• 한국식품영양과학회지
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• 제24권1호
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• pp.154-159
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• 1995

Inhibition of xanthine oxidase by tea extracts obtained from non-fermented tea(steamed green tea and roasted green tea), semi-fermented tea(oolong tea) and fermented tea(black tea) were investigated. The crude catechin fraciton had a hgher inhibitory effect against xanthine oxidase, and the effect was increased with the addition of tea extracts. Their inhibitory effect were hardly influenced until extracted three times with hot water. According to the investigation of catechins in the crude catechin fraction obtained from tea extracts, (-)-epicatechin-(EC), (-)-epicatechin gallate(ECg). (-)-epigallocatechin(EGC) and (-)-epigallocatechin gallate(EGCg) were 80.1$\mu\textrm{g}$/mg 113.5$\mu\textrm{g}$ /mg, 186.3$\mu\textrm{g}$/mg and 367.7$\mu\textrm{g}$/mg in steamed green tea, and 75.6$\mu\textrm{g}$/mg, 114.7$\mu\textrm{g}$/mg, 193.7 $\mu\textrm{g}$/mg and 381.9$\mu\textrm{g}$/mg in roasted green tea, and 69.4$\mu\textrm{g}$/mg, 110.0$\mu\textrm{g}$/mg, 127.1$\mu\textrm{g}$.mg and 464.9$\mu\textrm{g}$/mg in oolong tea, and 78.1$\mu\textrm{g}$/mg, 171.8$\mu\textrm{g}$/mg, 80.7$\mu\textrm{g}$/mg and 51.4$\mu\textrm{g}$/mg in black tea, respectively. Order of the content of these catechins was (-)-EGCg>(-)-EGC>(-)-ECg>(-)-EC in steamed green tea, roasted green tea and oolong tea, and was (-)-ECg>(-)-EGC>(-)-EC>(-)-EGCg in black tea. Also the concentration of catechins was hardly influeced until extracted three times. The inhibition ratio of xanthine oxidase by autherntic catechins was hardly influenced until extracted three times. The inhibition ratio of xanthine oxidase by authentic catechins was 94.9% and 87.6% by addition of 5.0$\mu\textrm{g}$/ml of (-)-EGCg and (-)-ECg, respectively. the inhibitors of xanthine oxidase were supposed to be due to (-)-ECg and (-)-EGCg in tea polyphenol compounds.

• Preventive Nutrition and Food Science
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• 제2권2호
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• pp.138-143
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• 1997

Crude catechin extracts were prepared using ethyl acetate from Korean Puerariae Radix (PR) and their biological effects on the alcoholic liver damage were investigated in male Sprague-Dawley rats (6 weeks old). Ethanol (5g ethanol/kg body weight/day) administration was performed for 8 weeks and after ethanol consuming rats were treated with one or two% catechin extracts of diet for 8 weeks. At the end of experimental period, lipid hydroperoxides in liver were analyzed using a chemiluminescence-high performance liquid chromatography (CL-HPLC) method. Compared with control animals, ethanol consumed rats showed lighter body weights, lower ratios of liver/body weight, higher activities of GOT and GPT, and increased lipid hydroperoxide amount in liver. With one or two% catechin extracts treatment, GOT and GPT activities returned to normal ranges. Lipid hydroperoxide contents in liver of on or two% PR treated rats lowered to 20% or 25% respectively, compared with the levels of those ethanol consumed animals without catechin extracts treatment. Therefore, we concluded that on or two% PR crude catechins treatment could be effective for alcoholic liver damage caused by lipid peroxidation.

• 약학회지
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• 제36권3호
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• pp.205-211
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• 1992

A simple dignostic method using Fast Atom Bombardment mass spectrometry was applied to the characterization of green tea flavonols from the eluates of Sepahadex LH-20 column chromatography. From the ethyl acetate extracts, crude mixture of flavonol fraction(Fr.$1{\sim}4$) were separated by the stepwise gradient elution with 30, 45, and 60% aqueous acetone. Procyanidine B analogues were found to be typical constituents of Fr. 1. Main components of Fr. 2 were catechins and gallo-catechins. Fr. 3 contained mainly ester type compounds, catechin-gallates, gallocatechin-gallates with their analogues. Fr. 4 was contaminated with some phthalate esters.

