• The Plant Pathology Journal
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• 제35권4호
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• pp.341-350
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• 2019

Streptomyces padanus PMS-702 strain produces a polyene macrolide antibiotic fungichromin and displays antagonistic activities against many phytopathogenic fungi. In the present study, experimental formulations were assessed to improve the production of fungichromin, the efficacy of PMS-702 on the suppression of sporangial germination, and the reduction of cucumber downy mildew caused by Pseudoperonospora cubensis. PMS-702 strain cultured in a soybean meal-glucose (SMG) medium led to low levels of fungichromin accumulation and sporangial germination suppression. Increasing medium compositions and adding plant oils (noticeably coconut oil) in SMG significantly increased fungichromin production from 68 to $1,999.6{\mu}g/ml$. Microscopic examination reveals that the resultant suspensions significantly reduced sporangial germination and caused cytoplasmic aggregation. Greenhouse trials reveal that the application of PMS-702 cultural suspensions reduced downy mildew severity considerably. The addition of Tween 80 into the synthetic medium while culturing PMS-702 further increased the suppressive efficacy of downy mildew severity, particularly when applied at 24 h before inoculation or co-applied with inoculum. Fungichromin at $50{\mu}g/ml$ induced phytotoxicity showing minor necrosis surrounded with light yellowish halos on cucumber leaves. The concentration that leads to 90% inhibition (IC90) of sporangial germination was estimated to be around $10{\mu}g/ml$. The results provide a strong possibility of using the S. padanus PMS-702 strain as a biocontrol agent to control other plant pathogens.

• 생물환경조절학회지
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• 제16권2호
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• pp.101-107
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• 2007

오이['장형흑진주' 오이; (주)서울종묘]를 공시작물로 하여 양질의 플러그묘 생산을 위한 혼합배지 개발과 적정 관비 양액 농도를 규명하기 위해 수행하였다. 혼합배지는 피트모스를 기본으로 왕겨, 훈탄, 부숙톱밥, 펄라이트 및 입상압면 등을 상이한 비율로 혼합하여 5처리로 하였고, 그 중 양호한 혼합배지 3종을 선발하여 관비 양액 농도(EC)를 대조구(EC $0.1dS{\cdot}m^{-1}$), 0.5, 1.0 및 $1.5dS{\cdot}m^{-1}$등으로 처리하여 오이 유묘의 생장반응(27일째)을 검토하였다. 오이 플러그묘 생장에 양호한 혼합배지는 피트모스:왕겨:훈탄:부숙톱밥:펄라이트=25:10:25:20:20(v/v), 피트모스 왕겨:부숙 톱밥:입상암면=30:25:20:25(v/v) 및 피트모스:왕겨:부숙톱밥=40:40:20(v/v)였다. 대조구 (EC $0.1 dS\;m^{-l}$)에 비해 관비 양액의 농도가 높아질수록 초장, 엽면적 및 총건물생산량 등이 현저히 높아 EC $1.5dS{\cdot}m^{-1}$로 두상관수 2-3회 관비하였을 때 가장 좋은 플러그묘를 생산할 수 있었다. 관비 양액 농도와 혼합배지 종류 처리간에는 유의차가 인정되지 않았다.

• 식물병연구
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• 제20권2호
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• pp.112-118
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• 2014

본 연구는 키틴분해세균, 키틴 및 그들의 산물이 함유된 미생물제가 오이의 뿌리혹선충을 어느 정도 방제할 수 있는가를 알아보기 위하여 실시되었다. 미생물제는 3종의 키틴분해세균(Chromobacterium sp. C-61, Lysobacter engymogenes 및 Serratia plymuthica)을 키틴 + 최소기본배지에서 배양함으로서 조제되었다. 포트 조건에서 미생물제는 대조구에 비하여 오이의 생육을 증진시키고, 뿌리혹과 뿌리혹선충의 밀도를 감소시켰다. 비닐하우스에서 정식한 날부터 물 대신에 75배 희석된 미생물제를 5일 간격으로 6회 오이에 점적 관수하면, 30일 후 오이의 초장이 7% 증가하고 근권의 뿌리혹선충 밀도는 78% 감소되며, 60일 후에는 10%의 초장 증가와 69%의 밀도 감소를 나타냈다. 아울러, 이러한 효과는 미생물제가 직접 접촉된 부분에서만 일어났다. 한편, 뿌리혹선충에 심하게 감염되어 생육이 저조한 오이에 미생물제 원액을 10일 간격 3회 토양 관주하였을 경우에는 무처리구에서 37%의 오이가 죽었지만 처리구에서는 한 주도 죽지 않고, 37%의 생육 증가와 82%의 선충 감소를 보여 주었다. 따라서 본 미생물제는 오이 뿌리혹선충의 방제에 활용 가능하리라 판단되었다.

• 한국식물병리학회지
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• 제14권1호
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• pp.46-51
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• 1998

A method for inoculum production of Botryosphaerisa dothidea was developed using cucumber disks and cereal media. Disks of cucumber fruits, and cereal media of barley, wheat, and rice seeds were inoculated with mycelial plugs of B. dothidea and incubated at 27$^{\circ}C$. Pycnidia were produced on the surface of cucumber disks and seeds after 5 days of inoculation. When the inoculated barley seeds were immersed in sterilized distilled water for 5 minutes, abundant conidia of B. dothidea were exuded from mature pycnidia. Conidia were held together by mucilage as they were released from an ostiole. Compared with the conventional method for inoculum preparation using agar media, such as potato-dextrose agar and oatmeal agar, this method could minimize the tedious work required for inoculum preparation within a shorter period of time.

