• 통합자연과학논문집
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• 제16권3호
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• pp.97-102
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• 2023

The gasdermins are a family of recently identified pore-forming effector proteins that cause membrane permeabilization and pyroptosis, a lytic pro-inflammatory type of cell death. A role in the regulation of cell proliferation and/or differentiation is suggested by the differentiation status-specific expression of gasdermin proteins in epithelial tissues. One of the GSDM protein is Gasdermin A (GSDMA), which decreased in stomach and esophageal cancers, suggesting a tumor suppressor role. GSDMA receptor antagonists have been researched as potential treatments for inflammatory diseases and baldness. GSDMA's significance in a wide range of disorders makes it an important therapeutic target. As a result, homology modelling of the GSDMA receptor was undertaken in the current study using the crystal structures of Mus musculus (GSDMA3), Human gasdermin D (GSDMD), and Murine gasdermin D (murine GSDMD). The best model was chosen based on the validation results after 20 models were developed utilising single template-based approaches. The generated structures can be used for further binding site and docking studies in the future.

• Journal of Microbiology and Biotechnology
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• 제32권8호
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• pp.1034-1040
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• 2022

Fas-associated death domain (FADD) is an adapter molecule that bridges the interaction between receptor-interacting protein 1 (RIP1) and aspartate-specific cysteine protease-8 (caspase-8). As the primary mediator of apoptotic cell death, caspase-8 has two N-terminal death-effector domains (DEDs) and it interacts with other proteins in the DED subfamily through several conserved residues. In the tumor necrosis receptor-1 (TNFR-1)-dependent signaling pathway, apoptosis is triggered by the caspase-8/FADD complex by stimulating receptor internalization. However, the molecular mechanism of complex formation by the DED proteins remains poorly understood. Here, we found that direct DED-DED interaction between FADD and caspase-8 and the structure-based mutations (Y8D/I128A, E12A/I128A, E12R/I128A, K39A/I128A, K39D/I128A, F122A/I128A, and L123A/I128A) of caspase-8 disrupted formation of the stable DED complex with FADD. Moreover, the monomeric crystal structure of the caspase-8 DEDs (F122A/I128A) was solved at 1.7 Å. This study will provide new insight into the interaction mechanism and structural characteristics between FADD and caspase-8 DED subfamily proteins.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.546-549
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• 2000

Avidin-biotin의 강한 결합력을 이용하여 금속 표면 위에 리포좀과 같은 유기 분자막의 다층 형성 과정을 QCA의 공진주파수와 공진저항의 변화를 측정하므로서 실시간 모니터링의 가능성을 검토하고, 유기 분자막이 금속 표면 위에 적층 됨에 따라 형성되는 적층 막에 대한 정보를 수집함으로서 바이오센서 시스템으로 QCA를 적용 가능함의 기초 데이터를 제공하고자 한다.

• 한국미생물·생명공학회지
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• 제24권2호
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• pp.173-177
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• 1996

Bacillus thuringiensis subsp. morrisoni PG-14 is a gram-positive soil bacterium producing mosquitocidal parasporal inclusions composed of several crystal proteins. Among these crystal protein genes, cryIVD gene is one of major component which has 72 kDa in size. However, these parasporal inclusions sink quickly from the surface of water where mosquito larval feeding occurred. To develope mosquitocidal cyanobacterium, therefore, we constructed the expression vector, pCYASK 5-1 harboring cryIVD gene. The expression vector, pCYASK5-1 was transformed into the cyanobacterium Syne- chocystis PCC6803 reported as a natural mosquito larval food source and the transformants were selected with kanamycin. Expression of IVD gene in transformant was characterized by SDS-polyacrylamide gel electrophoresis (PAGE) and immunoblot analysis. The mosquitocidal activity of a transformant was determined with Culex tritaeniorhynchus. The results showed that, the transformed cyanobacterium is toxic to mosquito larvae and will be expected as a potential agent that is used for mosquito control.

• Journal of Ginseng Research
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• 제39권4호
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• pp.406-413
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• 2015

Background: Pathogenesis-related 10 (PR-10) proteins are small, cytosolic proteins with a similar three-dimensional structure. Crystal structures for several PR-10 homologs have similar overall folding patterns, with an unusually large internal cavity that is a binding site for biologically important molecules. Although structural information on PR-10 proteins is substantial, understanding of their biological function remains limited. Here, we showed that one of the PgPR-10 homologs, PgPR-10.3, shares binding properties with flavonoids, kinetin, emodin, deoxycholic acid, and ginsenoside Re (1 of the steroid glycosides). Methods: Gene expression patterns of PgPR-10.3 were analyzed by quantitative real-time PCR. The three-dimensional structure of PgPR-10 proteins was visualized by homology modeling, and docking to retrieve biologically active molecules was performed using AutoDock4 program. Results: Transcript levels of PgPR-10.3 expressed in leaves, stems, and roots of 3-wk-old ginseng plantlets were on average 86-fold lower than those of PgPR-10.2. In mature 2-yr-old ginseng plants, the mRNA of PgPR-10.3 is restricted to leaves. Ginsenoside Re production is especially prominent in leaves of Panax ginseng Meyer, and the binding property of PgPR-10.3 with ginsenoside Re suggests that this protein has an important role in the control of secondary metabolism. Conclusion: Although ginseng PR-10.3 gene is expressed in all organs of 3-wk-old plantlets, its expression is restricted to leaves in mature 2-yr-old ginseng plants. The putative binding property of PgPR-10.3 with Re is intriguing. Further verification of binding affinity with other biologically important molecules in the large hydrophobic cavity of PgPR-10.3 may provide an insight into the biological features of PR-10 proteins.

