'03), and 3.2% over the last 3 years('02~'04)-the annual gross income is decreasing. Even though the farmers are producing more mushrooms, the annual gross income is not increasing enough to keep up with the production, and therefore the profitability of mushrooms is decreasing. Since 2002, the constant increase of the output of edible mushrooms such as the Common Mushroom, Oyster Mushroom, King Oyster Mushroom and Winter Mushroom has resulted in a price declines. Before 2004, Korea mainly exported mushrooms, but after 2004 Korea mainly imported mushrooms. The mushroom industry of Korea is nearing the end of the stage of 'supplying domestic demand.' The stages are as follows: demand raised ${\rightarrow}$ supply domestic demand ${\rightarrow}$ export ${\rightarrow}$ country with developed mushroom industry. Therefore, we should have an efficient system to promote the exportation of mushrooms. Not only the exportation of mushroom itself but also the exportation of mushroom spawn, cultivation techniques and processing facilities should be our goal when we formulate the policy.

• Korean Journal of Agricultural and Forest Meteorology
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• v.3 no.1
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• pp.37-43
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• 2001

Crop status monitoring and yield prediction at higher spatial resolution is a valuable tool in various decision making processes including agricultural policy making by the national and local governments. A prototype crop forecasting system was developed to project the size of rice crop across geographic areas nationwide, based on daily weather pattern. The system consists of crop models and the input data for 1,455 cultivation zone units (the smallest administrative unit of local government in South Korea called "Myun") making up the coterminous South Korea. CERES-rice, a rice crop growth simulation model, was tuned to have genetic characteristics pertinent to domestic cultivars. Daily maximum/minimum temperature, solar radiation, and precipitation surface on 1km by 1km grid spacing were prepared by a spatial interpolation of 63 point observations from the Korea Meteorological Administration network. Spatial mean weather data were derived for each Myun and transformed to the model input format. Soil characteristics and management information at each Myun were available from the Rural Development Administration. The system was applied to the forecasting of national rice production for the recent 3 years (1997 to 1999). The model was run with the past weather data as of September 15 each year, which is about a month earlier than the actual harvest date. Simulated yields of 1,455 Myuns were grouped into 162 counties by acreage-weighted summation to enable the validation, since the official production statistics from the Ministry of Agriculture and Forestry is on the county basis. Forecast yields were less sensitive to the changes in annual climate than the reported yields and there was a relatively weak correlation between the forecast and the reported yields. However, the projected size of rice crop at each county, which was obtained by multiplication of the mean yield with the acreage, was close to the reported production with the $r^2$ values higher than 0.97 in all three years.

• International Journal of Advanced Culture Technology
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• v.9 no.1
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• pp.58-63
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• 2021

This research aims to reconsider the necessity of an alternative way(s) for contemporary actor training and acting in discussing and articulating Diderot and Suzuki's concepts and approaches for acting/training. First of all, the physical body, assumed and conceptualized by Diderot is beyond our control by means of a type of radical body/mind dualism, and is based on the concept that body and mind are separate. In contrast, Suzuki's notion of acting/training is raised by his concern about the role of an actor's body in the constitution of an actor's bodily experience against the imitation of the West-oriented theatre/acting/training. The descriptions of the two theatre artists' notion of acting/training gives us insight into the place and role of contemporary theatre as a practical root to encounter and communicate between a doer and a spectator where an actor's body must appropriately be attuned and cultivated towards the cultivation of bodily attributes which are foundation but usually neglected by actors/directors/practitioners particularly in Korea. Especially, misunderstanding of a specific training sources/approaches, namely 'scientific system' and the 'method' have taken us away from the potential possibilities of the lived oneness. Here, the 'possibility' refers to the primary bodily functions within a specific context or being in the here and now rather than attempting to copying, imitating and/or adapting a specific cultural source(s)/approaches/techniques as we have faced with through the previous century. We reconsider and argue that a potential way to correspond the nature of theatre/acting/training is that how to meet the demand of contemporary spectators which in turn intensifies an actor's stability, sustainability and hopefully professional identity in this contemporary era.

• Journal of Plant Biotechnology
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• v.47 no.4
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• pp.324-329
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• 2020

Reed (Phragmites spp.) is a rhizomatous plant of the Poaceae family and is known as high tolerant plant to heavy metal contaminants. This plant is widely recognized as a Cd root-accumulator, but improved heavy metal tolerance and uptake capacity are still required for phytoremediation efficiency. To enhance capacity of hyperaccumulator plants, ethyl methane sulfonate (EMS) as chemical mutagen has been introduced and applied to remediation approaches. This study aimed to select EMS-mutagenized reeds representing high Cd resistance and large biomass and to investigate their ability of Cd accumulation. After 6 months cultivation of M₂ mutant reeds under Cd stress conditions (up to 1,500 µM), we discovered seven mutant individuals that showed good performances like survivorship, vitality, and high accumulation of Cd, particularly in their roots. Compared to wild type (WT) reeds as control, on average, dry weight of mutant type (MT) reeds was larger by 2 and 1.5 times in roots and shoots, respectively. In addition, these mutant plants accumulated 6 times more Cd, mostly in the roots. In particular, MT8 reeds showed the greatest ability to accumulate Cd. These results suggest that EMS mutagenesis could generate hyperaccumulator plants with enhanced Cd tolerance and biomass, thereby contributing to improvement of phytoremediation efficiency in Cd-contaminated soil or wastewater. Further studies should focus on identifying Cd tolerance mechanisms of such EMS-mutagenized plants, developing techniques for its biomass production, and investigating the practical potential of the EMS mutants for phytoremediation.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.27 no.1
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• pp.27-33
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• 2023

Demands for eco-friendly food materials are increasing rapidly because of increased interest in well-being and health care, deterioration of air quality due to fine dust, and various soil and water pollution. Aquaponics is a system that can solve various problems such as economic activities, environmental problems, and safe food provision of the elderly population. However, techniques for deriving the optimal growth environment should be preceded. In this paper, we intend to design an intelligent plant growth measurement system that considers the characteristics of existing aquaponics. In particular, we would like to propose a module configuration plan for learning data and judgment systems when providing a uniform growth environment, focusing on designing systems suitable for production sites that do not have high-performance processing resources among intelligent aquaponics production management modules. It is believed that the proposed system can effectively perform deep learning with small analysis resources.