• The Plant Pathology Journal
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• 제39권6호
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• pp.592-599
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• 2023

A defective RNA3 (D3Yα) of strain Y of cucumber mosaic virus (CMV-Y) was examined on host-specific maintenance, experimental conditions, and a viral factor required for its generation in plants. D3Yα was stably maintained in cucumber but not in tomato plants for 28 days post inoculation (dpi). D3Yα was generated in Nicotiana tabacum or N. benthamiana after prolonged infection in the second and the third passages, but not in plants of N. benthamiana grown at low temperature at 28 dpi or infected with CMV-Y mutant that had the 2b gene deleted. Collectively, we suggest that generation and retention of D3Yα depends on potential host plants and experimental conditions, and that the 2b protein has a role for facilitation of generation of D3Yα.

• 생물환경조절학회지
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• 제3권1호
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• pp.20-27
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• 1994

본 실험은 시설오이의 상품성 향상 및 생산성 증대를 위하여 주지형인 만능청장마디오이와 측지형인 사엽오이를 유인방법을 달리하여 재배하였으며 그 결과는 다음과 같다. 1. 유인방법 개선을 위한 오이의 저절위 착과특성을 조사하였던 바, 만능청장마디오이의 경우는 3마디까지, 사엽오이의 경우는 4마디까지가 수량 및 상품성이 불량하여 적과재배하는 것이 효과적이었다. 2. 오이의 유인방법 별 초기생육 및 과실의 개화시 자방중, 곡과도, 생장해석면에서 만능청장마디 오이는 횡유인, 종유인, 사면유인 순으로 좋았고 사엽오이는 횡유인, 사면유인, 종유인 순으로 좋게 나타났다. 3. 오이의 유인방법에 따라 초고별 상대조도와 적산엽면적지수로 본 작물의 수광태세는 만능청장 마디오이에서는 종유인과 횡유인이 사면유인보다 양호하였고 사엽오이는 횡유인, 종유인, 사면유인 순으로 나타났다. 4. 수량 및 품질은 만능청장마디오이와 사엽오이 모두에게 횡유인이 가장 우수하게 나타났다.

• Plant Breeding and Biotechnology
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• 제5권2호
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• pp.123-133
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• 2017

AP2/EREBP gene family consists of transcription factor genes with a conserved AP2 DNA-binding domain and is involved in various biological processes. AP2/EREBP gene families were identified through genome-wide searches in five Cucurbitaceae species including cucumber, wild cucumber, melon, watermelon, and bitter gourd, which consisted of more than 100 genes in each of the five species. The gene families were further divided into five groups including four subfamilies (ERF, DREB, AP2 and RAV) and a soloist group. Among the subfamilies, DREB subfamily which is known to be related to abiotic stress response was more analyzed and a total of 25 genes were identified as Cucurbitaceae homologues of Arabidopsis CBF/DREB1 genes which are important for abiotic stress-response and tolerance. In silico expression profiling using RNA-Seq data revealed diverse expression patterns of cucumber AP2/EREBP genes. AP2/EREBP gene families identified in this study will be valuable for understanding the stress response mechanism as well as facilitating molecular breeding in Cucurbitaceae crops.

• Journal of Plant Biotechnology
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• 제7권4호
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• pp.225-231
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• 2005

Five day old cotyledon explants of Cucumber (Cucumis sativus L) cv Poinsett 76 were cocultivated with two Agrobacterium strains (EHA105 and LBA 4404) each carrying GUS as the reporter gene and npt-II as the selection marker gene in the T-DNA region of the vector. Transformed shoots were selected at 150 mg/L kanamycin. A two day cocultivation coupled with $20\;{\mu}M$ acetosyringone increased the frequency (8.2 and 15.4 shoots) of GUS expression in the shoots of transformed plant. Among the two Agrobacterium strains, EHA 105 performed better than LBA 4404 in bringing two-fold increase in transformation efficiency (14%) than LBA 4404 (7.4%). PCR analysis was done to confirm the integration of T-DNA into cucumber genome.

• Agricultural and Biosystems Engineering
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• 제2권2호
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• pp.75-80
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• 2001

This paper describes method to diagnose abiotic stresses such ad low root temperature, low light intensity and high salinity in cucumber plants with several physiological instruments. The stresses could be detected by measuring and analyzing the differences in chlorophyll content, temperature difference between leaf and atmosphere and light absorptance at wavelengths of 480, 560, 710, 1420 and 1650nm. It was concluded that the stresses could be first diagnosed from the 3rd to 10th day after treatment and the overall accuracy of diagnosis was estimated between 25 and 75%. near-infrared spectrometer showed better and earlier detection than the other instruments investigated.

• Agricultural and Biosystems Engineering
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• 제2권1호
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• pp.1-6
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• 2001

This paper represents methods to diagnose nutrient deficient stresses of cucumber plants using several physiological instruments. The stresses could be detected by measuring and analyzing the difference of chlorophyll content, photochemical efficiency (Fv/Fm), temperature difference (DT) and light absorbance at wavelengths of 480nm, 560nm and 710nm between deficient and control plants. From the all over experiments, the stresses could be first diagnosed in the 3rd to 5th day in general after treatment and the overall diagnosis rate was estimated at more than 50% up to 100%.