• KSBB Journal
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• 제15권5호
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• pp.497-500
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• 2000

Avidin의 금속표변에 대한 강한 결합력과 avidin-biotin의 강한 결합력을 이용하여 금속 표면 위에 라포좀과 같은 유기 분자막의 다층 형성 과정을 수정진동자를 이용하여 분석하였다. 금속 표변위에 적층되는 유기 분자막에 대한 정보를 수 집하고 그 가능성을 검토하여 바이오 센서에서 감도를 향상 시킬 수 있는 새로운 방법을 제시하고자 하였다.

• 한국결정성장학회지
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• 제23권1호
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• pp.1-7
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• 2013

중성자회절을 이용하여 단백질 내의 수소원자 위치를 포함한 3차원 상세구조를 분석할 수 있으므로, 중성자 회절용 단백질 단결정을 성장시키기 위해 상용자석을 이용한 자기장 단결정 성장장치를 개발하였다. 이 장치를 이용하여 중성자 회절실험에 필요한 1 $mm^3$ 이상 부피의 lysozyme 단결정 시료를 성장시켰다. 자기장 영향 하에서 성장시킨 단결정은 자기장의 영향 없이 성장한 시료보다 평균 부피가 크고 결정도가 우수하였으며, X-선 측정 결과에서도 해상도가 높고 작은 mosaicity를 나타내었다.

• 한국잠사곤충학회지
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• 제35권2호
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• pp.129-133
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• 1993

Bacillus thruingiensis var. kurstaki HD-1의 내독소 단백질 유전자의 발현기작을 규명하기 위하여 이 균으로부터 내독소 단백질 유전자가 존재하는 것으로 확인된 29Md와 44Md plasmid를 분리한 후, Sau3AI 제어효소로 부분절단하고, pBR322 BamHI site에 ligation하여, E. coli HB101 strain에 transformation시켜, 3,000여개의 Ampr/Ters한 colony를 얻어 면역학적 방법과 살충적 검정으로 통해 재조합 균주 KC1을 얻었다. PKC1 plasmid DNA는 vector DNA를 포함하여 약 12kb 정도의 크기를 가지며, B. t k HD-1 내독소 단백질과 이동도가 같거나 일치하는 132kd, 117kd의 KC1 specific band 2개를 얻었다. KC1 cell extract를 첨식한 결과 약 80% 치사율을 보였다.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2002년도 제9회 학술 발표회 프로그램과 논문초록
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• pp.29-29
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• 2002

In response to fungal invasion and other signals, plants accumulate a number of proteins that are involved in defense against pathogens. Osmotin is a 24 kDa protein belonging to the pathogenesis-related (PR) protein, a component of the hypersensitive response in leaves of tobacco plants exposed to tobacco mosaic virus.(omitted)

• 한국진공학회지
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• 제14권3호
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• pp.138-142
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• 2005

액상 x-선 소각산란법을 이용하여 단백질의 구조를 분석하였다. 사용한 단백질은 구조가 이미 알려진 Lysozyme과 $Bcl-XL(\vartriangle TM/\vartriangle loop)$ 그리고 $Bcl-XL(\vartriangle TM/\vartriangle loop))$에 자유롭게 움직이는 고리를 가진 $Bcl-XL(\vartriangleTM))$이다. Lysozyme와 $Bcl-XL(\vartriangle TM/\vartriangle loop)$에 대한 소각산란결과는 단백질 결정학으로부터 알려진 분자구조에서 얻은 이론적인 결과와 농도에 의한 차이정도를 제외하고는 잘 일치하였다. $Bcl-XL(\vartriangleTM))$의 경우는 단백질 결정산란 신호에서 볼 때 $Bcl-XL(\vartriangle TM/\vartriangle loop)$와 차이가 없는 것으로 알려져 있으나, 소각산란에서는 뚜렷한 차이를 나타내는 결과를 얻어 loop와 같이 쉽게 움직이는 부분을 가진 단백질을 연구하는 경우 소각산란의 장점을 확인할 수 있었다. 위 실험을 통하여 포항 가속기 연구소 4C1 빔라인의 성능은 적어도 해상도 $\sim2.2\;nm$까지 용액상의 단백질 구조를 분석할 수 있다는 것을 확인하였다.