• 생명과학회지
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• 제6권2호
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• pp.104-110
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• 1996

The present study was conducted to elucidate the functional property of tea extract obtained from tea extracts, semi-fermented tea(oolong tea) and fermented tea(black tea). Tea extracts exhibited remarkable nitrite-scavenging actions, and the action increased with the increased of the amount of tea extracts. The nitrite-scavenging actionof tea extracts showed pH dependent, highest at pH 1.2 and lowest at pH6.0. The nitrite-scavenging rate in tea extracts/amine/nitrite systems proved to be faster than that in amine/nitrite systems. To screen the nitrite-scavenging factors, tea extracts were fractionated into water-soluble, methanol-soluble, methanol-precipitate and crude catechin fraction. Among these fractions of tea extracts, the crude catechin fraction possessed greater nitrite-scavenging action than the other fractions. The nitrite-scavenging action of tea extracts increased with the contents of total phenols and an absorbance at 280nm, nitrite-scavenging factors were supposed to be and catechins in tea polyphenol compounds.

• 한국식품저장유통학회지
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• 제15권4호
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• pp.576-581
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• 2008

울릉도산 산채인 울릉미역취, 부지갱이 및 삼나물의 식품학적 가치평가와 활용증대를 위한 기본 자료를 얻기 위하여, 건조 산채분말의 일반성분, 유기산, 유리 아미노산, riboflavin, thiamin, 지방산, 카테킨 및 미네랄 성분 함량을 각각 분석하였다. 산채류의 일반성분으로 수분함량은 $3.77{\sim}5.72%$, 조단백질은 $15{\sim}29%$, 조지방은 $3.50{\sim}6.68%$, 조섬유질은 $4.00{\sim}6.01%$, 조회분은 $8.70{\sim}10.54%$를 각각 나타내었다. 유기산의 종류와 함량은 산채별 차이가 있었으며 succinic acid, citric acid, malonic acid 등이 비교적 많이 함유되어 있었다. 유리 아미노산은 glutamic acid, aspartic acid, arginine, phenylalanine 등의 함량이 높았으며 품목별 차이는 크게 나타나지 않았다. Riboflavin 함량은 삼나물에서 약 90 mg%, 다른 산채는 $2{\sim}3mg%$이었고, thiamin 함량은 부지갱이에서 113 mg%, 삼나물에서 85 mg%, 울릉미역취에서 71 mg%를 나타내었다. 지방산은 linolenic acid, linoleic acid, palmitic acid 등의 함량이 높았고 이중 linolenic acid가 전체 지방산의 약 60% 정도를 차지하였다. 카테킨류는 삼나물에서 5.37 mg%, 울릉미역취에서 2.46 mg%, 부지갱이에서 1.29 mg%를 각각 나타내었고 삼나물의 주된 카테킨은 (-) EGC이었으며 다른 산채에서는 검출되지 않았다. 미네랄 성분은 Ca, P, Na의 함량이 비교적 높게 나타났다.

• 한국식품위생안전성학회지
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• 제13권4호
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• pp.377-382
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• 1998

항산화효과, 항돌연변이효과, 콜레스테롤농도 상승억제작용이 우수한 것으로 알려진 차추출물의 주요성분을 분리 정량하기 위하여 각 발효정도에 따른 분류 및 녹차의 채엽시기별에 따라 녹차 1번차 녹차2번차 녹차 3번차, 우롱차 및 홍차 등에 대한 일반성분, 아미노산, Vit.C, Vit. E, ${\beta}-carotene$, 카테킨류, 카페인, 데오브로마인 및 데오필린 등을 분석하고 특히 항산화성분인 카테킨류의 항산화효과 및 항돌연변이 효과의 연구에 기초자료로 이용하고자 하였다. 녹차 I, II, III, 우롱차 및 홍차의 일반 성분중 조지방함량은 각각 1.1, 2.5, 4.9, 0.8 및 1.2% 이었으며, 총 아미노산함량은 녹차I>녹차II>녹차III의 순이었고 대부분의 아미노산은 차 성분의 유일한 아미노산인 theanine이 차지하였다. 녹차 I, II, III, 우롱차 및 홍차에 함유된 Vit. C함량은 각각 101.6, 87.5, 95.9, 99.1 및 108.0 mg%이었으며, ${\beta}-carotene$ 함량은 각각 270, 268, 481, 80 및 181 ppm이었고, Vit. E 함량은 ${\alpha}-tocopherol$이 대부분을 차지하였으며 ${\beta}-$ 및 ${\delta}-tocopherol$은 모두 검출되지 않았다. 한편, 총카테킨류의 함량은 각각 10.5, 10.4, 7.2, 8.4 및 1.8%로 총카테킨류중 EGCG의 함량이 가장 높았다. 카테킨류 중 EGC의 함량은 녹차I>녹차III>녹차II>우롱차>홍차의 순으로 녹차I이 가장 높았으며, EGCG와 ECG의 함량은 우롱차>녹차I>녹차II>녹차III>홍차의 순으로 우롱차가 가장 높았다. 또한 GA함량은 0.01, 0.02, 0.05, 0.13 및 0.31%로 홍차에 가장 많은 것으로 나타났으며, 카페인 및 데오브로마인 함량도 홍차의 경우가 가장 높았으나 데오필린 함량은 녹차I이 가장 높게 나타났다.

• 한국축산식품학회지
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• 제23권2호
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• pp.180-185
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• 2003

본 연구는 운동부하와 DNA 및 칡 crude catechin의 급여에 의한 생체내 성분 변화를 알아보기 위하여 흰쥐를 대조군, 운동부하군, 운동부하+DNA 및 운동부하+DNA+crude catechin 첨가군으로 나누어 사육하였다. 각각 0.4% DNA와 0.1% 칡crude catechins을 해당 횐쥐에 급식시켰으며, 운동부하군은 Treadmill에서 20 m/min의 속도로 6주 동안 운동시켰다. 사육실험종료 후 체중 증체량과 체지방무게를 먼저 칭량한 후, 항산화활성과 체지방조성을 kit assay를 이용하여 측정하였다. 6주후 각 군간의 최종 체중에는 유의적인 차이가 없었으나 간장, 부고환주위지방 및 신장주위지방은 운동부하군, 운동부하+DNA 첨가군 및 운동부하+DNA+crude catechin 첨가군이 대조군에 비해 유의하게 낮은 값(p<0.05)을 나타냈다. 또, 혈중 phospholipid, cholesterol 및 triglyceride농도는 운동부하군, 운동부하+DNA 첨가군 및 운동부하+DNA+crude catechin 첨가군이 대조군에 비해 유의하게 낮았으나(p<0.05), HDL-cholesterol은 유의하게 높은 값을 나타냈다. GPT 활성은 각 군간에 유의차가 없었으나, GOT활성은 운동부하를 처리한 군에서 적은 수치를 나타냈으며, TBA가는 각 군간에 유의 차가 없었다. 또, 혈중 NEFA 수준은 운동부하+DNA+crude catechin 첨가군이 대조군에 비해 유의하게 높은 값을 나타냈다.(p<0.05). 이상의 실험에서 운동과 더불어 crude catechin 섭취가 유의한 차이를 나타내지는 않았지만 혈청지질의 성분의 향상에 보조역할을 할 것으로 사료된다.

• 한국축산식품학회지
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• 제18권4호
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• pp.348-357
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• 1998

This study was conducted to investigate the effects of mugwort extracts on the blood ethanol concentration and liver function in rats. Sprague-Dawley rats were used, the rats administered with 25% ethanol (5g/kg$.$B.W.) were devided into three groups (CON-E ; 25% ethanol administered to the CON-E) according to the administered ethanol concentration and the levels of administered mugwonts. Mugwont roots extracts were administered via the caudal vein. Ethanol concentration was measured at the time of 0, 1, 2 and 3hr by gas chromatography. GOT(Glutamic Oxaloacetic Transaminase) and GPT(Glutamic Pyruvic Transaminase) were measured at the time of 0 and 5hr. Components of each extracts were analyzed by using high performance liquid chromatography. Cell number, GOT and GPT were investigated by using rat hepatocyte culture. Megwort extracts were added at the levels of 1% or 2%. Hepatocyte culture were into five groups according to the addition levels. The results were summarized as follows ; 1. Catechin contents of 8∼10mg/100g and the contents of (-)-epigallocatechin was high in the water extracts. 2. Ethanol degradation efficiency declines in the following order : MDW-E＞MOH-E＞CON-E. 3. The numbers of rat hepatocytes declines in the following order : 2% MDW-L＞1%MDW-L＞1%MOH-L＞CON-L＞2%MOH-L. These results suggest that crude catechin of mugwort extracts may play important roles to degrade ethanol and recover liver function in rats.

• 한국식품조리과학회지
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• 제15권3호
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• pp.224-230
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• 1999

The purpose of this study was to find out the optimal mixing ratio of green tea powder, sugar, and water for the preparation of Seolgiddeok through Response Surface Methodology based on the color, sensory, and texture test. The oganoleptic and textural properties of Seolgiddeok prepared with various concentrations of green tea powder (0% (control group), 0.5% (GT-0.5 group), 1% (GT-1.0 group), 1.5% (GT-1.5 group), 2% (GT-2.0 group)), and their quality changes during storage were also investigated. The optimal mixing ratio of green tea powder, sugar, and water for preparing the best quality Seolgiddeok was 1.0%, 12%, and 22%, respectively. The proximate composition of green tea powder was 21.70% of crude protein, 8.49% of crude lipid, 2.95% of reducing sugar, and 6.40% of ash. The contents of crude lipid, reducing sugar, and catechins in Seolgiddeok added with a green tea powder were increased with increasing the content of green tea powder. The hardness and gumminess of GT-1.0 group were the lowest among four groups, whereas GT-1.0 and GT-2.0 groups had the lowest cohesiveness. While the control group was the lowest in adhesiveness, the springness was not significantly different among all groups. GT-0.5 and GT-1.5 groups were the highest in sweet taste and colorfulness, respectively. However, GT-1.0 group had the best overall quality. Total microbial numbers, the acidity and pH in Seolgiddeok during storage were decreased with increasing green tea powder content, and especially those of GT-1.0 and GT-1.5 groups were relatively the lower than others. The “L” value (lightness) of the control group (no additives) was the highest among five groups, and the value was decreased with storage period, and especially GT-0.5 groups had the lowest brightness. The“a”value (reddness) of the control group was the highest, followed by GT-0.5, GT-1.0, GT-1.5, and GT- 2.0 group in order. The“b”value (yellowness) was increased with the increase of green tea powder content. Above results indicated that GT-1.0 group showed the best quality of Seolgiddeok through organoleptic and rheology tests.

• 한국식품영양과학회지
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• 제37권2호
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• pp.190-194
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• 2008

팽이버섯 배지에 녹차 분말 또는 녹차 조카테킨 추출물을 농도별로 첨가하여 팽이버섯 자실체를 형성시킨 후 자실체의 이화학적 특성을 조사하였다. 버섯의 수확량은 무첨가구에서 최고의 수량을 보였으며 녹차분말 10% 첨가구에서 가장 낮은 값을 나타낸 반면, 경도는 무첨가구에서 가장 낮았고 녹차 분말 5% 및 10% 첨가구와 녹차 조카테킨 첨가구에서 높게 나타났다. 카테킨 및 카페인 함량 분석은 팽이버섯 추출물을 용매분획한 후 HPLC로 정량한 결과, 모든 시험구에서 카테킨은 검출되지 않았으며 총 폴리페놀 함량 또한 각 처리구간에 유의적 차이가 없는 것으로 나타나 카테킨 등 폴리페놀성 물질의 팽이버섯으로의 이행은 거의 없는 것으로 판단된다. 반면 카페인은 팽이버섯으로 이행되어 녹차 분말 무첨가구는 $2.12\;{\mu}g/g$, 녹차분말 10% 첨가구는 $196.23\;{\mu}g/g$이 검출되었다. 전자코를 이용한 향기패턴 분석 결과는 녹차 첨가에 의해 머무름 시간 3초와 6초 사이에 새로운 peak가 생성되었음을 알 수 있었으며, 녹차 10% 첨가구에서는 뚜렷한 향기의 변화가 있는 것을 확인할 수 있었다. 즉, 팽이버섯 배지에 녹차분말 또는 녹차 조카테킨 추출물 첨가에 의해 버섯의 경도가 증가되었으며 녹차 성분 중 카페인이 이행되어 새로운 기능성 버섯을 생산할 수 있을 것으로 사료되었다.